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The map label for this gene is pyrI

Identifier: 124027009

GI number: 124027009

Start: 98029

End: 98511

Strand: Reverse

Name: pyrI

Synonym: Hbut_0109

Alternate gene names: 124027009

Gene position: 98511-98029 (Counterclockwise)

Preceding gene: 124027010

Following gene: 124027008

Centisome position: 5.91

GC content: 54.24

Gene sequence:

>483_bases
TTGGAGAGCGGGTCAGGCCTCCTGGTTAGGAGGATACGTGAGGGAACCGTGATAGACCATATACCTGCAGGTAGGGCTTT
GACTGTGTTAAAGATACTGGGCATAACTGGGCGCGAGGGAGCTAGGATAGCTGTTGTGATGAATGTTGAGAGTCGGAAGC
TTGGCCGGAAAGATATCGTGAAGATTGAGGGTCGCCACCTTAGCCCCGAGGAGGTGGATAAGATAGCATTGGTAGCTCCG
AGGGCTACAATAAACATTATCCATGACTACCGTGTCGTGGCGAAGCGTCGTGTCACAGTGCCAGACATTGTTGAGGGTAT
ACTGATGTGCCCCAATCCGTCCTGCATAACGAGGGTTGGGAGGGAGCCGGTGAAGCCGAGATTCCGTGTAGTGTCGAGGC
AGCCACTGGTGCTCCAGTGCGTCTATTGTGGTACCCTCGTATCGGAGGAGGATGTGGCCGAGCAATTAGCTGAGGGAGGC
TAG

Upstream 100 bases:

>100_bases
GACGGGACCCCATACGCTGCCTACTTTGAACAAGCCCGTAACGGTGTGCCAGTCCGTATGGCGCTCCTAGCTCTCATAGC
TGGCCGGGAGGTGTAGCCGG

Downstream 100 bases:

>100_bases
GTGTGCAACGCTACAGCTACCATAGCGTTGAGCTTGTGCGCATACTATGGCGAGGTCCGCGTCAGGTTATGCTAGAGTAT
CGCTACCTAGACAGTGCCCC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MESGSGLLVRRIREGTVIDHIPAGRALTVLKILGITGREGARIAVVMNVESRKLGRKDIVKIEGRHLSPEEVDKIALVAP
RATINIIHDYRVVAKRRVTVPDIVEGILMCPNPSCITRVGREPVKPRFRVVSRQPLVLQCVYCGTLVSEEDVAEQLAEGG

Sequences:

>Translated_160_residues
MESGSGLLVRRIREGTVIDHIPAGRALTVLKILGITGREGARIAVVMNVESRKLGRKDIVKIEGRHLSPEEVDKIALVAP
RATINIIHDYRVVAKRRVTVPDIVEGILMCPNPSCITRVGREPVKPRFRVVSRQPLVLQCVYCGTLVSEEDVAEQLAEGG
>Mature_160_residues
MESGSGLLVRRIREGTVIDHIPAGRALTVLKILGITGREGARIAVVMNVESRKLGRKDIVKIEGRHLSPEEVDKIALVAP
RATINIIHDYRVVAKRRVTVPDIVEGILMCPNPSCITRVGREPVKPRFRVVSRQPLVLQCVYCGTLVSEEDVAEQLAEGG

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=144, Percent_Identity=41.6666666666667, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_HYPBU (A2BJ23)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001012329.1
- ProteinModelPortal:   A2BJ23
- SMR:   A2BJ23
- STRING:   A2BJ23
- GeneID:   4781406
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0109
- eggNOG:   arNOG10217
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- PhylomeDB:   A2BJ23
- ProtClustDB:   PRK00893
- BioCyc:   HBUT415426:HBUT_0109-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17564; Mature: 17564

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESGSGLLVRRIREGTVIDHIPAGRALTVLKILGITGREGARIAVVMNVESRKLGRKDIV
CCCCCCCEEEEHHCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCHHHCCCCCEE
KIEGRHLSPEEVDKIALVAPRATINIIHDYRVVAKRRVTVPDIVEGILMCPNPSCITRVG
EECCCCCCHHHHCEEEEECCCCEEEEEHHHHHHHHCCCCCHHHHCCEEECCCCHHHHHCC
REPVKPRFRVVSRQPLVLQCVYCGTLVSEEDVAEQLAEGG
CCCCCCHHEEECCCCCEEEEEECCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MESGSGLLVRRIREGTVIDHIPAGRALTVLKILGITGREGARIAVVMNVESRKLGRKDIV
CCCCCCCEEEEHHCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCHHHCCCCCEE
KIEGRHLSPEEVDKIALVAPRATINIIHDYRVVAKRRVTVPDIVEGILMCPNPSCITRVG
EECCCCCCHHHHCEEEEECCCCEEEEEHHHHHHHHCCCCCHHHHCCEEECCCCHHHHHCC
REPVKPRFRVVSRQPLVLQCVYCGTLVSEEDVAEQLAEGG
CCCCCCHHEEECCCCCEEEEEECCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA