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The map label for this gene is dapL

Identifier: 123966913

GI number: 123966913

Start: 1476473

End: 1477699

Strand: Reverse

Name: dapL

Synonym: P9515_16801

Alternate gene names: 123966913

Gene position: 1477699-1476473 (Counterclockwise)

Preceding gene: 123966916

Following gene: 123966912

Centisome position: 86.71

GC content: 33.01

Gene sequence:

>1227_bases
GTGGTACAAATAAACGAAAATTATTTAAAGCTCAAAGCAGGATATCTATTTCCTGAAATTTCAAAAAGAGTAAATAGTTA
TACACAAGCTAATCAAGGCTCAGAAGTTATTAAGCTTGGAATAGGAGATGTAACTGAACCTCTACCTAATGCTTGTATAA
ATGCGATGAGTAAAGCATTAAATGAAATGGGAACACATGAAGGATTTAAAGGTTATGGTCCAGAGCAAGGATATGAATGG
CTTAGAGAGAAAATTTCTAAAAATGATTTTATTTCAAGAGGATGTCAAATTACACCGGAAGAAATATTTGTCTCAGATGG
TTCAAAATGTGATAGCAGTAATATCTTAGATATTCTTGGCCACGATAATTTAATTGCCGTAACAGATCCTGTTTATCCTG
TTTATGTAGATAGTAATGTGATGACTGGGCGAACAGGAGAAACACTTAAAAATGGAACTTATCAAGGATTATTATATTTA
GCGATTAATGAGGATAATAATTTTTTACCTGAAATTCCTAAAAATAAAGTTGATATTGTTTATCTCTGTTTTCCAAATAA
TCCCACTGGGGCAACTATTACTAAAGATGAATTAAAAAAATGGGTTGACTATGCTAATCATAATAAGTCTTTGATTCTCT
TCGATGCAGCTTATGAAGCTTTTATTCAAGATAAAGATGTACCTCATTCAATATATGAGATAGATGGAGCAAAAAGTTGT
GCTATAGAATTTAGATCTTTCTCTAAAAATGCTGGATTTACCGGCGTAAGATGTGCTTATACAGTAATCCCTAAATGTTT
AACAGGACAAAATTCAAAAGGTGATAAGGTTGATTTATGGCCATTATGGAATAGGCGTCAATGTACTAAATTTAATGGAG
TCAGCTATGTCGTTCAAAAAGGAGCTGAAGCAGTGTATTCATCTCAAGGTAAAAAAGAGGTTAATTCTTTAATTGATTTT
TATATGGAAAATGCAAAGATTATGCGAAATAAGCTAAGAAGTGCTGGCTTTACAGTTTATGGCGGTTGTAACGCGCCATA
TGTTTGGATTAAAGTCCCAGCTGATATGACTTCTTGGGACTTTTTTGATCATTTACTTGAAAAAGCTAACGTAGTGGGAA
CACCTGGAAGTGGTTTTGGGTTGGCAGGAGAGGGTTATTTTCGATTATCTGCCTTTAACTCAAGATTAAATGTCTCTAAT
GCAATGGAGCGAATAATTAATATATAA

Upstream 100 bases:

>100_bases
TCTTTAGTTAAATCATAACCTCCTATATCTTAACATCAAATTCCCGCTTAAGCACATTAATCTATTATTCTAATAAAGAC
TAAACACCTAAATTTTTAAA

Downstream 100 bases:

>100_bases
TAATTAATAAATTTCCTAGTAATTAAAAATTAAAAGTAATGTCAATTATAAAGCTAGAAAAAGGTGACAATAACAACGCA
GCAGTCCTAGAAAAGAAAAC

Product: L,L-diaminopimelate aminotransferase

Products: NA

Alternate protein names: DAP-AT; DAP-aminotransferase; LL-DAP-aminotransferase

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MVQINENYLKLKAGYLFPEISKRVNSYTQANQGSEVIKLGIGDVTEPLPNACINAMSKALNEMGTHEGFKGYGPEQGYEW
LREKISKNDFISRGCQITPEEIFVSDGSKCDSSNILDILGHDNLIAVTDPVYPVYVDSNVMTGRTGETLKNGTYQGLLYL
AINEDNNFLPEIPKNKVDIVYLCFPNNPTGATITKDELKKWVDYANHNKSLILFDAAYEAFIQDKDVPHSIYEIDGAKSC
AIEFRSFSKNAGFTGVRCAYTVIPKCLTGQNSKGDKVDLWPLWNRRQCTKFNGVSYVVQKGAEAVYSSQGKKEVNSLIDF
YMENAKIMRNKLRSAGFTVYGGCNAPYVWIKVPADMTSWDFFDHLLEKANVVGTPGSGFGLAGEGYFRLSAFNSRLNVSN
AMERIINI

Sequences:

>Translated_408_residues
MVQINENYLKLKAGYLFPEISKRVNSYTQANQGSEVIKLGIGDVTEPLPNACINAMSKALNEMGTHEGFKGYGPEQGYEW
LREKISKNDFISRGCQITPEEIFVSDGSKCDSSNILDILGHDNLIAVTDPVYPVYVDSNVMTGRTGETLKNGTYQGLLYL
AINEDNNFLPEIPKNKVDIVYLCFPNNPTGATITKDELKKWVDYANHNKSLILFDAAYEAFIQDKDVPHSIYEIDGAKSC
AIEFRSFSKNAGFTGVRCAYTVIPKCLTGQNSKGDKVDLWPLWNRRQCTKFNGVSYVVQKGAEAVYSSQGKKEVNSLIDF
YMENAKIMRNKLRSAGFTVYGGCNAPYVWIKVPADMTSWDFFDHLLEKANVVGTPGSGFGLAGEGYFRLSAFNSRLNVSN
AMERIINI
>Mature_408_residues
MVQINENYLKLKAGYLFPEISKRVNSYTQANQGSEVIKLGIGDVTEPLPNACINAMSKALNEMGTHEGFKGYGPEQGYEW
LREKISKNDFISRGCQITPEEIFVSDGSKCDSSNILDILGHDNLIAVTDPVYPVYVDSNVMTGRTGETLKNGTYQGLLYL
AINEDNNFLPEIPKNKVDIVYLCFPNNPTGATITKDELKKWVDYANHNKSLILFDAAYEAFIQDKDVPHSIYEIDGAKSC
AIEFRSFSKNAGFTGVRCAYTVIPKCLTGQNSKGDKVDLWPLWNRRQCTKFNGVSYVVQKGAEAVYSSQGKKEVNSLIDF
YMENAKIMRNKLRSAGFTVYGGCNAPYVWIKVPADMTSWDFFDHLLEKANVVGTPGSGFGLAGEGYFRLSAFNSRLNVSN
AMERIINI

Specific function: Involved in the synthesis of meso-diaminopimelate (m-DAP or DL-DAP), required for both lysine and peptidoglycan biosynthesis. Catalyzes the direct conversion of tetrahydrodipicolinate to LL-diaminopimelate, a reaction that requires three enzymes in E.coli

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. LL-diaminopimelate aminotransferase subfamily

Homologues:

Organism=Escherichia coli, GI1788722, Length=384, Percent_Identity=25.5208333333333, Blast_Score=108, Evalue=8e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPAT_PROM5 (A2BYM6)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011994.1
- ProteinModelPortal:   A2BYM6
- SMR:   A2BYM6
- STRING:   A2BYM6
- GeneID:   4720189
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_16801
- eggNOG:   COG0436
- HOGENOM:   HBG645860
- OMA:   VDLIYLC
- ProtClustDB:   PRK07590
- BioCyc:   PMAR167542:P9515ORF_1752-MONOMER
- HAMAP:   MF_01642
- InterPro:   IPR004839
- InterPro:   IPR019942
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR03542

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.83

Molecular weight: Translated: 45477; Mature: 45477

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: BINDING 42-42 BINDING 72-72 BINDING 75-75 BINDING 109-109 BINDING 132-132 BINDING 187-187 BINDING 187-187 BINDING 215-215 BINDING 218-218 BINDING 246-246 BINDING 248-248 BINDING 257-257 BINDING 292-292 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQINENYLKLKAGYLFPEISKRVNSYTQANQGSEVIKLGIGDVTEPLPNACINAMSKAL
CEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
NEMGTHEGFKGYGPEQGYEWLREKISKNDFISRGCQITPEEIFVSDGSKCDSSNILDILG
HHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHEEECCCCCCCCCCEEEEEC
HDNLIAVTDPVYPVYVDSNVMTGRTGETLKNGTYQGLLYLAINEDNNFLPEIPKNKVDIV
CCCEEEEECCCEEEEECCCEECCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCEE
YLCFPNNPTGATITKDELKKWVDYANHNKSLILFDAAYEAFIQDKDVPHSIYEIDGAKSC
EEEECCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCHHEEECCCCHH
AIEFRSFSKNAGFTGVRCAYTVIPKCLTGQNSKGDKVDLWPLWNRRQCTKFNGVSYVVQK
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEECCHHHHHHH
GAEAVYSSQGKKEVNSLIDFYMENAKIMRNKLRSAGFTVYGGCNAPYVWIKVPADMTSWD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCHHH
FFDHLLEKANVVGTPGSGFGLAGEGYFRLSAFNSRLNVSNAMERIINI
HHHHHHHHCCEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MVQINENYLKLKAGYLFPEISKRVNSYTQANQGSEVIKLGIGDVTEPLPNACINAMSKAL
CEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
NEMGTHEGFKGYGPEQGYEWLREKISKNDFISRGCQITPEEIFVSDGSKCDSSNILDILG
HHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHEEECCCCCCCCCCEEEEEC
HDNLIAVTDPVYPVYVDSNVMTGRTGETLKNGTYQGLLYLAINEDNNFLPEIPKNKVDIV
CCCEEEEECCCEEEEECCCEECCCCCCHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCEE
YLCFPNNPTGATITKDELKKWVDYANHNKSLILFDAAYEAFIQDKDVPHSIYEIDGAKSC
EEEECCCCCCCCEEHHHHHHHHHHCCCCCEEEEEEHHHHHHHCCCCCCCHHEEECCCCHH
AIEFRSFSKNAGFTGVRCAYTVIPKCLTGQNSKGDKVDLWPLWNRRQCTKFNGVSYVVQK
HHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEECCHHHHHHH
GAEAVYSSQGKKEVNSLIDFYMENAKIMRNKLRSAGFTVYGGCNAPYVWIKVPADMTSWD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEEECCCCCCHHH
FFDHLLEKANVVGTPGSGFGLAGEGYFRLSAFNSRLNVSNAMERIINI
HHHHHHHHCCEECCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA