Click here to switch to the map view.

The map label for this gene is ptmA [H]

Identifier: 124021827

GI number: 124021827

Start: 114258

End: 115040

Strand: Reverse

Name: ptmA [H]

Synonym: P9303_01141

Alternate gene names: 124021827

Gene position: 115040-114258 (Counterclockwise)

Preceding gene: 124021828

Following gene: 124021826

Centisome position: 4.29

GC content: 36.53

Gene sequence:

>783_bases
ATGGATAAGTCTCTAAGTCTGAAGGATGAATGTATTTTAATAACAGGAGCGGCAGGAAGAATAGGTTCAGCTGTCGCTAA
AATTGCTTTTCACGCGGGAGCAAATATAATACTAACCGATATAAATTATAAGGCTATGAATGATAGTGCAAAAGAATTGA
TAAAAATAGATGGCAGCAGGGTCCATTGTCTTGAGTGCGATATTACAGTAGCAGAGGATATTGAAAAAATGATAAGCTCG
TGCTTATCTGTTCATAGTGTTGTTAATGGGGCAGTGCACACTGCATATCCAACGTCTAGAGGATGGGGAGCAAGATTTGA
AGACATTAAACCTGAAGATTTATATTTAGACCTCAATATGCAGCTAGGGGGATCAATACTATTTTCACAGAAAATATTAA
AATGCTTTGAAAGGCAAAAGAAGGGTAGCTTAATTCTTGTATCTTCTATTCAAGGCATTAATGCGCCAAAGTTTGAGCAT
TATGAAGGCACTTCTATGTATTCTCCAATAGAATATGCCGCGATTAAAGCTGGTATTATTTCCATCACCAGGTGGCTTGC
AAAATATTATTCGGATAAAGGTATTCGTGTGAATTGTGTCAGTCCTGGCGGAATAAAAGATAATCAGCCTGTTGCATTCC
AACAGAAATATAAAAAGAGTTGTACTAATATCGGGTTGTTAGAGAGTGAAGATGTAGCTCATACTATAATATTCTTATTA
TCCAGTGCAGCATTTGCAATCAATGGCCAGAATATTATTATTGACGATGGATGGGTACTTTAA

Upstream 100 bases:

>100_bases
GTACTAAAAGAACACGCAATTGATATAGACACTGAATTTGATTTTGCTATGGCGAAATTTATATTTGAAGAATATCGTCC
CTCGTTAAAGGTTAGAGAAG

Downstream 100 bases:

>100_bases
TGACTAAGCAATCGTTAGCCTTGGTCACCGGTGCTGATGGCTTTATTGGATCACATTTGGTTGAATATCTATTAAATAAA
GGTTGCAATGTAAGGGCTTT

Product: flagellin modification protein A

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MDKSLSLKDECILITGAAGRIGSAVAKIAFHAGANIILTDINYKAMNDSAKELIKIDGSRVHCLECDITVAEDIEKMISS
CLSVHSVVNGAVHTAYPTSRGWGARFEDIKPEDLYLDLNMQLGGSILFSQKILKCFERQKKGSLILVSSIQGINAPKFEH
YEGTSMYSPIEYAAIKAGIISITRWLAKYYSDKGIRVNCVSPGGIKDNQPVAFQQKYKKSCTNIGLLESEDVAHTIIFLL
SSAAFAINGQNIIIDDGWVL

Sequences:

>Translated_260_residues
MDKSLSLKDECILITGAAGRIGSAVAKIAFHAGANIILTDINYKAMNDSAKELIKIDGSRVHCLECDITVAEDIEKMISS
CLSVHSVVNGAVHTAYPTSRGWGARFEDIKPEDLYLDLNMQLGGSILFSQKILKCFERQKKGSLILVSSIQGINAPKFEH
YEGTSMYSPIEYAAIKAGIISITRWLAKYYSDKGIRVNCVSPGGIKDNQPVAFQQKYKKSCTNIGLLESEDVAHTIIFLL
SSAAFAINGQNIIIDDGWVL
>Mature_260_residues
MDKSLSLKDECILITGAAGRIGSAVAKIAFHAGANIILTDINYKAMNDSAKELIKIDGSRVHCLECDITVAEDIEKMISS
CLSVHSVVNGAVHTAYPTSRGWGARFEDIKPEDLYLDLNMQLGGSILFSQKILKCFERQKKGSLILVSSIQGINAPKFEH
YEGTSMYSPIEYAAIKAGIISITRWLAKYYSDKGIRVNCVSPGGIKDNQPVAFQQKYKKSCTNIGLLESEDVAHTIIFLL
SSAAFAINGQNIIIDDGWVL

Specific function: Required for biosynthesis of LAH modification in the post-translational modification of Campylobacter coli flagellin [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=251, Percent_Identity=28.2868525896414, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI19923817, Length=269, Percent_Identity=24.5353159851301, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI32483357, Length=271, Percent_Identity=25.4612546125461, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI5031737, Length=274, Percent_Identity=27.007299270073, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1787905, Length=261, Percent_Identity=25.6704980842912, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789208, Length=262, Percent_Identity=22.9007633587786, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787335, Length=260, Percent_Identity=22.6923076923077, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17562904, Length=276, Percent_Identity=27.536231884058, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24639444, Length=259, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21355319, Length=263, Percent_Identity=28.5171102661597, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI23397609, Length=263, Percent_Identity=25.8555133079848, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357041, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 28410; Mature: 28410

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLSLKDECILITGAAGRIGSAVAKIAFHAGANIILTDINYKAMNDSAKELIKIDGSR
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCEECCHHHHHHHEECCCE
VHCLECDITVAEDIEKMISSCLSVHSVVNGAVHTAYPTSRGWGARFEDIKPEDLYLDLNM
EEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEC
QLGGSILFSQKILKCFERQKKGSLILVSSIQGINAPKFEHYEGTSMYSPIEYAAIKAGII
CCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SITRWLAKYYSDKGIRVNCVSPGGIKDNQPVAFQQKYKKSCTNIGLLESEDVAHTIIFLL
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SSAAFAINGQNIIIDDGWVL
HCCEEEECCCEEEECCCCCC
>Mature Secondary Structure
MDKSLSLKDECILITGAAGRIGSAVAKIAFHAGANIILTDINYKAMNDSAKELIKIDGSR
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCEECCHHHHHHHEECCCE
VHCLECDITVAEDIEKMISSCLSVHSVVNGAVHTAYPTSRGWGARFEDIKPEDLYLDLNM
EEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEC
QLGGSILFSQKILKCFERQKKGSLILVSSIQGINAPKFEHYEGTSMYSPIEYAAIKAGII
CCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
SITRWLAKYYSDKGIRVNCVSPGGIKDNQPVAFQQKYKKSCTNIGLLESEDVAHTIIFLL
HHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SSAAFAINGQNIIIDDGWVL
HCCEEEECCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8825781 [H]