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The map label for this gene is spt [H]

Identifier: 123968474

GI number: 123968474

Start: 810094

End: 811278

Strand: Direct

Name: spt [H]

Synonym: A9601_09411

Alternate gene names: 123968474

Gene position: 810094-811278 (Clockwise)

Preceding gene: 123968470

Following gene: 123968476

Centisome position: 48.51

GC content: 34.94

Gene sequence:

>1185_bases
TTGACAGAAGCAAAACTTATTTCAGCTTTAAATGAAGAGAATTTATCTCATTTCTCAAAGACTTATGTTCCCTCTAGACT
TTTATTAGGTCCTGGGCCTTCAAACGCACATCCAGAAGTCTTAAGCGCTCTTTCTTTGAATCCTATTGGTCACTTAGATG
AAGCATATATTTCATTGATGTCTGATGTTCAACAACTTCTAAGATATACCTGGCAATGTAATAATCGTCTGACTCTTCCA
ATGAGTGGTACTGGAAGTGCAGCTATGGAAGCATCAATAGCTAATTTTATAGAGGAAGGAGAAAAAATTCTTATCGCTAA
AAAAGGATATTTTGGAGACAGACTTGTTGATATGGCAACAAGATATAAAGCAGATGTATCTGTTATTGAAAAACCTTGGG
GTGAATCCTTTTCTTATGAAGAAATCAAGTATGAAATAGAAACTAAAAAACCAGCAATTTTTGCTATTGTCCATGCTGAA
ACATCAAGTGGTGTTTTACAACCTCTTGATGGTATAGGGGATGTATGTAGAAAAAATAACTGCTTGTTTTTAGTTGATGC
AGTTACTTCTCTTGGAGCTCTAGAACTATTGATTGACGAATGGAAAATTGATCTAGCATACAGTTGTAGTCAAAAGGGAT
TAAGTTGTCCCCCGGGATTAAGCCCTTTTACGATGAATAAAAGAGCTGAAGAAAAACTAAGTTCAAGAAAAACAAAAGTA
CCTAACTGGTATTTAGATTTATCTCTTTTAAATAAATATTGGGGTTCTGATCGCGTTTACCATCATACGGCCCCTGTAAA
TATGAATTTTGCTATTCGAGAAGGTTTGCGATTAATTGCGAATGAGGGTTTAGAAAATGTTTGGAATAGACACAATACTA
ATGCAAAGAAACTTTGGAATGGCCTGGAAAGTCTTGGCATGGAATTACATGTATCAGAGGATTATAGATTGCCAACCTTA
ACAACAGTTAAAATACCTCCAGCAGTTGATGGGGATGGTTTTAGAAATCATCTCTTAAGAAACTTTGGAATTGAAATAGG
AAATGGGCTTGGAGAATTATCTGGTAAGGTATGGCGGATAGGTTTAATGGGCTTTAACTCAAGTGATGAGAATGTCGACA
GATTATTAAACTTATTTGATACTGAGCTAAAGAAATTTTCTATTTTTGAGTCCTCAACTTTTTGA

Upstream 100 bases:

>100_bases
TAATTGTTTACCTAAATATTTAAATTGAATTAATTTCTATAAATAAATATCGCTTATGTGGAGATATTAGATTAATTTAA
AAGTAATTGAATGTAATGCT

Downstream 100 bases:

>100_bases
ACCAAATCTGTAAAATTTGACTACATTCATCTTCTAGGATACCTCCAATTATTTCCATTTTGTGATGAGCACTTTCATGT
TTTGATAGGTCAATTGAGCC

Product: serine:pyruvate/alanine:glyoxylate aminotransferase

Products: pyruvate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MTEAKLISALNEENLSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLMSDVQQLLRYTWQCNNRLTLP
MSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMATRYKADVSVIEKPWGESFSYEEIKYEIETKKPAIFAIVHAE
TSSGVLQPLDGIGDVCRKNNCLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEEKLSSRKTKV
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLENVWNRHNTNAKKLWNGLESLGMELHVSEDYRLPTL
TTVKIPPAVDGDGFRNHLLRNFGIEIGNGLGELSGKVWRIGLMGFNSSDENVDRLLNLFDTELKKFSIFESSTF

Sequences:

>Translated_394_residues
MTEAKLISALNEENLSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLMSDVQQLLRYTWQCNNRLTLP
MSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMATRYKADVSVIEKPWGESFSYEEIKYEIETKKPAIFAIVHAE
TSSGVLQPLDGIGDVCRKNNCLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEEKLSSRKTKV
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLENVWNRHNTNAKKLWNGLESLGMELHVSEDYRLPTL
TTVKIPPAVDGDGFRNHLLRNFGIEIGNGLGELSGKVWRIGLMGFNSSDENVDRLLNLFDTELKKFSIFESSTF
>Mature_393_residues
TEAKLISALNEENLSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLMSDVQQLLRYTWQCNNRLTLPM
SGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMATRYKADVSVIEKPWGESFSYEEIKYEIETKKPAIFAIVHAET
SSGVLQPLDGIGDVCRKNNCLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEEKLSSRKTKVP
NWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLENVWNRHNTNAKKLWNGLESLGMELHVSEDYRLPTLT
TVKIPPAVDGDGFRNHLLRNFGIEIGNGLGELSGKVWRIGLMGFNSSDENVDRLLNLFDTELKKFSIFESSTF

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=366, Percent_Identity=41.5300546448087, Blast_Score=302, Evalue=3e-82,
Organism=Caenorhabditis elegans, GI17536281, Length=365, Percent_Identity=40, Blast_Score=295, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6321079, Length=373, Percent_Identity=26.8096514745308, Blast_Score=120, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17530823, Length=368, Percent_Identity=40.2173913043478, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.44

Molecular weight: Translated: 44053; Mature: 43922

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAKLISALNEENLSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLM
CCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SDVQQLLRYTWQCNNRLTLPMSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMAT
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RYKADVSVIEKPWGESFSYEEIKYEIETKKPAIFAIVHAETSSGVLQPLDGIGDVCRKNN
HHHHHHHHHHCCCCCCCCHHHEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCC
CLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEEKLSSRKTKV
CEEEEEHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLENVWNRHNTNAKKLWN
CCEEEEHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHH
GLESLGMELHVSEDYRLPTLTTVKIPPAVDGDGFRNHLLRNFGIEIGNGLGELSGKVWRI
HHHHCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHCCCHHHCCCCEEEE
GLMGFNSSDENVDRLLNLFDTELKKFSIFESSTF
EEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
TEAKLISALNEENLSHFSKTYVPSRLLLGPGPSNAHPEVLSALSLNPIGHLDEAYISLM
CHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCCCCHHHHHHHHH
SDVQQLLRYTWQCNNRLTLPMSGTGSAAMEASIANFIEEGEKILIAKKGYFGDRLVDMAT
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
RYKADVSVIEKPWGESFSYEEIKYEIETKKPAIFAIVHAETSSGVLQPLDGIGDVCRKNN
HHHHHHHHHHCCCCCCCCHHHEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCC
CLFLVDAVTSLGALELLIDEWKIDLAYSCSQKGLSCPPGLSPFTMNKRAEEKLSSRKTKV
CEEEEEHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
PNWYLDLSLLNKYWGSDRVYHHTAPVNMNFAIREGLRLIANEGLENVWNRHNTNAKKLWN
CCEEEEHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHH
GLESLGMELHVSEDYRLPTLTTVKIPPAVDGDGFRNHLLRNFGIEIGNGLGELSGKVWRI
HHHHCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCHHCCCHHHCCCCEEEE
GLMGFNSSDENVDRLLNLFDTELKKFSIFESSTF
EEEECCCCCHHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-alanine; glyoxylate

Specific reaction: L-alanine + glyoxylate = pyruvate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]