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The map label for this gene is thiI [C]

Identifier: 124027318

GI number: 124027318

Start: 416207

End: 417298

Strand: Reverse

Name: thiI [C]

Synonym: Hbut_0427

Alternate gene names: 124027318

Gene position: 417298-416207 (Counterclockwise)

Preceding gene: 124027319

Following gene: 124027316

Centisome position: 25.03

GC content: 53.39

Gene sequence:

>1092_bases
TTGTCTAGCGGCGCTGGCGGCCGGTGTGACTACATTCTTGTAAAAACTGTGCTTGGTATGGAGCGCGTAGCAGCGAGCTA
TATAGCCGAGATTGATCCGGGTGCAGAGGTTATACCTGCGCCGCGTGGGTTTAAGGGCCTGGTTCTGGTTAGGCCTTCCC
GCGACAAGTACAAGGTGGCTGAGGAGTTAGAGAAGCGTATTCCAGAGGCAGAGAAGATCGTCGTCATTGAAGGGTGTGCG
CCTGCGGATCCCCGTGAGATTGCTAGGGTTGCAGCGGAGCTAGCGCCGCAGTACATCTCTAGCAATGAAACCTTTGCTGT
TCGGACTGTTAGGCGTGGGCGCCACAACTTTACAAGCATAGACGTAAACGTGGTCGTGGGCGATGCTGTTAGGAGGGCTA
CTGGTGCACGGGTAAACCTGAGGTTTCCCGACAAGGTTGTTGCTGTTGAGATAATTGCTGACCGGGCATTCATAGCCTTC
TATCCCGGCTCCAAGGAGTGGAAGAAGATGCGGCCAGGCAAGTATCCCGTCTACAAGCTGCTGCGCCGCATAGCTATAGT
GCAGATGCCTTACCTAGGCCCTCTCGATGCTTGCAAGACGATGGGTGTGAGGATAGGTAGAGAGGTGCAGAACTTCGAGG
TGGGAGAACTCGTCATAGCTCCTATAGGGCTTGTTGATGCGCTCCAGCTCAAAACATTCCTTGAAGGAGTTTTCGAGGGT
ATCGAGTCTAGGTACCAGGTGCAAGTGAAGAGCTATGGTAGACAAGTATACAAGGTCCCCGTCTATGTACAGGATCTATA
CCAGCTTGTTAGGAGCCGCTTCAACGAACCCATAATAGTCTTCGAGCCTGAGGGAGATCCGGTCTCGAAGCGGGCCAAGG
ACCTCTACGAGCTTCTCCGTAGTGGCCGTCGGGTAAACATGTTGTTTGGGTCTAGGGAGGGCATACCTGAGGGTGTATAC
CGCTTTGCAAACCTCGTACTAGACTTTGCGCCGGGCGTAACCCTCTCGACAGAATACGCTGCTGCTGCGGCAGTCATAGC
TGTCGTAAGTGTTGTCCATGACTATTTTGCCTCTGAGGAGGAAGAATCTTAG

Upstream 100 bases:

>100_bases
TTTCCCATTACAAGCCTACTCCTAAATTCCTCAGCTATTCAATGCTGAGTTCTTTCCCTGGCATACAATTCCCAGGCCTT
AGCTGTGGGTGGGTTGGGTA

Downstream 100 bases:

>100_bases
ACGCTTACACCTCTCCTTTTCAGCTCCATGGATAGCTGCCTGGCTAGTATGGGGTCCTGCCGTCCTAGCTCTCTCAAGAG
CCTCCTAAGACTCTGCAATG

Product: RNA-binding protein

Products: ThiS-COSH; AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSSGAGGRCDYILVKTVLGMERVAASYIAEIDPGAEVIPAPRGFKGLVLVRPSRDKYKVAEELEKRIPEAEKIVVIEGCA
PADPREIARVAAELAPQYISSNETFAVRTVRRGRHNFTSIDVNVVVGDAVRRATGARVNLRFPDKVVAVEIIADRAFIAF
YPGSKEWKKMRPGKYPVYKLLRRIAIVQMPYLGPLDACKTMGVRIGREVQNFEVGELVIAPIGLVDALQLKTFLEGVFEG
IESRYQVQVKSYGRQVYKVPVYVQDLYQLVRSRFNEPIIVFEPEGDPVSKRAKDLYELLRSGRRVNMLFGSREGIPEGVY
RFANLVLDFAPGVTLSTEYAAAAAVIAVVSVVHDYFASEEEES

Sequences:

>Translated_363_residues
MSSGAGGRCDYILVKTVLGMERVAASYIAEIDPGAEVIPAPRGFKGLVLVRPSRDKYKVAEELEKRIPEAEKIVVIEGCA
PADPREIARVAAELAPQYISSNETFAVRTVRRGRHNFTSIDVNVVVGDAVRRATGARVNLRFPDKVVAVEIIADRAFIAF
YPGSKEWKKMRPGKYPVYKLLRRIAIVQMPYLGPLDACKTMGVRIGREVQNFEVGELVIAPIGLVDALQLKTFLEGVFEG
IESRYQVQVKSYGRQVYKVPVYVQDLYQLVRSRFNEPIIVFEPEGDPVSKRAKDLYELLRSGRRVNMLFGSREGIPEGVY
RFANLVLDFAPGVTLSTEYAAAAAVIAVVSVVHDYFASEEEES
>Mature_362_residues
SSGAGGRCDYILVKTVLGMERVAASYIAEIDPGAEVIPAPRGFKGLVLVRPSRDKYKVAEELEKRIPEAEKIVVIEGCAP
ADPREIARVAAELAPQYISSNETFAVRTVRRGRHNFTSIDVNVVVGDAVRRATGARVNLRFPDKVVAVEIIADRAFIAFY
PGSKEWKKMRPGKYPVYKLLRRIAIVQMPYLGPLDACKTMGVRIGREVQNFEVGELVIAPIGLVDALQLKTFLEGVFEGI
ESRYQVQVKSYGRQVYKVPVYVQDLYQLVRSRFNEPIIVFEPEGDPVSKRAKDLYELLRSGRRVNMLFGSREGIPEGVYR
FANLVLDFAPGVTLSTEYAAAAAVIAVVSVVHDYFASEEEES

Specific function: Required For The Synthesis Of The Thiazole Moiety. [C]

COG id: COG1818

COG function: function code R; Predicted RNA-binding protein, contains THUMP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004114 [H]

Pfam domain/function: PF02926 THUMP [H]

EC number: NA

Molecular weight: Translated: 40364; Mature: 40233

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGAGGRCDYILVKTVLGMERVAASYIAEIDPGAEVIPAPRGFKGLVLVRPSRDKYKVA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
EELEKRIPEAEKIVVIEGCAPADPREIARVAAELAPQYISSNETFAVRTVRRGRHNFTSI
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCEEE
DVNVVVGDAVRRATGARVNLRFPDKVVAVEIIADRAFIAFYPGSKEWKKMRPGKYPVYKL
EEEEEECHHHHHHCCCEEEEECCCCEEEEEEEECCEEEEECCCCHHHHHCCCCCCHHHHH
LRRIAIVQMPYLGPLDACKTMGVRIGREVQNFEVGELVIAPIGLVDALQLKTFLEGVFEG
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
IESRYQVQVKSYGRQVYKVPVYVQDLYQLVRSRFNEPIIVFEPEGDPVSKRAKDLYELLR
HHHHEEEEHHHHCCEEEEECHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
SGRRVNMLFGSREGIPEGVYRFANLVLDFAPGVTLSTEYAAAAAVIAVVSVVHDYFASEE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCC
EES
CCC
>Mature Secondary Structure 
SSGAGGRCDYILVKTVLGMERVAASYIAEIDPGAEVIPAPRGFKGLVLVRPSRDKYKVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
EELEKRIPEAEKIVVIEGCAPADPREIARVAAELAPQYISSNETFAVRTVRRGRHNFTSI
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCEEE
DVNVVVGDAVRRATGARVNLRFPDKVVAVEIIADRAFIAFYPGSKEWKKMRPGKYPVYKL
EEEEEECHHHHHHCCCEEEEECCCCEEEEEEEECCEEEEECCCCHHHHHCCCCCCHHHHH
LRRIAIVQMPYLGPLDACKTMGVRIGREVQNFEVGELVIAPIGLVDALQLKTFLEGVFEG
HHHHHHHHCCCCCCHHHHHHHHHHHCCHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHH
IESRYQVQVKSYGRQVYKVPVYVQDLYQLVRSRFNEPIIVFEPEGDPVSKRAKDLYELLR
HHHHEEEEHHHHCCEEEEECHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHH
SGRRVNMLFGSREGIPEGVYRFANLVLDFAPGVTLSTEYAAAAAVIAVVSVVHDYFASEE
CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCC
EES
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]