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The map label for this gene is yugF [H]

Identifier: 124026754

GI number: 124026754

Start: 1692524

End: 1693435

Strand: Reverse

Name: yugF [H]

Synonym: NATL1_20491

Alternate gene names: 124026754

Gene position: 1693435-1692524 (Counterclockwise)

Preceding gene: 124026755

Following gene: 124026753

Centisome position: 90.81

GC content: 39.58

Gene sequence:

>912_bases
ATGGTTTCCTCAGTGGTAGATACAAAACTTTCTGATTGGAAATTCTTAGGTCATCCCGTTCATAGTCTGAGCATTATTCC
TGAAGCTCATAAAACCAAGACTGATGAAGACAAAGGTCCAGCAATTCTTTTAATTCATGGCTTTGGTGCCTCTACAATTC
ATTGGAGGTACAACTTACCAGTACTAGGAAAGCAGTATGAAGTTCACGCTTTAGATCTTCTCGGGTTTGGTAAAAGCTCC
AAACCTAGTGGTCTTGCTTATGGAGGCCCACTTTGGAAAGATCAAATAGTTGCTTACGTAAAAGAAAAAATTGGGAGACC
TACAATTCTTGTAGGTAACTCTCTAGGGGGATATGCTGCGCTTGCTAGTGGTGCGGCGATGGGTCCAGAAGCAGCAGGTG
TGGTTTTATTAAATGCTGCGGGTTATTTCAGTGAAGACAAGAAAACAACTAAAGGAACTTGGGCGACAGCAAGAAAAACT
GTTGCTGGTATCTTTCTCAAAAATGCTCTGTTGCAAAGAATAATTTTTGAAAACCTTAGACAGCCTTCAACAATTAAACG
TACTTTAAATCAAGTTTATATTGATACATCTAACGTTGATGATGAACTAGTAGAAGCGATTCGAAAACCATCTTTGGATA
AGGGTGCTTTTAATGTCTTCAAAAGTGTTTTCGATCCAGCAGGACCTCAGGGAAAACCACTTGATGAGTTATTTTCTCAG
TTGAAGGCACCATTGTTATTGCTTTGGGGTAATAGAGATCCTTGGATGAATGCCCCTGGTAAAAGGGCTACTTATAAAAA
ACACACTCCTACAAATACAAAAGAAGTTGTTTTAGACGCTGGTCATTGTCCTCATGATGAAGTGCCCGATCAGGTTAATT
CCGCTCTTTTAGAATGGATTAATCAGCTCTAA

Upstream 100 bases:

>100_bases
TTTTCAAAAAGGTGAGGCAATTTCGAAAAAAAGAAATAAAATCATTAATCTTTTTCTATCAATTATTTACTAAGATCTAG
AAGTTAGAATTTACTTTTAA

Downstream 100 bases:

>100_bases
TCGATGACCGTCAGTTTCGAAAAAAAGAAAAAGCCTTATCCTCATTGGGAATATTCAAACCCTGAATTCGATTCATCAAT
AAGAATTGTCCCTGAAAGAG

Product: alpha/beta superfamily hydrolase/acyltransferase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MVSSVVDTKLSDWKFLGHPVHSLSIIPEAHKTKTDEDKGPAILLIHGFGASTIHWRYNLPVLGKQYEVHALDLLGFGKSS
KPSGLAYGGPLWKDQIVAYVKEKIGRPTILVGNSLGGYAALASGAAMGPEAAGVVLLNAAGYFSEDKKTTKGTWATARKT
VAGIFLKNALLQRIIFENLRQPSTIKRTLNQVYIDTSNVDDELVEAIRKPSLDKGAFNVFKSVFDPAGPQGKPLDELFSQ
LKAPLLLLWGNRDPWMNAPGKRATYKKHTPTNTKEVVLDAGHCPHDEVPDQVNSALLEWINQL

Sequences:

>Translated_303_residues
MVSSVVDTKLSDWKFLGHPVHSLSIIPEAHKTKTDEDKGPAILLIHGFGASTIHWRYNLPVLGKQYEVHALDLLGFGKSS
KPSGLAYGGPLWKDQIVAYVKEKIGRPTILVGNSLGGYAALASGAAMGPEAAGVVLLNAAGYFSEDKKTTKGTWATARKT
VAGIFLKNALLQRIIFENLRQPSTIKRTLNQVYIDTSNVDDELVEAIRKPSLDKGAFNVFKSVFDPAGPQGKPLDELFSQ
LKAPLLLLWGNRDPWMNAPGKRATYKKHTPTNTKEVVLDAGHCPHDEVPDQVNSALLEWINQL
>Mature_303_residues
MVSSVVDTKLSDWKFLGHPVHSLSIIPEAHKTKTDEDKGPAILLIHGFGASTIHWRYNLPVLGKQYEVHALDLLGFGKSS
KPSGLAYGGPLWKDQIVAYVKEKIGRPTILVGNSLGGYAALASGAAMGPEAAGVVLLNAAGYFSEDKKTTKGTWATARKT
VAGIFLKNALLQRIIFENLRQPSTIKRTLNQVYIDTSNVDDELVEAIRKPSLDKGAFNVFKSVFDPAGPQGKPLDELFSQ
LKAPLLLLWGNRDPWMNAPGKRATYKKHTPTNTKEVVLDAGHCPHDEVPDQVNSALLEWINQL

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33113; Mature: 33113

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSSVVDTKLSDWKFLGHPVHSLSIIPEAHKTKTDEDKGPAILLIHGFGASTIHWRYNLP
CCCCHHHCCCCCCHHHCCCHHHEEECCCHHCCCCCCCCCCEEEEEECCCCCEEEEEECCC
VLGKQYEVHALDLLGFGKSSKPSGLAYGGPLWKDQIVAYVKEKIGRPTILVGNSLGGYAA
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHH
LASGAAMGPEAAGVVLLNAAGYFSEDKKTTKGTWATARKTVAGIFLKNALLQRIIFENLR
HHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPSTIKRTLNQVYIDTSNVDDELVEAIRKPSLDKGAFNVFKSVFDPAGPQGKPLDELFSQ
CCHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
LKAPLLLLWGNRDPWMNAPGKRATYKKHTPTNTKEVVLDAGHCPHDEVPDQVNSALLEWI
HCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
NQL
HCC
>Mature Secondary Structure
MVSSVVDTKLSDWKFLGHPVHSLSIIPEAHKTKTDEDKGPAILLIHGFGASTIHWRYNLP
CCCCHHHCCCCCCHHHCCCHHHEEECCCHHCCCCCCCCCCEEEEEECCCCCEEEEEECCC
VLGKQYEVHALDLLGFGKSSKPSGLAYGGPLWKDQIVAYVKEKIGRPTILVGNSLGGYAA
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHH
LASGAAMGPEAAGVVLLNAAGYFSEDKKTTKGTWATARKTVAGIFLKNALLQRIIFENLR
HHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
QPSTIKRTLNQVYIDTSNVDDELVEAIRKPSLDKGAFNVFKSVFDPAGPQGKPLDELFSQ
CCHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHH
LKAPLLLLWGNRDPWMNAPGKRATYKKHTPTNTKEVVLDAGHCPHDEVPDQVNSALLEWI
HCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
NQL
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]