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The map label for this gene is rpoB2 [H]

Identifier: 124485983

GI number: 124485983

Start: 1183909

End: 1185459

Strand: Reverse

Name: rpoB2 [H]

Synonym: Mlab_1163

Alternate gene names: 124485983

Gene position: 1185459-1183909 (Counterclockwise)

Preceding gene: 124485984

Following gene: 124485982

Centisome position: 65.68

GC content: 54.67

Gene sequence:

>1551_bases
ATGATCGACAGAAGTGTATTATCAGGCGCGTACTTTTCCCAGGAACACGTTGCACGCCACCAGCTGGACTCCTATAACAA
TTTTGTCCAGTTTAACCTCCAGAAAGTCGTAGACGAACAGCGTATCGTCGAGACCGAAATCGTCAACAGAGGAAAGAACC
AGGAGGCCGTGTGGGTCGAACTGGGAAAAATCCGTGTTGAAAAACCCATCGTCAGGGAAGCAGACGGTTCGCAGAGCAAG
CTCTATCCGGCCGAAGCCCGCCTAAGAAACCTTTCCTATGCGGCCCCGATGATCCTTGAAATGACCCTCCACCAGGGAGA
CAAAGTCTATCCGGTCCAGGAAACGACCATCGGTCAGCTCCCGGTCATGATCGGCAGTCAGGTCTGTAACCTCTGCGGTC
TGTCCCAGGAAGAGAGAACCGAGCAGGGCGAGGATCCGAGCGATCCCGGAGGATACTTCATCGTCAACGGAACCGAGCGT
GTATTAATGACGCTCGAAGATCTTGCCAGCAACAAGATCATGACCGAGTTCACCGAGAGATACAACGAGCAGATCCATGT
ATCCAAAGTGTTCTCCCAGTTCCGCGGCTACCGCGCTCTCGTCGTTGTCGAGAAGAACAAGAAGAACCTCCTCGACGTCT
CCTTCCCGAGCGTTGCCGGCCACCTGCGGTTTGCGGACCTCATGCGTGCGCTTGGTCTCGAGTCCGATGAAGAGATCGTA
TCGGCCGTCTCCCTCTCAGAAGACGTCCTCACCTACATGATGCAGAACCTCGAAGAAAGCGAATGCGCCACCGTCGAGGA
GGGTGTCATGTATGTCGGTAAGAAGCTTGCGCCCAACCAGACCAAGGATTACCAGAGAAAACGCGCCGAGTTCGTTTTGG
ACAGCTACCTTCTCCCGCACTTAAACTACGCCATCCCCGGCTCCTTAAAACCCGGAGACGAAGGCTACGAAGTGTATGCA
AAGGATGTCCGGATCGCCAAAGCCGAATTCCTCGGCAGAATGGCAGAGGCATGTTTCGACCTCGCACTCAACAAGAGGAG
AATCGACGACAAAGATCACTACTCCAACAAGCGCCTGAAACTTGCAGGTGACCTGATGGAGGATCTGTTCCGTATCTCGT
TAAACAGACTTACCCGCGATGTCAAGTATCAGCTTGAACGCGCGAGCATGAGACACAGAGAACTCGCGATCGGAACCGTC
GTCCGTGCGGATGTCTTGACCGAACGCCTGATTCACCCGCTTGCAACCGGAAACTGGGTCGGCGGAAGGACTGGTGTCTC
CCAGCTTATGGACCGCGTCGACCACATGTCGGTCATTTCCCACCTGCGAAGAGTTATCTCTCCGCTGTCCAGATCCCAGC
CCCACTTCGAGGCCCGTGATCTGCACCCAACCCAGTGGGGGCGCATCTGTCCGTCCGAAACTCCGGAAGGACCAAACTGC
GGTTTAGTGAAGAACTTTGCCCAGCTTGTCGAGATCAGTAAAGGAACCGATGAGGACGAGGTCCTCCGTGTAATACAGGG
CATGAAGATCCAGCCGATCAGGAGCGAATAA

Upstream 100 bases:

>100_bases
AAACGTCATTATGATCGTAAGAAAGAGCCAGACAGCAGGCGAAGCCACATCCTACCGCCTTGTCGTTAGAAGGCCGAAAA
AATAACCGGAGGCAGAAATC

Downstream 100 bases:

>100_bases
AATGGAAAAGAAAATGGCACGTGTATTCGTGGACGGTGCTTTGATTGGCAAAGTCGACGATGCCTACGGCTTCACCAGAA
ACTTCAGACAGATGCGCAGA

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MIDRSVLSGAYFSQEHVARHQLDSYNNFVQFNLQKVVDEQRIVETEIVNRGKNQEAVWVELGKIRVEKPIVREADGSQSK
LYPAEARLRNLSYAAPMILEMTLHQGDKVYPVQETTIGQLPVMIGSQVCNLCGLSQEERTEQGEDPSDPGGYFIVNGTER
VLMTLEDLASNKIMTEFTERYNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSVAGHLRFADLMRALGLESDEEIV
SAVSLSEDVLTYMMQNLEESECATVEEGVMYVGKKLAPNQTKDYQRKRAEFVLDSYLLPHLNYAIPGSLKPGDEGYEVYA
KDVRIAKAEFLGRMAEACFDLALNKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRHRELAIGTV
VRADVLTERLIHPLATGNWVGGRTGVSQLMDRVDHMSVISHLRRVISPLSRSQPHFEARDLHPTQWGRICPSETPEGPNC
GLVKNFAQLVEISKGTDEDEVLRVIQGMKIQPIRSE

Sequences:

>Translated_516_residues
MIDRSVLSGAYFSQEHVARHQLDSYNNFVQFNLQKVVDEQRIVETEIVNRGKNQEAVWVELGKIRVEKPIVREADGSQSK
LYPAEARLRNLSYAAPMILEMTLHQGDKVYPVQETTIGQLPVMIGSQVCNLCGLSQEERTEQGEDPSDPGGYFIVNGTER
VLMTLEDLASNKIMTEFTERYNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSVAGHLRFADLMRALGLESDEEIV
SAVSLSEDVLTYMMQNLEESECATVEEGVMYVGKKLAPNQTKDYQRKRAEFVLDSYLLPHLNYAIPGSLKPGDEGYEVYA
KDVRIAKAEFLGRMAEACFDLALNKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRHRELAIGTV
VRADVLTERLIHPLATGNWVGGRTGVSQLMDRVDHMSVISHLRRVISPLSRSQPHFEARDLHPTQWGRICPSETPEGPNC
GLVKNFAQLVEISKGTDEDEVLRVIQGMKIQPIRSE
>Mature_516_residues
MIDRSVLSGAYFSQEHVARHQLDSYNNFVQFNLQKVVDEQRIVETEIVNRGKNQEAVWVELGKIRVEKPIVREADGSQSK
LYPAEARLRNLSYAAPMILEMTLHQGDKVYPVQETTIGQLPVMIGSQVCNLCGLSQEERTEQGEDPSDPGGYFIVNGTER
VLMTLEDLASNKIMTEFTERYNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSVAGHLRFADLMRALGLESDEEIV
SAVSLSEDVLTYMMQNLEESECATVEEGVMYVGKKLAPNQTKDYQRKRAEFVLDSYLLPHLNYAIPGSLKPGDEGYEVYA
KDVRIAKAEFLGRMAEACFDLALNKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRHRELAIGTV
VRADVLTERLIHPLATGNWVGGRTGVSQLMDRVDHMSVISHLRRVISPLSRSQPHFEARDLHPTQWGRICPSETPEGPNC
GLVKNFAQLVEISKGTDEDEVLRVIQGMKIQPIRSE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=540, Percent_Identity=30.7407407407407, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI238908503, Length=527, Percent_Identity=26.7552182163188, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI238908505, Length=472, Percent_Identity=28.1779661016949, Blast_Score=193, Evalue=4e-49,
Organism=Escherichia coli, GI1790419, Length=154, Percent_Identity=37.012987012987, Blast_Score=101, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17552304, Length=542, Percent_Identity=30.4428044280443, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI25144348, Length=528, Percent_Identity=29.5454545454545, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17506623, Length=519, Percent_Identity=24.2774566473988, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324725, Length=563, Percent_Identity=30.550621669627, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324781, Length=538, Percent_Identity=27.5092936802974, Blast_Score=196, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6325267, Length=514, Percent_Identity=23.9299610894942, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136444, Length=536, Percent_Identity=32.2761194029851, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI17647877, Length=531, Percent_Identity=26.5536723163842, Blast_Score=198, Evalue=7e-51,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 58588; Mature: 58588

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRSVLSGAYFSQEHVARHQLDSYNNFVQFNLQKVVDEQRIVETEIVNRGKNQEAVWVE
CCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LGKIRVEKPIVREADGSQSKLYPAEARLRNLSYAAPMILEMTLHQGDKVYPVQETTIGQL
ECCEEECCCHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCEEECCCCCCCCCC
PVMIGSQVCNLCGLSQEERTEQGEDPSDPGGYFIVNGTERVLMTLEDLASNKIMTEFTER
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHH
YNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSVAGHLRFADLMRALGLESDEEIV
HCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHH
SAVSLSEDVLTYMMQNLEESECATVEEGVMYVGKKLAPNQTKDYQRKRAEFVLDSYLLPH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNYAIPGSLKPGDEGYEVYAKDVRIAKAEFLGRMAEACFDLALNKRRIDDKDHYSNKRLK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHH
LAGDLMEDLFRISLNRLTRDVKYQLERASMRHRELAIGTVVRADVLTERLIHPLATGNWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGRTGVSQLMDRVDHMSVISHLRRVISPLSRSQPHFEARDLHPTQWGRICPSETPEGPNC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLVKNFAQLVEISKGTDEDEVLRVIQGMKIQPIRSE
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIDRSVLSGAYFSQEHVARHQLDSYNNFVQFNLQKVVDEQRIVETEIVNRGKNQEAVWVE
CCCHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LGKIRVEKPIVREADGSQSKLYPAEARLRNLSYAAPMILEMTLHQGDKVYPVQETTIGQL
ECCEEECCCHHHCCCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCEEECCCCCCCCCC
PVMIGSQVCNLCGLSQEERTEQGEDPSDPGGYFIVNGTERVLMTLEDLASNKIMTEFTER
HHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHH
YNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSVAGHLRFADLMRALGLESDEEIV
HCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHCCCCHHHHH
SAVSLSEDVLTYMMQNLEESECATVEEGVMYVGKKLAPNQTKDYQRKRAEFVLDSYLLPH
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNYAIPGSLKPGDEGYEVYAKDVRIAKAEFLGRMAEACFDLALNKRRIDDKDHYSNKRLK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHH
LAGDLMEDLFRISLNRLTRDVKYQLERASMRHRELAIGTVVRADVLTERLIHPLATGNWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GGRTGVSQLMDRVDHMSVISHLRRVISPLSRSQPHFEARDLHPTQWGRICPSETPEGPNC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLVKNFAQLVEISKGTDEDEVLRVIQGMKIQPIRSE
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2495365; 11016950 [H]