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The map label for this gene is aroK

Identifier: 124021918

GI number: 124021918

Start: 224035

End: 224613

Strand: Reverse

Name: aroK

Synonym: P9303_02051

Alternate gene names: 124021918

Gene position: 224613-224035 (Counterclockwise)

Preceding gene: 124021926

Following gene: 124021917

Centisome position: 8.37

GC content: 50.6

Gene sequence:

>579_bases
ATGAACGAAAGCCCAGCCCCTCATCCTCTCAAGCAAAGGCTTGGCGGTCGCAACCTCTATTTGGTGGGAATGATGGCCAG
TGGCAAGAGCAGCACAGGCAGGCCTCTAGCCGAACAACTCAGCTATGGGTTTGTGGATACAGATGCCGTCATCGAACAAC
TTGCTGGCCAACCAATCCCGAAGATCTTTAACGAAGAAGGAGAAGAAGGATTTCGAGCAATGGAGTCTCAAGTACTCAAT
GCCATTGGCCAACGCCATTCCCTTGTTGTTGCTACTGGCGGGGGGATGGTGAGTAAGCCAGAAAACTGGGGGATCTTGCA
TCAGGGCATCGTGATCTGGCTAAACCCTGGCCGAGAAGAACTATTCAGACGTTTACATGCTGATAGCGGCAATCGGCCAT
TACTACAAACAGAAGATCCAGAAGCAGCCTTCGACTGTTTATTTGCCGAACGCTTACCGCTTTATGCCGAAGCAGACTTG
CGTGTGGAAGTGGGAGCAGAGGAACCAGACGGGATTGCGCTAAAGATTATTGAGTTACTGCCCCAGCTGTTATCGCCCCC
TGTAAAAATGAATGGTTAA

Upstream 100 bases:

>100_bases
ACAGCCGCGGCCATGACCATGGCGTTCCAAATCGGGGACGTCAGTCATCGCTCTCTCGGACTAGAAGTCATCCTAGAGAT
CTATAGACATTTACGAACCA

Downstream 100 bases:

>100_bases
AGCAACTATTTTGTCAATACTAAAAGAATAGACCATTTAAGTCCGGAGGGTTGGCCTGATAGCCAATCCCAATCAAAACA
AACGATCCATCAATGACTTG

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MNESPAPHPLKQRLGGRNLYLVGMMASGKSSTGRPLAEQLSYGFVDTDAVIEQLAGQPIPKIFNEEGEEGFRAMESQVLN
AIGQRHSLVVATGGGMVSKPENWGILHQGIVIWLNPGREELFRRLHADSGNRPLLQTEDPEAAFDCLFAERLPLYAEADL
RVEVGAEEPDGIALKIIELLPQLLSPPVKMNG

Sequences:

>Translated_192_residues
MNESPAPHPLKQRLGGRNLYLVGMMASGKSSTGRPLAEQLSYGFVDTDAVIEQLAGQPIPKIFNEEGEEGFRAMESQVLN
AIGQRHSLVVATGGGMVSKPENWGILHQGIVIWLNPGREELFRRLHADSGNRPLLQTEDPEAAFDCLFAERLPLYAEADL
RVEVGAEEPDGIALKIIELLPQLLSPPVKMNG
>Mature_192_residues
MNESPAPHPLKQRLGGRNLYLVGMMASGKSSTGRPLAEQLSYGFVDTDAVIEQLAGQPIPKIFNEEGEEGFRAMESQVLN
AIGQRHSLVVATGGGMVSKPENWGILHQGIVIWLNPGREELFRRLHADSGNRPLLQTEDPEAAFDCLFAERLPLYAEADL
RVEVGAEEPDGIALKIIELLPQLLSPPVKMNG

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=167, Percent_Identity=39.5209580838323, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1786587, Length=170, Percent_Identity=30.5882352941176, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_PROM3 (A2C650)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016225.1
- ProteinModelPortal:   A2C650
- SMR:   A2C650
- STRING:   A2C650
- GeneID:   4777357
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_02051
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK00131
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 20962; Mature: 20962

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 48-48 BINDING 72-72 BINDING 94-94 BINDING 132-132 BINDING 151-151

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNESPAPHPLKQRLGGRNLYLVGMMASGKSSTGRPLAEQLSYGFVDTDAVIEQLAGQPIP
CCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCH
KIFNEEGEEGFRAMESQVLNAIGQRHSLVVATGGGMVSKPENWGILHQGIVIWLNPGREE
HHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCEEEEECCCHHH
LFRRLHADSGNRPLLQTEDPEAAFDCLFAERLPLYAEADLRVEVGAEEPDGIALKIIELL
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEECCCCCCCHHHHHHHHH
PQLLSPPVKMNG
HHHHCCCCCCCC
>Mature Secondary Structure
MNESPAPHPLKQRLGGRNLYLVGMMASGKSSTGRPLAEQLSYGFVDTDAVIEQLAGQPIP
CCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCH
KIFNEEGEEGFRAMESQVLNAIGQRHSLVVATGGGMVSKPENWGILHQGIVIWLNPGREE
HHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCEEEEECCCHHH
LFRRLHADSGNRPLLQTEDPEAAFDCLFAERLPLYAEADLRVEVGAEEPDGIALKIIELL
HHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCEEEEECCCCCCCHHHHHHHHH
PQLLSPPVKMNG
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA