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The map label for this gene is trpB

Identifier: 124486211

GI number: 124486211

Start: 1423785

End: 1424963

Strand: Reverse

Name: trpB

Synonym: Mlab_1394

Alternate gene names: 124486211

Gene position: 1424963-1423785 (Counterclockwise)

Preceding gene: 124486212

Following gene: 124486210

Centisome position: 78.95

GC content: 54.11

Gene sequence:

>1179_bases
ATGAAAGAAAAAACAGGATATTATGGAGAGTTCGGCGGAAGATTCGTCCCCGAAACACTCATGGCGGCTCTCTACGAGCT
GGACAGCGCGTACCACAGATTAAAAGACGACCCGGGGTTCAAGGCTGAACTCAACTCGTATCTGACGGAGTTTGCCGGAC
GCGAGACGCCCCTTACGTTCTGCAGGAATATGTCTGAGTACTGCGGGTGTAAGGTCTATCTCAAGCGCGAGGATCTTGTT
CACGGCGGGGCGCATAAACTCAATAACACCCTTGGGCAGGCTCTTCTCGCAAAAGCAATGGGTAAAAAACGCCTGATCGC
AGAGACCGGGGCCGGACAACACGGCGTTGCAACGGCAATTGCCGGAGCGGCTCTCAATCTGCCGGTCGAAGTGTTCATGG
GCGAGGAAGACTGCGAGAGACAGAAACTGAACGTATTTAGAATGGAACTCATGGGGGCGAAGGTCCATCCTGTCAGTTCC
GGGACGAAGACACTGAAGGATGCAACCAACGAGGCCTTGCGCGAATGGGCAAAGACCGTTGACTACACCCACTATCTGAT
TGGATCGGTTGTGGGTCCCCACCCCTTCCCCATGATCGTCAGGGACTTCCAGTCGGTCATCGGTGAGGAAACAAGACGTC
AGTGTCTTGAGAAAGAAGGAAGACTCCCGGACACGCTTGTTGCCTGCGTCGGCGGTGGCTCGAATGCGATAGGAATGTTT
TACCCGATGCTGAACGACGACGTCCGGATGGTCGGCGTGGAAGCAGGAGGGCGTGCCCTCACTCCCGGAAATAACGGTGC
AACGCTGAACACCGGCAGTCCAGGGATTCTGCACGGGGCTCTTTCCTATCTTATCCAGAATGATGACGGACAGGTCGGCG
TGACGCATTCCATCTCGGCTGGACTCGACTACCCCGGGGTCGGGCCTGAACACAGCATGCTGAAAGATTTGAATCGTGTC
GAGTATTCCTACGTGATGGATGACGAGGTCCTTGAGGCCTTCTCCTATCTTTCACGGACGGAAGGTATCATTCCAGCTCT
CGAATCGGCCCATGCGGTATCGTATGTTCTCAAAAACAGGGATTCTTTCGACCGCGATGATATCGTGGTCATATGCTTAT
CCGGAAGAGGGGATAAGGATGTCTCGTCGGTTTCAAAGATGTTTGGAGGTGAGGAGTAA

Upstream 100 bases:

>100_bases
TGTATGCCGTCGACGTCTCATCCGGCGTTGAAAAATCAAAGGGAATCAAAGACAGAAAAAAGATGCGTGAATTTATAGAA
ATTTGCAGGAGACCAGCAGT

Downstream 100 bases:

>100_bases
TGCCGGCAAAATCCGGAAAAGAGAGGGTTTTTGCAGCGTTTGCAAAACCCGGTTTCGTCGGATACACCGTTGCCGGCGAT
CCAAATATGTCTGATTCGAT

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKEKTGYYGEFGGRFVPETLMAALYELDSAYHRLKDDPGFKAELNSYLTEFAGRETPLTFCRNMSEYCGCKVYLKREDLV
HGGAHKLNNTLGQALLAKAMGKKRLIAETGAGQHGVATAIAGAALNLPVEVFMGEEDCERQKLNVFRMELMGAKVHPVSS
GTKTLKDATNEALREWAKTVDYTHYLIGSVVGPHPFPMIVRDFQSVIGEETRRQCLEKEGRLPDTLVACVGGGSNAIGMF
YPMLNDDVRMVGVEAGGRALTPGNNGATLNTGSPGILHGALSYLIQNDDGQVGVTHSISAGLDYPGVGPEHSMLKDLNRV
EYSYVMDDEVLEAFSYLSRTEGIIPALESAHAVSYVLKNRDSFDRDDIVVICLSGRGDKDVSSVSKMFGGEE

Sequences:

>Translated_392_residues
MKEKTGYYGEFGGRFVPETLMAALYELDSAYHRLKDDPGFKAELNSYLTEFAGRETPLTFCRNMSEYCGCKVYLKREDLV
HGGAHKLNNTLGQALLAKAMGKKRLIAETGAGQHGVATAIAGAALNLPVEVFMGEEDCERQKLNVFRMELMGAKVHPVSS
GTKTLKDATNEALREWAKTVDYTHYLIGSVVGPHPFPMIVRDFQSVIGEETRRQCLEKEGRLPDTLVACVGGGSNAIGMF
YPMLNDDVRMVGVEAGGRALTPGNNGATLNTGSPGILHGALSYLIQNDDGQVGVTHSISAGLDYPGVGPEHSMLKDLNRV
EYSYVMDDEVLEAFSYLSRTEGIIPALESAHAVSYVLKNRDSFDRDDIVVICLSGRGDKDVSSVSKMFGGEE
>Mature_392_residues
MKEKTGYYGEFGGRFVPETLMAALYELDSAYHRLKDDPGFKAELNSYLTEFAGRETPLTFCRNMSEYCGCKVYLKREDLV
HGGAHKLNNTLGQALLAKAMGKKRLIAETGAGQHGVATAIAGAALNLPVEVFMGEEDCERQKLNVFRMELMGAKVHPVSS
GTKTLKDATNEALREWAKTVDYTHYLIGSVVGPHPFPMIVRDFQSVIGEETRRQCLEKEGRLPDTLVACVGGGSNAIGMF
YPMLNDDVRMVGVEAGGRALTPGNNGATLNTGSPGILHGALSYLIQNDDGQVGVTHSISAGLDYPGVGPEHSMLKDLNRV
EYSYVMDDEVLEAFSYLSRTEGIIPALESAHAVSYVLKNRDSFDRDDIVVICLSGRGDKDVSSVSKMFGGEE

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=384, Percent_Identity=54.1666666666667, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321412, Length=384, Percent_Identity=51.5625, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_METLZ (A2STA4)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030827.1
- ProteinModelPortal:   A2STA4
- SMR:   A2STA4
- STRING:   A2STA4
- GeneID:   4795847
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_1394
- eggNOG:   arNOG04577
- HOGENOM:   HBG303148
- OMA:   TFENTHY
- PhylomeDB:   A2STA4
- ProtClustDB:   PRK04346
- BioCyc:   MLAB410358:MLAB_1394-MONOMER
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 42652; Mature: 42652

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKTGYYGEFGGRFVPETLMAALYELDSAYHRLKDDPGFKAELNSYLTEFAGRETPLTF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHH
CRNMSEYCGCKVYLKREDLVHGGAHKLNNTLGQALLAKAMGKKRLIAETGAGQHGVATAI
HHCHHHHCCEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCHHEEHHCCCCCCHHHHHH
AGAALNLPVEVFMGEEDCERQKLNVFRMELMGAKVHPVSSGTKTLKDATNEALREWAKTV
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHH
DYTHYLIGSVVGPHPFPMIVRDFQSVIGEETRRQCLEKEGRLPDTLVACVGGGSNAIGMF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEE
YPMLNDDVRMVGVEAGGRALTPGNNGATLNTGSPGILHGALSYLIQNDDGQVGVTHSISA
ECCCCCCEEEEEEECCCEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCC
GLDYPGVGPEHSMLKDLNRVEYSYVMDDEVLEAFSYLSRTEGIIPALESAHAVSYVLKNR
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
DSFDRDDIVVICLSGRGDKDVSSVSKMFGGEE
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKEKTGYYGEFGGRFVPETLMAALYELDSAYHRLKDDPGFKAELNSYLTEFAGRETPLTF
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHH
CRNMSEYCGCKVYLKREDLVHGGAHKLNNTLGQALLAKAMGKKRLIAETGAGQHGVATAI
HHCHHHHCCEEEEEECHHHHCCCHHHHHHHHHHHHHHHHHCCHHEEHHCCCCCCHHHHHH
AGAALNLPVEVFMGEEDCERQKLNVFRMELMGAKVHPVSSGTKTLKDATNEALREWAKTV
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHCCEECCCCCCHHHHHHHHHHHHHHHHHHH
DYTHYLIGSVVGPHPFPMIVRDFQSVIGEETRRQCLEKEGRLPDTLVACVGGGSNAIGMF
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCEEE
YPMLNDDVRMVGVEAGGRALTPGNNGATLNTGSPGILHGALSYLIQNDDGQVGVTHSISA
ECCCCCCEEEEEEECCCEEECCCCCCCEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCC
GLDYPGVGPEHSMLKDLNRVEYSYVMDDEVLEAFSYLSRTEGIIPALESAHAVSYVLKNR
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
DSFDRDDIVVICLSGRGDKDVSSVSKMFGGEE
CCCCCCCEEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA