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The map label for this gene is bioB [H]

Identifier: 123968732

GI number: 123968732

Start: 1015926

End: 1016933

Strand: Direct

Name: bioB [H]

Synonym: A9601_11991

Alternate gene names: 123968732

Gene position: 1015926-1016933 (Clockwise)

Preceding gene: 123968731

Following gene: 123968733

Centisome position: 60.84

GC content: 35.12

Gene sequence:

>1008_bases
ATGATAAATTCGAATAATCAGGTCTTAAGTGAAATTAGGTTCGATTGGAATAAAAAGGAGATATTAGAAATTCTTAATAT
GCCTCTGATCGATTTAATGTGGGAATCACAAATCGTTCACAGGAAATTCAACAGCTATAACATTCAATTAGCATCATTGT
TCAGCGTAAAAACTGGTGGATGTGAGGAAAATTGTTCATATTGTAGCCAATCAATTTATAGTGCTAGCGAAATAAAAAGT
CATCCACAATTTCAAGTTGAAGAGGTTTTAGCAAGAGCTAAAATAGCAAAAAATGAGGGTGCAGATAGGTTTTGTATGGG
TTGGGCATGGAGAGAAATTAGAGATGGAAAATCTTTTAATGCAATGTTAGAGATGGTTAGCGGTGTAAGAGATTTAGGAA
TGGAAGCATGCGTTACTGCTGGGATGCTTACAGAAGAACAAGCTTCCCGACTGGCTGATGCAGGTTTAACAGCGTACAAC
CACAATCTTGATACTAGCCCTGAGCATTATAAAAATATTATTACGACAAGAACTTATCAAGACAGACTAGATACTATCAA
AAGAGTGAGAAATGCAGGAATAAATGTTTGTTGTGGAGGAATAATAGGCTTGGGTGAGACTAATGGCGATAGAGCATCTC
TTTTGGAAGTGCTTTCAAACATGAATCCACACCCTGAAAGTGTTCCAATAAATTCATTAGTAGCTATTGAAGGTACTGGT
TTAGAAGATAATCAAGAAATTGATTCTATTGAGATGATAAGGATGATAGCTACAGCCAGAATTCTTATGCCTAAAAGTAA
AATAAGATTAAGTGCAGGAAGAGAAAAGCTCTCAAAAGAAGCCCAAATCTTATGTTTTCAATGCGGTGCAAATTCCATTT
TTTACGGAGATGAGTTACTCACTACTTCAAATCCATCTTTTCAGTCAGACAGAAAACTTCTCAAAGAGGTTGGAGTATCA
TTTAACAAAGATTTTGAAACTTGTGAAAAAACATTATCTTCTTTATGA

Upstream 100 bases:

>100_bases
AAGCAATCATTGATTACCAATCGAGGAATAGACGTTTCGGAGGTATAGAATCATTACCAAATGAATCTTTTGAAGATTCT
CATTATACTCTCTAAAAATT

Downstream 100 bases:

>100_bases
AAGGCAAAAATTATAAAATTACTTCTCTATACTCTTTCTTCCCATTTCAAGAAAACTTAATTTTTGATCTCAAAAATAAA
TTATTAGAAATCGAAAATGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MINSNNQVLSEIRFDWNKKEILEILNMPLIDLMWESQIVHRKFNSYNIQLASLFSVKTGGCEENCSYCSQSIYSASEIKS
HPQFQVEEVLARAKIAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYN
HNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVS
FNKDFETCEKTLSSL

Sequences:

>Translated_335_residues
MINSNNQVLSEIRFDWNKKEILEILNMPLIDLMWESQIVHRKFNSYNIQLASLFSVKTGGCEENCSYCSQSIYSASEIKS
HPQFQVEEVLARAKIAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYN
HNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVS
FNKDFETCEKTLSSL
>Mature_335_residues
MINSNNQVLSEIRFDWNKKEILEILNMPLIDLMWESQIVHRKFNSYNIQLASLFSVKTGGCEENCSYCSQSIYSASEIKS
HPQFQVEEVLARAKIAKNEGADRFCMGWAWREIRDGKSFNAMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYN
HNLDTSPEHYKNIITTRTYQDRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELLTTSNPSFQSDRKLLKEVGVS
FNKDFETCEKTLSSL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=309, Percent_Identity=51.4563106796116, Blast_Score=340, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6321725, Length=326, Percent_Identity=45.7055214723926, Blast_Score=308, Evalue=7e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 37473; Mature: 37473

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSNNQVLSEIRFDWNKKEILEILNMPLIDLMWESQIVHRKFNSYNIQLASLFSVKTGG
CCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
CEENCSYCSQSIYSASEIKSHPQFQVEEVLARAKIAKNEGADRFCMGWAWREIRDGKSFN
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
AMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQ
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCHHH
DRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
HHHHHHHHHHHCCCCEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEECCCCCEEECCEEE
TTSNPSFQSDRKLLKEVGVSFNKDFETCEKTLSSL
ECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MINSNNQVLSEIRFDWNKKEILEILNMPLIDLMWESQIVHRKFNSYNIQLASLFSVKTGG
CCCCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
CEENCSYCSQSIYSASEIKSHPQFQVEEVLARAKIAKNEGADRFCMGWAWREIRDGKSFN
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHH
AMLEMVSGVRDLGMEACVTAGMLTEEQASRLADAGLTAYNHNLDTSPEHYKNIITTRTYQ
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHCCHHH
DRLDTIKRVRNAGINVCCGGIIGLGETNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
HHHHHHHHHHHCCCCEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
LEDNQEIDSIEMIRMIATARILMPKSKIRLSAGREKLSKEAQILCFQCGANSIFYGDELL
CCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHEEEEECCCCCEEECCEEE
TTSNPSFQSDRKLLKEVGVSFNKDFETCEKTLSSL
ECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA