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The map label for this gene is pilD [H]

Identifier: 124265700

GI number: 124265700

Start: 545171

End: 546022

Strand: Reverse

Name: pilD [H]

Synonym: Mpe_A0507

Alternate gene names: 124265700

Gene position: 546022-545171 (Counterclockwise)

Preceding gene: 124265701

Following gene: 124265699

Centisome position: 13.5

GC content: 67.49

Gene sequence:

>852_bases
ATGTCGTCCGACCTGCAGGGCGTGCTGCTGTCGCCCTGGGCTCTGGGGCTGCTCGGGCTGTGCATCGGCAGCTTTCTCAA
CGTCGTCATCCACCGCCTGCCACAGATGATGGAGCGGGCCTGGCGACTCGAAAGCGCCGAGCTGCTCGGGGTCACGGCCC
CAGAATCTCCCGCGCTCACGCTGGCGAAACCCGCCTCGCGCTGCCCGCAATGCGGTCATCGCATCCGCTGGTTCGAGAAT
GTCCCGGTGCTGAGCTGGCTGCTGTTGCGCGGACGCTGCTCAGCCTGCAAGACGCCGATCTCTCCGCGCTATCCTCTCTT
CGAGATCGCGACAGGGCTGTTGTTCGCCTCGCTGGCCTGGCGTTTCGGCGCGCAACCGACGGTGCTGGCCTGGTGCGCCT
TCGTCGCCGTCCTGCTGGCACTGTCCGGCATCGACTGGGACACGACGCTCCTGCCCGACAGCCTCACCTTGCCACTGCTC
TGGGCCGGGCTGGTGGCTGCCGCGCTCGGCTGGACGCTGCCGCTCTCGACGTCCGTCTGGGGCGCGGTGTTCGGTTATTT
GTCGCTGTGGTCGGTGTACTGGCTGTTCAAGCTCACGACTGGCAAGGAAGGCATGGGCGCGGGCGACTTCAAGCTGCTGG
CCGCCCTCGGTGCCTGGCTCGGATGGCAGATGCTGCTTCCCATCGTGCTCGGAGCTTCGTTGATCGGTGCCATCGTCGGC
ATCGCCATGAAGCTGGCTGGCGCTCTGCACGAAGGTCGCTACGTGCCCTTCGGCCCCTTCCTGGCCGGTGCCGGACTGGT
CGTCCTGTACGCAGGCGCCGAGCGCGTGATGGGCTGGCTCGGCTGGGCCTGA

Upstream 100 bases:

>100_bases
TCATCATCGTCGTGCTGGGCACCATCATCGGCGGCATCGTGGTCTCGATGTACCTGCCGATCTTCAAGCTCGGCCAGGTG
GTCTGAGCACGCCCGGAGGC

Downstream 100 bases:

>100_bases
GGCTCGATGGGCAGCGAACGCATAAGCTTGCGCATCGGTCTCACCGGCGGCATCGGCAGTGGCAAGAGTACGGTGGCCGC
GCTGCTCGAAGGGCGCGGCG

Product: signal peptidase

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSSDLQGVLLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVTAPESPALTLAKPASRCPQCGHRIRWFEN
VPVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFASLAWRFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLL
WAGLVAAALGWTLPLSTSVWGAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAIVG
IAMKLAGALHEGRYVPFGPFLAGAGLVVLYAGAERVMGWLGWA

Sequences:

>Translated_283_residues
MSSDLQGVLLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVTAPESPALTLAKPASRCPQCGHRIRWFEN
VPVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFASLAWRFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLL
WAGLVAAALGWTLPLSTSVWGAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAIVG
IAMKLAGALHEGRYVPFGPFLAGAGLVVLYAGAERVMGWLGWA
>Mature_282_residues
SSDLQGVLLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVTAPESPALTLAKPASRCPQCGHRIRWFENV
PVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFASLAWRFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLLW
AGLVAAALGWTLPLSTSVWGAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAIVGI
AMKLAGALHEGRYVPFGPFLAGAGLVVLYAGAERVMGWLGWA

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=256, Percent_Identity=39.84375, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1789732, Length=139, Percent_Identity=48.2014388489209, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 30445; Mature: 30313

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDLQGVLLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVTAPESPALT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCEE
LAKPASRCPQCGHRIRWFENVPVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFASLAW
ECCHHHHCHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLLWAGLVAAALGWTLPLSTSVW
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHH
GAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAIVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAMKLAGALHEGRYVPFGPFLAGAGLVVLYAGAERVMGWLGWA
HHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCHHHHHHHHCCC
>Mature Secondary Structure 
SSDLQGVLLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVTAPESPALT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCEE
LAKPASRCPQCGHRIRWFENVPVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFASLAW
ECCHHHHCHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCCCCCHHHHHHHHHHHHHHH
RFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLLWAGLVAAALGWTLPLSTSVW
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHH
GAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAIVG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAMKLAGALHEGRYVPFGPFLAGAGLVVLYAGAERVMGWLGWA
HHHHHHHHHHCCCCCCCCHHHHCCCCEEEECCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]