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The map label for this gene is iscS [H]

Identifier: 124024958

GI number: 124024958

Start: 225846

End: 226994

Strand: Direct

Name: iscS [H]

Synonym: NATL1_02451

Alternate gene names: 124024958

Gene position: 225846-226994 (Clockwise)

Preceding gene: 124024956

Following gene: 124024960

Centisome position: 12.11

GC content: 38.12

Gene sequence:

>1149_bases
ATGGATAATAATCACCTAATTTTTGATTTTCAGTCTTCAACACCATGTTGTACGAAGGTTGTTGAGGAGATGGCTCCTTA
TTGGAATGAGTTGTGGGGTAATCCCTCTAATACTAATAATCGATCCGGTGTTTTTGCTTCAGCTGCAGTTGAAGTATCTC
GTGAAAAAATAGCTTCATATTTGAATATCAATCCGAAAAGATTAATTTTTACGAGTGGGGCAACAGAAGCGAATAATTTG
GGTTTAGTTGGACATGCAAGAGCTAAAGCACAACTGATTGGAAAACCTGGACACATAATTACCGTTTCAACTGAACATCA
TGCAGTCCTTGATCCGCTTAGGCAGCTTCAAAGAGAAGGGTTTCGCTTGACAGAATTGCAACCGAATAAAGAGGGTTTAA
TCAATATTGAACAACTTTCTGAAGCTTTTGAAAAAGATACTTTTCTGGTTAGCGTCATGGCTGCAAATAATGAGATAGGG
GTTTTGCAACCTATTGGTGACATTGGGTCTTTTTGTAAGAGAAAGGGAATCGCTTTTCATTCTGATGCCGCTCAAGCCTT
TGGATACTTAGATTTAGACCCCGATAAATTTCGCATTGATTTGATGAGTTTGAGTGCTCACAAAATCTATGGACCTAAAG
GTATTGGGGCTTTGGTGATTAGGGAAGGATTCCCTCTTGAACCCTCTCAATATGGAGGAGGTCAGGAGCTTGGATTAAGA
TCTGGCACGCTCCCTGTCCCGTTAATTGTTGGCTTTGCAAAGGCAGTCGAAATAACAAAAAATGATCAGGATGAGAGGAA
CAAGAGACTTTTGTTTTTTAGAAACTTATTGTTGAGTGGATTAAAAAAGAATATTTCTGGATTGATAGTTAATGGATCTA
TTGATCAAAGATTGCCTCATAATCTCAATATCACTTTCCCTGGCGTGAAGGGAAGTCAATTGCATGGTCAATTAAGAAGG
TTTATTTTTTGTACTAGTGGTTCAGCTTGTAGTAATGGTGAAGCTTCTCATGTTCTGCAGGAGATAGGGCTCAGCAAAAA
AGATGCTGAGGCGTCAATAAGGATGAGTATTGGGAGAAATACTACGGAGAAAGATATACATAAGGCTATTAATATTATTA
CGAATATAGTGATTAATCTTAGACAATAA

Upstream 100 bases:

>100_bases
TGTTGATGGATTGGGGTCACTCATAAAATTGGACTTCTAGAGCTTTTGGAGGATATTAACCTTTATTCCTCTCTTATTAT
TTAGATTAACGAATGAATCA

Downstream 100 bases:

>100_bases
TTTAAGACTAAGTTAAGAATTTCTTTGCAGAAATTCTTAACTTTGCACTTTTACTTCTTGGATTTAATTCTATCTCTTCT
GGGCTTGCTCTGATAGGCTT

Product: NifS-like aminotransferase class-V

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MDNNHLIFDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASYLNINPKRLIFTSGATEANNL
GLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQREGFRLTELQPNKEGLINIEQLSEAFEKDTFLVSVMAANNEIG
VLQPIGDIGSFCKRKGIAFHSDAAQAFGYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLR
SGTLPVPLIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPHNLNITFPGVKGSQLHGQLRR
FIFCTSGSACSNGEASHVLQEIGLSKKDAEASIRMSIGRNTTEKDIHKAINIITNIVINLRQ

Sequences:

>Translated_382_residues
MDNNHLIFDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASYLNINPKRLIFTSGATEANNL
GLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQREGFRLTELQPNKEGLINIEQLSEAFEKDTFLVSVMAANNEIG
VLQPIGDIGSFCKRKGIAFHSDAAQAFGYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLR
SGTLPVPLIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPHNLNITFPGVKGSQLHGQLRR
FIFCTSGSACSNGEASHVLQEIGLSKKDAEASIRMSIGRNTTEKDIHKAINIITNIVINLRQ
>Mature_382_residues
MDNNHLIFDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASYLNINPKRLIFTSGATEANNL
GLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQREGFRLTELQPNKEGLINIEQLSEAFEKDTFLVSVMAANNEIG
VLQPIGDIGSFCKRKGIAFHSDAAQAFGYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLR
SGTLPVPLIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPHNLNITFPGVKGSQLHGQLRR
FIFCTSGSACSNGEASHVLQEIGLSKKDAEASIRMSIGRNTTEKDIHKAINIITNIVINLRQ

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=41.2073490813648, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=30.1204819277108, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI48994898, Length=382, Percent_Identity=42.1465968586387, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789175, Length=377, Percent_Identity=26.2599469496021, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1787970, Length=199, Percent_Identity=28.643216080402, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25143064, Length=380, Percent_Identity=41.0526315789474, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=31.7901234567901, Blast_Score=146, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=381, Percent_Identity=41.994750656168, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI20129463, Length=381, Percent_Identity=42.5196850393701, Blast_Score=291, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41920; Mature: 41920

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNNHLIFDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASY
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
LNINPKRLIFTSGATEANNLGLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQREG
HCCCCCEEEEECCCCCCCCCCEEECCCHHHHEECCCCCEEEEECCCHHHHHHHHHHHHCC
FRLTELQPNKEGLINIEQLSEAFEKDTFLVSVMAANNEIGVLQPIGDIGSFCKRKGIAFH
CEEEECCCCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCEEECCCHHHHHHHHHCCCEEE
SDAAQAFGYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLR
CCHHHHCCCCCCCCCCEEEEEEECCCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
SGTLPVPLIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPH
CCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHCCCCC
NLNITFPGVKGSQLHGQLRRFIFCTSGSACSNGEASHVLQEIGLSKKDAEASIRMSIGRN
CCEEEECCCCCHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCC
TTEKDIHKAINIITNIVINLRQ
CCHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MDNNHLIFDFQSSTPCCTKVVEEMAPYWNELWGNPSNTNNRSGVFASAAVEVSREKIASY
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHH
LNINPKRLIFTSGATEANNLGLVGHARAKAQLIGKPGHIITVSTEHHAVLDPLRQLQREG
HCCCCCEEEEECCCCCCCCCCEEECCCHHHHEECCCCCEEEEECCCHHHHHHHHHHHHCC
FRLTELQPNKEGLINIEQLSEAFEKDTFLVSVMAANNEIGVLQPIGDIGSFCKRKGIAFH
CEEEECCCCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCEEECCCHHHHHHHHHCCCEEE
SDAAQAFGYLDLDPDKFRIDLMSLSAHKIYGPKGIGALVIREGFPLEPSQYGGGQELGLR
CCHHHHCCCCCCCCCCEEEEEEECCCCEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
SGTLPVPLIVGFAKAVEITKNDQDERNKRLLFFRNLLLSGLKKNISGLIVNGSIDQRLPH
CCCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHCCCCC
NLNITFPGVKGSQLHGQLRRFIFCTSGSACSNGEASHVLQEIGLSKKDAEASIRMSIGRN
CCEEEECCCCCHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCC
TTEKDIHKAINIITNIVINLRQ
CCHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA