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The map label for this gene is garL [H]

Identifier: 124266167

GI number: 124266167

Start: 1037332

End: 1038120

Strand: Direct

Name: garL [H]

Synonym: Mpe_A0974

Alternate gene names: 124266167

Gene position: 1037332-1038120 (Clockwise)

Preceding gene: 124266166

Following gene: 124266168

Centisome position: 25.65

GC content: 69.71

Gene sequence:

>789_bases
ATGAGACCCAACCGCCTGCGCGAGATCTGGAAGAGCGGCGGCGCCGCCCTGAACGGCTGGCTCGCCATCCCCAACAGCTT
CTCGGCCGAGACCATGGCCCACCAGGGCTGGGACGCACTCACCATCGACCTGCAGCACGGCGTGGTCGACTACCAGGCGA
TGGTCCCGATGCTGCAGGCCCTCTCCACCACGGCCACCGTGCCGGTGGTGCGCGTGCCCTGGCTCGAGCCCGGCATCCTG
ATGAAGACGCTGGATGCCGGCGCCTACGGCGTGATCTGCCCGATGGTCAACACCCGCGAGGACGCGCAGAAGCTGGTGGC
GTACACCCATTACGCGCCGCGCGGAACGCGCAGCTTCGGCCCGGTGCGCGCGCTGCTGTACGGTGGCGCCGACTACGCGC
AGCACGCCAACGACACCATCGTCACCTTCGCGATGATCGAGACCGCGCAGGCGCTCGACAACCTGGACGACATCCTGTCG
GTCGAAGGTCTCGACGCCATCTACATCGGGCCCTCGGACCTGTCGCTGGCGCTCGGCTGCACGCCGACCTTCGACGACGT
GGACCCGCCGGTGGCGCAGGCGATCGACCACATCCTGGCGCGCGCCAAGGCGCACGGCGTGGTGGCCGCGATCCACAACG
GCAGCCCCGAGGCCGCGCTCGCGCGCATCGCCAAGGGTTTCCAGTTCGTCACTGTGAGCTCCGACGCGCGGCTCATGGCC
GCTGGCGCGCAGCAGGTGGTGGCGAAGATGCGCGACCGCCCGACGTCGGGCAGCGCACAGGGCTATTGA

Upstream 100 bases:

>100_bases
GACAACCTGGCGCTGTTCTTCGCCGAGGGCCGGCTGGTGACGGCCGCGCCGCTGGACTGATCGATCAACGAACGGACCCA
CGAACGCCGAGAGGATGAAC

Downstream 100 bases:

>100_bases
TCCACGCACAGAGAAGGACCTCACCATGAAGATCGGATTCATCGGCCTGGGCATCATGGGCACGCCGATGGCGCTGCACC
TGGCGGCCGCGGGCCACGCG

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MRPNRLREIWKSGGAALNGWLAIPNSFSAETMAHQGWDALTIDLQHGVVDYQAMVPMLQALSTTATVPVVRVPWLEPGIL
MKTLDAGAYGVICPMVNTREDAQKLVAYTHYAPRGTRSFGPVRALLYGGADYAQHANDTIVTFAMIETAQALDNLDDILS
VEGLDAIYIGPSDLSLALGCTPTFDDVDPPVAQAIDHILARAKAHGVVAAIHNGSPEAALARIAKGFQFVTVSSDARLMA
AGAQQVVAKMRDRPTSGSAQGY

Sequences:

>Translated_262_residues
MRPNRLREIWKSGGAALNGWLAIPNSFSAETMAHQGWDALTIDLQHGVVDYQAMVPMLQALSTTATVPVVRVPWLEPGIL
MKTLDAGAYGVICPMVNTREDAQKLVAYTHYAPRGTRSFGPVRALLYGGADYAQHANDTIVTFAMIETAQALDNLDDILS
VEGLDAIYIGPSDLSLALGCTPTFDDVDPPVAQAIDHILARAKAHGVVAAIHNGSPEAALARIAKGFQFVTVSSDARLMA
AGAQQVVAKMRDRPTSGSAQGY
>Mature_262_residues
MRPNRLREIWKSGGAALNGWLAIPNSFSAETMAHQGWDALTIDLQHGVVDYQAMVPMLQALSTTATVPVVRVPWLEPGIL
MKTLDAGAYGVICPMVNTREDAQKLVAYTHYAPRGTRSFGPVRALLYGGADYAQHANDTIVTFAMIETAQALDNLDDILS
VEGLDAIYIGPSDLSLALGCTPTFDDVDPPVAQAIDHILARAKAHGVVAAIHNGSPEAALARIAKGFQFVTVSSDARLMA
AGAQQVVAKMRDRPTSGSAQGY

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=248, Percent_Identity=37.5, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788578, Length=251, Percent_Identity=33.4661354581673, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 27926; Mature: 27926

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNRLREIWKSGGAALNGWLAIPNSFSAETMAHQGWDALTIDLQHGVVDYQAMVPMLQA
CCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
LSTTATVPVVRVPWLEPGILMKTLDAGAYGVICPMVNTREDAQKLVAYTHYAPRGTRSFG
HHHHCCCCEEEECCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
PVRALLYGGADYAQHANDTIVTFAMIETAQALDNLDDILSVEGLDAIYIGPSDLSLALGC
HHHHHHCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEC
TPTFDDVDPPVAQAIDHILARAKAHGVVAAIHNGSPEAALARIAKGFQFVTVSSDARLMA
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCEEEH
AGAQQVVAKMRDRPTSGSAQGY
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRPNRLREIWKSGGAALNGWLAIPNSFSAETMAHQGWDALTIDLQHGVVDYQAMVPMLQA
CCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHH
LSTTATVPVVRVPWLEPGILMKTLDAGAYGVICPMVNTREDAQKLVAYTHYAPRGTRSFG
HHHHCCCCEEEECCCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCC
PVRALLYGGADYAQHANDTIVTFAMIETAQALDNLDDILSVEGLDAIYIGPSDLSLALGC
HHHHHHCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEC
TPTFDDVDPPVAQAIDHILARAKAHGVVAAIHNGSPEAALARIAKGFQFVTVSSDARLMA
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCEEEEEECCCCEEEH
AGAQQVVAKMRDRPTSGSAQGY
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA