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The map label for this gene is trmD [H]

Identifier: 124023646

GI number: 124023646

Start: 1710654

End: 1711886

Strand: Reverse

Name: trmD [H]

Synonym: P9303_19461

Alternate gene names: 124023646

Gene position: 1711886-1710654 (Counterclockwise)

Preceding gene: 124023647

Following gene: 124023645

Centisome position: 63.81

GC content: 55.39

Gene sequence:

>1233_bases
ATGGCGCCTTTTCGCCTCGATGTAGTGACTTTGGTGCCACAGGCGTTTACTCCTCTTGGTGAACTCGGGGTGATTGGTCG
TGCGTTTGCCTCTGGTATCGCTGCCTTGCACACCCATAATCCCCGCGATTACACCACGGATCGCTACCACAAGGTTGATG
ATCAGCCTTATGGCGGTGGTGCTGGCATGGTGCTTAAGCCAGAACCAGTCTTTGCTGCTTTTGAGGCGATTCCTGTTCAG
TCGCGACGGCGGGTGCTGCTTCTGACCCCTCAGGGAAAACCGCTATGCCAGAAGGATTTGCATCGTTGGGTAGTGGACCA
CGATCAGCTTGTGCTGATCTGCGGGCATTACGAAGGTTTTGATGAGCGGATCAGAGGTTTGGCAGATGAGGAGGTGTCTG
TAGGGGATTTTGTGCTCACCGGTGGTGAGCTACCAGCAATGACGGTGATCAATGGGGTGGTGAGGTTGTTACCGGGAACT
GTTGGAACGGCAGAGTCGCTGCAGGATGAGAGTCATAGCGATGTCCTCCTCGAGCATCCTCACTACACAAGGCCAGCGAA
TTTTCGCGGCATGGTTGTTCCAGATGTGTTGCGCAGTGGTGATCATGGTGCGATTGCTCTGTGGCGACAGCAACAGCGGC
AGCTGCGTACGCAGCTGCGTCGTCCGGATCTCTATGTTCGCTGGTCTGATCAGCAGCAATCATCAACACTGACAATGGAG
TCCCATGGTTCTGCCTCCATGCAGTTTCGTATCGGCAACGGCTATGACATTCATCGCTTAGTGCCCGGTCGCCCCTTGAT
TCTTGGTGGCGTGACACTTGATCATCCCGATGGGTTGGGGCTCGATGGTCACAGCGATGCTGATGTTCTTGTTCATGCGC
TGATGGATGCCTTGCTTGGGGCTCTTTCGTTAGGCGATATCGGTAAATATTTTCCGCCGGATGATCCCCGCTGGAAAGGG
GCCGATAGCTTGATGCTGCTTGAGCAGGTTGTGGCTCTGGTACGAGATCGCGGCTGGCAGGTTTTGAATGTGGATGCCGT
GGTGGTGGCTGAGCGTCCCAAGCTCAAGCCTCATATCAATGCGATGCGCAGCAACCTGGCACAACGGTTGGGGGTGGAAT
TGGATGCCGTGGGTGTGAAGGCCACCACCAATGAAGGGCTTGGACCGGAAGGTCGAGAGGAGGGGATGAGTAGTCAGGCA
GTCGCACTGTTGCAGCAGATAGGCCTGACATGA

Upstream 100 bases:

>100_bases
GGCTGAGGTTATTTCTTCAGGGGTGCTCTGATCAGCGGATGAGCTTGCCTAGTGTCTGTGCGCAGATCCGTTGGTTCTCC
CATTTAGCAGCCTGAAGGAG

Downstream 100 bases:

>100_bases
ATTGCAGAAGCTTGTCTAGGTTGATTTCCTTTGTTCTTAGCTTTCTAGTGATGTTGTCCGGTCATCCTGATGTCGCGATT
GCCGCTCGTCATGACCCTGA

Product: bifunctional enzyme: tRNA methyltransferase; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MAPFRLDVVTLVPQAFTPLGELGVIGRAFASGIAALHTHNPRDYTTDRYHKVDDQPYGGGAGMVLKPEPVFAAFEAIPVQ
SRRRVLLLTPQGKPLCQKDLHRWVVDHDQLVLICGHYEGFDERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGT
VGTAESLQDESHSDVLLEHPHYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQLRRPDLYVRWSDQQQSSTLTME
SHGSASMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLGALSLGDIGKYFPPDDPRWKG
ADSLMLLEQVVALVRDRGWQVLNVDAVVVAERPKLKPHINAMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQA
VALLQQIGLT

Sequences:

>Translated_410_residues
MAPFRLDVVTLVPQAFTPLGELGVIGRAFASGIAALHTHNPRDYTTDRYHKVDDQPYGGGAGMVLKPEPVFAAFEAIPVQ
SRRRVLLLTPQGKPLCQKDLHRWVVDHDQLVLICGHYEGFDERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGT
VGTAESLQDESHSDVLLEHPHYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQLRRPDLYVRWSDQQQSSTLTME
SHGSASMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLGALSLGDIGKYFPPDDPRWKG
ADSLMLLEQVVALVRDRGWQVLNVDAVVVAERPKLKPHINAMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQA
VALLQQIGLT
>Mature_409_residues
APFRLDVVTLVPQAFTPLGELGVIGRAFASGIAALHTHNPRDYTTDRYHKVDDQPYGGGAGMVLKPEPVFAAFEAIPVQS
RRRVLLLTPQGKPLCQKDLHRWVVDHDQLVLICGHYEGFDERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGTV
GTAESLQDESHSDVLLEHPHYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQLRRPDLYVRWSDQQQSSTLTMES
HGSASMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLGALSLGDIGKYFPPDDPRWKGA
DSLMLLEQVVALVRDRGWQVLNVDAVVVAERPKLKPHINAMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQAV
ALLQQIGLT

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=246, Percent_Identity=40.2439024390244, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=50.6410256410256, Blast_Score=135, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 44854; Mature: 44723

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPFRLDVVTLVPQAFTPLGELGVIGRAFASGIAALHTHNPRDYTTDRYHKVDDQPYGGG
CCCCEEHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
AGMVLKPEPVFAAFEAIPVQSRRRVLLLTPQGKPLCQKDLHRWVVDHDQLVLICGHYEGF
CCEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
DERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGTVGTAESLQDESHSDVLLEHP
HHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECC
HYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQLRRPDLYVRWSDQQQSSTLTME
CCCCCCCCCCEECHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
SHGSASMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLG
CCCCCEEEEEECCCCEEEEECCCCCEEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ALSLGDIGKYFPPDDPRWKGADSLMLLEQVVALVRDRGWQVLNVDAVVVAERPKLKPHIN
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHH
AMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQAVALLQQIGLT
HHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
APFRLDVVTLVPQAFTPLGELGVIGRAFASGIAALHTHNPRDYTTDRYHKVDDQPYGGG
CCCEEHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
AGMVLKPEPVFAAFEAIPVQSRRRVLLLTPQGKPLCQKDLHRWVVDHDQLVLICGHYEGF
CCEEECCCCHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCH
DERIRGLADEEVSVGDFVLTGGELPAMTVINGVVRLLPGTVGTAESLQDESHSDVLLEHP
HHHHCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHCCCCCCCEEEECC
HYTRPANFRGMVVPDVLRSGDHGAIALWRQQQRQLRTQLRRPDLYVRWSDQQQSSTLTME
CCCCCCCCCCEECHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEE
SHGSASMQFRIGNGYDIHRLVPGRPLILGGVTLDHPDGLGLDGHSDADVLVHALMDALLG
CCCCCEEEEEECCCCEEEEECCCCCEEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
ALSLGDIGKYFPPDDPRWKGADSLMLLEQVVALVRDRGWQVLNVDAVVVAERPKLKPHIN
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHH
AMRSNLAQRLGVELDAVGVKATTNEGLGPEGREEGMSSQAVALLQQIGLT
HHHHHHHHHHCCEEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA