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The map label for this gene is rpoD4 [H]

Identifier: 124026414

GI number: 124026414

Start: 1394192

End: 1395133

Strand: Reverse

Name: rpoD4 [H]

Synonym: NATL1_17091

Alternate gene names: 124026414

Gene position: 1395133-1394192 (Counterclockwise)

Preceding gene: 124026415

Following gene: 124026412

Centisome position: 74.82

GC content: 40.76

Gene sequence:

>942_bases
ATGGTTTCAACTGCACCCAAGCCTGCTGAATCACAAAAACGACGCAGCAGTGATCCAATTAGTTGGTACCTTTCCTCCAT
AGGACGAGTTCCTCTTTTAACTCCTGCTGAGGAAATTGAACTTGGTAATCAAGTTCAAACTTTGATGAGTCTTACCGAGG
ATGGCCAGATTAAAGAACAGAGCAAAGAATTTACCTCTCATCAAAGAAGATTGATTCGAATTGGAAGAAGGGCAAAGGAA
AGAATGATGAAGGCCAATCTTCGATTGGTTGTCAGTGTTGCAAAGAAATATCAGGGAAAAGGCCTTGAACTTTTAGATTT
AGTTCAGGAAGGTTCTCTTGGGTTAGAAAGAGCGGTAGAAAAATTTGACCCTACTCGTGGATATAAGTTTTCTACTTATG
CATTTTGGTGGATAAGGCAAAGCATGACTAGAGCAATAGCTTGCCAGTCAAGAACAATCCGATTACCTGTTCATTTAAGT
GAGAGATTAGCGACCATTAGAAAAGTCAGCTTAGATTTGGCTCATAAGCTCGGTGCAATGCCCAGCCGTATTGAAATAGC
TGAGGCAATGGAAATTGAGTTAGAAGAACTTGATTCGATTTTGAGACAAGCTCTGACCACAAGCAGTCTTGACGCACCCG
TTAATGGAGATGAAGGTCGTAGCTTTTTAGGTGATCTAATAGCTGACGGTTCTGGAGAAGAACCTCTAGATAAGGTAGAA
CAAAAAATTCATCAAGAACAACTTGGAAGGTGGTTAAGTCATTTAAGCGAACAAGAACAGCACGTTATTAGGCTTAGATT
TGGCTTAGAGGGTAATGAAAGACATACTCTCGCAGAGATAGGCAGATTGTTACAGGTTTCACGCGAGAGGGTAAGGCAAG
TTGAGCTAAAAGCTTTAAGGAAATTGAGAAATCTCACTAGAAAGCTTCCAAGTGGAATTTGA

Upstream 100 bases:

>100_bases
AACTAATCGTCAACAGAATCTGAAGCCTTCATGAAACCTTGGCATCTTTTGCAATTTGTGTAGGAAAGTCTTATCACTTT
AAGTACGGGGCCAAGAAACA

Downstream 100 bases:

>100_bases
TTTTTTATCTAATTTTAGTTTTCTGCCCAAATATATTTTGTTAACTCACTAGGGTCAGGCTCTGGGGCGTTTAAAGCAAA
TTCAACAGCATCGTTAACTT

Product: type II alternative sigma-70 family RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MVSTAPKPAESQKRRSSDPISWYLSSIGRVPLLTPAEEIELGNQVQTLMSLTEDGQIKEQSKEFTSHQRRLIRIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLATIRKVSLDLAHKLGAMPSRIEIAEAMEIELEELDSILRQALTTSSLDAPVNGDEGRSFLGDLIADGSGEEPLDKVE
QKIHQEQLGRWLSHLSEQEQHVIRLRFGLEGNERHTLAEIGRLLQVSRERVRQVELKALRKLRNLTRKLPSGI

Sequences:

>Translated_313_residues
MVSTAPKPAESQKRRSSDPISWYLSSIGRVPLLTPAEEIELGNQVQTLMSLTEDGQIKEQSKEFTSHQRRLIRIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLATIRKVSLDLAHKLGAMPSRIEIAEAMEIELEELDSILRQALTTSSLDAPVNGDEGRSFLGDLIADGSGEEPLDKVE
QKIHQEQLGRWLSHLSEQEQHVIRLRFGLEGNERHTLAEIGRLLQVSRERVRQVELKALRKLRNLTRKLPSGI
>Mature_313_residues
MVSTAPKPAESQKRRSSDPISWYLSSIGRVPLLTPAEEIELGNQVQTLMSLTEDGQIKEQSKEFTSHQRRLIRIGRRAKE
RMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVEKFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLS
ERLATIRKVSLDLAHKLGAMPSRIEIAEAMEIELEELDSILRQALTTSSLDAPVNGDEGRSFLGDLIADGSGEEPLDKVE
QKIHQEQLGRWLSHLSEQEQHVIRLRFGLEGNERHTLAEIGRLLQVSRERVRQVELKALRKLRNLTRKLPSGI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=233, Percent_Identity=46.3519313304721, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI1789098, Length=292, Percent_Identity=39.3835616438356, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789871, Length=247, Percent_Identity=29.5546558704453, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788231, Length=212, Percent_Identity=29.2452830188679, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35618; Mature: 35618

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTAPKPAESQKRRSSDPISWYLSSIGRVPLLTPAEEIELGNQVQTLMSLTEDGQIKEQ
CCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHHH
SKEFTSHQRRLIRIGRRAKERMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
KFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLATIRKVSLDLAHKLGAM
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
PSRIEIAEAMEIELEELDSILRQALTTSSLDAPVNGDEGRSFLGDLIADGSGEEPLDKVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
QKIHQEQLGRWLSHLSEQEQHVIRLRFGLEGNERHTLAEIGRLLQVSRERVRQVELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRNLTRKLPSGI
HHHHHHHHCCCCC
>Mature Secondary Structure
MVSTAPKPAESQKRRSSDPISWYLSSIGRVPLLTPAEEIELGNQVQTLMSLTEDGQIKEQ
CCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCHHHH
SKEFTSHQRRLIRIGRRAKERMMKANLRLVVSVAKKYQGKGLELLDLVQEGSLGLERAVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHH
KFDPTRGYKFSTYAFWWIRQSMTRAIACQSRTIRLPVHLSERLATIRKVSLDLAHKLGAM
HCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCC
PSRIEIAEAMEIELEELDSILRQALTTSSLDAPVNGDEGRSFLGDLIADGSGEEPLDKVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHH
QKIHQEQLGRWLSHLSEQEQHVIRLRFGLEGNERHTLAEIGRLLQVSRERVRQVELKALR
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KLRNLTRKLPSGI
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA