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The map label for this gene is folD [H]

Identifier: 123968707

GI number: 123968707

Start: 989255

End: 990151

Strand: Direct

Name: folD [H]

Synonym: A9601_11741

Alternate gene names: 123968707

Gene position: 989255-990151 (Clockwise)

Preceding gene: 123968705

Following gene: 123968708

Centisome position: 59.24

GC content: 32.22

Gene sequence:

>897_bases
ATGTCATTAAAATTAGACGGTAAAAAATTATCTCTTGAAATTGAAAAAAGATTAAATGACTACATTTCTAATAATAAAAT
AATTGCAAAAAGAGTGCCCGGTTTAGCTGTAATAAGAATAGGTGAAGACCCTGCGAGTGGTGTTTACGTTAATAACAAGG
AAAAGGCATGTTCAAGGATTGGAATAAAGAGCTTTATCTTTCATTTAGATGAAAGTGTAGAGCAAAAAGAAGTTGAACAA
TTAATAATCAAACTGAATTCGGATAAGGATATTGATGGAATGTTGCTACAACTTCCCATCCCAAAGAAGTTTGATGAGCA
AAAACTGATCAGCCATATTAATCCAAGCAAAGATGTAGATGGATTAAATGAGATAAACATCGGTAAATTAGTAAAAAATG
AGCCTGGGATGAGATCATGTACACCCGCAGGAATTATTAATTTATTAAGATCCCAAAATATTACAATTGAAGGTAAGAAA
ATTGTTGTTATTGGAAGAAGTTTGCTTGTTGGGAAACCTCTATCGCTTATGTTATTGAATCTAAATGGCACTGTAACAAT
GACCCATTCAAAAACATTAAATTTGAATAAAGTCTGTAGAGAAGCCGACATATTAATTGCGGCTGCAGGAAAACCCAATC
TTATAGATTCGAGTTTTGTTAAAGAAGGAGCCGTAATTATTGATGTTGGAATACATAGATTAAAAAGTTCCGATAAAAAT
CAAACCAGATTATGTGGAGATGTATTATTAGAAGATGTCATTTCTAAAGTATTTGCTTACACACCTGTACCAGGAGGTGT
TGGACCAATGACAGTAACAATGTTACTTGTAAATACTATTTTTAGCTGGCAAAAACAATTTGGTTTATCATCAACTCTTA
ATGACCTTTTGCCATAA

Upstream 100 bases:

>100_bases
TTTAGGGAAGTTGTGATGTTTTGCACAGAATATAAATAAATCTATTTGAATAATTTAAAATTATAGGCATGCTAGGTGTA
AAAAGGAGATGTTTTTCTAA

Downstream 100 bases:

>100_bases
ACTCCAAGATGCAAAATTTTTTTACTAACGGTATAAAATGACTGAAGTTATAAATAATATTTCTGATTTTGAAAAATATC
TTAAAAACACAAAAAAGGTT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSLKLDGKKLSLEIEKRLNDYISNNKIIAKRVPGLAVIRIGEDPASGVYVNNKEKACSRIGIKSFIFHLDESVEQKEVEQ
LIIKLNSDKDIDGMLLQLPIPKKFDEQKLISHINPSKDVDGLNEINIGKLVKNEPGMRSCTPAGIINLLRSQNITIEGKK
IVVIGRSLLVGKPLSLMLLNLNGTVTMTHSKTLNLNKVCREADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
QTRLCGDVLLEDVISKVFAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP

Sequences:

>Translated_298_residues
MSLKLDGKKLSLEIEKRLNDYISNNKIIAKRVPGLAVIRIGEDPASGVYVNNKEKACSRIGIKSFIFHLDESVEQKEVEQ
LIIKLNSDKDIDGMLLQLPIPKKFDEQKLISHINPSKDVDGLNEINIGKLVKNEPGMRSCTPAGIINLLRSQNITIEGKK
IVVIGRSLLVGKPLSLMLLNLNGTVTMTHSKTLNLNKVCREADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
QTRLCGDVLLEDVISKVFAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP
>Mature_297_residues
SLKLDGKKLSLEIEKRLNDYISNNKIIAKRVPGLAVIRIGEDPASGVYVNNKEKACSRIGIKSFIFHLDESVEQKEVEQL
IIKLNSDKDIDGMLLQLPIPKKFDEQKLISHINPSKDVDGLNEINIGKLVKNEPGMRSCTPAGIINLLRSQNITIEGKKI
VVIGRSLLVGKPLSLMLLNLNGTVTMTHSKTLNLNKVCREADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKNQ
TRLCGDVLLEDVISKVFAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=40.5405405405405, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=37.9310344827586, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=39.3835616438356, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI36796743, Length=200, Percent_Identity=29.5, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1786741, Length=276, Percent_Identity=43.1159420289855, Blast_Score=234, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17568735, Length=268, Percent_Identity=39.5522388059701, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=40.7284768211921, Blast_Score=212, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6319558, Length=298, Percent_Identity=39.9328859060403, Blast_Score=209, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6322933, Length=282, Percent_Identity=26.5957446808511, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=40.0673400673401, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=40.0673400673401, Blast_Score=212, Evalue=2e-55,
Organism=Drosophila melanogaster, GI62472483, Length=283, Percent_Identity=37.4558303886926, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI45551871, Length=283, Percent_Identity=37.4558303886926, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=37.0629370629371, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=37.0629370629371, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 32687; Mature: 32556

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKLDGKKLSLEIEKRLNDYISNNKIIAKRVPGLAVIRIGEDPASGVYVNNKEKACSRI
CCEECCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCEEECCHHHHHHHH
GIKSFIFHLDESVEQKEVEQLIIKLNSDKDIDGMLLQLPIPKKFDEQKLISHINPSKDVD
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GLNEINIGKLVKNEPGMRSCTPAGIINLLRSQNITIEGKKIVVIGRSLLVGKPLSLMLLN
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEEECHHHHCCCCEEEEEE
LNGTVTMTHSKTLNLNKVCREADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
CCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEECHHHHCCCCCC
QTRLCGDVLLEDVISKVFAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure 
SLKLDGKKLSLEIEKRLNDYISNNKIIAKRVPGLAVIRIGEDPASGVYVNNKEKACSRI
CEECCCCEEEEEHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCEEECCHHHHHHHH
GIKSFIFHLDESVEQKEVEQLIIKLNSDKDIDGMLLQLPIPKKFDEQKLISHINPSKDVD
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCC
GLNEINIGKLVKNEPGMRSCTPAGIINLLRSQNITIEGKKIVVIGRSLLVGKPLSLMLLN
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCEEEEEECHHHHCCCCEEEEEE
LNGTVTMTHSKTLNLNKVCREADILIAAAGKPNLIDSSFVKEGAVIIDVGIHRLKSSDKN
CCCEEEEEECCCCCHHHHHHHCCEEEEECCCCCCCCHHHHHCCCEEEEECHHHHCCCCCC
QTRLCGDVLLEDVISKVFAYTPVPGGVGPMTVTMLLVNTIFSWQKQFGLSSTLNDLLP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA