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The map label for this gene is gloA [C]

Identifier: 124485134

GI number: 124485134

Start: 293607

End: 293981

Strand: Reverse

Name: gloA [C]

Synonym: Mlab_0307

Alternate gene names: 124485134

Gene position: 293981-293607 (Counterclockwise)

Preceding gene: 124485140

Following gene: 124485133

Centisome position: 16.29

GC content: 52.0

Gene sequence:

>375_bases
ATGCAGTTTCGGATGATCCACAACAATATCAACGTGCTCGACCTCGACCGGTCGATGAAGTTCTATGCAGAAGCTTTGGG
ACTTTTTGAAGTAAAACGGATCGTGCCCGAGTCAGGGGACGTTACGATCGTCTTTTTAGGCGACGGACAGTCGGATCACA
AACTGGAACTCACGCAGGTTACCGGCCGAACAAAACCGTATGAGCCGGGCGACAACGGAAGACATCTGGCGTTCTCGACC
GATGATTTTCAGGGCTCGCTCGAAAAACACCAGAAGATGGGCTGCGTGAAGTCGGTCAACGAGGCAAGTGGTATCTATTA
CCTCGAGGATCCGGACGGATACACGATCGAAATCCTGCCAATCGGCAGAGGATGA

Upstream 100 bases:

>100_bases
AACCTTTTCATCTAAGTCCAAATAATATACGTGAGATTTTATCTCAACATTTTTTTCTGATCCACGGATCGCTTAAATAT
TTTAAAAGAGAGGTATCTGT

Downstream 100 bases:

>100_bases
TATCATATATATGTATATCCAATTTGCGAAAAAATCACAAAAAAATTCGGCTCTTATGTATTCATAACATATATCTGATT
ACATTCTTCCGGCCTGAAAC

Product: hypothetical protein

Products: glutathione; methylglyoxal

Alternate protein names: Glyoxalase/Bleomycin Resistance Protein/Dioxygenase; Glyoxalase I; Lactoylglutathione Lyase-Like Lyase; Glyoxalase Family Protein; Lactoylglutathione Lyase-Like Protein

Number of amino acids: Translated: 124; Mature: 124

Protein sequence:

>124_residues
MQFRMIHNNINVLDLDRSMKFYAEALGLFEVKRIVPESGDVTIVFLGDGQSDHKLELTQVTGRTKPYEPGDNGRHLAFST
DDFQGSLEKHQKMGCVKSVNEASGIYYLEDPDGYTIEILPIGRG

Sequences:

>Translated_124_residues
MQFRMIHNNINVLDLDRSMKFYAEALGLFEVKRIVPESGDVTIVFLGDGQSDHKLELTQVTGRTKPYEPGDNGRHLAFST
DDFQGSLEKHQKMGCVKSVNEASGIYYLEDPDGYTIEILPIGRG
>Mature_124_residues
MQFRMIHNNINVLDLDRSMKFYAEALGLFEVKRIVPESGDVTIVFLGDGQSDHKLELTQVTGRTKPYEPGDNGRHLAFST
DDFQGSLEKHQKMGCVKSVNEASGIYYLEDPDGYTIEILPIGRG

Specific function: Catalyzes The Conversion Of Hemimercaptal, Formed From Methylglyoxal And Glutathione, To S-Lactoylglutathione. [C]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.4.1.5

Molecular weight: Translated: 13923; Mature: 13923

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFRMIHNNINVLDLDRSMKFYAEALGLFEVKRIVPESGDVTIVFLGDGQSDHKLELTQV
CEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEE
TGRTKPYEPGDNGRHLAFSTDDFQGSLEKHQKMGCVKSVNEASGIYYLEDPDGYTIEILP
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCHHHCCCCCCCEEEEECCCCCEEEEEE
IGRG
CCCC
>Mature Secondary Structure
MQFRMIHNNINVLDLDRSMKFYAEALGLFEVKRIVPESGDVTIVFLGDGQSDHKLELTQV
CEEEEEECCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEE
TGRTKPYEPGDNGRHLAFSTDDFQGSLEKHQKMGCVKSVNEASGIYYLEDPDGYTIEILP
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHCCHHHCCCCCCCEEEEECCCCCEEEEEE
IGRG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 20280 [C]

Specific activity: NA

Km value (mM): 0.0272 {methylglyoxal/glutathione} [C]

Substrates: (R)-S-lactoylglutathione

Specific reaction: (R)-S-lactoylglutathione = glutathione + methylglyoxal

General reaction: addition of RSH [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA