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The map label for this gene is trmD [H]

Identifier: 123969011

GI number: 123969011

Start: 1259819

End: 1261045

Strand: Reverse

Name: trmD [H]

Synonym: A9601_14791

Alternate gene names: 123969011

Gene position: 1261045-1259819 (Counterclockwise)

Preceding gene: 123969012

Following gene: 123969010

Centisome position: 75.52

GC content: 32.36

Gene sequence:

>1227_bases
ATGAGTGGTTTTAATTTTGATGTAATTACATTGTTCCCTAAAGCTTTTGAATTAATAAATAATTTAGGGGTCATAACAAG
AGCTCTAGATAAGAAGTTAATCGATGTAAATTTACATGATTTAAGAGAATATGGAGAAGGTTCTTACAGACAAGTAGACG
ATAAGCCTTATGGAGGAGGAGCAGGTATGGTATTAAAACCTGAACCTATTTATAAGGCATATGAATCAATTAGAAAATCA
TCTAAAAGTAAAACTTTGTTGATGACCCCTCAGGGTAAAATTTTAAAGCAGAAGGATCTTGCGAGATGGTCCACTTTGGA
TCAAATAATAATTATTTGTGGTCAATATGAAGGTTTTGATGAAAGGATTAGATGTTTAGCTGATGAAGAGATATCGTTAG
GAGATTATGTCCTTTCTGGAGGAGAAATACCCGCTATATCAATAATTAATGGTTTGACTAGGTTATTACCAGGGACTCTT
GGTGATCCAGACTCCTTAATCGATGAGAGTCATAATTCTCCTTTATTAGAATATCCTCAATACACAAGGCCTTTAACTTT
TAAAGATATGAAAGTGCCAGATATTTTAGTGAGCGGTAATCATGAAGAGATTAAATCGTGGAGAAGAAAAAAAAGTTTCG
AAAGAACATTGGAGAGAAGAAGTGACTTAATTTCAAATGAAAACTACAAAAAATCCCCACAAAGTAAGAGAATAATAAAA
GAAAATAATCAATTCATGAAATTTAGAATAGGTAATGGATACGATATTCACAGACTAGTAGAGGATAGAGATTTAATTAT
TGGAGGTGTAAAATTGAATCATCCTGAAAATTTAGGATTGGATGGTCATAGTGATGCTGATGTTTTAAGTCACTCAATAA
TGGATGCATTATTAGGAGCGCTTTCGTTGGGCGACATAGGAAAGTATTTCCCCCCATCTGATGAAAAATGGAAAAATGCT
GATAGCTTGTTTTTGTTGTCAAAAGTAATTGACTTGATAAGACAAGATGGTTGGGAAATAAATAATATTGATAGTGTTCT
AGTTGCTGAAAGGCCAAAAATCATGCCACATGTAAAACTAATGAAAAAAAACATTTCTGAAATTTTAAATATTGATGAAA
ATTTAATTGGCATTAAAGCAACCACTAATGAAAAATTGGGTCCAGAAGGTAGAGAAGAGGGTATAAGTTGCCATTCAGTA
GTTCTTCTTGAGAAAAATGAGTTTTAA

Upstream 100 bases:

>100_bases
TGATGGTCCAGTTCACCTAGAGTTGTTTGTGAAAGTTGTTCCTAATTGGAGAAAAAAAGAATCAAGGTTAATTGAGTTTG
GTTATGAGGAAGAATTCTAG

Downstream 100 bases:

>100_bases
TTTAAATTTGAAAAAAAATTTTCAAAGATTTTGTTTATTATTAATTTGTTCAGTAGTTTTAATTTTTCAATCTGAATTTC
CCAATTTAAAAGCTCTTACA

Product: putative bifunctional enzyme: tRNA methyltransferase; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSGFNFDVITLFPKAFELINNLGVITRALDKKLIDVNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPEPIYKAYESIRKS
SKSKTLLMTPQGKILKQKDLARWSTLDQIIIICGQYEGFDERIRCLADEEISLGDYVLSGGEIPAISIINGLTRLLPGTL
GDPDSLIDESHNSPLLEYPQYTRPLTFKDMKVPDILVSGNHEEIKSWRRKKSFERTLERRSDLISNENYKKSPQSKRIIK
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLNHPENLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDEKWKNA
DSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHVKLMKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSV
VLLEKNEF

Sequences:

>Translated_408_residues
MSGFNFDVITLFPKAFELINNLGVITRALDKKLIDVNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPEPIYKAYESIRKS
SKSKTLLMTPQGKILKQKDLARWSTLDQIIIICGQYEGFDERIRCLADEEISLGDYVLSGGEIPAISIINGLTRLLPGTL
GDPDSLIDESHNSPLLEYPQYTRPLTFKDMKVPDILVSGNHEEIKSWRRKKSFERTLERRSDLISNENYKKSPQSKRIIK
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLNHPENLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDEKWKNA
DSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHVKLMKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSV
VLLEKNEF
>Mature_407_residues
SGFNFDVITLFPKAFELINNLGVITRALDKKLIDVNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPEPIYKAYESIRKSS
KSKTLLMTPQGKILKQKDLARWSTLDQIIIICGQYEGFDERIRCLADEEISLGDYVLSGGEIPAISIINGLTRLLPGTLG
DPDSLIDESHNSPLLEYPQYTRPLTFKDMKVPDILVSGNHEEIKSWRRKKSFERTLERRSDLISNENYKKSPQSKRIIKE
NNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLNHPENLGLDGHSDADVLSHSIMDALLGALSLGDIGKYFPPSDEKWKNAD
SLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHVKLMKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSVV
LLEKNEF

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=237, Percent_Identity=39.662447257384, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=45.5696202531646, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 46103; Mature: 45972

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFNFDVITLFPKAFELINNLGVITRALDKKLIDVNLHDLREYGEGSYRQVDDKPYGGG
CCCCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCCCCCC
AGMVLKPEPIYKAYESIRKSSKSKTLLMTPQGKILKQKDLARWSTLDQIIIICGQYEGFD
CCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCHH
ERIRCLADEEISLGDYVLSGGEIPAISIINGLTRLLPGTLGDPDSLIDESHNSPLLEYPQ
HHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
YTRPLTFKDMKVPDILVSGNHEEIKSWRRKKSFERTLERRSDLISNENYKKSPQSKRIIK
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLNHPENLGLDGHSDADVLSHSIMDALLGA
CCCCEEEEECCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDEKWKNADSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHVKL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHH
MKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSVVLLEKNEF
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCC
>Mature Secondary Structure 
SGFNFDVITLFPKAFELINNLGVITRALDKKLIDVNLHDLREYGEGSYRQVDDKPYGGG
CCCCEEHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEECCCCCCCCC
AGMVLKPEPIYKAYESIRKSSKSKTLLMTPQGKILKQKDLARWSTLDQIIIICGQYEGFD
CCEEECCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCHH
ERIRCLADEEISLGDYVLSGGEIPAISIINGLTRLLPGTLGDPDSLIDESHNSPLLEYPQ
HHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCC
YTRPLTFKDMKVPDILVSGNHEEIKSWRRKKSFERTLERRSDLISNENYKKSPQSKRIIK
CCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
ENNQFMKFRIGNGYDIHRLVEDRDLIIGGVKLNHPENLGLDGHSDADVLSHSIMDALLGA
CCCCEEEEECCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDEKWKNADSLFLLSKVIDLIRQDGWEINNIDSVLVAERPKIMPHVKL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHH
MKKNISEILNIDENLIGIKATTNEKLGPEGREEGISCHSVVLLEKNEF
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA