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The map label for this gene is pepB [H]

Identifier: 124022267

GI number: 124022267

Start: 538563

End: 540083

Strand: Reverse

Name: pepB [H]

Synonym: P9303_05571

Alternate gene names: 124022267

Gene position: 540083-538563 (Counterclockwise)

Preceding gene: 124022268

Following gene: 124022256

Centisome position: 20.13

GC content: 57.53

Gene sequence:

>1521_bases
ATGCAGCTAAACGGCACGGCTAACATCGCGCCACCTCATCAACATCCCATGGAGATTTGCCTTTTTCCAGCAACCTTGCA
GAGCTGGACTGGCGATGTGCTCATGGTGGGCATGTTTGAGGGGAAGATGGAGGAACGTTTAAACGAGCTGGAAACACTCT
GCAAAGGCTCTCTGATGCAGAGCCTTGAGAAGCAGATGTTCAAAGGCAAATCCGGAGAAATCGCCACCGTTCAACTGCTC
CAAAACAAGCCAAGCCTGCTGGTGCTCGTAGGGCTCGGAGAACCCCAGAAGATGAGGCTTGACGACCTTCGCAAGGCGGC
TGCCCTGGGAGCGAAAGCAAGCCTCGGATGCAGTGGAACCCTGGGGATAATGCTCCCCTGGGAGCCCTTAGATGCGGCAT
CTGCCGCAAGAGCCGTCGCAGAAGCCGTTCGCCTTTCCCTCTATAAAGACCTCCGCTTTCGCAGTGCTCCTGAGCCCCGC
TCTACGCCAACAAAACTGGAGCTCATTGGTCTGCCCGACTCAGCCGGCAAAGACCTTCAAGCCGTTCACCCCACCTGTGC
GGGCGTGGAGCTAGCAAGGCAGCTGGTTGCGGCTCCTGCCAACAGTCTTACCCCTGCGGCCCTCGCCCAAACAGCGATTC
AACTTGCCCACGAACACGGCCTCGAGTGCACTGTGCTGGAGCGGTCTGACTGCGCCGAACGGGAAATGGGTGCGTACCTA
GCTGTCTCTCAAGGTTCTGATCTGGAGCCAAAATTCATTCACCTCACCTATCGCCCTCAAGGACCAGTTCAACGACGACT
GGCCCTTGTAGGCAAGGGACTGACCTTCGATTCCGGTGGATACAACCTCAAGGTTGGAGCTGCCCAGATCGATCTGATGA
AGTTCGACATGGGCGGCAGCGCAGCTGTACTTGGGGCCGCCCGAGCGATTGCAGAACTGCGACCCAAGGGAGTGGAAGTG
CATGTGATCGTGGCAGCCTGCGAAAACATGGTGAATGGATCCGCTGTCCATCCAGGAGACATCGTGCGGGCCTCAAACGG
CACGACCATCGAGATCAACAACACCGATGCCGAAGGCCGTCTGACCCTTGCCGATGCACTGGTATACACCTGCGGACTCG
AACCAGACGCAATCGTGGATTTAGCAACCCTTACAGGGGCTTGCGTGATCGCACTTGGCGAAGAGATCGCTGGCCTTTGG
ACAGGTCATGATCCCCTGGCTGAGGGACTAACCGCTGCCGCCGAGGCGGCCGGCGAAGGCCTTTGGCGAATGCCATTGCC
AAGCTCCTATCGAGAGGGTCTCAAATCCAACCTCGCTGACCTCAAGAACACAGGACCTCGTCCTGGCGGATCCATTACCG
CGGCCCTTTTCCTCAAAGAGTTTGTTGAAGCCTCAATCCCATGGGCCCACATCGACATCGCCGGAACGGTCTGGTCTGAA
AAGGGCCGTGGCCTCAATCCATCTGGTGCCACCGGCTACGGGGTTCGCACTTTGGTGAATTGGATTTGTAGTCAGTCTTG
A

Upstream 100 bases:

>100_bases
CCAAAGCCCGTCGACAGCTCGAGCAACAACTGATCCAGCGCTGGCTGCCACCGTTCAACAAAGAAACAAGAGCTCGCTGG
AGCACCCCGTTCACAGCTGA

Downstream 100 bases:

>100_bases
GTCAAGAGGCTTTGCATTCAACTCAGCAAAAAATGAGCTGAAGAAGGAATCACAACACGAGTACAACGTTGATCGCTAAA
GGTCGCGCAGGGTTGGCAGT

Product: leucyl aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MQLNGTANIAPPHQHPMEICLFPATLQSWTGDVLMVGMFEGKMEERLNELETLCKGSLMQSLEKQMFKGKSGEIATVQLL
QNKPSLLVLVGLGEPQKMRLDDLRKAAALGAKASLGCSGTLGIMLPWEPLDAASAARAVAEAVRLSLYKDLRFRSAPEPR
STPTKLELIGLPDSAGKDLQAVHPTCAGVELARQLVAAPANSLTPAALAQTAIQLAHEHGLECTVLERSDCAEREMGAYL
AVSQGSDLEPKFIHLTYRPQGPVQRRLALVGKGLTFDSGGYNLKVGAAQIDLMKFDMGGSAAVLGAARAIAELRPKGVEV
HVIVAACENMVNGSAVHPGDIVRASNGTTIEINNTDAEGRLTLADALVYTCGLEPDAIVDLATLTGACVIALGEEIAGLW
TGHDPLAEGLTAAAEAAGEGLWRMPLPSSYREGLKSNLADLKNTGPRPGGSITAALFLKEFVEASIPWAHIDIAGTVWSE
KGRGLNPSGATGYGVRTLVNWICSQS

Sequences:

>Translated_506_residues
MQLNGTANIAPPHQHPMEICLFPATLQSWTGDVLMVGMFEGKMEERLNELETLCKGSLMQSLEKQMFKGKSGEIATVQLL
QNKPSLLVLVGLGEPQKMRLDDLRKAAALGAKASLGCSGTLGIMLPWEPLDAASAARAVAEAVRLSLYKDLRFRSAPEPR
STPTKLELIGLPDSAGKDLQAVHPTCAGVELARQLVAAPANSLTPAALAQTAIQLAHEHGLECTVLERSDCAEREMGAYL
AVSQGSDLEPKFIHLTYRPQGPVQRRLALVGKGLTFDSGGYNLKVGAAQIDLMKFDMGGSAAVLGAARAIAELRPKGVEV
HVIVAACENMVNGSAVHPGDIVRASNGTTIEINNTDAEGRLTLADALVYTCGLEPDAIVDLATLTGACVIALGEEIAGLW
TGHDPLAEGLTAAAEAAGEGLWRMPLPSSYREGLKSNLADLKNTGPRPGGSITAALFLKEFVEASIPWAHIDIAGTVWSE
KGRGLNPSGATGYGVRTLVNWICSQS
>Mature_506_residues
MQLNGTANIAPPHQHPMEICLFPATLQSWTGDVLMVGMFEGKMEERLNELETLCKGSLMQSLEKQMFKGKSGEIATVQLL
QNKPSLLVLVGLGEPQKMRLDDLRKAAALGAKASLGCSGTLGIMLPWEPLDAASAARAVAEAVRLSLYKDLRFRSAPEPR
STPTKLELIGLPDSAGKDLQAVHPTCAGVELARQLVAAPANSLTPAALAQTAIQLAHEHGLECTVLERSDCAEREMGAYL
AVSQGSDLEPKFIHLTYRPQGPVQRRLALVGKGLTFDSGGYNLKVGAAQIDLMKFDMGGSAAVLGAARAIAELRPKGVEV
HVIVAACENMVNGSAVHPGDIVRASNGTTIEINNTDAEGRLTLADALVYTCGLEPDAIVDLATLTGACVIALGEEIAGLW
TGHDPLAEGLTAAAEAAGEGLWRMPLPSSYREGLKSNLADLKNTGPRPGGSITAALFLKEFVEASIPWAHIDIAGTVWSE
KGRGLNPSGATGYGVRTLVNWICSQS

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=488, Percent_Identity=35.0409836065574, Blast_Score=225, Evalue=6e-59,
Organism=Homo sapiens, GI47155554, Length=387, Percent_Identity=31.7829457364341, Blast_Score=139, Evalue=5e-33,
Organism=Escherichia coli, GI1790710, Length=514, Percent_Identity=35.9922178988327, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI87082123, Length=353, Percent_Identity=38.5269121813031, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17556903, Length=307, Percent_Identity=33.8762214983713, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565172, Length=360, Percent_Identity=27.5, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21355725, Length=368, Percent_Identity=33.4239130434783, Blast_Score=173, Evalue=3e-43,
Organism=Drosophila melanogaster, GI24661038, Length=368, Percent_Identity=33.1521739130435, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI20129969, Length=492, Percent_Identity=28.6585365853659, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21355645, Length=483, Percent_Identity=28.3643892339545, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24662223, Length=483, Percent_Identity=28.3643892339545, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24662227, Length=411, Percent_Identity=28.2238442822384, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI161077148, Length=321, Percent_Identity=31.7757009345794, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI20130057, Length=321, Percent_Identity=31.7757009345794, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI20129963, Length=447, Percent_Identity=29.082774049217, Blast_Score=142, Evalue=8e-34,
Organism=Drosophila melanogaster, GI19922386, Length=424, Percent_Identity=28.7735849056604, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI21357381, Length=328, Percent_Identity=30.1829268292683, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI221379063, Length=328, Percent_Identity=30.1829268292683, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI221379062, Length=328, Percent_Identity=30.1829268292683, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646701, Length=324, Percent_Identity=28.7037037037037, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24646703, Length=324, Percent_Identity=28.7037037037037, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI21358201, Length=324, Percent_Identity=28.7037037037037, Blast_Score=99, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042
- InterPro:   IPR008283 [H]

Pfam domain/function: PF00883 Peptidase_M17; PF02789 Peptidase_M17_N [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 53405; Mature: 53405

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLNGTANIAPPHQHPMEICLFPATLQSWTGDVLMVGMFEGKMEERLNELETLCKGSLMQ
CCCCCCCCCCCCCCCCCEEEEECHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
SLEKQMFKGKSGEIATVQLLQNKPSLLVLVGLGEPQKMRLDDLRKAAALGAKASLGCSGT
HHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCC
LGIMLPWEPLDAASAARAVAEAVRLSLYKDLRFRSAPEPRSTPTKLELIGLPDSAGKDLQ
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCH
AVHPTCAGVELARQLVAAPANSLTPAALAQTAIQLAHEHGLECTVLERSDCAEREMGAYL
HCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCEE
AVSQGSDLEPKFIHLTYRPQGPVQRRLALVGKGLTFDSGGYNLKVGAAQIDLMKFDMGGS
EECCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEECCCCCEEEECCEEEEEEEECCCCC
AAVLGAARAIAELRPKGVEVHVIVAACENMVNGSAVHPGDIVRASNGTTIEINNTDAEGR
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCCCCCE
LTLADALVYTCGLEPDAIVDLATLTGACVIALGEEIAGLWTGHDPLAEGLTAAAEAAGEG
EEHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCHHHHHEECCCCHHHHHHHHHHHHHCCC
LWRMPLPSSYREGLKSNLADLKNTGPRPGGSITAALFLKEFVEASIPWAHIDIAGTVWSE
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECC
KGRGLNPSGATGYGVRTLVNWICSQS
CCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MQLNGTANIAPPHQHPMEICLFPATLQSWTGDVLMVGMFEGKMEERLNELETLCKGSLMQ
CCCCCCCCCCCCCCCCCEEEEECHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHH
SLEKQMFKGKSGEIATVQLLQNKPSLLVLVGLGEPQKMRLDDLRKAAALGAKASLGCSGT
HHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCC
LGIMLPWEPLDAASAARAVAEAVRLSLYKDLRFRSAPEPRSTPTKLELIGLPDSAGKDLQ
EEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCH
AVHPTCAGVELARQLVAAPANSLTPAALAQTAIQLAHEHGLECTVLERSDCAEREMGAYL
HCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCCCEE
AVSQGSDLEPKFIHLTYRPQGPVQRRLALVGKGLTFDSGGYNLKVGAAQIDLMKFDMGGS
EECCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEECCCCCEEEECCEEEEEEEECCCCC
AAVLGAARAIAELRPKGVEVHVIVAACENMVNGSAVHPGDIVRASNGTTIEINNTDAEGR
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCCCEEECCCCCEEEEECCCCCCE
LTLADALVYTCGLEPDAIVDLATLTGACVIALGEEIAGLWTGHDPLAEGLTAAAEAAGEG
EEHHHHHHHHCCCCCHHHHHHHHHHHHHHEECCHHHHHEECCCCHHHHHHHHHHHHHCCC
LWRMPLPSSYREGLKSNLADLKNTGPRPGGSITAALFLKEFVEASIPWAHIDIAGTVWSE
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEEECC
KGRGLNPSGATGYGVRTLVNWICSQS
CCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]