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The map label for this gene is glgX [H]

Identifier: 124022279

GI number: 124022279

Start: 548162

End: 550276

Strand: Reverse

Name: glgX [H]

Synonym: P9303_05691

Alternate gene names: 124022279

Gene position: 550276-548162 (Counterclockwise)

Preceding gene: 124022287

Following gene: 124022278

Centisome position: 20.51

GC content: 55.22

Gene sequence:

>2115_bases
GTGCCCAATGCTTTCTGCCTCGCCGCAGACACAGCTGTAAGCAAGATGCATCTCGGCACTCCTTGGCCCCTGGGCAGCAC
GATCACAACACGAGGCGTCAATTTCTCTGTGGCCGCCCCAACTGCAAAGCGCGTGGAGCTGCTGCTGTTTGCCGATGCAA
ATGCCACGCAGCCAGAGCACATCCTCGAGCTCCAGCCTTGCCATCGATCCGGCGACTACTGGCATGTTGAGGTGGAAGGA
TTGCGAACTGGCTGCTGTTATGGCTATCGGATCTTCGGCCCCCACAACACTGGCAGCGATAGCTTTCACCCCTCCAAGGT
GTTGCTTGATCCCTGCACAAGAGCCATCACTGGCTGGGATATCTATCAAAGAGAACAGGCCAAGGGATCGTCGCCCAATA
TCCAAGCCTGCCTAAAGGGAGTGGTGTGCGAACGAGAACATTTTGACTTCGCATCCCATCCACGCCCCAGGACCCCATGG
AACCGCAGCGTGATCTACGAACTGCATGTAGGGGGGTTCAGCAGCGGCCCGAAGTCAGAGGTAACACCTCAGCGCCAAGG
CACCTTCCTGGGACTCATTGACAAACTTCCCTATCTGCGACAACTGGGTATCACGGCCATAGAGCTGCTACCTATTTTTG
CTTTCGATCCCTCTGATGCGCCAACGGGGCGGGACAACTACTGGGGCTACAGCCCTCTGAACTGGTTCACTCCCCATCCC
AAATATGTTCATGGTGACGATCCATTGCAGGCTCGCCAACAAGTCCGCGAGCTGGTTGCGGCCTGTCATGACGAGGGTAT
TGAAGTCATCCTTGACGTGGTCTACAACCACACCACTGAAGGCAGCCTTGATGGCCCCACACTCAGCTGGCGAGGCTTCA
GCGATGCTCTCTATTACCACCAAAACGACAGTGGCGAATACCTGGATGTAAGTGGTTGCGGCAACAGCATCGCCGCCAAT
CGTCCAATCGTGAGGCAGCTGATTCTCGAATCGATGCGTTGCTGGGCCATCGAACTAGGAGTCGATGGCTTCCGCTTCGA
TCTAGGGATCGCCCTGAGCCGAGGCGAAGGCCTAAAGCCGCTCGAACACCCTCCCCTATTTGAAGAGATTGAAGCTGATC
CAGAGCTCAGCGATTTAAAACTGTTTAGCGAACCATGGGATTGCGGTGGCCTCTATCGACTCAGCGATTTCCCAGCACAA
CGAATCGGAACATGGAATGGCCATTACCGAGATGATCTACGTGCCTTCTGGAAAGGAGACGAAGACAGCGCCTGGAGGAT
GGGACAACGCCTAAGCGGAAGTGCTGATCTCTACAAAGGTGAACCAGCTCCACTGGGACGTTCCCTGAACTTCATCACTG
CACACGATGGCTTCACCCTCAATGACCTGGTGAGTTTCAACCGCAAACACAACCTCGCCAATGGAGAGAGCAACCGCGAC
GGTGAAAATCACAACAACAGCTGGAACAACGGCATTGAAGGCCCCTGCAGTGACCATGCCGTGCAAGCCCTCCGCCATCG
CCAGATGCGCAATCTGATCGCCACCCTGTTGCTTTCAAGAGGAGTGCCAATGCTGTTGATGGGAGATGAAGTGGGCCGCA
GCCAGGGGGGTAACAACAACAGCTGGTGTCAGGACAACCCGCTCGGCTGGATGCTCTGGAACCCTGAACAATGCGATATG
GATTTGCACCTCTTCGTCAGCAGGCTGCTGATGATTCGCCAGCAACTTTCGGAACTCTTCTGTCCCATCAATCCCCCCCC
TGAAGAGATGCCCGTAAGTCTTCAGCAGAGTCCTGACGATCTATGGCTGCAATGGCATGGTGTTGAGATCGACAAGCCCG
ATTGGGGCAGCTGGTCCCATACCATCTGCTACAGCCTGAATCGAGGCTCCAGCGGTGCGGTGATGTGGATGGGCTTCAAC
GCCTACAGCAAAGCCATGCATTTTGACCTGCCTAAACCGACTTCTGCGTGGCATCTCCTCCTGGACACAGCATGTCCCAG
CCCAGATGATCTACCCGCAAGACCTAATGCATGGACTTCAGTGGACATTGATCTGCAGAACCGCAGCATGTTGATCTTGA
TGGCAGAGGAATACGCCTCAAAACTCAAGCTCTAA

Upstream 100 bases:

>100_bases
AAAACCCAGAGGGGCCCGCTCAGCTGCTCTGGAGGATTGCCCGACTCCAATGCTTGGGACAACGTCATGACATTGTGAAA
CGGGCTGAACCTCTTCGCTT

Downstream 100 bases:

>100_bases
AAGGCAAAATCAAAAAAGCGGGCGGCGGGAATCGAACCCGCGTCATCAGCTTGGAAGGCTGAGGTCTTACCACTACACAA
CGCCCGCACTAGTACAACAG

Product: isoamylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MPNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEHILELQPCHRSGDYWHVEVEG
LRTGCCYGYRIFGPHNTGSDSFHPSKVLLDPCTRAITGWDIYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPW
NRSVIYELHVGGFSSGPKSEVTPQRQGTFLGLIDKLPYLRQLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSLDGPTLSWRGFSDALYYHQNDSGEYLDVSGCGNSIAAN
RPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKPLEHPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAQ
RIGTWNGHYRDDLRAFWKGDEDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGESNRD
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNNSWCQDNPLGWMLWNPEQCDM
DLHLFVSRLLMIRQQLSELFCPINPPPEEMPVSLQQSPDDLWLQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFN
AYSKAMHFDLPKPTSAWHLLLDTACPSPDDLPARPNAWTSVDIDLQNRSMLILMAEEYASKLKL

Sequences:

>Translated_704_residues
MPNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEHILELQPCHRSGDYWHVEVEG
LRTGCCYGYRIFGPHNTGSDSFHPSKVLLDPCTRAITGWDIYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPW
NRSVIYELHVGGFSSGPKSEVTPQRQGTFLGLIDKLPYLRQLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSLDGPTLSWRGFSDALYYHQNDSGEYLDVSGCGNSIAAN
RPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKPLEHPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAQ
RIGTWNGHYRDDLRAFWKGDEDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGESNRD
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNNSWCQDNPLGWMLWNPEQCDM
DLHLFVSRLLMIRQQLSELFCPINPPPEEMPVSLQQSPDDLWLQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFN
AYSKAMHFDLPKPTSAWHLLLDTACPSPDDLPARPNAWTSVDIDLQNRSMLILMAEEYASKLKL
>Mature_703_residues
PNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEHILELQPCHRSGDYWHVEVEGL
RTGCCYGYRIFGPHNTGSDSFHPSKVLLDPCTRAITGWDIYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPWN
RSVIYELHVGGFSSGPKSEVTPQRQGTFLGLIDKLPYLRQLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHPK
YVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSLDGPTLSWRGFSDALYYHQNDSGEYLDVSGCGNSIAANR
PIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKPLEHPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAQR
IGTWNGHYRDDLRAFWKGDEDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGESNRDG
ENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNNSWCQDNPLGWMLWNPEQCDMD
LHLFVSRLLMIRQQLSELFCPINPPPEEMPVSLQQSPDDLWLQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFNA
YSKAMHFDLPKPTSAWHLLLDTACPSPDDLPARPNAWTSVDIDLQNRSMLILMAEEYASKLKL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=577, Percent_Identity=40.3812824956672, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 79005; Mature: 78873

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEH
CCCCEEEEECHHHHHEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCHH
ILELQPCHRSGDYWHVEVEGLRTGCCYGYRIFGPHNTGSDSFHPSKVLLDPCTRAITGWD
EEEECCCCCCCCEEEEEECCCEECEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCH
IYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPWNRSVIYELHVGGFSSGPKSE
HHHHHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
VTPQRQGTFLGLIDKLPYLRQLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
CCCCCCCCCHHHHHHCHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSLDGPTLSWRGFSDALYYH
CEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEE
QNDSGEYLDVSGCGNSIAANRPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKP
ECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEECCCCCCC
LEHPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAQRIGTWNGHYRDDLRAFWKGD
CCCCCCHHHHCCCCCHHHHEEECCCCCCCCEEEECCCCHHHCCCCCCCHHHHHHHHHCCC
EDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGESNRD
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCC
SWCQDNPLGWMLWNPEQCDMDLHLFVSRLLMIRQQLSELFCPINPPPEEMPVSLQQSPDD
CCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCC
LWLQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFNAYSKAMHFDLPKPTSAWHLL
EEEEEECEEECCCCCCCCCCEEEEEECCCCCCEEEEEECCHHHHHEECCCCCCCCCEEEE
LDTACPSPDDLPARPNAWTSVDIDLQNRSMLILMAEEYASKLKL
EECCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHCCC
>Mature Secondary Structure 
PNAFCLAADTAVSKMHLGTPWPLGSTITTRGVNFSVAAPTAKRVELLLFADANATQPEH
CCCEEEEECHHHHHEECCCCCCCCCCEEECCCEEEEECCCCCEEEEEEEECCCCCCCHH
ILELQPCHRSGDYWHVEVEGLRTGCCYGYRIFGPHNTGSDSFHPSKVLLDPCTRAITGWD
EEEECCCCCCCCEEEEEECCCEECEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCH
IYQREQAKGSSPNIQACLKGVVCEREHFDFASHPRPRTPWNRSVIYELHVGGFSSGPKSE
HHHHHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCC
VTPQRQGTFLGLIDKLPYLRQLGITAIELLPIFAFDPSDAPTGRDNYWGYSPLNWFTPHP
CCCCCCCCCHHHHHHCHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
KYVHGDDPLQARQQVRELVAACHDEGIEVILDVVYNHTTEGSLDGPTLSWRGFSDALYYH
CEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEEEE
QNDSGEYLDVSGCGNSIAANRPIVRQLILESMRCWAIELGVDGFRFDLGIALSRGEGLKP
ECCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEEEEECCCCCCC
LEHPPLFEEIEADPELSDLKLFSEPWDCGGLYRLSDFPAQRIGTWNGHYRDDLRAFWKGD
CCCCCCHHHHCCCCCHHHHEEECCCCCCCCEEEECCCCHHHCCCCCCCHHHHHHHHHCCC
EDSAWRMGQRLSGSADLYKGEPAPLGRSLNFITAHDGFTLNDLVSFNRKHNLANGESNRD
CCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCC
GENHNNSWNNGIEGPCSDHAVQALRHRQMRNLIATLLLSRGVPMLLMGDEVGRSQGGNNN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCCCC
SWCQDNPLGWMLWNPEQCDMDLHLFVSRLLMIRQQLSELFCPINPPPEEMPVSLQQSPDD
CCCCCCCCEEEEECCHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCC
LWLQWHGVEIDKPDWGSWSHTICYSLNRGSSGAVMWMGFNAYSKAMHFDLPKPTSAWHLL
EEEEEECEEECCCCCCCCCCEEEEEECCCCCCEEEEEECCHHHHHEECCCCCCCCCEEEE
LDTACPSPDDLPARPNAWTSVDIDLQNRSMLILMAEEYASKLKL
EECCCCCCCCCCCCCCCCEEEEEEECCCEEEEEEEHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]