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The map label for this gene is rep [H]

Identifier: 124265321

GI number: 124265321

Start: 130635

End: 132716

Strand: Reverse

Name: rep [H]

Synonym: Mpe_A0128

Alternate gene names: 124265321

Gene position: 132716-130635 (Counterclockwise)

Preceding gene: 124265326

Following gene: 124265320

Centisome position: 3.28

GC content: 71.09

Gene sequence:

>2082_bases
ATGCAGGCGGTTCACCACCTGGCCGGCCCCTGCCTGGTGCTGGCCGGCGCCGGCTCGGGCAAGACGCGCGTGATCACGCA
CAAGATCGCGCGCCTGCTGGAGGCCGGGCTGGCTCCGTCGCAGATCGCCGCCATCACCTTCACCAACAAGGCGGCGGCCG
AGATGCGCGAGCGCGCCAAGGCCCTGGTGGGGGCGCGGCCGGCCAAGGGCCTGCTGATCAGCACCTTCCACTCGCTCGGC
GTGCGGCTGCTGCGCGAGGACGGCGCGGCGCTGGGCCTGAAGCCGCAGTTCTCGATCCTCGACAGCGACGACGTGCTGGG
CATCCTGCGCGACGCCGGCGGCACCACCGACGCCAAGACCGCGCGGCGCTGGCAGTGGGCCATCAGCCTCTGGAAGAACC
AGGGCCTGACGCCGGCCCAGGCCGAAGCCGAGGCGAAGAACCCCGACGAGGTGCAGGCCGCGCGCGTGATGACGCGCTAC
CAGGAGCGGCTGGCCGCCTACCAGGCGGTGGACTTCGACGACCTGATCGGCCTGCCGGTGGCGCTGCTGCAGCGGCACGA
CGTGGTGCGCGCGAAGTGGCAGGCCGCGCTGCGCCACGTGCTGGTCGACGAGTACCAGGACACCAACGCGCAGCAGTACG
AACTGCTCAAGCTGCTGGTCGGCGACGGCCAGGGCGGGCGCGACCGGCCGGCGATGTTCACCGCGGTCGGCGACGACGAC
CAGAGCATCTACGGCTGGCGCGGCGCGACGATCGACAACCTCAAGCGCCTGCCGCAGGACTACCCGGACCTGCAGGTGAT
CCCGCTGGAGCAGAACTACCGCTCGACCGGCAACATCCTGCGCGCCGCCAACCACGTGATCGCGGCCAACCCGAAGATCT
TCGACAAGAAGCTGTGGAGCGAATTCGGCGACGGCGAGCCGGTGCGCGTGGTCGAGAGCGACAGCGAGGAACACGAGGCC
GAGCGCGCGGTCGCGCGCATCCAGGCGCTGCGCGGCCAGGGCGGCGCGGCGGCCGGCGCGGTGGGCGGGCAGGGCATCGA
GTGGAAGGACTTCGCGATCCTCTACCGCGCCAACCACCAGGCGCGCGTGTTCGAGCAGGCCCTGCGCAAGGCGCAGATCC
CGTACAAGGTGTCGGGCGGCCAGAGCTATTTCGAGCGCGCCGAGATCAAGGACCTGTGCGCCTGGTTGCGCCTGCTGGTG
AACCAAGACGACGACCCCGCCTTCCTGCGCGCCGTGACGACGCCCAAGCGCGGCATCGGCCACCAGACGCTGGCCGGCCT
GGGCGAGTTCGCCGGCCGCTGGAAGGTGTCTCTCTACGAAGCGCTGTTCGCGGAATCGCTCGGCACGGTGCTCAGCGCGA
AGGCGGTCGGCTCGCTCCACGAGTTCGGCCGTTGCGTCAACGACCTCGAGTACCGCGCACGCCAGACCGTCGGTGCCGAG
GATGCGCGCGCGCTGCTGATGGGCTGGCTGAAGGACATCGGCTACGAGCAGCATCTGCACGACGGCGAGGACAGCGAGAA
GCTGGCCGCGGCGCGCTGGTCGAACGTGCTCGACTTCGTCGAGTGGATCGCCAGGCGCTGCGGCGGCGAGACGGCCGACG
ACGGCAGCGGCCAGGAGAGCGAGCGGCGCAGCGTGCTCGAAGTGGCGCAGACCATCGCGGTGATCATCAGCCTGGCCGAG
CGCGGCGACGATCAGAACGTGCTGACGCTCTCCACGCTGCACGCTGCCAAGGGGCTGGAGTGGCCGCACGTGGTGCTGGC
CGGCGTCAACGAGGGCCTGCTGCCGTTCAAGACCGACGACGAATCGCTCACCGCGGCAGCGCTGGTACAGCGGCTGGAGG
AGGAGCGGCGATTGATGTACGTGGGCATCACCCGCGCCCGCAGCACCCTGGCGGTCAGCTGGCTCAAGCGCCGCAAGCGC
GGCCGCGAGACCGTGGCCGGCGCGCCCAGCCGCTTCATCGCCGAGATGAAGCTGGAAGAAGGCCGCGTGAAGGAAGACCC
GCGCGAGAAGCTGAAGGCGCTGCGGGCGGCGTTCGCGGCCAAGGCCGCGCAGCCGGCGTCCGGCCCGACCGCCGCGGACT
GA

Upstream 100 bases:

>100_bases
GCTTGGCTATCATCGCGCGCCCCGCCGTTCGCCACGGCTCCGTCCCGTCGTCTCCGCTCCGTCCTCCTTGTCCGCGCCTG
TCTCGCTCAACACCGCCCAG

Downstream 100 bases:

>100_bases
GTGCGCCGCGCGCGCCGAGGGCCTTATGCCGACGGCGCGAAAGCAAACAACGAAACGGTCGTTCCCTCGCGCGCGGCCAG
CCCGGACACTCGGAAACTTT

Product: putative ATP-dependent DNA helicase rep protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 693; Mature: 693

Protein sequence:

>693_residues
MQAVHHLAGPCLVLAGAGSGKTRVITHKIARLLEAGLAPSQIAAITFTNKAAAEMRERAKALVGARPAKGLLISTFHSLG
VRLLREDGAALGLKPQFSILDSDDVLGILRDAGGTTDAKTARRWQWAISLWKNQGLTPAQAEAEAKNPDEVQAARVMTRY
QERLAAYQAVDFDDLIGLPVALLQRHDVVRAKWQAALRHVLVDEYQDTNAQQYELLKLLVGDGQGGRDRPAMFTAVGDDD
QSIYGWRGATIDNLKRLPQDYPDLQVIPLEQNYRSTGNILRAANHVIAANPKIFDKKLWSEFGDGEPVRVVESDSEEHEA
ERAVARIQALRGQGGAAAGAVGGQGIEWKDFAILYRANHQARVFEQALRKAQIPYKVSGGQSYFERAEIKDLCAWLRLLV
NQDDDPAFLRAVTTPKRGIGHQTLAGLGEFAGRWKVSLYEALFAESLGTVLSAKAVGSLHEFGRCVNDLEYRARQTVGAE
DARALLMGWLKDIGYEQHLHDGEDSEKLAAARWSNVLDFVEWIARRCGGETADDGSGQESERRSVLEVAQTIAVIISLAE
RGDDQNVLTLSTLHAAKGLEWPHVVLAGVNEGLLPFKTDDESLTAAALVQRLEEERRLMYVGITRARSTLAVSWLKRRKR
GRETVAGAPSRFIAEMKLEEGRVKEDPREKLKALRAAFAAKAAQPASGPTAAD

Sequences:

>Translated_693_residues
MQAVHHLAGPCLVLAGAGSGKTRVITHKIARLLEAGLAPSQIAAITFTNKAAAEMRERAKALVGARPAKGLLISTFHSLG
VRLLREDGAALGLKPQFSILDSDDVLGILRDAGGTTDAKTARRWQWAISLWKNQGLTPAQAEAEAKNPDEVQAARVMTRY
QERLAAYQAVDFDDLIGLPVALLQRHDVVRAKWQAALRHVLVDEYQDTNAQQYELLKLLVGDGQGGRDRPAMFTAVGDDD
QSIYGWRGATIDNLKRLPQDYPDLQVIPLEQNYRSTGNILRAANHVIAANPKIFDKKLWSEFGDGEPVRVVESDSEEHEA
ERAVARIQALRGQGGAAAGAVGGQGIEWKDFAILYRANHQARVFEQALRKAQIPYKVSGGQSYFERAEIKDLCAWLRLLV
NQDDDPAFLRAVTTPKRGIGHQTLAGLGEFAGRWKVSLYEALFAESLGTVLSAKAVGSLHEFGRCVNDLEYRARQTVGAE
DARALLMGWLKDIGYEQHLHDGEDSEKLAAARWSNVLDFVEWIARRCGGETADDGSGQESERRSVLEVAQTIAVIISLAE
RGDDQNVLTLSTLHAAKGLEWPHVVLAGVNEGLLPFKTDDESLTAAALVQRLEEERRLMYVGITRARSTLAVSWLKRRKR
GRETVAGAPSRFIAEMKLEEGRVKEDPREKLKALRAAFAAKAAQPASGPTAAD
>Mature_693_residues
MQAVHHLAGPCLVLAGAGSGKTRVITHKIARLLEAGLAPSQIAAITFTNKAAAEMRERAKALVGARPAKGLLISTFHSLG
VRLLREDGAALGLKPQFSILDSDDVLGILRDAGGTTDAKTARRWQWAISLWKNQGLTPAQAEAEAKNPDEVQAARVMTRY
QERLAAYQAVDFDDLIGLPVALLQRHDVVRAKWQAALRHVLVDEYQDTNAQQYELLKLLVGDGQGGRDRPAMFTAVGDDD
QSIYGWRGATIDNLKRLPQDYPDLQVIPLEQNYRSTGNILRAANHVIAANPKIFDKKLWSEFGDGEPVRVVESDSEEHEA
ERAVARIQALRGQGGAAAGAVGGQGIEWKDFAILYRANHQARVFEQALRKAQIPYKVSGGQSYFERAEIKDLCAWLRLLV
NQDDDPAFLRAVTTPKRGIGHQTLAGLGEFAGRWKVSLYEALFAESLGTVLSAKAVGSLHEFGRCVNDLEYRARQTVGAE
DARALLMGWLKDIGYEQHLHDGEDSEKLAAARWSNVLDFVEWIARRCGGETADDGSGQESERRSVLEVAQTIAVIISLAE
RGDDQNVLTLSTLHAAKGLEWPHVVLAGVNEGLLPFKTDDESLTAAALVQRLEEERRLMYVGITRARSTLAVSWLKRRKR
GRETVAGAPSRFIAEMKLEEGRVKEDPREKLKALRAAFAAKAAQPASGPTAAD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=664, Percent_Identity=45.3313253012048, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI2367296, Length=663, Percent_Identity=37.7073906485671, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787196, Length=331, Percent_Identity=29.0030211480363, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=733, Percent_Identity=25.9208731241473, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75964; Mature: 75964

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAVHHLAGPCLVLAGAGSGKTRVITHKIARLLEAGLAPSQIAAITFTNKAAAEMRERAK
CCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
ALVGARPAKGLLISTFHSLGVRLLREDGAALGLKPQFSILDSDDVLGILRDAGGTTDAKT
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCHHHHHHHCCCCCCCHHH
ARRWQWAISLWKNQGLTPAQAEAEAKNPDEVQAARVMTRYQERLAAYQAVDFDDLIGLPV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALLQRHDVVRAKWQAALRHVLVDEYQDTNAQQYELLKLLVGDGQGGRDRPAMFTAVGDDD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
QSIYGWRGATIDNLKRLPQDYPDLQVIPLEQNYRSTGNILRAANHVIAANPKIFDKKLWS
CCEECCCCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCEEECCCCHHHHHHHH
EFGDGEPVRVVESDSEEHEAERAVARIQALRGQGGAAAGAVGGQGIEWKDFAILYRANHQ
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCH
ARVFEQALRKAQIPYKVSGGQSYFERAEIKDLCAWLRLLVNQDDDPAFLRAVTTPKRGIG
HHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCC
HQTLAGLGEFAGRWKVSLYEALFAESLGTVLSAKAVGSLHEFGRCVNDLEYRARQTVGAE
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DARALLMGWLKDIGYEQHLHDGEDSEKLAAARWSNVLDFVEWIARRCGGETADDGSGQES
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ERRSVLEVAQTIAVIISLAERGDDQNVLTLSTLHAAKGLEWPHVVLAGVNEGLLPFKTDD
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCEEEECCCCCCCCCCCCC
ESLTAAALVQRLEEERRLMYVGITRARSTLAVSWLKRRKRGRETVAGAPSRFIAEMKLEE
HHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHC
GRVKEDPREKLKALRAAFAAKAAQPASGPTAAD
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQAVHHLAGPCLVLAGAGSGKTRVITHKIARLLEAGLAPSQIAAITFTNKAAAEMRERAK
CCCHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
ALVGARPAKGLLISTFHSLGVRLLREDGAALGLKPQFSILDSDDVLGILRDAGGTTDAKT
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCEEECCCCHHHHHHHCCCCCCCHHH
ARRWQWAISLWKNQGLTPAQAEAEAKNPDEVQAARVMTRYQERLAAYQAVDFDDLIGLPV
HHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALLQRHDVVRAKWQAALRHVLVDEYQDTNAQQYELLKLLVGDGQGGRDRPAMFTAVGDDD
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
QSIYGWRGATIDNLKRLPQDYPDLQVIPLEQNYRSTGNILRAANHVIAANPKIFDKKLWS
CCEECCCCCCHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCEEECCCCHHHHHHHH
EFGDGEPVRVVESDSEEHEAERAVARIQALRGQGGAAAGAVGGQGIEWKDFAILYRANHQ
HCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCH
ARVFEQALRKAQIPYKVSGGQSYFERAEIKDLCAWLRLLVNQDDDPAFLRAVTTPKRGIG
HHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCCC
HQTLAGLGEFAGRWKVSLYEALFAESLGTVLSAKAVGSLHEFGRCVNDLEYRARQTVGAE
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
DARALLMGWLKDIGYEQHLHDGEDSEKLAAARWSNVLDFVEWIARRCGGETADDGSGQES
HHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ERRSVLEVAQTIAVIISLAERGDDQNVLTLSTLHAAKGLEWPHVVLAGVNEGLLPFKTDD
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHCCCCCCCEEEECCCCCCCCCCCCC
ESLTAAALVQRLEEERRLMYVGITRARSTLAVSWLKRRKRGRETVAGAPSRFIAEMKLEE
HHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHC
GRVKEDPREKLKALRAAFAAKAAQPASGPTAAD
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]