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The map label for this gene is purD [H]

Identifier: 124022256

GI number: 124022256

Start: 528423

End: 529739

Strand: Reverse

Name: purD [H]

Synonym: P9303_05461

Alternate gene names: 124022256

Gene position: 529739-528423 (Counterclockwise)

Preceding gene: 124022267

Following gene: 124022255

Centisome position: 19.75

GC content: 58.92

Gene sequence:

>1317_bases
ATGGCCATGTCCAGCACCCGTCCCCACCCCCTCCCCCCTTTAGAGCGGCTGCTTGTTGTTGGCAGTGGTGGGCGTGAAAA
TGCCCTTGCATGGGCACTACGACGCTGTTCCGGCATAGAAGATGTCTGGGTCACACCTGGGAATGGGGGCACAAAAGAGC
ACCAGGGATGCGGGTGCCTGGCGATCGCAGAAACCGACGTCAATGGCCTGATTGATGCCTGCCAATCGCTTCAGATCGAT
CTAGTGGTAATAGGGCCGGAAGCTCCACTGGCCGCTGGCGTGGCTGACGGACTGCGCAAGGTCGGGCTAGCTGTATTTGG
GCCAGGGGCCGAGGGTGCCCAGCTGGAAGCCAGCAAAGCCTGGTCCAAAACCCTGATGGCTGAAGCCGGCGTGCCAACAG
CAAAACACTGGACAGTGAGCACAGCAGCAGACGCTCTGAGCGTGCTAAATCAAGTGCAACAACCCTTGGTTGTTAAGGCC
GATGGTTTAGCAGCAGGCAAAGGGGTCTGCGTGGCCGACAGCATCGAAGCAACCGCTGAAGCGATCCAGGAGGTCTTCAG
CGGCCGATTCGGAGCGGCCGGTTGCCAATTGGTGCTTGAAGAGCGACTAGAGGGGCCAGAAGTATCCGTCTTCGCACTCT
GCGATGGCGAGCGATTGTTATTGCTGCCTCCTGCTCAGGATCACAAACGTCTAGAGGACGGCGACAGGGGGCCTAACACC
GGGGGCATGGGGGCTTATGCCCCTGCACCCCTGCTCGATGCAGCAGGACTCGACCTTGTGCGCAGGGTTGTCCTTGAGCC
AACCCTGGCGACCCTGCAACAGCGAGGCATCGACTATCGAGGTGTCATCTATGCCGGATTGATGCTCACCCCCTCTGGTC
CGCAGGTGATTGAATTCAACTGTCGCTTCGGCGATCCCGAATGCCAGACCCTCATGCCACTGATGGGTCCAGAGCTGGCA
AAGGTTCTGCAGGCTTGTGCCCTTGGTTGCCTCGATCAAGCACCAGAGCTGTTGATCGTGGACCGCTGTAGTGCATGTGT
TGTGGCTGCAGCAGCGGGCTACCCCAATGCACCCCGCAAGGGTGACCGCCTCAACATTCAGCTACAGCCAGGCCCATCGC
TACAGCTTTTCCAGGCTGGCACCCGCTGCACTGAAGAGGGAGATCAACTCACCAATGGCGGCAGAGTGTTGGCGGTTGTG
GCTCAAGCAAACGATTTTGATCAGGCCTTCTCAAACGCCTACCAAGGTCTTGATCAGGTGTATTTCGAAGGGATCACTTA
CCGTCGGGACATCGGTCACCAAGTGCGCCAGTCCTAG

Upstream 100 bases:

>100_bases
GGAGTTGAGTCGTTCAACAAAGATGGAAGAGGCAACGGATTCCTCTGACGAGATCCGGCCTACTCAAACCTAGGGGTCTT
CCCCGTCCCTGCTTGTTCCT

Downstream 100 bases:

>100_bases
GGTGAAGAGACGCTGAATGGGTTGATGGCCAGCAGTACTTCGCAAGCCATCGCAGAGTGGGCTCCCCCAGGAGATGGCCA
ACCCCCACCAGAGCCACCCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MAMSSTRPHPLPPLERLLVVGSGGRENALAWALRRCSGIEDVWVTPGNGGTKEHQGCGCLAIAETDVNGLIDACQSLQID
LVVIGPEAPLAAGVADGLRKVGLAVFGPGAEGAQLEASKAWSKTLMAEAGVPTAKHWTVSTAADALSVLNQVQQPLVVKA
DGLAAGKGVCVADSIEATAEAIQEVFSGRFGAAGCQLVLEERLEGPEVSVFALCDGERLLLLPPAQDHKRLEDGDRGPNT
GGMGAYAPAPLLDAAGLDLVRRVVLEPTLATLQQRGIDYRGVIYAGLMLTPSGPQVIEFNCRFGDPECQTLMPLMGPELA
KVLQACALGCLDQAPELLIVDRCSACVVAAAAGYPNAPRKGDRLNIQLQPGPSLQLFQAGTRCTEEGDQLTNGGRVLAVV
AQANDFDQAFSNAYQGLDQVYFEGITYRRDIGHQVRQS

Sequences:

>Translated_438_residues
MAMSSTRPHPLPPLERLLVVGSGGRENALAWALRRCSGIEDVWVTPGNGGTKEHQGCGCLAIAETDVNGLIDACQSLQID
LVVIGPEAPLAAGVADGLRKVGLAVFGPGAEGAQLEASKAWSKTLMAEAGVPTAKHWTVSTAADALSVLNQVQQPLVVKA
DGLAAGKGVCVADSIEATAEAIQEVFSGRFGAAGCQLVLEERLEGPEVSVFALCDGERLLLLPPAQDHKRLEDGDRGPNT
GGMGAYAPAPLLDAAGLDLVRRVVLEPTLATLQQRGIDYRGVIYAGLMLTPSGPQVIEFNCRFGDPECQTLMPLMGPELA
KVLQACALGCLDQAPELLIVDRCSACVVAAAAGYPNAPRKGDRLNIQLQPGPSLQLFQAGTRCTEEGDQLTNGGRVLAVV
AQANDFDQAFSNAYQGLDQVYFEGITYRRDIGHQVRQS
>Mature_437_residues
AMSSTRPHPLPPLERLLVVGSGGRENALAWALRRCSGIEDVWVTPGNGGTKEHQGCGCLAIAETDVNGLIDACQSLQIDL
VVIGPEAPLAAGVADGLRKVGLAVFGPGAEGAQLEASKAWSKTLMAEAGVPTAKHWTVSTAADALSVLNQVQQPLVVKAD
GLAAGKGVCVADSIEATAEAIQEVFSGRFGAAGCQLVLEERLEGPEVSVFALCDGERLLLLPPAQDHKRLEDGDRGPNTG
GMGAYAPAPLLDAAGLDLVRRVVLEPTLATLQQRGIDYRGVIYAGLMLTPSGPQVIEFNCRFGDPECQTLMPLMGPELAK
VLQACALGCLDQAPELLIVDRCSACVVAAAAGYPNAPRKGDRLNIQLQPGPSLQLFQAGTRCTEEGDQLTNGGRVLAVVA
QANDFDQAFSNAYQGLDQVYFEGITYRRDIGHQVRQS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=44.6009389671362, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=44.6009389671362, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=44.6009389671362, Blast_Score=346, Evalue=3e-95,
Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=44.6009389671362, Blast_Score=345, Evalue=3e-95,
Organism=Escherichia coli, GI1790438, Length=421, Percent_Identity=42.2802850356295, Blast_Score=319, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=39.8126463700234, Blast_Score=284, Evalue=6e-77,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=38.6363636363636, Blast_Score=299, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24582400, Length=428, Percent_Identity=41.8224299065421, Blast_Score=301, Evalue=9e-82,
Organism=Drosophila melanogaster, GI62471619, Length=428, Percent_Identity=41.8224299065421, Blast_Score=300, Evalue=1e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46025; Mature: 45894

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSSTRPHPLPPLERLLVVGSGGRENALAWALRRCSGIEDVWVTPGNGGTKEHQGCGCL
CCCCCCCCCCCCHHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEE
AIAETDVNGLIDACQSLQIDLVVIGPEAPLAAGVADGLRKVGLAVFGPGAEGAQLEASKA
EEECCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCEECHHHH
WSKTLMAEAGVPTAKHWTVSTAADALSVLNQVQQPLVVKADGLAAGKGVCVADSIEATAE
HHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECHHHHHHH
AIQEVFSGRFGAAGCQLVLEERLEGPEVSVFALCDGERLLLLPPAQDHKRLEDGDRGPNT
HHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHCCCCCCCCCC
GGMGAYAPAPLLDAAGLDLVRRVVLEPTLATLQQRGIDYRGVIYAGLMLTPSGPQVIEFN
CCCCCCCCCCHHHHCCHHHHHHHHHCHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEE
CRFGDPECQTLMPLMGPELAKVLQACALGCLDQAPELLIVDRCSACVVAAAAGYPNAPRK
ECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCC
GDRLNIQLQPGPSLQLFQAGTRCTEEGDQLTNGGRVLAVVAQANDFDQAFSNAYQGLDQV
CCEEEEEECCCCCEEHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
YFEGITYRRDIGHQVRQS
HHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AMSSTRPHPLPPLERLLVVGSGGRENALAWALRRCSGIEDVWVTPGNGGTKEHQGCGCL
CCCCCCCCCCCHHHHEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCEE
AIAETDVNGLIDACQSLQIDLVVIGPEAPLAAGVADGLRKVGLAVFGPGAEGAQLEASKA
EEECCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCCCEECHHHH
WSKTLMAEAGVPTAKHWTVSTAADALSVLNQVQQPLVVKADGLAAGKGVCVADSIEATAE
HHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEECHHHHHHH
AIQEVFSGRFGAAGCQLVLEERLEGPEVSVFALCDGERLLLLPPAQDHKRLEDGDRGPNT
HHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCHHHHCCCCCCCCCC
GGMGAYAPAPLLDAAGLDLVRRVVLEPTLATLQQRGIDYRGVIYAGLMLTPSGPQVIEFN
CCCCCCCCCCHHHHCCHHHHHHHHHCHHHHHHHHCCCCCCEEEEEEEEECCCCCCEEEEE
CRFGDPECQTLMPLMGPELAKVLQACALGCLDQAPELLIVDRCSACVVAAAAGYPNAPRK
ECCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCCCCCCC
GDRLNIQLQPGPSLQLFQAGTRCTEEGDQLTNGGRVLAVVAQANDFDQAFSNAYQGLDQV
CCEEEEEECCCCCEEHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHH
YFEGITYRRDIGHQVRQS
HHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]