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The map label for this gene is pyrE

Identifier: 124265281

GI number: 124265281

Start: 84496

End: 85200

Strand: Reverse

Name: pyrE

Synonym: Mpe_A0088

Alternate gene names: 124265281

Gene position: 85200-84496 (Counterclockwise)

Preceding gene: 124265286

Following gene: 124265280

Centisome position: 2.11

GC content: 67.94

Gene sequence:

>705_bases
ATGAGCACATTCGGCGGCCCGGGCACTGCGGCGCGAGACGACCTCGCACAGGACTTCGTGGCCTTCGCGGTCGAGGCCGG
CGTGCTGCGCTTCGGCGAATTCAAGACCAAGGCCGGGCGCCTGTCTCCGTATTTCTTCAACGCCGGGCTGTTCGACGACG
GCGCCAAGCTGGGCCGCCTCGCCGAATTCTATGCACGCCGGATGCAGGCTTCCGGCCTGGCTTTCGACATGATCTTCGGC
CCGGCCTACAAGGGCATCACGCTGGCCGCCGCGGTGGCGATCGAGCTCGCCCGGCTCGGCCGCAATCTGCCGTATGCCTA
CAACCGCAAGGAGACCAAGGACCACGGCGAGGGCGGCAGCCTTGTCGGCGCCAAGCTGCACGGCCGCGTTGTGATCATCG
ACGACGTGATTTCGGCCGGCACCTCGGTGCGCGAATCGGTCGCGATGATCCGGGCCGCCGGGGCCGAACCCTGCGGCGTG
GCCATCGCGCTCGACCGCCAGGAGCGGGCCACCGAAACCACTCAAGACGGAGTGCGCGATGCCGATCATTCCGCGGTGCA
GTTCGTGCAGCGTGAACTCGGCCTGGCGGTCTGTGCGATTGCGACGCTGTCGGACTTGCTGCAGTATCTGGCGCGCCAGG
CTGACCCGGCGCTGGCACAACACCTGCCACGGGTGCAGGCCTACCGGGATCGCTACGGCGTCTGA

Upstream 100 bases:

>100_bases
GGCCCATTCGACCCAGCCCTTGTTGGCCGCGGAACGGATGCCGTTGAGATTGAGCGAGACCAGACGCAACACGACGCACC
TACCTATTTCAGATGTCCAG

Downstream 100 bases:

>100_bases
GACATGAAGGAACGGGGAGCGGGCGGGATGAGGATCTGTCGTGTGCGCGGACTGAACGCCGCCCTGATGCTCGGCGTGCT
GGGGTCGCTGGCGGTGGACG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSTFGGPGTAARDDLAQDFVAFAVEAGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRLAEFYARRMQASGLAFDMIFG
PAYKGITLAAAVAIELARLGRNLPYAYNRKETKDHGEGGSLVGAKLHGRVVIIDDVISAGTSVRESVAMIRAAGAEPCGV
AIALDRQERATETTQDGVRDADHSAVQFVQRELGLAVCAIATLSDLLQYLARQADPALAQHLPRVQAYRDRYGV

Sequences:

>Translated_234_residues
MSTFGGPGTAARDDLAQDFVAFAVEAGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRLAEFYARRMQASGLAFDMIFG
PAYKGITLAAAVAIELARLGRNLPYAYNRKETKDHGEGGSLVGAKLHGRVVIIDDVISAGTSVRESVAMIRAAGAEPCGV
AIALDRQERATETTQDGVRDADHSAVQFVQRELGLAVCAIATLSDLLQYLARQADPALAQHLPRVQAYRDRYGV
>Mature_233_residues
STFGGPGTAARDDLAQDFVAFAVEAGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRLAEFYARRMQASGLAFDMIFGP
AYKGITLAAAVAIELARLGRNLPYAYNRKETKDHGEGGSLVGAKLHGRVVIIDDVISAGTSVRESVAMIRAAGAEPCGVA
IALDRQERATETTQDGVRDADHSAVQFVQRELGLAVCAIATLSDLLQYLARQADPALAQHLPRVQAYRDRYGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=186, Percent_Identity=26.3440860215054, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1790073, Length=219, Percent_Identity=50.6849315068493, Blast_Score=216, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17508631, Length=156, Percent_Identity=29.4871794871795, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323530, Length=226, Percent_Identity=44.2477876106195, Blast_Score=186, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323927, Length=231, Percent_Identity=41.1255411255411, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METPP (A2SBW1)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019285.1
- ProteinModelPortal:   A2SBW1
- SMR:   A2SBW1
- STRING:   A2SBW1
- GeneID:   4783874
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0088
- NMPDR:   fig|279263.3.peg.4271
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   A2SBW1
- ProtClustDB:   PRK00455
- BioCyc:   MPET420662:MPE_A0088-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 25085; Mature: 24954

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 37-37 BINDING 109-109 BINDING 110-110 BINDING 113-113 BINDING 115-115 BINDING 138-138 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFGGPGTAARDDLAQDFVAFAVEAGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHH
AEFYARRMQASGLAFDMIFGPAYKGITLAAAVAIELARLGRNLPYAYNRKETKDHGEGGS
HHHHHHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LVGAKLHGRVVIIDDVISAGTSVRESVAMIRAAGAEPCGVAIALDRQERATETTQDGVRD
EEEEEECCCEEEEECHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCC
ADHSAVQFVQRELGLAVCAIATLSDLLQYLARQADPALAQHLPRVQAYRDRYGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure 
STFGGPGTAARDDLAQDFVAFAVEAGVLRFGEFKTKAGRLSPYFFNAGLFDDGAKLGRL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHH
AEFYARRMQASGLAFDMIFGPAYKGITLAAAVAIELARLGRNLPYAYNRKETKDHGEGGS
HHHHHHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
LVGAKLHGRVVIIDDVISAGTSVRESVAMIRAAGAEPCGVAIALDRQERATETTQDGVRD
EEEEEECCCEEEEECHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCC
ADHSAVQFVQRELGLAVCAIATLSDLLQYLARQADPALAQHLPRVQAYRDRYGV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA