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The map label for this gene is nifE [H]

Identifier: 124485859

GI number: 124485859

Start: 1049426

End: 1050589

Strand: Reverse

Name: nifE [H]

Synonym: Mlab_1039

Alternate gene names: 124485859

Gene position: 1050589-1049426 (Counterclockwise)

Preceding gene: 124485860

Following gene: 124485856

Centisome position: 58.21

GC content: 42.27

Gene sequence:

>1164_bases
ATGAGCAATCTGTTTGTGCACCTGCCGTCATTTGCAACGGATTACTCCGGCGCCGCTTCGGTATTTTATAATATGGGCGG
ACTTGTTGTGATCCATGAACCATCAGGATGTATGGGTAATTTTACCGGATTTGACGAACCCCGCTGGTACCATGCTCCAG
AGATGGTATTTAGTTCATTTATCCGTGAGGAAGAGGCAACGATGGGTGATGAGTCGATTCTTCTCAATAAAATTCTTCAG
GAATGCGCAAGTCATCATCCCAAATTTGTTGCGATTCTTGGAACACCAGTCCCTGCATTAATCGGTTGCGATATCTCGGG
AATTGCAACTGAGGTTTTTGATACTACCAAAATCCCCGCATTTGGGCTGAATACTACTGGATTTCAGTATTATGATGATG
GTATCAAAAAAGCTCTCCTGATGATCGAAGAGCAGTTAATGAAGGACTCAGAAGTAAAAGAGCCAAAAACAGTAAATATC
CTTGGATACACGCCCATAGATTTTTTCCTTTCAGGTGACGATAGGAGGTTAGCATCATTTGTAGAGTCGTGCGGATTTCG
GATTTTATGTTCTCTCCCCGGAGATGAGCTGGAGACGATTATCAAAGCACCCAATGCAGAAAAAAATATTGTGGTGTCCG
CGGCAGCAATTCCTCTTGCAGAAAAAATGAGGGAAAAATATGGCATTCCATTCTGCGCAATGCTTCCGGGAACGACGAAC
GGCAAAGAGAGAATGCAGGCATTTTTACAGGATTATACGCCGCAACTTCCCGCATCTTGCGTTTGTTCCGGAAAAGCTCT
TGTAATTGGGGAACAGATTTCTGCGAACACTATACGTGATTTCCTGATTGAAGACCTGAATTATTCTAACGTGAATGTCG
CCACGTTCTTTGCATTTTCTCGTACTATCGCACGTGAAGGAGACATCAAACTTTCAAATGAAGGACATCTCCAAAAACTT
ATCGAAGAAGGGTGTTACGATATCATTGTTGGTGATCCGCTGTTTGAACGGTTTACGAACCGGCAACAGATTTTTGTTTC
ACTTCCACATCCGGCAGTAAGCAGTAAATTACATTGGCAATCGTATGTTTCTCTTCTCAGCAACGATTTTTCAAAATATC
TTTATGAAAAAATCAATCGCAAATATCCTGTAACATTACAGTAG

Upstream 100 bases:

>100_bases
CTACTATGGGATAGAACTATTGATGAATCTTTTAATTGAGAGTGTATCTCATCCAAAGGATCTGCGCAAAATGATCGAAG
CATATGGACTGGTGGCATAA

Downstream 100 bases:

>100_bases
AGCAGAGAATGATAATATTAGATCGAGTGATGCGCTGAAAATAAATCTTCAAAATATCACAGCGCATTACACAACATTTT
TCAAAAAAAATATGCCTGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSNLFVHLPSFATDYSGAASVFYNMGGLVVIHEPSGCMGNFTGFDEPRWYHAPEMVFSSFIREEEATMGDESILLNKILQ
ECASHHPKFVAILGTPVPALIGCDISGIATEVFDTTKIPAFGLNTTGFQYYDDGIKKALLMIEEQLMKDSEVKEPKTVNI
LGYTPIDFFLSGDDRRLASFVESCGFRILCSLPGDELETIIKAPNAEKNIVVSAAAIPLAEKMREKYGIPFCAMLPGTTN
GKERMQAFLQDYTPQLPASCVCSGKALVIGEQISANTIRDFLIEDLNYSNVNVATFFAFSRTIAREGDIKLSNEGHLQKL
IEEGCYDIIVGDPLFERFTNRQQIFVSLPHPAVSSKLHWQSYVSLLSNDFSKYLYEKINRKYPVTLQ

Sequences:

>Translated_387_residues
MSNLFVHLPSFATDYSGAASVFYNMGGLVVIHEPSGCMGNFTGFDEPRWYHAPEMVFSSFIREEEATMGDESILLNKILQ
ECASHHPKFVAILGTPVPALIGCDISGIATEVFDTTKIPAFGLNTTGFQYYDDGIKKALLMIEEQLMKDSEVKEPKTVNI
LGYTPIDFFLSGDDRRLASFVESCGFRILCSLPGDELETIIKAPNAEKNIVVSAAAIPLAEKMREKYGIPFCAMLPGTTN
GKERMQAFLQDYTPQLPASCVCSGKALVIGEQISANTIRDFLIEDLNYSNVNVATFFAFSRTIAREGDIKLSNEGHLQKL
IEEGCYDIIVGDPLFERFTNRQQIFVSLPHPAVSSKLHWQSYVSLLSNDFSKYLYEKINRKYPVTLQ
>Mature_386_residues
SNLFVHLPSFATDYSGAASVFYNMGGLVVIHEPSGCMGNFTGFDEPRWYHAPEMVFSSFIREEEATMGDESILLNKILQE
CASHHPKFVAILGTPVPALIGCDISGIATEVFDTTKIPAFGLNTTGFQYYDDGIKKALLMIEEQLMKDSEVKEPKTVNIL
GYTPIDFFLSGDDRRLASFVESCGFRILCSLPGDELETIIKAPNAEKNIVVSAAAIPLAEKMREKYGIPFCAMLPGTTNG
KERMQAFLQDYTPQLPASCVCSGKALVIGEQISANTIRDFLIEDLNYSNVNVATFFAFSRTIAREGDIKLSNEGHLQKLI
EEGCYDIIVGDPLFERFTNRQQIFVSLPHPAVSSKLHWQSYVSLLSNDFSKYLYEKINRKYPVTLQ

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 43091; Mature: 42960

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLFVHLPSFATDYSGAASVFYNMGGLVVIHEPSGCMGNFTGFDEPRWYHAPEMVFSSF
CCCEEEECCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
IREEEATMGDESILLNKILQECASHHPKFVAILGTPVPALIGCDISGIATEVFDTTKIPA
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCC
FGLNTTGFQYYDDGIKKALLMIEEQLMKDSEVKEPKTVNILGYTPIDFFLSGDDRRLASF
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEECCCCHHHHHH
VESCGFRILCSLPGDELETIIKAPNAEKNIVVSAAAIPLAEKMREKYGIPFCAMLPGTTN
HHHCCCEEEECCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCCC
GKERMQAFLQDYTPQLPASCVCSGKALVIGEQISANTIRDFLIEDLNYSNVNVATFFAFS
HHHHHHHHHHHCCCCCCHHHEECCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RTIAREGDIKLSNEGHLQKLIEEGCYDIIVGDPLFERFTNRQQIFVSLPHPAVSSKLHWQ
HHHHCCCCEEECCCHHHHHHHHCCCEEEEECCHHHHHHCCCEEEEEECCCCHHCCCHHHH
SYVSLLSNDFSKYLYEKINRKYPVTLQ
HHHHHHHHHHHHHHHHHHCCCCCCEEC
>Mature Secondary Structure 
SNLFVHLPSFATDYSGAASVFYNMGGLVVIHEPSGCMGNFTGFDEPRWYHAPEMVFSSF
CCEEEECCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHH
IREEEATMGDESILLNKILQECASHHPKFVAILGTPVPALIGCDISGIATEVFDTTKIPA
HHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCHHHHHHHHHCCCCCC
FGLNTTGFQYYDDGIKKALLMIEEQLMKDSEVKEPKTVNILGYTPIDFFLSGDDRRLASF
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCEEEEECCCCHHHHHH
VESCGFRILCSLPGDELETIIKAPNAEKNIVVSAAAIPLAEKMREKYGIPFCAMLPGTTN
HHHCCCEEEECCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEECCCCCC
GKERMQAFLQDYTPQLPASCVCSGKALVIGEQISANTIRDFLIEDLNYSNVNVATFFAFS
HHHHHHHHHHHCCCCCCHHHEECCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
RTIAREGDIKLSNEGHLQKLIEEGCYDIIVGDPLFERFTNRQQIFVSLPHPAVSSKLHWQ
HHHHCCCCEEECCCHHHHHHHHCCCEEEEECCHHHHHHCCCEEEEEECCCCHHCCCHHHH
SYVSLLSNDFSKYLYEKINRKYPVTLQ
HHHHHHHHHHHHHHHHHHCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA