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The map label for this gene is purF [H]

Identifier: 124485029

GI number: 124485029

Start: 190688

End: 192091

Strand: Reverse

Name: purF [H]

Synonym: Mlab_0202

Alternate gene names: 124485029

Gene position: 192091-190688 (Counterclockwise)

Preceding gene: 124485030

Following gene: 124485028

Centisome position: 10.64

GC content: 52.28

Gene sequence:

>1404_bases
ATGTGCGGCATCACTGGCATTACCGATTCTACAGATGTATCGGGATCATTGTACTTGGCATTATATGCACTCCAGCACCG
GGGTCAGGAGAGTGCAGGCATTTGTACGTATGATGGTGAGACTGTACATAGATACAAAGGATACGGGCTCGTGTCCGAGG
TATTTAGCGGTCCAATCCTTGACGGGCTGAGAGGGAAAACAGGCATTGGTCATGTGAGATATCCAACGACCGGGGGATCG
AAACCGGAGAACATCCAGCCGTTCCATTTTATGTTTCGGGACCATTCTCTGTCGCTTGTCCACAACGGGAACCTGATCAA
TACCGACGAACTCAGATATGAATACGAAGGACACGGCCACATTTTTTCAACGACTTCAGACACCGAAGTCATTGCAGCGA
TCATCGCAAACGAGATCATCCACGACCACACGCCTGACGAAGCGATCAGTTACTGTATGCGAAAGATCAGCGGATCATAT
GTCGTGATCTTTATGCTCGACGGAATTCTGTATTCTTTCCGTGATCCGCTCGGCATCAAACCACTGTGCCTCGGAAAAAC
CGAGACGGGAGGGCATGTTCTCGCTTCCGAAAGCGTAGCATTGGATGCGATCGGGGCCGAATTTATCAGAGACATCCGCC
CCGGCGAGAGCGTTAGACTGCAGGGTGGGGAAGTTTTTTCCAGGCAAGTGATGAAAGCAAAATCCTGCGCTCACTGCATG
TTCGAGTATGTCTACTTCGCACGGGCCGATGCCGTCATTGACGGGGTCTCGGTCTACGATGTGCGCAGAAAAACCGGAGC
ACTCCTCGCAAGGGAAGCGCCGGCAAAAGCGGATCTTATCTCGCCCGTTCCGGATTCGGGAACGGCGGCGGCGACTGGAT
TTTCCCGTGAATCGGGCACCCCTTTCCGTGAAGCCCTGATCAAAAATCGGTACACGGGCCGGACATTCATTATGCCGAGC
CAGGAAAAACGCGAGATCGCTGTTCGGATGAAACTAAATCCGGTTCGCGGTCATATCAAAGACAAATCCGTGGTCTTAGT
CGACGACAGCATCGTTAGAGGAACGACCTCGAAAAAGATCCTGTCTCTTGTGCGCGAGTTCGGTGCAAAGGAGCTCCATC
TCAGAATCGCATCACCACCGATCATCGCCCCCTGTTATCTTGGAACGGATTTTCCAACAAGGGATGAACTGATCGCTTCG
ACGAAATCGGTCAGCCAAGTGGAAAAAGAGATCGATGCAACCAGTCTTGCGCACATCTCGCTTGACGGACTCATCAAAGC
GATCGGCATTCCGCGACAGGATCTCTGTCTTGGATGTCTGACCGGGAAATACCCGCTGGCAATTCCGGGAGAAAAAGAGG
ACGACCGCGAGATAGAGATGGTAGACTCCTACTCCTGCCCCTGA

Upstream 100 bases:

>100_bases
GCTGCCGAGACGGATCAAGACGGCAACTGGACAACCGTTGTCGCAACGGCGGTCTTCGTCTGTTAAACCTATATCTGAAT
ACAACCAATATTTTTACAAC

Downstream 100 bases:

>100_bases
AAATATCCAAAATCACTTTTTTTAGAGAAAAACTGTTCCATACAGAGTTATTGAACTAAACTGGTGCAGAAAAATAAGTA
TGAAGAAAAATTAGGTAAAC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MCGITGITDSTDVSGSLYLALYALQHRGQESAGICTYDGETVHRYKGYGLVSEVFSGPILDGLRGKTGIGHVRYPTTGGS
KPENIQPFHFMFRDHSLSLVHNGNLINTDELRYEYEGHGHIFSTTSDTEVIAAIIANEIIHDHTPDEAISYCMRKISGSY
VVIFMLDGILYSFRDPLGIKPLCLGKTETGGHVLASESVALDAIGAEFIRDIRPGESVRLQGGEVFSRQVMKAKSCAHCM
FEYVYFARADAVIDGVSVYDVRRKTGALLAREAPAKADLISPVPDSGTAAATGFSRESGTPFREALIKNRYTGRTFIMPS
QEKREIAVRMKLNPVRGHIKDKSVVLVDDSIVRGTTSKKILSLVREFGAKELHLRIASPPIIAPCYLGTDFPTRDELIAS
TKSVSQVEKEIDATSLAHISLDGLIKAIGIPRQDLCLGCLTGKYPLAIPGEKEDDREIEMVDSYSCP

Sequences:

>Translated_467_residues
MCGITGITDSTDVSGSLYLALYALQHRGQESAGICTYDGETVHRYKGYGLVSEVFSGPILDGLRGKTGIGHVRYPTTGGS
KPENIQPFHFMFRDHSLSLVHNGNLINTDELRYEYEGHGHIFSTTSDTEVIAAIIANEIIHDHTPDEAISYCMRKISGSY
VVIFMLDGILYSFRDPLGIKPLCLGKTETGGHVLASESVALDAIGAEFIRDIRPGESVRLQGGEVFSRQVMKAKSCAHCM
FEYVYFARADAVIDGVSVYDVRRKTGALLAREAPAKADLISPVPDSGTAAATGFSRESGTPFREALIKNRYTGRTFIMPS
QEKREIAVRMKLNPVRGHIKDKSVVLVDDSIVRGTTSKKILSLVREFGAKELHLRIASPPIIAPCYLGTDFPTRDELIAS
TKSVSQVEKEIDATSLAHISLDGLIKAIGIPRQDLCLGCLTGKYPLAIPGEKEDDREIEMVDSYSCP
>Mature_467_residues
MCGITGITDSTDVSGSLYLALYALQHRGQESAGICTYDGETVHRYKGYGLVSEVFSGPILDGLRGKTGIGHVRYPTTGGS
KPENIQPFHFMFRDHSLSLVHNGNLINTDELRYEYEGHGHIFSTTSDTEVIAAIIANEIIHDHTPDEAISYCMRKISGSY
VVIFMLDGILYSFRDPLGIKPLCLGKTETGGHVLASESVALDAIGAEFIRDIRPGESVRLQGGEVFSRQVMKAKSCAHCM
FEYVYFARADAVIDGVSVYDVRRKTGALLAREAPAKADLISPVPDSGTAAATGFSRESGTPFREALIKNRYTGRTFIMPS
QEKREIAVRMKLNPVRGHIKDKSVVLVDDSIVRGTTSKKILSLVREFGAKELHLRIASPPIIAPCYLGTDFPTRDELIAS
TKSVSQVEKEIDATSLAHISLDGLIKAIGIPRQDLCLGCLTGKYPLAIPGEKEDDREIEMVDSYSCP

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=504, Percent_Identity=38.4920634920635, Blast_Score=328, Evalue=7e-90,
Organism=Escherichia coli, GI1788651, Length=455, Percent_Identity=43.0769230769231, Blast_Score=337, Evalue=7e-94,
Organism=Escherichia coli, GI1790167, Length=210, Percent_Identity=30.952380952381, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=481, Percent_Identity=38.6694386694387, Blast_Score=301, Evalue=4e-82,
Organism=Saccharomyces cerevisiae, GI6323958, Length=489, Percent_Identity=38.241308793456, Blast_Score=281, Evalue=1e-76,
Organism=Drosophila melanogaster, GI28573187, Length=491, Percent_Identity=40.9368635437882, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24659598, Length=471, Percent_Identity=41.8259023354565, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24659604, Length=464, Percent_Identity=42.2413793103448, Blast_Score=347, Evalue=8e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 51039; Mature: 51039

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGITDSTDVSGSLYLALYALQHRGQESAGICTYDGETVHRYKGYGLVSEVFSGPIL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHCCCCH
DGLRGKTGIGHVRYPTTGGSKPENIQPFHFMFRDHSLSLVHNGNLINTDELRYEYEGHGH
HCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCEECHHHEEEEECCCCE
IFSTTSDTEVIAAIIANEIIHDHTPDEAISYCMRKISGSYVVIFMLDGILYSFRDPLGIK
EEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCC
PLCLGKTETGGHVLASESVALDAIGAEFIRDIRPGESVRLQGGEVFSRQVMKAKSCAHCM
EEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHH
FEYVYFARADAVIDGVSVYDVRRKTGALLAREAPAKADLISPVPDSGTAAATGFSRESGT
HHHHHHHHHHHHHCCCHHEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
PFREALIKNRYTGRTFIMPSQEKREIAVRMKLNPVRGHIKDKSVVLVDDSIVRGTTSKKI
HHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEHHCCCCCCCEEEEECCCHHCCCCHHHH
LSLVREFGAKELHLRIASPPIIAPCYLGTDFPTRDELIASTKSVSQVEKEIDATSLAHIS
HHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEEEE
LDGLIKAIGIPRQDLCLGCLTGKYPLAIPGEKEDDREIEMVDSYSCP
HHHHHHHHCCCHHHHEEEEECCCCCEECCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure
MCGITGITDSTDVSGSLYLALYALQHRGQESAGICTYDGETVHRYKGYGLVSEVFSGPIL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCHHHHHHCCCCH
DGLRGKTGIGHVRYPTTGGSKPENIQPFHFMFRDHSLSLVHNGNLINTDELRYEYEGHGH
HCCCCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCEECHHHEEEEECCCCE
IFSTTSDTEVIAAIIANEIIHDHTPDEAISYCMRKISGSYVVIFMLDGILYSFRDPLGIK
EEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHCCCCCCC
PLCLGKTETGGHVLASESVALDAIGAEFIRDIRPGESVRLQGGEVFSRQVMKAKSCAHCM
EEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHH
FEYVYFARADAVIDGVSVYDVRRKTGALLAREAPAKADLISPVPDSGTAAATGFSRESGT
HHHHHHHHHHHHHCCCHHEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCC
PFREALIKNRYTGRTFIMPSQEKREIAVRMKLNPVRGHIKDKSVVLVDDSIVRGTTSKKI
HHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEHHCCCCCCCEEEEECCCHHCCCCHHHH
LSLVREFGAKELHLRIASPPIIAPCYLGTDFPTRDELIASTKSVSQVEKEIDATSLAHIS
HHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCHHHEEEEE
LDGLIKAIGIPRQDLCLGCLTGKYPLAIPGEKEDDREIEMVDSYSCP
HHHHHHHHCCCHHHHEEEEECCCCCEECCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]