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The map label for this gene is folD

Identifier: 124026222

GI number: 124026222

Start: 1229880

End: 1230791

Strand: Reverse

Name: folD

Synonym: NATL1_15161

Alternate gene names: 124026222

Gene position: 1230791-1229880 (Counterclockwise)

Preceding gene: 124026224

Following gene: 124026221

Centisome position: 66.0

GC content: 39.91

Gene sequence:

>912_bases
ATGCCAAAAATTTTAGATGGAAAAAAACTTGCTCAGGAGATTGAACTCATACTTCAACAGGCTATCGAATCTGGTCTTGA
AGTTGCGAAACGTCGTCCTGGTCTTGCTGTTCTTAGGGTTGGGGATGATCCTGCAAGTGGAGTTTATGTCAATTACAAGG
AAAAAGCATGCGATCGAATAGGTGTTAGAAGTTTTGCAAAGCATTTAAAAGAAGATATTTCATTGGAGGAACTTACAGAA
ACCATTAAAAGCTTGAATGAAGAAAAGAACGTTGATGGGATTTTATTGCAACTACCATTACCTTCGCACCTGGATGAGGC
CGCCTTGCTAAGGAGTATTGATCCTGATAAGGATGCTGATGGTCTACATCCTTTGAACTTAGGAAGATTAATAAAGGGAG
AAAAAGGCCCAAGATCATGCACCCCTGCTGGTGTTATGTCTCTGCTTGAGCGAAATCAAATTAAAATCGAAGGGAAAAGA
GCTGTTGTAGTTGGACGCAGTATTCTTGTTGGTAAGCCAATGGCTTTAATGCTTGAAGCTGCAAATGCCACTGTTACGAT
CGCTCACTCAAGAACTAAAGATTTACCTTCTTTGACTAAGGAGGCAGATTTACTCGTAGTAGCAGCTGGCAAGCCCTATT
TGATTGGGAAAAATCATGTAAGTGAGAATTGCGTTGTTATTGATGTAGGCATTCATAGACTTCCGCCTGATGAAGAAAAT
ATAAAAGAGCTCAAAAAGACTAAACTTTGTGGAGATGTAAAGATATTTGAAATTGAAGATAAAGTAGCTGCTTATTCGCC
TGTCCCAGGCGGTGTAGGGCCTATGACAGTAACAATGCTTCTGGCAAATACGGTTGATAGGTGGCAGAAACATTGTGGAT
TACCTTTAACTATTAGTCATTTACTTCCATGA

Upstream 100 bases:

>100_bases
CAAAATTTTTGTGGGCTGGGTAATTTAGCCAAAGAGTTTCTCACCTACTCTGACGCTAGGTTTAACGCGTTGATTAATTG
AAATCGTATTCATTTTACTT

Downstream 100 bases:

>100_bases
ATCACAATGTCCTGAGAGAATTGGAAAGAATCGATTTTTTGCATGCAGGAAGCAATCGCTTCTTTTGACTTCGCTGAGTA
TCTAGAGAAAGCTAGAACTC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MPKILDGKKLAQEIELILQQAIESGLEVAKRRPGLAVLRVGDDPASGVYVNYKEKACDRIGVRSFAKHLKEDISLEELTE
TIKSLNEEKNVDGILLQLPLPSHLDEAALLRSIDPDKDADGLHPLNLGRLIKGEKGPRSCTPAGVMSLLERNQIKIEGKR
AVVVGRSILVGKPMALMLEAANATVTIAHSRTKDLPSLTKEADLLVVAAGKPYLIGKNHVSENCVVIDVGIHRLPPDEEN
IKELKKTKLCGDVKIFEIEDKVAAYSPVPGGVGPMTVTMLLANTVDRWQKHCGLPLTISHLLP

Sequences:

>Translated_303_residues
MPKILDGKKLAQEIELILQQAIESGLEVAKRRPGLAVLRVGDDPASGVYVNYKEKACDRIGVRSFAKHLKEDISLEELTE
TIKSLNEEKNVDGILLQLPLPSHLDEAALLRSIDPDKDADGLHPLNLGRLIKGEKGPRSCTPAGVMSLLERNQIKIEGKR
AVVVGRSILVGKPMALMLEAANATVTIAHSRTKDLPSLTKEADLLVVAAGKPYLIGKNHVSENCVVIDVGIHRLPPDEEN
IKELKKTKLCGDVKIFEIEDKVAAYSPVPGGVGPMTVTMLLANTVDRWQKHCGLPLTISHLLP
>Mature_302_residues
PKILDGKKLAQEIELILQQAIESGLEVAKRRPGLAVLRVGDDPASGVYVNYKEKACDRIGVRSFAKHLKEDISLEELTET
IKSLNEEKNVDGILLQLPLPSHLDEAALLRSIDPDKDADGLHPLNLGRLIKGEKGPRSCTPAGVMSLLERNQIKIEGKRA
VVVGRSILVGKPMALMLEAANATVTIAHSRTKDLPSLTKEADLLVVAAGKPYLIGKNHVSENCVVIDVGIHRLPPDEENI
KELKKTKLCGDVKIFEIEDKVAAYSPVPGGVGPMTVTMLLANTVDRWQKHCGLPLTISHLLP

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=41.0958904109589, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI94721354, Length=292, Percent_Identity=40.7534246575342, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=37.7551020408163, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI36796743, Length=183, Percent_Identity=26.775956284153, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=45.7746478873239, Blast_Score=236, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17568735, Length=263, Percent_Identity=36.5019011406844, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=40.7017543859649, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=38.9830508474576, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6322933, Length=287, Percent_Identity=24.7386759581882, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=42.3841059602649, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI17136818, Length=302, Percent_Identity=42.3841059602649, Blast_Score=204, Evalue=9e-53,
Organism=Drosophila melanogaster, GI62472483, Length=287, Percent_Identity=39.7212543554007, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45551871, Length=287, Percent_Identity=39.7212543554007, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24645718, Length=287, Percent_Identity=39.7212543554007, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI17137370, Length=287, Percent_Identity=39.7212543554007, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_PROM1 (A2C3L3)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001015338.1
- STRING:   A2C3L3
- GeneID:   4779085
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_15161
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- ProtClustDB:   PRK14186
- BioCyc:   PMAR167555:NATL1_15161-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32956; Mature: 32825

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKILDGKKLAQEIELILQQAIESGLEVAKRRPGLAVLRVGDDPASGVYVNYKEKACDRI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEHHHHHHHH
GVRSFAKHLKEDISLEELTETIKSLNEEKNVDGILLQLPLPSHLDEAALLRSIDPDKDAD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCC
GLHPLNLGRLIKGEKGPRSCTPAGVMSLLERNQIKIEGKRAVVVGRSILVGKPMALMLEA
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEEECCCHHCCCCEEEEEE
ANATVTIAHSRTKDLPSLTKEADLLVVAAGKPYLIGKNHVSENCVVIDVGIHRLPPDEEN
CCCEEEEEECCCCCCCCHHCCCCEEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCHHH
IKELKKTKLCGDVKIFEIEDKVAAYSPVPGGVGPMTVTMLLANTVDRWQKHCGLPLTISH
HHHHHHHHHCCCEEEEEECCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHH
LLP
CCC
>Mature Secondary Structure 
PKILDGKKLAQEIELILQQAIESGLEVAKRRPGLAVLRVGDDPASGVYVNYKEKACDRI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEHHHHHHHH
GVRSFAKHLKEDISLEELTETIKSLNEEKNVDGILLQLPLPSHLDEAALLRSIDPDKDAD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCCCCCCC
GLHPLNLGRLIKGEKGPRSCTPAGVMSLLERNQIKIEGKRAVVVGRSILVGKPMALMLEA
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEEECCCHHCCCCEEEEEE
ANATVTIAHSRTKDLPSLTKEADLLVVAAGKPYLIGKNHVSENCVVIDVGIHRLPPDEEN
CCCEEEEEECCCCCCCCHHCCCCEEEEECCCCEEEECCCCCCCEEEEEECCCCCCCCHHH
IKELKKTKLCGDVKIFEIEDKVAAYSPVPGGVGPMTVTMLLANTVDRWQKHCGLPLTISH
HHHHHHHHHCCCEEEEEECCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEHHH
LLP
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA