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The map label for this gene is tuf

Identifier: 124486257

GI number: 124486257

Start: 1473554

End: 1474831

Strand: Reverse

Name: tuf

Synonym: Mlab_1441

Alternate gene names: 124486257

Gene position: 1474831-1473554 (Counterclockwise)

Preceding gene: 124486259

Following gene: 124486256

Centisome position: 81.71

GC content: 52.35

Gene sequence:

>1278_bases
ATGGCAGCAGCAAAGCCCCACATGAATCTTGCCGTTATCGGTCACATTGACCACGGTAAGTCAACAACCGTTGGCCGGAT
TCTCTTCGAGACCGGTGTCGTACAGCAGCACATTCTTGATGGATACAAGAAAGAAGCAGAATCAAAAGGAAAAGCAACTT
TCGAGTTCGCCTGGGTAATGGACTCCCTCAAGGAAGAGCGTGAACGTGGTATTACCATCGATATCGCTCACAAGAAATTC
GAAACCCCGAAATACAATTTTACCGTTGTCGACTGTCCAGGTCACCGTGACTTCGTTAAGAACATGATCACCGGTGCATC
CCAGGCTGATGCAGCAATCATTGTCGTATCCGGAACCGAAGGCCCGATGGAACAGACCAAGGAACACGTTTTCCTGTCCA
AGACTCTTGGTATCAACCAGATCATCGTCGCCATCAACAAGATGGATGCAGTCAACTACTCCGAAGAGAAGTACAACGAA
GCAAAAGACAAGATGACCAAGCTCATCATGTCCGTTGGATTCAAACCGGCCGAGACCCCCTTCATCCCAATCAGCGCATT
CTGCGGTGACAACATTAAGGAAGCATCGGCAAACACCCCCTGGTACAAGGGCCCGACACTTCTTGCAGCACTTGATCTCT
TCAAGATGCCCGACATGCCGACCGACAAGCCGCTCAGACTCCCGATTCAGGATGTCTACACCATCTCCGGTGTTGGAACC
GTCCCAGTAGGCCGTGTTGAGACCGGTATCCTCAAGAAAGGACAGAAGATCTCCTTCATGCCGGCAAACGTTACCGGTGA
AGTTAAGTCCATTGAGATGCACCACGAAGAATTCCCCGAAGCACTTCCGGGAGACAACGTTGGTTTCAACGTCCGTGGTA
TCGCAAAGAACGATGTTCGCCGCGGCGATGTCTGTGGTCCGATCGAGAACCCGCCGACCGTTGCAGAGGAATTCACCGCA
CAGGTCGTCGTTCTCCAGCACCCGTCCGTCCTTTCCGTAGGATACACCCCGGTGTTCCACTGCCACACCTCACAGACTGC
ATGTATGTTCACCGAACTTAACAAGAAGCTGGACCCACGCTCCGGACAGGTTAAGGAAGAGAACCCGGCATTCCTCAAGG
CCGGCGATGCAGCAATCTGTACCATCACTCCGACCCGCCCGCTCGTCATTGAGACTGCAAAGGAACTCCCGCAGCTCGGC
AGATTCGCTGTCCGTGATATGGGTATGACCGTTGCAGCAGGTCTTGTTCTGAGCGTTAAAGCAAAGCAGATGAGATAA

Upstream 100 bases:

>100_bases
ATTTTATATGGTTGTAAGACCTATCTGTTTACACTCGTGTTGATTTGGATCATACTCTTTTGTACATCCGATCAATGAGT
CAAAGTGGAGATACATTATC

Downstream 100 bases:

>100_bases
TCTCATCGCTCACTCGAATTTTTTGTGGTGACTTAGAAAATGCAAAAAGCCAGAATACGCCTGACAGGGACTGATTATGA
GAAAGTGGAGACGGTATGTA

Product: elongation factor 1-alpha

Products: GDP; phosphate

Alternate protein names: EF-1-alpha; Elongation factor Tu; EF-Tu

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAAAKPHMNLAVIGHIDHGKSTTVGRILFETGVVQQHILDGYKKEAESKGKATFEFAWVMDSLKEERERGITIDIAHKKF
ETPKYNFTVVDCPGHRDFVKNMITGASQADAAIIVVSGTEGPMEQTKEHVFLSKTLGINQIIVAINKMDAVNYSEEKYNE
AKDKMTKLIMSVGFKPAETPFIPISAFCGDNIKEASANTPWYKGPTLLAALDLFKMPDMPTDKPLRLPIQDVYTISGVGT
VPVGRVETGILKKGQKISFMPANVTGEVKSIEMHHEEFPEALPGDNVGFNVRGIAKNDVRRGDVCGPIENPPTVAEEFTA
QVVVLQHPSVLSVGYTPVFHCHTSQTACMFTELNKKLDPRSGQVKEENPAFLKAGDAAICTITPTRPLVIETAKELPQLG
RFAVRDMGMTVAAGLVLSVKAKQMR

Sequences:

>Translated_425_residues
MAAAKPHMNLAVIGHIDHGKSTTVGRILFETGVVQQHILDGYKKEAESKGKATFEFAWVMDSLKEERERGITIDIAHKKF
ETPKYNFTVVDCPGHRDFVKNMITGASQADAAIIVVSGTEGPMEQTKEHVFLSKTLGINQIIVAINKMDAVNYSEEKYNE
AKDKMTKLIMSVGFKPAETPFIPISAFCGDNIKEASANTPWYKGPTLLAALDLFKMPDMPTDKPLRLPIQDVYTISGVGT
VPVGRVETGILKKGQKISFMPANVTGEVKSIEMHHEEFPEALPGDNVGFNVRGIAKNDVRRGDVCGPIENPPTVAEEFTA
QVVVLQHPSVLSVGYTPVFHCHTSQTACMFTELNKKLDPRSGQVKEENPAFLKAGDAAICTITPTRPLVIETAKELPQLG
RFAVRDMGMTVAAGLVLSVKAKQMR
>Mature_424_residues
AAAKPHMNLAVIGHIDHGKSTTVGRILFETGVVQQHILDGYKKEAESKGKATFEFAWVMDSLKEERERGITIDIAHKKFE
TPKYNFTVVDCPGHRDFVKNMITGASQADAAIIVVSGTEGPMEQTKEHVFLSKTLGINQIIVAINKMDAVNYSEEKYNEA
KDKMTKLIMSVGFKPAETPFIPISAFCGDNIKEASANTPWYKGPTLLAALDLFKMPDMPTDKPLRLPIQDVYTISGVGTV
PVGRVETGILKKGQKISFMPANVTGEVKSIEMHHEEFPEALPGDNVGFNVRGIAKNDVRRGDVCGPIENPPTVAEEFTAQ
VVVLQHPSVLSVGYTPVFHCHTSQTACMFTELNKKLDPRSGQVKEENPAFLKAGDAAICTITPTRPLVIETAKELPQLGR
FAVRDMGMTVAAGLVLSVKAKQMR

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG5256

COG function: function code J; Translation elongation factor EF-1alpha (GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI4503475, Length=444, Percent_Identity=51.5765765765766, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI4503471, Length=444, Percent_Identity=52.027027027027, Blast_Score=481, Evalue=1e-136,
Organism=Homo sapiens, GI5729864, Length=429, Percent_Identity=37.2960372960373, Blast_Score=312, Evalue=5e-85,
Organism=Homo sapiens, GI223555963, Length=429, Percent_Identity=37.2960372960373, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI194018522, Length=428, Percent_Identity=38.3177570093458, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI194097354, Length=428, Percent_Identity=38.3177570093458, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI194018520, Length=428, Percent_Identity=38.3177570093458, Blast_Score=306, Evalue=2e-83,
Organism=Homo sapiens, GI46094014, Length=428, Percent_Identity=38.3177570093458, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI34147630, Length=428, Percent_Identity=32.9439252336449, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI54607086, Length=369, Percent_Identity=27.10027100271, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI82546879, Length=376, Percent_Identity=24.2021276595745, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI157426893, Length=292, Percent_Identity=26.3698630136986, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4503507, Length=386, Percent_Identity=24.6113989637306, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790412, Length=421, Percent_Identity=34.2042755344418, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789737, Length=421, Percent_Identity=34.2042755344418, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1789108, Length=425, Percent_Identity=31.2941176470588, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI2367247, Length=386, Percent_Identity=25.6476683937824, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI48994988, Length=263, Percent_Identity=29.277566539924, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=262, Percent_Identity=25.9541984732824, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=442, Percent_Identity=51.5837104072398, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17569207, Length=442, Percent_Identity=51.5837104072398, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=49.4252873563218, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32566629, Length=430, Percent_Identity=35.1162790697674, Blast_Score=278, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI115532067, Length=434, Percent_Identity=35.9447004608295, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI115532065, Length=434, Percent_Identity=35.9447004608295, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI17556456, Length=424, Percent_Identity=33.9622641509434, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=51.6778523489933, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI25141371, Length=362, Percent_Identity=31.767955801105, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17506081, Length=307, Percent_Identity=25.0814332247557, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=249, Percent_Identity=29.3172690763052, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17565438, Length=382, Percent_Identity=22.2513089005236, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566299, Length=64, Percent_Identity=43.75, Blast_Score=71, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17509919, Length=363, Percent_Identity=23.6914600550964, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=439, Percent_Identity=52.1640091116173, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6319594, Length=439, Percent_Identity=52.1640091116173, Blast_Score=488, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6320377, Length=432, Percent_Identity=34.9537037037037, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6322937, Length=450, Percent_Identity=31.5555555555556, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6324761, Length=427, Percent_Identity=33.9578454332553, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6323320, Length=296, Percent_Identity=28.0405405405405, Blast_Score=90, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=358, Percent_Identity=23.463687150838, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=31.6455696202532, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=31.6455696202532, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322359, Length=147, Percent_Identity=31.2925170068027, Blast_Score=63, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24652838, Length=444, Percent_Identity=52.2522522522522, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17137572, Length=444, Percent_Identity=52.2522522522522, Blast_Score=483, Evalue=1e-136,
Organism=Drosophila melanogaster, GI45553807, Length=445, Percent_Identity=50.7865168539326, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI45553816, Length=445, Percent_Identity=50.7865168539326, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24651721, Length=445, Percent_Identity=50.7865168539326, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17864154, Length=445, Percent_Identity=50.7865168539326, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI17137380, Length=429, Percent_Identity=36.8298368298368, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI45550900, Length=431, Percent_Identity=34.5707656612529, Blast_Score=290, Evalue=9e-79,
Organism=Drosophila melanogaster, GI281363316, Length=418, Percent_Identity=34.2105263157895, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI17864358, Length=418, Percent_Identity=34.2105263157895, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI19921738, Length=418, Percent_Identity=29.9043062200957, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24647048, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647046, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922690, Length=312, Percent_Identity=23.7179487179487, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922512, Length=379, Percent_Identity=23.7467018469657, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24654852, Length=379, Percent_Identity=23.7467018469657, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI78706572, Length=270, Percent_Identity=25.5555555555556, Blast_Score=72, Evalue=8e-13,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EF1A_METLZ (A2STF0)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030873.1
- ProteinModelPortal:   A2STF0
- SMR:   A2STF0
- STRING:   A2STF0
- GeneID:   4795137
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1441
- eggNOG:   arNOG04484
- HOGENOM:   HBG307581
- OMA:   AFIGHVD
- PhylomeDB:   A2STF0
- ProtClustDB:   PRK12317
- BioCyc:   MLAB410358:MLAB_1441-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004539
- InterPro:   IPR004161
- InterPro:   IPR004160
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00483
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 46505; Mature: 46374

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAAKPHMNLAVIGHIDHGKSTTVGRILFETGVVQQHILDGYKKEAESKGKATFEFAWVM
CCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
DSLKEERERGITIDIAHKKFETPKYNFTVVDCPGHRDFVKNMITGASQADAAIIVVSGTE
HHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCC
GPMEQTKEHVFLSKTLGINQIIVAINKMDAVNYSEEKYNEAKDKMTKLIMSVGFKPAETP
CCHHHHHHHEEEEHHCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
FIPISAFCGDNIKEASANTPWYKGPTLLAALDLFKMPDMPTDKPLRLPIQDVYTISGVGT
EEEEHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHEEEECCCCC
VPVGRVETGILKKGQKISFMPANVTGEVKSIEMHHEEFPEALPGDNVGFNVRGIAKNDVR
CCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHCHHHCCCCCCCEEEEECCCCCCC
RGDVCGPIENPPTVAEEFTAQVVVLQHPSVLSVGYTPVFHCHTSQTACMFTELNKKLDPR
CCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEECCCEEEEECCCCCEEHHHHHHHCCCCC
SGQVKEENPAFLKAGDAAICTITPTRPLVIETAKELPQLGRFAVRDMGMTVAAGLVLSVK
CCCCCCCCCCEEEECCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEE
AKQMR
HHHCC
>Mature Secondary Structure 
AAAKPHMNLAVIGHIDHGKSTTVGRILFETGVVQQHILDGYKKEAESKGKATFEFAWVM
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHH
DSLKEERERGITIDIAHKKFETPKYNFTVVDCPGHRDFVKNMITGASQADAAIIVVSGTE
HHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCC
GPMEQTKEHVFLSKTLGINQIIVAINKMDAVNYSEEKYNEAKDKMTKLIMSVGFKPAETP
CCHHHHHHHEEEEHHCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
FIPISAFCGDNIKEASANTPWYKGPTLLAALDLFKMPDMPTDKPLRLPIQDVYTISGVGT
EEEEHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHEEEECCCCC
VPVGRVETGILKKGQKISFMPANVTGEVKSIEMHHEEFPEALPGDNVGFNVRGIAKNDVR
CCCCCCHHHHHCCCCEEEEEECCCCCCEEEEEHHHHHCHHHCCCCCCCEEEEECCCCCCC
RGDVCGPIENPPTVAEEFTAQVVVLQHPSVLSVGYTPVFHCHTSQTACMFTELNKKLDPR
CCCCCCCCCCCCHHHHHHCEEEEEEECCCEEEECCCEEEEECCCCCEEHHHHHHHCCCCC
SGQVKEENPAFLKAGDAAICTITPTRPLVIETAKELPQLGRFAVRDMGMTVAAGLVLSVK
CCCCCCCCCCEEEECCEEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEE
AKQMR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA