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The map label for this gene is rpoB1 [H]

Identifier: 124485982

GI number: 124485982

Start: 1181994

End: 1183907

Strand: Reverse

Name: rpoB1 [H]

Synonym: Mlab_1162

Alternate gene names: 124485982

Gene position: 1183907-1181994 (Counterclockwise)

Preceding gene: 124485983

Following gene: 124485981

Centisome position: 65.59

GC content: 54.39

Gene sequence:

>1914_bases
ATGGAAAAGAAAATGGCACGTGTATTCGTGGACGGTGCTTTGATTGGCAAAGTCGACGATGCCTACGGCTTCACCAGAAA
CTTCAGACAGATGCGCAGATCGGGGCACGTCTCGACCGAAGTCAATATTTCATACAAAGATTACAGTAACGAGATCGTCA
TCAATACCGACCGCGGACGGGCCCGCAGACCCCTGATCGTCGTCGAAAACGGCGTCCCGGCAGTCACCCCCGAAGATATC
GAGATCGTCAGATCCGGTGCTGAAGACTTTATGGCACTGGTATCTCAGGGTAAGATCGAGTTCATCGATGCGGAAGAGGA
GGATGACCTCTTTATCGCGGTGCAGGAATCCGATCTGACTCCAGAACACACCCACCTTGAGATCGATCCGTCCCTTATCC
TCGGTATAGGAGCAGCACACGTTCCGTTCCCGGAACACAACGCATCGCCGCGTGTTACGATGGGTGCGGGTATGATCAAA
CAGGCACTGGGTTTTGCCCAGGCAAACATGAAGCTTAGGCCCGATACCCGCGGTCATATGCTTCACTACGCCCAGGTCCC
GATGGTGCACACGCAGGCAGCCGAACTTATCGGTTCCGACAACCGCCCGCAGGGTCAGAACTTCTGTGTGGCCATCATCT
CCTACGAAGGATACAATATTGAAGATGCACTCATCTTCAACAAAGCCTCGGTCGAACGCGGAGTAGGCCGTTCCCACTTC
TTCAGAACCTATGAAGGAGAGGAACGCAGATATCCCGGAGGACAGGTCGACAGGATCGAGCTGCCCGAAGAGGATGTCCA
GGGTTCCCATGGAATCGGTTCCTACGCAAACCTCGATGTTGATGGAATCATCAACCCCGAGACCGTCGTCAATGAAAAGG
ATGTTCTCATCGGCAAGACCTCTCCGCCGCGTTTCCTCGAGGAACCGACCGGAGAACTCATCGCCGTTGAAAAACGCCGC
GACACGTCCATCACGATGAGAAGTAACGAGACCGGTATTGTAGACACCGTCATCATCACCGAGTCCGAGAACTCCTCCCG
CCTCGTCAAAGTCAGAACACGTGATCTCCGTATCCCGGAGATCGGCGACAAGTTCGCATCCCGTCACGGTCAGAAAGGTG
TCTGCGGTCTTATCACTCCGCAGGAAAACATGCCTTTCACCGCTGCAGGTATCGCGCCCGATCTCGTCATCAACCCGCAC
GCAGTCCCGTCCCGTATGACCATCGGTCATATGCTCGAGATGATCGGCGGCAAAGCCGGAGCACTCGAAGGTTCCCGCGT
GAATGCAACTTCCTTCCGAAAGACCCAGGTCGAGAAGATCTTCGACTCGTCCTTCGCACAGAACCGTCTCTTAAACGACA
AAGAGATCGGCAAAGACGTGAAGTCGAGCACCCTCACCCGTGAGCAGGTCTTACGACACCAGCTTGCCGAGGCAGGATTC
GCCCACACCGGCCGTGAAGTCATGTACGACGGTATCACCGGCCGCAGATTCCAGGCCGACATTTACATCGGTGTTATCTA
CTACCAGAAACTCTACCACATGGTATCTACCAAGATGCATGCACGGTCCAGAGGTCCGGTCCAGGTCCTTACCCGTCAGC
CGACCGAAGGACGTGCCCGTGAGGGAGGTCTTCGTTTCGGTGAAATGGAACGTGATGTGATGATCGGTCACGGTGCCGCA
ATGGCTCTCAAAGAGCGTCTCCTTGACGAATCCGATGCAGTAAAGCAGTACGTCTGCGCACGCTGCGGAATGGTCGCCAT
GTATGATGCCAAGCAGAAGATGACCCGCTGTCTCGCCTGCGGCGCAGAAACAGATATTTATGAAGTCGAAATGAGTTACG
CATTCAAACTTCTCCTTGATGAGATGAAGAGTATGGGTATAGCTCCCAGACTGAGACTGGAGGATATGGTATGA

Upstream 100 bases:

>100_bases
AACTTTGCCCAGCTTGTCGAGATCAGTAAAGGAACCGATGAGGACGAGGTCCTCCGTGTAATACAGGGCATGAAGATCCA
GCCGATCAGGAGCGAATAAA

Downstream 100 bases:

>100_bases
CCTCGCCAAAGAGAATCGGCAAGATCGAGTTCGGTATCCTTTCCCCGAAAGCGATCCGCGACATGAGTGTCTGTAAGGTC
ATCTGGCCGGACACCTACGA

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MEKKMARVFVDGALIGKVDDAYGFTRNFRQMRRSGHVSTEVNISYKDYSNEIVINTDRGRARRPLIVVENGVPAVTPEDI
EIVRSGAEDFMALVSQGKIEFIDAEEEDDLFIAVQESDLTPEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIK
QALGFAQANMKLRPDTRGHMLHYAQVPMVHTQAAELIGSDNRPQGQNFCVAIISYEGYNIEDALIFNKASVERGVGRSHF
FRTYEGEERRYPGGQVDRIELPEEDVQGSHGIGSYANLDVDGIINPETVVNEKDVLIGKTSPPRFLEEPTGELIAVEKRR
DTSITMRSNETGIVDTVIITESENSSRLVKVRTRDLRIPEIGDKFASRHGQKGVCGLITPQENMPFTAAGIAPDLVINPH
AVPSRMTIGHMLEMIGGKAGALEGSRVNATSFRKTQVEKIFDSSFAQNRLLNDKEIGKDVKSSTLTREQVLRHQLAEAGF
AHTGREVMYDGITGRRFQADIYIGVIYYQKLYHMVSTKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVMIGHGAA
MALKERLLDESDAVKQYVCARCGMVAMYDAKQKMTRCLACGAETDIYEVEMSYAFKLLLDEMKSMGIAPRLRLEDMV

Sequences:

>Translated_637_residues
MEKKMARVFVDGALIGKVDDAYGFTRNFRQMRRSGHVSTEVNISYKDYSNEIVINTDRGRARRPLIVVENGVPAVTPEDI
EIVRSGAEDFMALVSQGKIEFIDAEEEDDLFIAVQESDLTPEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIK
QALGFAQANMKLRPDTRGHMLHYAQVPMVHTQAAELIGSDNRPQGQNFCVAIISYEGYNIEDALIFNKASVERGVGRSHF
FRTYEGEERRYPGGQVDRIELPEEDVQGSHGIGSYANLDVDGIINPETVVNEKDVLIGKTSPPRFLEEPTGELIAVEKRR
DTSITMRSNETGIVDTVIITESENSSRLVKVRTRDLRIPEIGDKFASRHGQKGVCGLITPQENMPFTAAGIAPDLVINPH
AVPSRMTIGHMLEMIGGKAGALEGSRVNATSFRKTQVEKIFDSSFAQNRLLNDKEIGKDVKSSTLTREQVLRHQLAEAGF
AHTGREVMYDGITGRRFQADIYIGVIYYQKLYHMVSTKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVMIGHGAA
MALKERLLDESDAVKQYVCARCGMVAMYDAKQKMTRCLACGAETDIYEVEMSYAFKLLLDEMKSMGIAPRLRLEDMV
>Mature_637_residues
MEKKMARVFVDGALIGKVDDAYGFTRNFRQMRRSGHVSTEVNISYKDYSNEIVINTDRGRARRPLIVVENGVPAVTPEDI
EIVRSGAEDFMALVSQGKIEFIDAEEEDDLFIAVQESDLTPEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIK
QALGFAQANMKLRPDTRGHMLHYAQVPMVHTQAAELIGSDNRPQGQNFCVAIISYEGYNIEDALIFNKASVERGVGRSHF
FRTYEGEERRYPGGQVDRIELPEEDVQGSHGIGSYANLDVDGIINPETVVNEKDVLIGKTSPPRFLEEPTGELIAVEKRR
DTSITMRSNETGIVDTVIITESENSSRLVKVRTRDLRIPEIGDKFASRHGQKGVCGLITPQENMPFTAAGIAPDLVINPH
AVPSRMTIGHMLEMIGGKAGALEGSRVNATSFRKTQVEKIFDSSFAQNRLLNDKEIGKDVKSSTLTREQVLRHQLAEAGF
AHTGREVMYDGITGRRFQADIYIGVIYYQKLYHMVSTKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVMIGHGAA
MALKERLLDESDAVKQYVCARCGMVAMYDAKQKMTRCLACGAETDIYEVEMSYAFKLLLDEMKSMGIAPRLRLEDMV

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The B (B''+B' and beta) subunits have been implicated in DNA promoter recognition and also in nucleotide binding [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4505941, Length=642, Percent_Identity=41.7445482866044, Blast_Score=481, Evalue=1e-136,
Organism=Homo sapiens, GI238908505, Length=627, Percent_Identity=40.9888357256778, Blast_Score=481, Evalue=1e-135,
Organism=Homo sapiens, GI238908503, Length=627, Percent_Identity=40.9888357256778, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI33469941, Length=554, Percent_Identity=32.6714801444043, Blast_Score=285, Evalue=1e-76,
Organism=Homo sapiens, GI212286172, Length=554, Percent_Identity=32.6714801444043, Blast_Score=285, Evalue=1e-76,
Organism=Escherichia coli, GI1790419, Length=314, Percent_Identity=34.0764331210191, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17552304, Length=641, Percent_Identity=41.6536661466459, Blast_Score=480, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144348, Length=635, Percent_Identity=39.3700787401575, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17506623, Length=562, Percent_Identity=31.3167259786477, Blast_Score=276, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6324725, Length=670, Percent_Identity=41.044776119403, Blast_Score=504, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6324781, Length=630, Percent_Identity=41.5873015873016, Blast_Score=475, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6325267, Length=475, Percent_Identity=37.4736842105263, Blast_Score=297, Evalue=4e-81,
Organism=Drosophila melanogaster, GI17136444, Length=642, Percent_Identity=42.0560747663551, Blast_Score=489, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17647877, Length=628, Percent_Identity=40.7643312101911, Blast_Score=475, Evalue=1e-134,
Organism=Drosophila melanogaster, GI17136446, Length=553, Percent_Identity=31.4647377938517, Blast_Score=275, Evalue=5e-74,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 70899; Mature: 70899

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKMARVFVDGALIGKVDDAYGFTRNFRQMRRSGHVSTEVNISYKDYSNEIVINTDRGR
CCCHHEEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCC
ARRPLIVVENGVPAVTPEDIEIVRSGAEDFMALVSQGKIEFIDAEEEDDLFIAVQESDLT
CCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCC
PEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIKQALGFAQANMKLRPDTRGHM
CCCCEEEECCCEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCEECCCCCCCE
LHYAQVPMVHTQAAELIGSDNRPQGQNFCVAIISYEGYNIEDALIFNKASVERGVGRSHF
EEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCCHHHE
FRTYEGEERRYPGGQVDRIELPEEDVQGSHGIGSYANLDVDGIINPETVVNEKDVLIGKT
EECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECC
SPPRFLEEPTGELIAVEKRRDTSITMRSNETGIVDTVIITESENSSRLVKVRTRDLRIPE
CCCHHHHCCCCCEEEEEECCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEEECCCCCCC
IGDKFASRHGQKGVCGLITPQENMPFTAAGIAPDLVINPHAVPSRMTIGHMLEMIGGKAG
CCHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCC
ALEGSRVNATSFRKTQVEKIFDSSFAQNRLLNDKEIGKDVKSSTLTREQVLRHQLAEAGF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCC
AHTGREVMYDGITGRRFQADIYIGVIYYQKLYHMVSTKMHARSRGPVQVLTRQPTEGRAR
CCCCCHHEECCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
EGGLRFGEMERDVMIGHGAAMALKERLLDESDAVKQYVCARCGMVAMYDAKQKMTRCLAC
HCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHC
GAETDIYEVEMSYAFKLLLDEMKSMGIAPRLRLEDMV
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MEKKMARVFVDGALIGKVDDAYGFTRNFRQMRRSGHVSTEVNISYKDYSNEIVINTDRGR
CCCHHEEEEECCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEECCCCC
ARRPLIVVENGVPAVTPEDIEIVRSGAEDFMALVSQGKIEFIDAEEEDDLFIAVQESDLT
CCCCEEEEECCCCCCCCCHHHHHHCCHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCC
PEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIKQALGFAQANMKLRPDTRGHM
CCCCEEEECCCEEEECCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCEECCCCCCCE
LHYAQVPMVHTQAAELIGSDNRPQGQNFCVAIISYEGYNIEDALIFNKASVERGVGRSHF
EEEECCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCEEEEEHHHHHHCCCHHHE
FRTYEGEERRYPGGQVDRIELPEEDVQGSHGIGSYANLDVDGIINPETVVNEKDVLIGKT
EECCCCCCCCCCCCCEEEEECCHHHCCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEEECC
SPPRFLEEPTGELIAVEKRRDTSITMRSNETGIVDTVIITESENSSRLVKVRTRDLRIPE
CCCHHHHCCCCCEEEEEECCCCEEEEECCCCCEEEEEEEEECCCCCEEEEEEECCCCCCC
IGDKFASRHGQKGVCGLITPQENMPFTAAGIAPDLVINPHAVPSRMTIGHMLEMIGGKAG
CCHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCEEECCCCCCCCHHHHHHHHHHCCCCC
ALEGSRVNATSFRKTQVEKIFDSSFAQNRLLNDKEIGKDVKSSTLTREQVLRHQLAEAGF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCC
AHTGREVMYDGITGRRFQADIYIGVIYYQKLYHMVSTKMHARSRGPVQVLTRQPTEGRAR
CCCCCHHEECCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCH
EGGLRFGEMERDVMIGHGAAMALKERLLDESDAVKQYVCARCGMVAMYDAKQKMTRCLAC
HCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHC
GAETDIYEVEMSYAFKLLLDEMKSMGIAPRLRLEDMV
CCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]