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The map label for this gene is trmA [C]

Identifier: 124023185

GI number: 124023185

Start: 1277157

End: 1278554

Strand: Reverse

Name: trmA [C]

Synonym: P9303_14811

Alternate gene names: 124023185

Gene position: 1278554-1277157 (Counterclockwise)

Preceding gene: 124023186

Following gene: 124023183

Centisome position: 47.66

GC content: 41.7

Gene sequence:

>1398_bases
ATGACTAGCTCTACAAACTCAAGTTTGACATCAACACCAACCCCAGGTGATAAGGTTGAGATCACATGCGTAGATTTAGA
TCGTCATGGTAATGGACTGGCGCGATGGAACAATTGGGTTGTGATAGTCAATGACCTTCTGCCTGGTGAACGAGCACAAA
TAGAGTTTGTGAGACGCCATCGAAACCAGTTTTTAGCCCGAAAGGTTCATTCAATTGAGACAGTTTCAAGTCGCAGACAG
CCCCCATGCAAAATCGCCAAAGAATGTGGAGGTTGCTCAATACAGCACCTTAATGATCATGGACAGAGCTTATTAAAGCA
AAGTCATCTACGGGAAATACTTCTACGTTTAGGTCAATTGGATAATAAAATAGAACCAATAATGACTGATTATACTAGAG
CTCTCGGATATCGAAATAGAGCCTTAATTCCCCTTTATCGCAATAGAGAAGGACAGTTACTTATGGGTTATTACCGACGC
GGCAGTCACCAAATTATTGATCTCAATCGATGTCCGGTACTGGACCGAGGAATCGATCAATATCTGGCTTGGATCAAAGA
TGATCTTCAACAGCAGAATTGGCCGGCAGATTCAGACCTATCTTCTGGCGAAGGAGTCCGTCATCTAGGCATTCGTATTG
GCCAAAAAACAAGACAAGTGCTGATCACACTGGTAAGTAGTACCACTGAACTCTCTGGCCTACATCAACAAGCCAACGAC
TGGTATAGGCGTTGGCCGGAAGTAAAAGGAATAACTTTAAACATCCAACGACAGAGAAACAATCTTGTTCTTGGTCAAGA
AACTATATTGCTTGCTGGTGATCTTGATATTGAGGAGCATTTTTGTAATCTCTCCTTATTGCTTTCTACCACAACATTTT
TTCAAGTGAACACTATTCAGGCAGAACGGATAGTCTTAAAATTACTTAAATGGCTATCCACTACCTTGCCTGACATCAAT
ATAATTGACGCATATTGTGGAATTGGCACAATTTCTCTCCCCATCGCAGCAGCTGGATATCACGTAATAGGTCTTGAACT
TCATGCAGAAGCTATTGCTCAAGCAAATAAAAATGCCTTGCGGAATAATCTGGGTAATGCTTCGTTTTTGTGTGGTGATG
TTTCCTTGTTACTGGCCAAAATACTTCAACATCATGATGTGCTAGTGCTGGATCCACCGCGTAAAGGTTTAGATCAAATG
GTGATTAAAACAATCCTTTCAGTTCGACCAGAGAGAGTGGCATATCTTAGTTGTGACCCTGCCACATTGGCTCGTGACCT
CAAGCAATTGATAGTCCCAAATGGACCCTATCAGATTGATCAATTACAACCAATTGATTTCTTCCCACAAACAATGCATA
TCGAATGTCTGGCACTGTTAATTCGTATTAGCTCCTGA

Upstream 100 bases:

>100_bases
TTATGTATCCCAAACCCAATACCACATATCTGGATATTTACAATTTATTTATAGACAGTGTTGACTTTATTGTTATGTCT
AATCAATAGGAAGACTTGTT

Downstream 100 bases:

>100_bases
GCTCTGCAGAAAACTGCTTTACAAGTGCTTGACGTACCTTCTCGTGAATCGGGTTGACCCTGTCTTCACTAAGAGTGCTG
TCTTTACTTCGATAACGCAA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MTSSTNSSLTSTPTPGDKVEITCVDLDRHGNGLARWNNWVVIVNDLLPGERAQIEFVRRHRNQFLARKVHSIETVSSRRQ
PPCKIAKECGGCSIQHLNDHGQSLLKQSHLREILLRLGQLDNKIEPIMTDYTRALGYRNRALIPLYRNREGQLLMGYYRR
GSHQIIDLNRCPVLDRGIDQYLAWIKDDLQQQNWPADSDLSSGEGVRHLGIRIGQKTRQVLITLVSSTTELSGLHQQAND
WYRRWPEVKGITLNIQRQRNNLVLGQETILLAGDLDIEEHFCNLSLLLSTTTFFQVNTIQAERIVLKLLKWLSTTLPDIN
IIDAYCGIGTISLPIAAAGYHVIGLELHAEAIAQANKNALRNNLGNASFLCGDVSLLLAKILQHHDVLVLDPPRKGLDQM
VIKTILSVRPERVAYLSCDPATLARDLKQLIVPNGPYQIDQLQPIDFFPQTMHIECLALLIRISS

Sequences:

>Translated_465_residues
MTSSTNSSLTSTPTPGDKVEITCVDLDRHGNGLARWNNWVVIVNDLLPGERAQIEFVRRHRNQFLARKVHSIETVSSRRQ
PPCKIAKECGGCSIQHLNDHGQSLLKQSHLREILLRLGQLDNKIEPIMTDYTRALGYRNRALIPLYRNREGQLLMGYYRR
GSHQIIDLNRCPVLDRGIDQYLAWIKDDLQQQNWPADSDLSSGEGVRHLGIRIGQKTRQVLITLVSSTTELSGLHQQAND
WYRRWPEVKGITLNIQRQRNNLVLGQETILLAGDLDIEEHFCNLSLLLSTTTFFQVNTIQAERIVLKLLKWLSTTLPDIN
IIDAYCGIGTISLPIAAAGYHVIGLELHAEAIAQANKNALRNNLGNASFLCGDVSLLLAKILQHHDVLVLDPPRKGLDQM
VIKTILSVRPERVAYLSCDPATLARDLKQLIVPNGPYQIDQLQPIDFFPQTMHIECLALLIRISS
>Mature_464_residues
TSSTNSSLTSTPTPGDKVEITCVDLDRHGNGLARWNNWVVIVNDLLPGERAQIEFVRRHRNQFLARKVHSIETVSSRRQP
PCKIAKECGGCSIQHLNDHGQSLLKQSHLREILLRLGQLDNKIEPIMTDYTRALGYRNRALIPLYRNREGQLLMGYYRRG
SHQIIDLNRCPVLDRGIDQYLAWIKDDLQQQNWPADSDLSSGEGVRHLGIRIGQKTRQVLITLVSSTTELSGLHQQANDW
YRRWPEVKGITLNIQRQRNNLVLGQETILLAGDLDIEEHFCNLSLLLSTTTFFQVNTIQAERIVLKLLKWLSTTLPDINI
IDAYCGIGTISLPIAAAGYHVIGLELHAEAIAQANKNALRNNLGNASFLCGDVSLLLAKILQHHDVLVLDPPRKGLDQMV
IKTILSVRPERVAYLSCDPATLARDLKQLIVPNGPYQIDQLQPIDFFPQTMHIECLALLIRISS

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=204, Percent_Identity=35.2941176470588, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI51173878, Length=204, Percent_Identity=35.2941176470588, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI269784678, Length=388, Percent_Identity=25.7731958762887, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI269784680, Length=357, Percent_Identity=26.6106442577031, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI269784676, Length=357, Percent_Identity=26.6106442577031, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI20149688, Length=357, Percent_Identity=26.6106442577031, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1789148, Length=450, Percent_Identity=28.8888888888889, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1787083, Length=329, Percent_Identity=26.1398176291793, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1790403, Length=172, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24666579, Length=443, Percent_Identity=24.1534988713318, Blast_Score=109, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52442; Mature: 52311

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSTNSSLTSTPTPGDKVEITCVDLDRHGNGLARWNNWVVIVNDLLPGERAQIEFVRRH
CCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEECCEEEEEECCCCCCHHHHHHHHHH
RNQFLARKVHSIETVSSRRQPPCKIAKECGGCSIQHLNDHGQSLLKQSHLREILLRLGQL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
DNKIEPIMTDYTRALGYRNRALIPLYRNREGQLLMGYYRRGSHQIIDLNRCPVLDRGIDQ
CCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCCCHHCCHHH
YLAWIKDDLQQQNWPADSDLSSGEGVRHLGIRIGQKTRQVLITLVSSTTELSGLHQQAND
HHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHH
WYRRWPEVKGITLNIQRQRNNLVLGQETILLAGDLDIEEHFCNLSLLLSTTTFFQVNTIQ
HHHHCCCCCCEEEEEEECCCCEEECCCEEEEEECCCHHHHHCCEEEEEEEEEEEEEECHH
AERIVLKLLKWLSTTLPDINIIDAYCGIGTISLPIAAAGYHVIGLELHAEAIAQANKNAL
HHHHHHHHHHHHHHCCCCCEEEEHHCCCCCEECCHHHCCEEEEEEEHHHHHHHHHHHHHH
RNNLGNASFLCGDVSLLLAKILQHHDVLVLDPPRKGLDQMVIKTILSVRPERVAYLSCDP
HHCCCCCEEEHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCH
ATLARDLKQLIVPNGPYQIDQLQPIDFFPQTMHIECLALLIRISS
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSSTNSSLTSTPTPGDKVEITCVDLDRHGNGLARWNNWVVIVNDLLPGERAQIEFVRRH
CCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEECCEEEEEECCCCCCHHHHHHHHHH
RNQFLARKVHSIETVSSRRQPPCKIAKECGGCSIQHLNDHGQSLLKQSHLREILLRLGQL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCC
DNKIEPIMTDYTRALGYRNRALIPLYRNREGQLLMGYYRRGSHQIIDLNRCPVLDRGIDQ
CCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEEECCCCCCHHCCHHH
YLAWIKDDLQQQNWPADSDLSSGEGVRHLGIRIGQKTRQVLITLVSSTTELSGLHQQAND
HHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHCHHHHHHHHHHHHH
WYRRWPEVKGITLNIQRQRNNLVLGQETILLAGDLDIEEHFCNLSLLLSTTTFFQVNTIQ
HHHHCCCCCCEEEEEEECCCCEEECCCEEEEEECCCHHHHHCCEEEEEEEEEEEEEECHH
AERIVLKLLKWLSTTLPDINIIDAYCGIGTISLPIAAAGYHVIGLELHAEAIAQANKNAL
HHHHHHHHHHHHHHCCCCCEEEEHHCCCCCEECCHHHCCEEEEEEEHHHHHHHHHHHHHH
RNNLGNASFLCGDVSLLLAKILQHHDVLVLDPPRKGLDQMVIKTILSVRPERVAYLSCDP
HHCCCCCEEEHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECCH
ATLARDLKQLIVPNGPYQIDQLQPIDFFPQTMHIECLALLIRISS
HHHHHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]