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The map label for this gene is priA [H]

Identifier: 124265326

GI number: 124265326

Start: 136848

End: 138995

Strand: Reverse

Name: priA [H]

Synonym: Mpe_A0133

Alternate gene names: 124265326

Gene position: 138995-136848 (Counterclockwise)

Preceding gene: 124265327

Following gene: 124265321

Centisome position: 3.44

GC content: 74.86

Gene sequence:

>2148_bases
TTGGGCAAGTCACCTTCATCGCGAGCGGCCGCCACATCTGCACCGGCGGCGGGCGCTGAGCCCCCGGCGCCCGCCCGCGA
GCCGGTCGTGGTGCCGGTGGTGATCGACGCACCGCAGTACAGCGGCCTGAAGGCGGCGCTCGGATATTTGAGCGAGCGCC
CGCTCACGCCAGGCACCCTGCTGCGCGTGCCGCTGGGTCGCCGTGAAGTGCTGGGCGTGGCCTGGGACACGCGCTCCGGT
GAGGACTCGGCGGTGCCCGATGGCGTGACGTTGCGCGAGGTGGCCGCGGTGTTCGACGCGCTGCCGCCGCTGCCGGCCGC
CTGGCGCGAGCTGGTGGACTTTGCTGCCTCGTACTACCAGCGCAGCCCCGGCGAGGTGGCGCTGGCGGTGCTGCCGCCCG
AACTGCGCATGTTGAGCGCCACGCAGCTGGAGCGCCGGCTCACGCGACTGCGCGCCAAGCTGCTGCCGCCCAGCGGCGCG
CAGGGCGCGCTGCCCGCCCCCAGCGCCGAGCAGGCGCAGGTGCTGCAAGCGCTGGCCGCGAGCGCGGGCGATGCCCCCGT
GACGCTGCTGCACGGCAGCACCGGCAGCGGCAAGACCGAGATCTACCTGCGCGAGGCGCAGCGCGTGGTCGAGGCCGGCC
GCCAGGCGCTGGTGCTGGTGCCGGAGATCAACCTCACGCCGCAGCTCGAAGAGCGCTTCCTGGCGCGCTTCCCAGGGCGG
CGGGTGGTGTCGCTGCACAGCGGCCTGACGCCGGCGCGTCGGCTGAAGCACTGGCTGATGGTGCTGCTGGGCGAGGCCGA
CCTCGTGCTCGGCACGCGGCTGGCGGTGTTCGCGCCGCTGCCGCGGCTGGGGCTGGTGATCGTCGACGAGGAGCACGACC
CGTCCTACAAGCAGCAGGAGGGGGCGCGCTACTCGGCCCGCGACCTGGCGGTGGTGCGCGGCCGCGTCGAGGGCGTGCCG
GTGCTGCTGGGCTCGGCCACGCCGTCGCTGGAGAGCTGGCACAACGCGCTTCCGGCGACCGAGGGCGGTGCCGGCCGCTA
CACCCGCCTGACCATGGCCGCGCGCATCGGCGGCGGCGTGCTGCCCACGGTGCGGCTGGTCGACATGAGCCGGCTGCCCA
AGGAGGTGCTGCGCGACAGCGCGCTGTCGCCCGCGCTGCTGGGCGCGCTGCGCGAGCGGCTGGCGCGCGGCGAGCAGAGC
CTGGTGCTGCTGAACCGCCGCGGCTACGCGCCGGTGCTGCATTGCACCGAGTGCGGCTGGAAGAGCGCCTGCGCGCACTG
CAGCGCCTGGCGGGTGTTCCACAAGCTCGACCGCACGCTGCGCTGCCATCACTGCGGCCTCACCGAGCGCGTGCCGCGCG
CCTGCCCGGACTGCGGCAATGCCGACATCCAGCCCACCGGCCGCGGCACCGAACGCCTGGAGGAGCAGCTGGCCCTGGCG
CTGCCGGGCGCACGCGTGGGTCGCATCGACGCCGACACCACGCGGCGCAGCGGCGCGCTGGAGGCCGAGCTGTCCCGCGT
GCACGCCGGCGAGGTGGACGTGCTGGTGGGCACGCAGATGATCGCCAAGGGCCACGACTTCCGCCGCATGACGCTGGTGG
CGGCGGTGAACCCCGACGCGGCGCTGTTCAGCAGCGACTTCCGCGCGCCGGAGCGCCAGTTCTCGTTGCTGATGCAGGCC
GCCGGTCGTGCCGGCCGTGCTGCGGAGCAGGCCGATCGCGCGGAGATGTGGGTGCAGACCTGGCACCCGCAGCACGCGCT
GTACGCGGCGCTGCGCACGCACGACTACGCGGCCTTCGCGCAGGCGCAGCTCGCCGAGCGGCGCGCGGCGGGCCTGCCGC
CCTACACCCACCTGGCGCTGCTGCGCGCCGACGCGCGCGAGCAGTCGGTGGCGCAGGCCTGGCTGCGGGTGGTGAGCGAC
GAGGCCCGCGAACTCGCCGAGCAGCACCAGGTGGCGCTCTACCCGCCGGTGCCGCTGAGCGTGCAGCGCGTGGCCGACAT
GGAGCGGGCGCAACTGCTGGTGGAGTCGGCCTCGCGGCCGGCGCTGCAGCGCTTTCTCACGGCCTGGGACGCCACGCTGC
GGCGCAAGCGGCCGGAGCAGAAGGGGCTGTTGCGCTGGGCCGTCGACGTGGACCCGCTGGCGATCTAG

Upstream 100 bases:

>100_bases
CAGGTTTCCCACAAAGTTTTCCACAGGGCACGTGGATAGCTCGAAAACCCCAAACGAATCAACGGCTTGGCCGCTTTCCT
TGATAAGAACTTGAGATTCC

Downstream 100 bases:

>100_bases
CGCCCGCGCAGGTGGTCGGGCGATTCGAGCCGCTTGGCCAGCGTGGACAGCAGCCAAGTGAGCAGCAGGTAGACCGCCGA
GATCGCGAGGTAGGGTTCCC

Product: replication restart DNA helicase PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MGKSPSSRAAATSAPAAGAEPPAPAREPVVVPVVIDAPQYSGLKAALGYLSERPLTPGTLLRVPLGRREVLGVAWDTRSG
EDSAVPDGVTLREVAAVFDALPPLPAAWRELVDFAASYYQRSPGEVALAVLPPELRMLSATQLERRLTRLRAKLLPPSGA
QGALPAPSAEQAQVLQALAASAGDAPVTLLHGSTGSGKTEIYLREAQRVVEAGRQALVLVPEINLTPQLEERFLARFPGR
RVVSLHSGLTPARRLKHWLMVLLGEADLVLGTRLAVFAPLPRLGLVIVDEEHDPSYKQQEGARYSARDLAVVRGRVEGVP
VLLGSATPSLESWHNALPATEGGAGRYTRLTMAARIGGGVLPTVRLVDMSRLPKEVLRDSALSPALLGALRERLARGEQS
LVLLNRRGYAPVLHCTECGWKSACAHCSAWRVFHKLDRTLRCHHCGLTERVPRACPDCGNADIQPTGRGTERLEEQLALA
LPGARVGRIDADTTRRSGALEAELSRVHAGEVDVLVGTQMIAKGHDFRRMTLVAAVNPDAALFSSDFRAPERQFSLLMQA
AGRAGRAAEQADRAEMWVQTWHPQHALYAALRTHDYAAFAQAQLAERRAAGLPPYTHLALLRADAREQSVAQAWLRVVSD
EARELAEQHQVALYPPVPLSVQRVADMERAQLLVESASRPALQRFLTAWDATLRRKRPEQKGLLRWAVDVDPLAI

Sequences:

>Translated_715_residues
MGKSPSSRAAATSAPAAGAEPPAPAREPVVVPVVIDAPQYSGLKAALGYLSERPLTPGTLLRVPLGRREVLGVAWDTRSG
EDSAVPDGVTLREVAAVFDALPPLPAAWRELVDFAASYYQRSPGEVALAVLPPELRMLSATQLERRLTRLRAKLLPPSGA
QGALPAPSAEQAQVLQALAASAGDAPVTLLHGSTGSGKTEIYLREAQRVVEAGRQALVLVPEINLTPQLEERFLARFPGR
RVVSLHSGLTPARRLKHWLMVLLGEADLVLGTRLAVFAPLPRLGLVIVDEEHDPSYKQQEGARYSARDLAVVRGRVEGVP
VLLGSATPSLESWHNALPATEGGAGRYTRLTMAARIGGGVLPTVRLVDMSRLPKEVLRDSALSPALLGALRERLARGEQS
LVLLNRRGYAPVLHCTECGWKSACAHCSAWRVFHKLDRTLRCHHCGLTERVPRACPDCGNADIQPTGRGTERLEEQLALA
LPGARVGRIDADTTRRSGALEAELSRVHAGEVDVLVGTQMIAKGHDFRRMTLVAAVNPDAALFSSDFRAPERQFSLLMQA
AGRAGRAAEQADRAEMWVQTWHPQHALYAALRTHDYAAFAQAQLAERRAAGLPPYTHLALLRADAREQSVAQAWLRVVSD
EARELAEQHQVALYPPVPLSVQRVADMERAQLLVESASRPALQRFLTAWDATLRRKRPEQKGLLRWAVDVDPLAI
>Mature_714_residues
GKSPSSRAAATSAPAAGAEPPAPAREPVVVPVVIDAPQYSGLKAALGYLSERPLTPGTLLRVPLGRREVLGVAWDTRSGE
DSAVPDGVTLREVAAVFDALPPLPAAWRELVDFAASYYQRSPGEVALAVLPPELRMLSATQLERRLTRLRAKLLPPSGAQ
GALPAPSAEQAQVLQALAASAGDAPVTLLHGSTGSGKTEIYLREAQRVVEAGRQALVLVPEINLTPQLEERFLARFPGRR
VVSLHSGLTPARRLKHWLMVLLGEADLVLGTRLAVFAPLPRLGLVIVDEEHDPSYKQQEGARYSARDLAVVRGRVEGVPV
LLGSATPSLESWHNALPATEGGAGRYTRLTMAARIGGGVLPTVRLVDMSRLPKEVLRDSALSPALLGALRERLARGEQSL
VLLNRRGYAPVLHCTECGWKSACAHCSAWRVFHKLDRTLRCHHCGLTERVPRACPDCGNADIQPTGRGTERLEEQLALAL
PGARVGRIDADTTRRSGALEAELSRVHAGEVDVLVGTQMIAKGHDFRRMTLVAAVNPDAALFSSDFRAPERQFSLLMQAA
GRAGRAAEQADRAEMWVQTWHPQHALYAALRTHDYAAFAQAQLAERRAAGLPPYTHLALLRADAREQSVAQAWLRVVSDE
ARELAEQHQVALYPPVPLSVQRVADMERAQLLVESASRPALQRFLTAWDATLRRKRPEQKGLLRWAVDVDPLAI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=549, Percent_Identity=43.1693989071038, Blast_Score=406, Evalue=1e-114,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 77551; Mature: 77420

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSPSSRAAATSAPAAGAEPPAPAREPVVVPVVIDAPQYSGLKAALGYLSERPLTPGTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCE
LRVPLGRREVLGVAWDTRSGEDSAVPDGVTLREVAAVFDALPPLPAAWRELVDFAASYYQ
EECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RSPGEVALAVLPPELRMLSATQLERRLTRLRAKLLPPSGAQGALPAPSAEQAQVLQALAA
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
SAGDAPVTLLHGSTGSGKTEIYLREAQRVVEAGRQALVLVPEINLTPQLEERFLARFPGR
CCCCCCEEEEECCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCC
RVVSLHSGLTPARRLKHWLMVLLGEADLVLGTRLAVFAPLPRLGLVIVDEEHDPSYKQQE
EEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHC
GARYSARDLAVVRGRVEGVPVLLGSATPSLESWHNALPATEGGAGRYTRLTMAARIGGGV
CCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHCCCC
LPTVRLVDMSRLPKEVLRDSALSPALLGALRERLARGEQSLVLLNRRGYAPVLHCTECGW
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCH
KSACAHCSAWRVFHKLDRTLRCHHCGLTERVPRACPDCGNADIQPTGRGTERLEEQLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
LPGARVGRIDADTTRRSGALEAELSRVHAGEVDVLVGTQMIAKGHDFRRMTLVAAVNPDA
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHCCCCCEEEEEEEEECCCH
ALFSSDFRAPERQFSLLMQAAGRAGRAAEQADRAEMWVQTWHPQHALYAALRTHDYAAFA
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QAQLAERRAAGLPPYTHLALLRADAREQSVAQAWLRVVSDEARELAEQHQVALYPPVPLS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCH
VQRVADMERAQLLVESASRPALQRFLTAWDATLRRKRPEQKGLLRWAVDVDPLAI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCC
>Mature Secondary Structure 
GKSPSSRAAATSAPAAGAEPPAPAREPVVVPVVIDAPQYSGLKAALGYLSERPLTPGTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCE
LRVPLGRREVLGVAWDTRSGEDSAVPDGVTLREVAAVFDALPPLPAAWRELVDFAASYYQ
EECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RSPGEVALAVLPPELRMLSATQLERRLTRLRAKLLPPSGAQGALPAPSAEQAQVLQALAA
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
SAGDAPVTLLHGSTGSGKTEIYLREAQRVVEAGRQALVLVPEINLTPQLEERFLARFPGR
CCCCCCEEEEECCCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCC
RVVSLHSGLTPARRLKHWLMVLLGEADLVLGTRLAVFAPLPRLGLVIVDEEHDPSYKQQE
EEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHC
GARYSARDLAVVRGRVEGVPVLLGSATPSLESWHNALPATEGGAGRYTRLTMAARIGGGV
CCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCCCCCCCCCCEEEHHHHHCCCC
LPTVRLVDMSRLPKEVLRDSALSPALLGALRERLARGEQSLVLLNRRGYAPVLHCTECGW
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEECCCCH
KSACAHCSAWRVFHKLDRTLRCHHCGLTERVPRACPDCGNADIQPTGRGTERLEEQLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
LPGARVGRIDADTTRRSGALEAELSRVHAGEVDVLVGTQMIAKGHDFRRMTLVAAVNPDA
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECHHHHHCCCCCEEEEEEEEECCCH
ALFSSDFRAPERQFSLLMQAAGRAGRAAEQADRAEMWVQTWHPQHALYAALRTHDYAAFA
HHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QAQLAERRAAGLPPYTHLALLRADAREQSVAQAWLRVVSDEARELAEQHQVALYPPVPLS
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCH
VQRVADMERAQLLVESASRPALQRFLTAWDATLRRKRPEQKGLLRWAVDVDPLAI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]