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The map label for this gene is rfcL

Identifier: 124027779

GI number: 124027779

Start: 913356

End: 914810

Strand: Direct

Name: rfcL

Synonym: Hbut_0906

Alternate gene names: 124027779

Gene position: 913356-914810 (Clockwise)

Preceding gene: 124027776

Following gene: 124027785

Centisome position: 54.79

GC content: 47.01

Gene sequence:

>1455_bases
ATGAGCGATAAGCTTCCATGGATTATAAAGTATAGGCCGAAGAGGGTTGAGGATGTTGTAGACCAGGAGGAGGCTAAGAA
GCTGTTTTTACCGTGGCTTAGGAGCTGGCTACAGGGACGTATCCCGGAGAGGAAGGCAGCCCTCTTCTACGGCCCAGCAG
GTGTCGGTAAGACTAGTCTTGTTGAAGCTGCTGCTAACGAATATGGGCTAGAGCTTATCGAGATGAATGCATCTGATTTT
AGGCGTAAGAGCGATATTGAGCGTATAGCTAAGATTGCTGCTACACAGTTTAGCCTCTTTGGCAGAAAGCGTAAGATAAT
CCTTCTAGACGAGGTTGATGGTATTTCGGGTACAGCTGACAGGGGTGGATTGGATGCCATATTGGAGCTGATAAACATTA
CTAAGCATCCAATAGTTATGACTGCTAATGATCCATGGGATCAGAAGCTTAAGCCTCTACGTGATGCATCGCTTATGGTG
CCCTTCTATAGGCTTTCAGAGCGCTATGTAGTGCAAGTTCTCAAGCGAATATGTGCTGCAGAGAATATTGAGTGTGAAGA
TGAGGCTCTTAAGCTTATTGCACAGCGTGCTGAGGGGGATCTAAGATCGGCGATAAATGATCTACAAGCTATCGCTGAGG
GCTATGGTGTAGTCCGAGTTGACCTTGTCAGGGCGCTTCTCGCTACCAGGGATAGGCAGTACTCGCCGTGGGAGATGCTG
CGAAACCTCTTCATGTCAAAATATATATGGCAGGCTAAACGTGCAGTAACACATACAGACCTAGACTATGATACACTGCT
CCAGTGGATTAATGAGAACATAGCTGTCCAGTATACACATCCAGAGGATATTTGGCGTGCACACGAGGCGCTTGCTAGGG
CCGACATTTTCCTCGGACGTATTAAGAGGAGCCTATCCTGGGACCTGCTTCCATATGTCTTTGATCTTGTTGGGCCTGGC
GTAGCACTAGCTAGGAAGAAGAGTAAGTTTAAGTGGGCAAAGTACCAATTCCCTCAGAAAATACTAATGCTTGCTAAGAC
GAAGGAGGTTAGAGAGATTCGTGAAGCTGTGGCAGAGGTATTTGCCAAGAGGCTGCATACATCTAAGGCTACTGTAAAGC
GTGAAGTCCTACCCTTCCTGTTCATAATCTTCCGTGAAAGGCCAGACTATGCTGCAAGAATAGCTATAGGCTACAATCTT
ACGGATAACATGATAAAGTACCTAGCTGGCCCCCTTGCTTCAAAGGTTAAGGAGCATATGAATGCCCTACTTATGAGGTT
GGAAAAGCCTGCGACAGTATCCACAGCTACAACTGCGACTACAGCACGAACCACAGCTGCAGAAACTTCAGTGGCAGCTT
CGAGAGCGGCTAAGAGAACTAGTACATCTCGCCGTAGAACTAGTAAGTCTAGAAGGAGAGGTAAGGGTAGCGGTACTCAA
ACAACACTATTCTAG

Upstream 100 bases:

>100_bases
ACGAGATCCAGTTGACAGCGTTCCTTGCATGGCTAACGCTGCTAGGGCAGAAACTGAAGGGCGGCAGTTAGCATTTCTAC
GGCCTGGGGAGTGAAGGTTT

Downstream 100 bases:

>100_bases
ACTCCTTTTATGAGCCGTGTTTGACTTTGCTTGTAACCTTTACCTCTTCTATAACAGTGTGCGGGTATAGCCCACGCTCA
TCTTTCTCGTACTTCTTAAC

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MSDKLPWIIKYRPKRVEDVVDQEEAKKLFLPWLRSWLQGRIPERKAALFYGPAGVGKTSLVEAAANEYGLELIEMNASDF
RRKSDIERIAKIAATQFSLFGRKRKIILLDEVDGISGTADRGGLDAILELINITKHPIVMTANDPWDQKLKPLRDASLMV
PFYRLSERYVVQVLKRICAAENIECEDEALKLIAQRAEGDLRSAINDLQAIAEGYGVVRVDLVRALLATRDRQYSPWEML
RNLFMSKYIWQAKRAVTHTDLDYDTLLQWINENIAVQYTHPEDIWRAHEALARADIFLGRIKRSLSWDLLPYVFDLVGPG
VALARKKSKFKWAKYQFPQKILMLAKTKEVREIREAVAEVFAKRLHTSKATVKREVLPFLFIIFRERPDYAARIAIGYNL
TDNMIKYLAGPLASKVKEHMNALLMRLEKPATVSTATTATTARTTAAETSVAASRAAKRTSTSRRRTSKSRRRGKGSGTQ
TTLF

Sequences:

>Translated_484_residues
MSDKLPWIIKYRPKRVEDVVDQEEAKKLFLPWLRSWLQGRIPERKAALFYGPAGVGKTSLVEAAANEYGLELIEMNASDF
RRKSDIERIAKIAATQFSLFGRKRKIILLDEVDGISGTADRGGLDAILELINITKHPIVMTANDPWDQKLKPLRDASLMV
PFYRLSERYVVQVLKRICAAENIECEDEALKLIAQRAEGDLRSAINDLQAIAEGYGVVRVDLVRALLATRDRQYSPWEML
RNLFMSKYIWQAKRAVTHTDLDYDTLLQWINENIAVQYTHPEDIWRAHEALARADIFLGRIKRSLSWDLLPYVFDLVGPG
VALARKKSKFKWAKYQFPQKILMLAKTKEVREIREAVAEVFAKRLHTSKATVKREVLPFLFIIFRERPDYAARIAIGYNL
TDNMIKYLAGPLASKVKEHMNALLMRLEKPATVSTATTATTARTTAAETSVAASRAAKRTSTSRRRTSKSRRRGKGSGTQ
TTLF
>Mature_483_residues
SDKLPWIIKYRPKRVEDVVDQEEAKKLFLPWLRSWLQGRIPERKAALFYGPAGVGKTSLVEAAANEYGLELIEMNASDFR
RKSDIERIAKIAATQFSLFGRKRKIILLDEVDGISGTADRGGLDAILELINITKHPIVMTANDPWDQKLKPLRDASLMVP
FYRLSERYVVQVLKRICAAENIECEDEALKLIAQRAEGDLRSAINDLQAIAEGYGVVRVDLVRALLATRDRQYSPWEMLR
NLFMSKYIWQAKRAVTHTDLDYDTLLQWINENIAVQYTHPEDIWRAHEALARADIFLGRIKRSLSWDLLPYVFDLVGPGV
ALARKKSKFKWAKYQFPQKILMLAKTKEVREIREAVAEVFAKRLHTSKATVKREVLPFLFIIFRERPDYAARIAIGYNLT
DNMIKYLAGPLASKVKEHMNALLMRLEKPATVSTATTATTARTTAAETSVAASRAAKRTSTSRRRTSKSRRRGKGSGTQT
TLF

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily

Homologues:

Organism=Homo sapiens, GI32528306, Length=432, Percent_Identity=25.6944444444444, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4506491, Length=222, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI31881687, Length=222, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI6677723, Length=225, Percent_Identity=28, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI31563534, Length=227, Percent_Identity=26.8722466960352, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194306571, Length=219, Percent_Identity=27.3972602739726, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI194306567, Length=219, Percent_Identity=27.3972602739726, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI27501458, Length=198, Percent_Identity=25.7575757575758, Blast_Score=72, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17563226, Length=349, Percent_Identity=27.2206303724928, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17541988, Length=221, Percent_Identity=30.7692307692308, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17541368, Length=244, Percent_Identity=25.4098360655738, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17554730, Length=222, Percent_Identity=32.4324324324324, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71986063, Length=256, Percent_Identity=26.5625, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324791, Length=352, Percent_Identity=24.7159090909091, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324478, Length=231, Percent_Identity=31.1688311688312, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322528, Length=321, Percent_Identity=25.2336448598131, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324039, Length=217, Percent_Identity=29.4930875576037, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17737765, Length=359, Percent_Identity=25.0696378830084, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI62472391, Length=381, Percent_Identity=24.4094488188976, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574716, Length=371, Percent_Identity=23.989218328841, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI18859927, Length=218, Percent_Identity=30.7339449541284, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17647857, Length=225, Percent_Identity=28.4444444444444, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19921076, Length=238, Percent_Identity=26.890756302521, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCL_HYPBU (A2BL93)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013099.1
- ProteinModelPortal:   A2BL93
- STRING:   A2BL93
- GeneID:   4782052
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0906
- eggNOG:   arNOG04036
- HOGENOM:   HBG541322
- OMA:   ELNASDK
- PhylomeDB:   A2BL93
- ProtClustDB:   PRK04195
- BioCyc:   HBUT415426:HBUT_0906-MONOMER
- HAMAP:   MF_01508
- InterPro:   IPR003593
- InterPro:   IPR003959
- SMART:   SM00382

Pfam domain/function: PF00004 AAA

EC number: NA

Molecular weight: Translated: 55157; Mature: 55025

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKLPWIIKYRPKRVEDVVDQEEAKKLFLPWLRSWLQGRIPERKAALFYGPAGVGKTSL
CCCCCCEEEEECCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHCEEEECCCCCCHHHH
VEAAANEYGLELIEMNASDFRRKSDIERIAKIAATQFSLFGRKRKIILLDEVDGISGTAD
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
RGGLDAILELINITKHPIVMTANDPWDQKLKPLRDASLMVPFYRLSERYVVQVLKRICAA
CCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
ENIECEDEALKLIAQRAEGDLRSAINDLQAIAEGYGVVRVDLVRALLATRDRQYSPWEML
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
RNLFMSKYIWQAKRAVTHTDLDYDTLLQWINENIAVQYTHPEDIWRAHEALARADIFLGR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
IKRSLSWDLLPYVFDLVGPGVALARKKSKFKWAKYQFPQKILMLAKTKEVREIREAVAEV
HHHHHCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
FAKRLHTSKATVKREVLPFLFIIFRERPDYAARIAIGYNLTDNMIKYLAGPLASKVKEHM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
NALLMRLEKPATVSTATTATTARTTAAETSVAASRAAKRTSTSRRRTSKSRRRGKGSGTQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TTLF
CCCC
>Mature Secondary Structure 
SDKLPWIIKYRPKRVEDVVDQEEAKKLFLPWLRSWLQGRIPERKAALFYGPAGVGKTSL
CCCCCEEEEECCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHCEEEECCCCCCHHHH
VEAAANEYGLELIEMNASDFRRKSDIERIAKIAATQFSLFGRKRKIILLDEVDGISGTAD
HHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
RGGLDAILELINITKHPIVMTANDPWDQKLKPLRDASLMVPFYRLSERYVVQVLKRICAA
CCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
ENIECEDEALKLIAQRAEGDLRSAINDLQAIAEGYGVVRVDLVRALLATRDRQYSPWEML
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHH
RNLFMSKYIWQAKRAVTHTDLDYDTLLQWINENIAVQYTHPEDIWRAHEALARADIFLGR
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
IKRSLSWDLLPYVFDLVGPGVALARKKSKFKWAKYQFPQKILMLAKTKEVREIREAVAEV
HHHHHCHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
FAKRLHTSKATVKREVLPFLFIIFRERPDYAARIAIGYNLTDNMIKYLAGPLASKVKEHM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHH
NALLMRLEKPATVSTATTATTARTTAAETSVAASRAAKRTSTSRRRTSKSRRRGKGSGTQ
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
TTLF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA