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The map label for this gene is pheT [H]

Identifier: 124023184

GI number: 124023184

Start: 1274720

End: 1277167

Strand: Direct

Name: pheT [H]

Synonym: P9303_14801

Alternate gene names: 124023184

Gene position: 1274720-1277167 (Clockwise)

Preceding gene: 124023180

Following gene: 124023192

Centisome position: 47.52

GC content: 49.35

Gene sequence:

>2448_bases
ATGCGGGTTTCTCTCTCCTGGCTGCAGGATCTAGTACAGCTGAATGAGACCGTCGAGAACCTCGCCGAAGGCCTGTCGAT
GGCTGGATTTGAGGTTGAGGCTATTGATGATTTAGCCAGCCGTGCCGATGGCGTGGTCGTTGGGTTGGTGAAAGACCATC
AACCCCACCCCAATGCAGACAAACTCAGCGTCTGTCAGGTGGATGTTGGGTCGGGTGAATGTCTTCAGATCGTCTGTGGG
GCTCCAAATGTTCGGCTTGGTATACACGTACCTGTTGCCATGGTTGGTGCAACCCTCCCAGCAGTTGGAATCAAGATCAA
AGCCAGTGAGCTTCGGGGGGTGCCAAGTCAAGGAATGATCTGCTCTCTATCGGAATTAGGACTGGAATCAAATGGTGATG
GAATAGCCATCCTTGAAGAGATCGCTGAAAACGTTCCAGAGCTCGGCCAGCCTGTAGCTCCTCTGCTCGGACTCGATGAC
ACAGTGCTTGAGTTGGCAATCACAGCCAATCGCCCCGATGGAATGTCGATGGTTGGTATCGCAAGGGAAGTCGCTGCTTT
AACAGGTGCAATCCTGCAGCTTCCGCAACTTGATATGGCCCCAATCCATAAATCTTTCAAGCCTGATTCCACCAGTTCTG
TGTCCATGCTGAAGGGTGGTCTTTATGGCTTAACCGCCTTAGAGAATGTAGATGGTGGATTAACTTCTCCTGCATGGTTA
AAACAACGCTTGGAGCGTTCAGGATTGAAATCAGTGAATGGTGTTGTTGATATTACAAATCTGGTGATGTTGGAGCAAGG
CCAGCCATTGCATGCATTTGATATTGATGCTCTCGAATTGATCACAGGTCAAACAGTTTCCGCAGAATCTTTTGGCCTTC
GACAAGCAAGAAACAATGAAGTTTTTAATGGCCTAGATGGACATCAACTTGCACTGAACGAAAATTGCCAGATCGTCACC
TGCCATGACATTCCTATAGCGCTTGCGGGTGTTATCGGTAGTGCTGAAAGCGGCGTGTCTGCAAAGACAAGGCGAGTATG
GCTGGAATCTGCAATGTTTACCCCAACGGCCGTGAGAACGACCTGCCGAGCTGTTGGCTTGAGGACCGATGCAAGCAGTC
GCTTTGAGAAAGGGTTGCCTGTTGAAATGACTTTGGCATCAGCGCGTCGAGCTGTGACACTAATGGAGGAACATCTAGGC
ATTAAATCAAATGGATGTTGGGTATATGGTGAGCCCCTAAAAACTGCTGAGCCGGTAAAACTTCGCCGCGAAGCTATCCA
TCGCTTACTCGGACCAATAGCTGATGAGAATGACAATAGGTATCTAGAAGACGACATTGTCGAGACTTCGTTGCTTGCTC
TCGGATGCGAGTTGTCTCCTTATAACGAAGGGTGGTTGGTCATAGTTCCACCATCACGCCGTAGAGATCTCTCGCGAGAG
GTTGATTTGATCGAAGAGGTGTCTCGCCTTGTTGGCTTTGATCGTTTTGAGGCCAACCTGCCAGATCCGCTTGAACCTGG
TGGGCTAACGACTGCTCAAACAGCTGAACGCCGATTGAGGCAGATGTTGTGCGGAGCAGGATTGCAGGAAGTCACCACCT
TGTCGCTAGTGGGTGCTGATAGTGATGAACCACAACGAATTGCAATTAGCAACCCTTTATTGGCTGAGACCAGCCACCTG
CGTACCAATCTTTGGGAGGAGCATCTACGTATTTGCCAACGTAACCTCCAGTCATCGCAGCCTGGTTGTTGGTTATATGA
AATAGGCAATGTCTATACAGTCACTGACGAGCTCATCAACCAACGCGCTGTTCTTGGTGGCGTGATCTGTGCAGAGCGAA
GGTTTGAACGATGGAGTACAAGCGGCAAGATCAAGTCAATGACATATCACCAGGCGCGCGGTCAGCTTTCCCAAGTCTTT
CAAGGGTTGAAGTTGGACATTAACGACCGTCCCCTCAAGGACAATCCTTCACTTCATCCTGGACGGTCTGCAGAGTTATT
TGTCGAAGGCAAACTGCTCGGGGATTTTGGGCAGCTTCATCCAGCACTATCGGAACGTCTTGATCTACCTGAAGCAACCT
ATCTATTCGCGTTGGACTTTCAGTGCATCATCCAAGCTGCGACACGCTCTAATCGATGGAACCCTATCTTCCGACCATTC
CCGACAGTTCCAGCAATGGAACTTGATTTAGCTGTGATCGTTTCAAGGGAATGTAGTTGTTCAGATTTGATCCAAGCCAT
CAGAAAAGCTGGTAAACCGCTGCTAGAGCATGTAGAGCTGATTGATCGTTTTGAGGGTGGACAGCTTGATCATGACTCTT
GCAGTCAAGCGTTCAGGTTGCGTTATCGAAGTAAAGACAGCACTCTTAGTGAAGACAGGGTCAACCCGATTCACGAGAAG
GTACGTCAAGCACTTGTAAAGCAGTTTTCTGCAGAGCTCAGGAGCTAA

Upstream 100 bases:

>100_bases
GAAGCGTAGTGAAGAACTAAGATCCTACAGGGCAGCCTCACATGACTTGACGCCTTCGTTCGCTTGCTTCGTAAGATCTC
TTGCGGCCTCTTGCTGATCC

Downstream 100 bases:

>100_bases
TACGAATTAACAGTGCCAGACATTCGATATGCATTGTTTGTGGGAAGAAATCAATTGGTTGTAATTGATCAATCTGATAG
GGTCCATTTGGGACTATCAA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 815; Mature: 815

Protein sequence:

>815_residues
MRVSLSWLQDLVQLNETVENLAEGLSMAGFEVEAIDDLASRADGVVVGLVKDHQPHPNADKLSVCQVDVGSGECLQIVCG
APNVRLGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLSELGLESNGDGIAILEEIAENVPELGQPVAPLLGLDD
TVLELAITANRPDGMSMVGIAREVAALTGAILQLPQLDMAPIHKSFKPDSTSSVSMLKGGLYGLTALENVDGGLTSPAWL
KQRLERSGLKSVNGVVDITNLVMLEQGQPLHAFDIDALELITGQTVSAESFGLRQARNNEVFNGLDGHQLALNENCQIVT
CHDIPIALAGVIGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLG
IKSNGCWVYGEPLKTAEPVKLRREAIHRLLGPIADENDNRYLEDDIVETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERRLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHL
RTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERRFERWSTSGKIKSMTYHQARGQLSQVF
QGLKLDINDRPLKDNPSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDFQCIIQAATRSNRWNPIFRPF
PTVPAMELDLAVIVSRECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDHDSCSQAFRLRYRSKDSTLSEDRVNPIHEK
VRQALVKQFSAELRS

Sequences:

>Translated_815_residues
MRVSLSWLQDLVQLNETVENLAEGLSMAGFEVEAIDDLASRADGVVVGLVKDHQPHPNADKLSVCQVDVGSGECLQIVCG
APNVRLGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLSELGLESNGDGIAILEEIAENVPELGQPVAPLLGLDD
TVLELAITANRPDGMSMVGIAREVAALTGAILQLPQLDMAPIHKSFKPDSTSSVSMLKGGLYGLTALENVDGGLTSPAWL
KQRLERSGLKSVNGVVDITNLVMLEQGQPLHAFDIDALELITGQTVSAESFGLRQARNNEVFNGLDGHQLALNENCQIVT
CHDIPIALAGVIGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLG
IKSNGCWVYGEPLKTAEPVKLRREAIHRLLGPIADENDNRYLEDDIVETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERRLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHL
RTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERRFERWSTSGKIKSMTYHQARGQLSQVF
QGLKLDINDRPLKDNPSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDFQCIIQAATRSNRWNPIFRPF
PTVPAMELDLAVIVSRECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDHDSCSQAFRLRYRSKDSTLSEDRVNPIHEK
VRQALVKQFSAELRS
>Mature_815_residues
MRVSLSWLQDLVQLNETVENLAEGLSMAGFEVEAIDDLASRADGVVVGLVKDHQPHPNADKLSVCQVDVGSGECLQIVCG
APNVRLGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMICSLSELGLESNGDGIAILEEIAENVPELGQPVAPLLGLDD
TVLELAITANRPDGMSMVGIAREVAALTGAILQLPQLDMAPIHKSFKPDSTSSVSMLKGGLYGLTALENVDGGLTSPAWL
KQRLERSGLKSVNGVVDITNLVMLEQGQPLHAFDIDALELITGQTVSAESFGLRQARNNEVFNGLDGHQLALNENCQIVT
CHDIPIALAGVIGSAESGVSAKTRRVWLESAMFTPTAVRTTCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLG
IKSNGCWVYGEPLKTAEPVKLRREAIHRLLGPIADENDNRYLEDDIVETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERRLRQMLCGAGLQEVTTLSLVGADSDEPQRIAISNPLLAETSHL
RTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELINQRAVLGGVICAERRFERWSTSGKIKSMTYHQARGQLSQVF
QGLKLDINDRPLKDNPSLHPGRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDFQCIIQAATRSNRWNPIFRPF
PTVPAMELDLAVIVSRECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDHDSCSQAFRLRYRSKDSTLSEDRVNPIHEK
VRQALVKQFSAELRS

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=831, Percent_Identity=32.6113116726835, Blast_Score=374, Evalue=1e-104,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 89100; Mature: 89100

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSLSWLQDLVQLNETVENLAEGLSMAGFEVEAIDDLASRADGVVVGLVKDHQPHPNAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
KLSVCQVDVGSGECLQIVCGAPNVRLGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMI
CEEEEEEECCCCCEEEEEECCCCEEEEEECHHHHHHCCCCCCCEEEEHHHHCCCCCCCCE
CSLSELGLESNGDGIAILEEIAENVPELGQPVAPLLGLDDTVLELAITANRPDGMSMVGI
EEHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHEEEEEECCCCCCCHHHHH
AREVAALTGAILQLPQLDMAPIHKSFKPDSTSSVSMLKGGLYGLTALENVDGGLTSPAWL
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCHHHH
KQRLERSGLKSVNGVVDITNLVMLEQGQPLHAFDIDALELITGQTVSAESFGLRQARNNE
HHHHHHCCCHHHCCCEEHHHEEEECCCCCCEEECCCHHHHHCCCCCCCHHHCHHHHCCCC
VFNGLDGHQLALNENCQIVTCHDIPIALAGVIGSAESGVSAKTRRVWLESAMFTPTAVRT
CCCCCCCCEEEECCCCCEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHH
TCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLGIKSNGCWVYGEPLKTAEPVK
HHHHHCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH
LRREAIHRLLGPIADENDNRYLEDDIVETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERRLRQMLCGAGLQEVTTLSLVGAD
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
SDEPQRIAISNPLLAETSHLRTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELIN
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHH
QRAVLGGVICAERRFERWSTSGKIKSMTYHQARGQLSQVFQGLKLDINDRPLKDNPSLHP
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
GRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDFQCIIQAATRSNRWNPIFRPF
CCCCEEEEEEEEECCHHHHCHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCC
PTVPAMELDLAVIVSRECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDHDSCSQAFRL
CCCCCHHCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RYRSKDSTLSEDRVNPIHEKVRQALVKQFSAELRS
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVSLSWLQDLVQLNETVENLAEGLSMAGFEVEAIDDLASRADGVVVGLVKDHQPHPNAD
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCC
KLSVCQVDVGSGECLQIVCGAPNVRLGIHVPVAMVGATLPAVGIKIKASELRGVPSQGMI
CEEEEEEECCCCCEEEEEECCCCEEEEEECHHHHHHCCCCCCCEEEEHHHHCCCCCCCCE
CSLSELGLESNGDGIAILEEIAENVPELGQPVAPLLGLDDTVLELAITANRPDGMSMVGI
EEHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCHHHEEEEEECCCCCCCHHHHH
AREVAALTGAILQLPQLDMAPIHKSFKPDSTSSVSMLKGGLYGLTALENVDGGLTSPAWL
HHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHCCCCCCCCHHHH
KQRLERSGLKSVNGVVDITNLVMLEQGQPLHAFDIDALELITGQTVSAESFGLRQARNNE
HHHHHHCCCHHHCCCEEHHHEEEECCCCCCEEECCCHHHHHCCCCCCCHHHCHHHHCCCC
VFNGLDGHQLALNENCQIVTCHDIPIALAGVIGSAESGVSAKTRRVWLESAMFTPTAVRT
CCCCCCCCEEEECCCCCEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHH
TCRAVGLRTDASSRFEKGLPVEMTLASARRAVTLMEEHLGIKSNGCWVYGEPLKTAEPVK
HHHHHCCCCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHH
LRREAIHRLLGPIADENDNRYLEDDIVETSLLALGCELSPYNEGWLVIVPPSRRRDLSRE
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
VDLIEEVSRLVGFDRFEANLPDPLEPGGLTTAQTAERRLRQMLCGAGLQEVTTLSLVGAD
HHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHEEEEEECC
SDEPQRIAISNPLLAETSHLRTNLWEEHLRICQRNLQSSQPGCWLYEIGNVYTVTDELIN
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHHHH
QRAVLGGVICAERRFERWSTSGKIKSMTYHQARGQLSQVFQGLKLDINDRPLKDNPSLHP
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCC
GRSAELFVEGKLLGDFGQLHPALSERLDLPEATYLFALDFQCIIQAATRSNRWNPIFRPF
CCCCEEEEEEEEECCHHHHCHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCCCCCC
PTVPAMELDLAVIVSRECSCSDLIQAIRKAGKPLLEHVELIDRFEGGQLDHDSCSQAFRL
CCCCCHHCCEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RYRSKDSTLSEDRVNPIHEKVRQALVKQFSAELRS
HHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]