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The map label for this gene is kefB [H]

Identifier: 124026755

GI number: 124026755

Start: 1693553

End: 1694926

Strand: Reverse

Name: kefB [H]

Synonym: NATL1_20501

Alternate gene names: 124026755

Gene position: 1694926-1693553 (Counterclockwise)

Preceding gene: 124026757

Following gene: 124026754

Centisome position: 90.89

GC content: 41.05

Gene sequence:

>1374_bases
ATGGTATTAACTCCTTTAGTCTCAGCACTTAATACTCATGATGTTGAGGTAGCGGAAACGCTGATAGGGGTCATTAATTT
CTTGATGATCTTTGTTGCGGCAAGGACTTTGGCTGAAATCTTGGTTCGACTAAGTTTGCCTACAATAGTCGGTGAACTTC
TTGCGGGAGTTTTAATTGGTGCCTCAGGATTACATCTTTTATTGCCGCCTAGTGCTCATGCTGAGTTGAATCAAGGTTTT
GTTTCTGTTATTAGCACACTTGCTTCAGTACCCAAAGAAGCAGTCCCAGATATTTATTTTGAAACTTTTCCATCATTACA
AGCCGTAGCAACTTTAGGGCTATACGCTTTGTTATTTCTGACCGGACTGGAGAGTGAATTAGAGGAACTTGTAGCAGTTG
GAGCACAAGCTTTCACTGTTGCAATGGCAGGAGTGATTTTGCCATTTGCTTTTGGAACTTTTGGATTAATGTTTATTTTC
CAAGTAGATATCATTCCTGCGATATTTGCTGGTGCATCTATGACCGCGACAAGTATTGGTATTACTGCAAGTGTTTTTGG
TGAGCTTGGTTATTTAAAAACTCGTGAGGGTCAAATTGTTATTGGCGCAGCAGTATTGGATGACATACTTGGAATTGTTA
TTCTTGCCGTTGTTGTAGCTCTTGCGACGGGAGGATCATTACAAATTGCTCCCATTGTTAAATTAGTACTTGCTGCAACT
GTTTTTGTGTTTGCTGCAATTGCTTTAAGTCGAACAGCTGCTCCAGCCTTTGATTGGTTGCTTGAGAGACTTAAAGCTCC
CGGAGCGGTTGTTGTAGCTTCTTTTGTGATACTAGTCTTGAGCTGTTTTGTTGCTACTGCTATAGGTTTAGAAGCTGCTT
TGGGTGCTTTTGCTGCTGGATTGATACTAAGTAGTTCAAAAAATAATCACGCAATTCAACAATCTGTTTTGCCATTAGTA
TCGCTCTTCGCAACTATCTTCTTTGTACTTGTTGGAGCAGGTATGGATCTTTCTGTGATCAATCCTCTTGATCCACAGAG
TCGCTCTGCTCTAATCGTGGCTGGTTTTTTATTTATTGTGGCAATTGTCGGGAAAATCGCTGCTGGCTGGTGCTTTGTCA
TTGATAAACCAACAAATAGATTAGTAGTTGGCCTGGGAATGATGCCTCGAGGTGAGGTCGGATTGATTTTTCTAGGTTTG
GGAACAAGTGCTGGTTTGCTTACCCCTTCTCTTGAGGCTGCAATATTGTTAATGGTAATTGGAACAACATTTTTAGCACC
TGTTTTGCTTAGAGTTGTTTTGAAAGATAAACCTCCTTCAGGAGGCAACTCTATTCCTGATGAGATTGCAGCTGATCCAG
TTGGATTGGTTTAG

Upstream 100 bases:

>100_bases
TCCACACCTTATTGATTAAGTCACCTTTGATACTTATGGCAAATATGCCCTGTGATTAGGTAATTTATGTCTTAAAGAAA
AGGCTTTTCTAGAAAACCTG

Downstream 100 bases:

>100_bases
AAATCATCAAAAACTCTTTTTCAAAAAGGTGAGGCAATTTCGAAAAAAAGAAATAAAATCATTAATCTTTTTCTATCAAT
TATTTACTAAGATCTAGAAG

Product: CPA2 family Na+/H+ antiporter

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MVLTPLVSALNTHDVEVAETLIGVINFLMIFVAARTLAEILVRLSLPTIVGELLAGVLIGASGLHLLLPPSAHAELNQGF
VSVISTLASVPKEAVPDIYFETFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIF
QVDIIPAIFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLQIAPIVKLVLAAT
VFVFAAIALSRTAAPAFDWLLERLKAPGAVVVASFVILVLSCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLV
SLFATIFFVLVGAGMDLSVINPLDPQSRSALIVAGFLFIVAIVGKIAAGWCFVIDKPTNRLVVGLGMMPRGEVGLIFLGL
GTSAGLLTPSLEAAILLMVIGTTFLAPVLLRVVLKDKPPSGGNSIPDEIAADPVGLV

Sequences:

>Translated_457_residues
MVLTPLVSALNTHDVEVAETLIGVINFLMIFVAARTLAEILVRLSLPTIVGELLAGVLIGASGLHLLLPPSAHAELNQGF
VSVISTLASVPKEAVPDIYFETFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIF
QVDIIPAIFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLQIAPIVKLVLAAT
VFVFAAIALSRTAAPAFDWLLERLKAPGAVVVASFVILVLSCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLV
SLFATIFFVLVGAGMDLSVINPLDPQSRSALIVAGFLFIVAIVGKIAAGWCFVIDKPTNRLVVGLGMMPRGEVGLIFLGL
GTSAGLLTPSLEAAILLMVIGTTFLAPVLLRVVLKDKPPSGGNSIPDEIAADPVGLV
>Mature_457_residues
MVLTPLVSALNTHDVEVAETLIGVINFLMIFVAARTLAEILVRLSLPTIVGELLAGVLIGASGLHLLLPPSAHAELNQGF
VSVISTLASVPKEAVPDIYFETFPSLQAVATLGLYALLFLTGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIF
QVDIIPAIFAGASMTATSIGITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLQIAPIVKLVLAAT
VFVFAAIALSRTAAPAFDWLLERLKAPGAVVVASFVILVLSCFVATAIGLEAALGAFAAGLILSSSKNNHAIQQSVLPLV
SLFATIFFVLVGAGMDLSVINPLDPQSRSALIVAGFLFIVAIVGKIAAGWCFVIDKPTNRLVVGLGMMPRGEVGLIFLGL
GTSAGLLTPSLEAAILLMVIGTTFLAPVLLRVVLKDKPPSGGNSIPDEIAADPVGLV

Specific function: This is a Na(+)/H(+) antiporter. It can mediate sodium uptake when a transmembrane pH gradient is applied [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 2 (CPA2) transporter (TC 2.A.37) family [H]

Homologues:

Organism=Escherichia coli, GI1786232, Length=430, Percent_Identity=25.1162790697674, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322367, Length=436, Percent_Identity=21.5596330275229, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 47085; Mature: 47085

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLTPLVSALNTHDVEVAETLIGVINFLMIFVAARTLAEILVRLSLPTIVGELLAGVLIG
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASGLHLLLPPSAHAELNQGFVSVISTLASVPKEAVPDIYFETFPSLQAVATLGLYALLFL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHCCHHHHHHHHHHHHHHHH
TGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQVDIIPAIFAGASMTATSIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLQIAPIVKLVLAAT
HHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
VFVFAAIALSRTAAPAFDWLLERLKAPGAVVVASFVILVLSCFVATAIGLEAALGAFAAG
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILSSSKNNHAIQQSVLPLVSLFATIFFVLVGAGMDLSVINPLDPQSRSALIVAGFLFIV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHH
AIVGKIAAGWCFVIDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAGLLTPSLEAAILLMVI
HHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GTTFLAPVLLRVVLKDKPPSGGNSIPDEIAADPVGLV
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure
MVLTPLVSALNTHDVEVAETLIGVINFLMIFVAARTLAEILVRLSLPTIVGELLAGVLIG
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASGLHLLLPPSAHAELNQGFVSVISTLASVPKEAVPDIYFETFPSLQAVATLGLYALLFL
CCCCEEEECCCCHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHCCHHHHHHHHHHHHHHHH
TGLESELEELVAVGAQAFTVAMAGVILPFAFGTFGLMFIFQVDIIPAIFAGASMTATSIG
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ITASVFGELGYLKTREGQIVIGAAVLDDILGIVILAVVVALATGGSLQIAPIVKLVLAAT
HHHHHHHHHCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
VFVFAAIALSRTAAPAFDWLLERLKAPGAVVVASFVILVLSCFVATAIGLEAALGAFAAG
HHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILSSSKNNHAIQQSVLPLVSLFATIFFVLVGAGMDLSVINPLDPQSRSALIVAGFLFIV
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHH
AIVGKIAAGWCFVIDKPTNRLVVGLGMMPRGEVGLIFLGLGTSAGLLTPSLEAAILLMVI
HHHHHHHCCEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHH
GTTFLAPVLLRVVLKDKPPSGGNSIPDEIAADPVGLV
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312090; 1325937 [H]