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The map label for this gene is pntA [H]

Identifier: 124022569

GI number: 124022569

Start: 777595

End: 778788

Strand: Direct

Name: pntA [H]

Synonym: P9303_08601

Alternate gene names: 124022569

Gene position: 777595-778788 (Clockwise)

Preceding gene: 124022567

Following gene: 124022570

Centisome position: 28.99

GC content: 55.11

Gene sequence:

>1194_bases
GTGCCGGGAGGTCGACCCCGTAATCTCGCCTGGTTCAACCAGTCCTGTCTTTTGCCAAGACTCTTAATTCCGGTGGAATC
GGCGATGGGAGAAACCCGTGTTGCGGCCTCGCCGGACACGGTGAAGAAGTTTCTTTCTCTGGGCAGCAAGGTTGCCATTG
AGCGTGGCGCCGGCCTTGCCGCAGGTTTTGTTGATGGTGATTTCAACACCATTGGTGCGGAGCTGGTTGCTCCGGGAGAT
TCACAGGCCTGGAGCGAGGCCGATGTCGTCCTATGCGTTCAATCTCCTTCTGCTAGTGCTTTGGCAAGGATGCGATCTGG
GGCCCTTGTTGTTGGTTTGTTGGCGCCTTATGCCAACAGTTCTTTAGCAGCGTCGCTGAAGACAGCAAAGCTCTCAGCGA
TGGCCCTAGAGCTGCTGCCGAGAATTAGCCGGGCACAGTCTGCGGATGCCCTTTCCTCTCAGGCCAATATTGCTGGCTAC
AAGGCGGTGCTGTTGGCTGCTGCTGCCCTTGATCGTTACTTTCCGATGTTGATGACGGCAGCTGGCACGGTGCAGCCAGC
GCGGGTCGTGGTCTTAGGTGCTGGTGTGGCTGGATTGCAGGCAGTTGCTACAGCAAAGCGTCTGGGTGCTGTGGTCTATG
TCAGTGATGTCCGCTCTGCCGTGAAGGAGCAGGTGGAGTCACTTGGGGCACGCTTTATCGAACCTCCTGAAATTGATCAG
AGGCCAGATGAGTCTGGTGGCTATGCCAAGCAGGCTTCTGAGGCTTTTTTGACGGCTCAGCGTGAGCAGCTCTCCAACCA
GTTAGCGCAAGCCGATGTGGTGATCTGTACTGCTCAGGTGCCTGGTCGAAAAGCTCCACGATTAATTAGTGATCTCATGC
TCGATCGCATGCGCCCAGGTGCTGTGGTGGTCGATCTTGCCGTTGAACAGGGTGGAAATTGTGCGAATACTCGTCCTGGT
GAAACAGTCGATCGCAATGGCGTGAAGTTGATCGGTGCCTCCGATCTCCCTTGCAGTGTTCCCAATCACGCCAGTGCTCT
TTACTCTCGCAATTTGGTCGCTCTGTTGGAGCCCGTTTTGAAGGATGGTCAGCTCAGTTTGAACATTGAGGATGAGTTAA
TTGCCGGATGCCTGATCAGTCACAACGGTGTTATTCGCCATGACGATGTGCTTAACACAGGAGGGTCCAACTGA

Upstream 100 bases:

>100_bases
GACATTCGATTGCAGTTAGGCCCATGGAGGGAGTTGGAGTTAACAATCCTTCTTAGCTATAAAGCCAGCTGAAAAACTCT
TTTCTTCAGGAAAGCTCCCG

Downstream 100 bases:

>100_bases
TGTCGTTCTTGAGTGAGGCCCTTTGGGTGCTGCTGCTTGGCAGCTTGCTTGGCCTTGAACTGATCGGCAAGGTGCCTCCC
ACTTTGCATACGCCTTTGAT

Product: nicotinamide nucleotide transhydrogenase, subunit alpha 1 (A1)

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPGGRPRNLAWFNQSCLLPRLLIPVESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDGDFNTIGAELVAPGD
SQAWSEADVVLCVQSPSASALARMRSGALVVGLLAPYANSSLAASLKTAKLSAMALELLPRISRAQSADALSSQANIAGY
KAVLLAAAALDRYFPMLMTAAGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIEPPEIDQ
RPDESGGYAKQASEAFLTAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANTRPG
ETVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGCLISHNGVIRHDDVLNTGGSN

Sequences:

>Translated_397_residues
MPGGRPRNLAWFNQSCLLPRLLIPVESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDGDFNTIGAELVAPGD
SQAWSEADVVLCVQSPSASALARMRSGALVVGLLAPYANSSLAASLKTAKLSAMALELLPRISRAQSADALSSQANIAGY
KAVLLAAAALDRYFPMLMTAAGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIEPPEIDQ
RPDESGGYAKQASEAFLTAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANTRPG
ETVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGCLISHNGVIRHDDVLNTGGSN
>Mature_396_residues
PGGRPRNLAWFNQSCLLPRLLIPVESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLAAGFVDGDFNTIGAELVAPGDS
QAWSEADVVLCVQSPSASALARMRSGALVVGLLAPYANSSLAASLKTAKLSAMALELLPRISRAQSADALSSQANIAGYK
AVLLAAAALDRYFPMLMTAAGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIEPPEIDQR
PDESGGYAKQASEAFLTAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPGAVVVDLAVEQGGNCANTRPGE
TVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVLKDGQLSLNIEDELIAGCLISHNGVIRHDDVLNTGGSN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=381, Percent_Identity=37.7952755905512, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI122939153, Length=381, Percent_Identity=37.7952755905512, Blast_Score=240, Evalue=2e-63,
Organism=Escherichia coli, GI1787887, Length=369, Percent_Identity=44.7154471544715, Blast_Score=291, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17550456, Length=367, Percent_Identity=41.6893732970027, Blast_Score=284, Evalue=5e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 41339; Mature: 41208

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGGRPRNLAWFNQSCLLPRLLIPVESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLA
CCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHCCCCEEEECCCCEE
AGFVDGDFNTIGAELVAPGDSQAWSEADVVLCVQSPSASALARMRSGALVVGLLAPYANS
EEEECCCCHHCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEECCCCCCC
SLAASLKTAKLSAMALELLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPMLMTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIEPPEIDQ
CCCCCCCEEEEEECCHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPDESGGYAKQASEAFLTAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
AVVVDLAVEQGGNCANTRPGETVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVL
CEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHH
KDGQLSLNIEDELIAGCLISHNGVIRHDDVLNTGGSN
CCCEEEEEECHHHHHHHHHCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
PGGRPRNLAWFNQSCLLPRLLIPVESAMGETRVAASPDTVKKFLSLGSKVAIERGAGLA
CCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEECCCHHHHHHHHHCCCCEEEECCCCEE
AGFVDGDFNTIGAELVAPGDSQAWSEADVVLCVQSPSASALARMRSGALVVGLLAPYANS
EEEECCCCHHCCCEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHCCCEEEEECCCCCCC
SLAASLKTAKLSAMALELLPRISRAQSADALSSQANIAGYKAVLLAAAALDRYFPMLMTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
AGTVQPARVVVLGAGVAGLQAVATAKRLGAVVYVSDVRSAVKEQVESLGARFIEPPEIDQ
CCCCCCCEEEEEECCHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCC
RPDESGGYAKQASEAFLTAQREQLSNQLAQADVVICTAQVPGRKAPRLISDLMLDRMRPG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
AVVVDLAVEQGGNCANTRPGETVDRNGVKLIGASDLPCSVPNHASALYSRNLVALLEPVL
CEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHH
KDGQLSLNIEDELIAGCLISHNGVIRHDDVLNTGGSN
CCCEEEEEECHHHHHHHHHCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]