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The map label for this gene is menD [H]

Identifier: 124023580

GI number: 124023580

Start: 1640931

End: 1642757

Strand: Direct

Name: menD [H]

Synonym: P9303_18801

Alternate gene names: 124023580

Gene position: 1640931-1642757 (Clockwise)

Preceding gene: 124023577

Following gene: 124023581

Centisome position: 61.17

GC content: 55.39

Gene sequence:

>1827_bases
TTGGCTAAGGCTCAAGCTCTGTCTCTTGCTCACAACAATCTGCTGGTTTGCCTGAGCTTGCTCGAGGCATTTCATGCCTT
GGGGCTGCGCCATCTCGTGCTTTGTCCCGGCAGCCGCTCGGGGCCCCTTGCACTTGCTGCAGGAGGAATGGCACGTACCA
ATCTCTTGCGGCTTAGCACTGCCATTGATGAGCGCTCTGCTGCTTTCTTGGCTTTGGGTTTTAGCACTGCTACTGGCACT
GCAGCAGCTGTAGTAACCACTTCGGGCACCGCTGTAGCCAACCTTTTGCCTGCAGCGGTGGAGGCTGATCGATCCTGTCA
GCCCCTGTTGCTGCTCACTGCTGACCGTCCCTATCGACTCAAAGATTGCGGTGCCAATCAAACGGTGAATCAGGAGACTT
TCTTGAGTCCTGCCTGTCGATGGATTGGGCAGGGCCCAAGAGAGGGGTTGCATCTTTTCAGTACAGAAACGCTGGAATCA
TTCGCAGAGGAGGCCTGGCAACGAGCTCATCACCCGGCAGGAGCCGTTCATCTCAATCTGCCTTTTGAGGAGCCTCTACA
TCTCAGTGAAGAAGAGCAGAGGGTGATCTGGAAGGGGTGGTCTCCCAAGATTCCACGTTCAGCTCCGATCACGCCAACAA
ATCTGGCAATGGCAGCTGAGGGGACTGAGGGGGTTACCGATCGGGCTCCCTTTGCATTGGATCCCTTCCGACCCGGCGTT
GTGATAGCTGGTGCTTGGCGAGGCCTAAGTAAAGACCTCTTTGCTTTTCAGCAATCCTTGCGGGAGTGGCAGGCCTTGAG
TGGCTGGCCCGTGCTGGCCGATCCACTATCGGCTCTGCCTTCAGACCAACCTGGTCTAATCCGCTCCTGGGAGCTGCTTT
TGGCTGCTGGGCTTTTAGGCTCTCATGAGCAGCTTCAAGTGCTGCGTCTAGGACCAATGTCAGCCAGTCGGAGCCTTGAG
GCATGGTTGAAAAGCTTTGGTGACGGTCAGCTCCTAATTACAGAGGGAGACTCCCGTTGTCTCGATCCCCTTGGTTTGTC
AGTGCAATGGAGTCATGGATTGACGACCTGGTGGCAACACCATCACCATCGCTGGATCGATGCTGATGCCGCCTCTCAAC
AGGCAACCCAGCCTCTACTGAAGAAATGGCAGATCATCGATCGTTTTGCGCAGGACTGGCTAGATCAGCAGTTACCCCTG
CAAGGTGCCATCACAGAACCAGCCCTGGCGCGCTGGTTATCCCGTCTTCTGCCAGCTGAGCTATCAATCATGTTGGCAGC
GAGTAGTCCTGTGAGGGACTGGCTGGCTTATGCGGACAAGAGCTTATTTTCGAGACGATGCTTCAGTTTTCGAGGCGCGT
CTGGTATCGACGGCACCCTTTCTTTAAGCATGGGTTTAGCGATGGCCCTTGGGCCCACTTTGCTTGTCAGCGGCGATCTA
GCTCTCCTTCATGACAGCAATGGCTGGTTGCTTGCTCATCCCCAGCGCCCGCCTTTGGTGGTGGTGTTGATCGACAATGG
TGGCGGAGGCATCTTTGAGCAGCTGCTGGTGAAGAAAGCTCCGAGCGAAGCATTTGAGCAGCTGTTTGCCATGCCTCAGG
CGGTTGACCCTCTTGTCCTTGCAGCGGCTCATAACATTCCTCACCGTCAGGTGGCCTGTCTCGAAGACCTACCAGCAGCC
TTGGAGTGGGGGCTCTTCCAGACTGGACCTGTTTTGATACGTGTATGCACACATCGGGGTCAAGATTCATCGATGAGGCA
ACAGCTTCGCGAGGGATTGGTGATGCATCTGCAGTCGATTTCCCAAAATGGCCACATTGATCTTTGA

Upstream 100 bases:

>100_bases
CGCCAGCTACCGCTTGCTTCTTTTCGAGGAGGAGAAGGTGTCGGGGTCATCGAAACTGATTAAGCCGGCCCTCGCCGGTC
GGTATTGACCGAGAATCGCA

Downstream 100 bases:

>100_bases
TCCCGATGGCTATGGATTCCAATCGGTTTGATATGAGGCCAAGACAGGTTTTGCCTGGCAAGCCCACGATCGAATGGGAA
CCCTGGGGTGAGTATCAAGA

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 608; Mature: 607

Protein sequence:

>608_residues
MAKAQALSLAHNNLLVCLSLLEAFHALGLRHLVLCPGSRSGPLALAAGGMARTNLLRLSTAIDERSAAFLALGFSTATGT
AAAVVTTSGTAVANLLPAAVEADRSCQPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGPREGLHLFSTETLES
FAEEAWQRAHHPAGAVHLNLPFEEPLHLSEEEQRVIWKGWSPKIPRSAPITPTNLAMAAEGTEGVTDRAPFALDPFRPGV
VIAGAWRGLSKDLFAFQQSLREWQALSGWPVLADPLSALPSDQPGLIRSWELLLAAGLLGSHEQLQVLRLGPMSASRSLE
AWLKSFGDGQLLITEGDSRCLDPLGLSVQWSHGLTTWWQHHHHRWIDADAASQQATQPLLKKWQIIDRFAQDWLDQQLPL
QGAITEPALARWLSRLLPAELSIMLAASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDL
ALLHDSNGWLLAHPQRPPLVVVLIDNGGGGIFEQLLVKKAPSEAFEQLFAMPQAVDPLVLAAAHNIPHRQVACLEDLPAA
LEWGLFQTGPVLIRVCTHRGQDSSMRQQLREGLVMHLQSISQNGHIDL

Sequences:

>Translated_608_residues
MAKAQALSLAHNNLLVCLSLLEAFHALGLRHLVLCPGSRSGPLALAAGGMARTNLLRLSTAIDERSAAFLALGFSTATGT
AAAVVTTSGTAVANLLPAAVEADRSCQPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGPREGLHLFSTETLES
FAEEAWQRAHHPAGAVHLNLPFEEPLHLSEEEQRVIWKGWSPKIPRSAPITPTNLAMAAEGTEGVTDRAPFALDPFRPGV
VIAGAWRGLSKDLFAFQQSLREWQALSGWPVLADPLSALPSDQPGLIRSWELLLAAGLLGSHEQLQVLRLGPMSASRSLE
AWLKSFGDGQLLITEGDSRCLDPLGLSVQWSHGLTTWWQHHHHRWIDADAASQQATQPLLKKWQIIDRFAQDWLDQQLPL
QGAITEPALARWLSRLLPAELSIMLAASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDL
ALLHDSNGWLLAHPQRPPLVVVLIDNGGGGIFEQLLVKKAPSEAFEQLFAMPQAVDPLVLAAAHNIPHRQVACLEDLPAA
LEWGLFQTGPVLIRVCTHRGQDSSMRQQLREGLVMHLQSISQNGHIDL
>Mature_607_residues
AKAQALSLAHNNLLVCLSLLEAFHALGLRHLVLCPGSRSGPLALAAGGMARTNLLRLSTAIDERSAAFLALGFSTATGTA
AAVVTTSGTAVANLLPAAVEADRSCQPLLLLTADRPYRLKDCGANQTVNQETFLSPACRWIGQGPREGLHLFSTETLESF
AEEAWQRAHHPAGAVHLNLPFEEPLHLSEEEQRVIWKGWSPKIPRSAPITPTNLAMAAEGTEGVTDRAPFALDPFRPGVV
IAGAWRGLSKDLFAFQQSLREWQALSGWPVLADPLSALPSDQPGLIRSWELLLAAGLLGSHEQLQVLRLGPMSASRSLEA
WLKSFGDGQLLITEGDSRCLDPLGLSVQWSHGLTTWWQHHHHRWIDADAASQQATQPLLKKWQIIDRFAQDWLDQQLPLQ
GAITEPALARWLSRLLPAELSIMLAASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDLA
LLHDSNGWLLAHPQRPPLVVVLIDNGGGGIFEQLLVKKAPSEAFEQLFAMPQAVDPLVLAAAHNIPHRQVACLEDLPAAL
EWGLFQTGPVLIRVCTHRGQDSSMRQQLREGLVMHLQSISQNGHIDL

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=530, Percent_Identity=32.8301886792453, Blast_Score=170, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 66038; Mature: 65907

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAQALSLAHNNLLVCLSLLEAFHALGLRHLVLCPGSRSGPLALAAGGMARTNLLRLST
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHH
AIDERSAAFLALGFSTATGTAAAVVTTSGTAVANLLPAAVEADRSCQPLLLLTADRPYRL
HHCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEE
KDCGANQTVNQETFLSPACRWIGQGPREGLHLFSTETLESFAEEAWQRAHHPAGAVHLNL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PFEEPLHLSEEEQRVIWKGWSPKIPRSAPITPTNLAMAAEGTEGVTDRAPFALDPFRPGV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCCE
VIAGAWRGLSKDLFAFQQSLREWQALSGWPVLADPLSALPSDQPGLIRSWELLLAAGLLG
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHCHHHHCCCCCCCHHHHHHHHHHHHHCC
SHEQLQVLRLGPMSASRSLEAWLKSFGDGQLLITEGDSRCLDPLGLSVQWSHGLTTWWQH
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHH
HHHRWIDADAASQQATQPLLKKWQIIDRFAQDWLDQQLPLQGAITEPALARWLSRLLPAE
HCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHH
LSIMLAASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDL
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCE
ALLHDSNGWLLAHPQRPPLVVVLIDNGGGGIFEQLLVKKAPSEAFEQLFAMPQAVDPLVL
EEEECCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHH
AAAHNIPHRQVACLEDLPAALEWGLFQTGPVLIRVCTHRGQDSSMRQQLREGLVMHLQSI
HHHCCCCCHHHHHHHHCHHHHHCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
SQNGHIDL
CCCCCCCC
>Mature Secondary Structure 
AKAQALSLAHNNLLVCLSLLEAFHALGLRHLVLCPGSRSGPLALAAGGMARTNLLRLST
CCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHH
AIDERSAAFLALGFSTATGTAAAVVTTSGTAVANLLPAAVEADRSCQPLLLLTADRPYRL
HHCCCCCEEEEEECCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEE
KDCGANQTVNQETFLSPACRWIGQGPREGLHLFSTETLESFAEEAWQRAHHPAGAVHLNL
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PFEEPLHLSEEEQRVIWKGWSPKIPRSAPITPTNLAMAAEGTEGVTDRAPFALDPFRPGV
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCCE
VIAGAWRGLSKDLFAFQQSLREWQALSGWPVLADPLSALPSDQPGLIRSWELLLAAGLLG
EEEECCCCCHHHHHHHHHHHHHHHHCCCCCCHHCHHHHCCCCCCCHHHHHHHHHHHHHCC
SHEQLQVLRLGPMSASRSLEAWLKSFGDGQLLITEGDSRCLDPLGLSVQWSHGLTTWWQH
CCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHH
HHHRWIDADAASQQATQPLLKKWQIIDRFAQDWLDQQLPLQGAITEPALARWLSRLLPAE
HCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHH
LSIMLAASSPVRDWLAYADKSLFSRRCFSFRGASGIDGTLSLSMGLAMALGPTLLVSGDL
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCEEEEECCE
ALLHDSNGWLLAHPQRPPLVVVLIDNGGGGIFEQLLVKKAPSEAFEQLFAMPQAVDPLVL
EEEECCCCEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHH
AAAHNIPHRQVACLEDLPAALEWGLFQTGPVLIRVCTHRGQDSSMRQQLREGLVMHLQSI
HHHCCCCCHHHHHHHHCHHHHHCCCCCCCHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
SQNGHIDL
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA