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The map label for this gene is trpA

Identifier: 124486210

GI number: 124486210

Start: 1422961

End: 1423785

Strand: Reverse

Name: trpA

Synonym: Mlab_1393

Alternate gene names: 124486210

Gene position: 1423785-1422961 (Counterclockwise)

Preceding gene: 124486211

Following gene: 124486209

Centisome position: 78.88

GC content: 53.09

Gene sequence:

>825_bases
ATGCCGGCAAAATCCGGAAAAGAGAGGGTTTTTGCAGCGTTTGCAAAACCCGGTTTCGTCGGATACACCGTTGCCGGCGA
TCCAAATATGTCTGATTCGATTGAGATCGCGAAAGCCTTGATCGACGGAGGCTGCGATGTTTTGGAATTGGGAGTTCCCT
TTTCCGATCCCGTTGCCGACGGAGGTGTGATCCAGAGCGCTGATAATCGGGCGATTAATGCAGGCATTACAACCGGAGGT
GTATTTGAGATCGTGAAAGCGGTTCGAAGTTACTCGGATGTTCCGATCGTATTTCTTGTCTACTGCAATATCGTGTTCCG
CCGCGGTATCGACCGATTCTATGATGAAGCAAAAGAGGCTGGCGTCGACGGGATTTTGATTGTTGATATGCCGCCGGAAG
AAATCGAGCCTGCCCTGAAAGCTTCAGAGCGGACCGGGCTCGCACAGATTTTCCTAGTGACGCAGACGACCTCGGACGAG
CGGCTTGATATGATCGTTTCGCTCGCTTCCGGCTTCATCTATCTGGTCTCTTCCCTTGGGGTCACCGGAAAGCGTGCTGA
GATTTCGGAAAATGCCTTCCCGCTTCTTGACCGTGTCAAGGCACGGACCGAGACGCCCGTCGCCGTCGGGTTTGGGATCT
CAGAGCCGGCACATGCTGCTGAGATCGTCCGCCATGGTGCGGACGGGGTTATTGTCGGCAGCGCGATTGTTGGAAGGATC
GAGGATCATCTCGGGGATAAAGAAGGAATGCTTGCAGAACTTAAGGCATATGTCCGGAGCATGAAGAATTCTGTTGACGA
TGAAGCGAAGAAACGCGGGATTTGA

Upstream 100 bases:

>100_bases
GGGATTCTTTCGACCGCGATGATATCGTGGTCATATGCTTATCCGGAAGAGGGGATAAGGATGTCTCGTCGGTTTCAAAG
ATGTTTGGAGGTGAGGAGTA

Downstream 100 bases:

>100_bases
GATTCAGTTTACCAAAATCTCAGTTGGAACCCCCACTTATTTTTATTTTTGTATGACCAACAGAATCATACGTGTCTGAT
GTCATCGTCATAGGAGGGGG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPAKSGKERVFAAFAKPGFVGYTVAGDPNMSDSIEIAKALIDGGCDVLELGVPFSDPVADGGVIQSADNRAINAGITTGG
VFEIVKAVRSYSDVPIVFLVYCNIVFRRGIDRFYDEAKEAGVDGILIVDMPPEEIEPALKASERTGLAQIFLVTQTTSDE
RLDMIVSLASGFIYLVSSLGVTGKRAEISENAFPLLDRVKARTETPVAVGFGISEPAHAAEIVRHGADGVIVGSAIVGRI
EDHLGDKEGMLAELKAYVRSMKNSVDDEAKKRGI

Sequences:

>Translated_274_residues
MPAKSGKERVFAAFAKPGFVGYTVAGDPNMSDSIEIAKALIDGGCDVLELGVPFSDPVADGGVIQSADNRAINAGITTGG
VFEIVKAVRSYSDVPIVFLVYCNIVFRRGIDRFYDEAKEAGVDGILIVDMPPEEIEPALKASERTGLAQIFLVTQTTSDE
RLDMIVSLASGFIYLVSSLGVTGKRAEISENAFPLLDRVKARTETPVAVGFGISEPAHAAEIVRHGADGVIVGSAIVGRI
EDHLGDKEGMLAELKAYVRSMKNSVDDEAKKRGI
>Mature_273_residues
PAKSGKERVFAAFAKPGFVGYTVAGDPNMSDSIEIAKALIDGGCDVLELGVPFSDPVADGGVIQSADNRAINAGITTGGV
FEIVKAVRSYSDVPIVFLVYCNIVFRRGIDRFYDEAKEAGVDGILIVDMPPEEIEPALKASERTGLAQIFLVTQTTSDER
LDMIVSLASGFIYLVSSLGVTGKRAEISENAFPLLDRVKARTETPVAVGFGISEPAHAAEIVRHGADGVIVGSAIVGRIE
DHLGDKEGMLAELKAYVRSMKNSVDDEAKKRGI

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=248, Percent_Identity=42.3387096774194, Blast_Score=183, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321412, Length=247, Percent_Identity=38.8663967611336, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 29134; Mature: 29002

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAKSGKERVFAAFAKPGFVGYTVAGDPNMSDSIEIAKALIDGGCDVLELGVPFSDPVAD
CCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GGVIQSADNRAINAGITTGGVFEIVKAVRSYSDVPIVFLVYCNIVFRRGIDRFYDEAKEA
CCEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GVDGILIVDMPPEEIEPALKASERTGLAQIFLVTQTTSDERLDMIVSLASGFIYLVSSLG
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
VTGKRAEISENAFPLLDRVKARTETPVAVGFGISEPAHAAEIVRHGADGVIVGSAIVGRI
CCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHHH
EDHLGDKEGMLAELKAYVRSMKNSVDDEAKKRGI
HHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure 
PAKSGKERVFAAFAKPGFVGYTVAGDPNMSDSIEIAKALIDGGCDVLELGVPFSDPVAD
CCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GGVIQSADNRAINAGITTGGVFEIVKAVRSYSDVPIVFLVYCNIVFRRGIDRFYDEAKEA
CCEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GVDGILIVDMPPEEIEPALKASERTGLAQIFLVTQTTSDERLDMIVSLASGFIYLVSSLG
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCC
VTGKRAEISENAFPLLDRVKARTETPVAVGFGISEPAHAAEIVRHGADGVIVGSAIVGRI
CCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCEEEHHHHHHHH
EDHLGDKEGMLAELKAYVRSMKNSVDDEAKKRGI
HHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA