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The map label for this gene is miaB

Identifier: 123966699

GI number: 123966699

Start: 1303613

End: 1305007

Strand: Direct

Name: miaB

Synonym: P9515_14661

Alternate gene names: 123966699

Gene position: 1303613-1305007 (Clockwise)

Preceding gene: 123966696

Following gene: 123966700

Centisome position: 76.5

GC content: 32.97

Gene sequence:

>1395_bases
GTGTTAACAAAAACAATTCAAGAAGAAAAAAAAACTCCCAAAGATGAGTCTATTGGTAGTTATTGGATTACAACATTCGG
TTGTCAAATGAATAAGGCTGATTCTGAAAGGATGGCTGGCACCTTAGAAAAAATGGGATATTCAAGAGCTATTGATGAAT
TAAAAGCTGATTTGGTCTTGTATAACACTTGTACAATAAGAGATAGCGCTCAACAAAAAGTTTATAGTTTTTTAGGAAGG
CAAATCAAAAGAAAGCATAGTTTACCAAAGTTAAAATTAGTTGTCGCCGGTTGCCTAGCTCAACAAGAGGGGGAGTCTCT
TTTAAGAAGAGTTCCAGAACTAGATTTAATAATGGGGCCTCAACATGTTAATAATCTTGAAAATCTTTTAGAGAGAGTTG
ATTCAGGTAATCAAGTCGTTGCCACCGAAGAAACTTTTATATCTGAAGACATCACTAATGCAAGAAGAGATAGCACAATT
TGTGGTTGGGTGAATATCATTTATGGTTGCAATGAAAGATGTTCTTATTGTGTCGTCCCCTCTGTTAGAGGGAAAGAACA
GTCAAGATATCCAAAGGCAATTAAAAGTGAAATACAAACTTTAGCCCAAAATAATTATAAGGAAATTACTCTTTTAGGTC
AGAATATTGATGCCTATGGAAGAGATCTTCCTGTCACAACAAAAGAAGGGAGGAAAGAAAATACCCTTACTGATCTCCTT
TATTTTATTCATGATGTAGATGGAATTAGAAGAATAAGATTTTCTACTAGTCATCCAAAATATTTTTCAAAAAGACTTAT
AAAAGCTTGTTATGAATTAGATAAAGTTTGTGAGCATTTTCATATTCCTTTCCAAAGTGGAAATGATGAAATCCTGAGAT
TAATGGCAAGAGGTTACACAATCGATAAATATAAAAAAATAATTGAAAATATAAGATCATTAATGCCAAATGCATCAATT
ACTGCAGATGCAATAGTTGCTTTCCCCGGTGAAACAGAAAAACAATATCAAGATACGTTAAGGTTGATATCCGACGTTGG
TTTTGATCAAGTGATGACCGCGGCATATTCTCCTAGACCAAATACTCCAGCAGCGATCTGGAATAATCAAATTGCTGAAG
AAGTTAAAAAAGAAAGATTAAAAGAAATTAACGAATTAGTTGAAGCAACCTCAAGGAAAAGAAATCAAAGATATCTAAAT
AATACTGAAAGTGTTTTGATAGAAGGTTTGAATCCAAAAAACTCCATGCAAATGATGGGTAGGACAAGAACTAACAGACT
TACTTTTGTAGAAATCCCCGAAAACATTGAATTTAATTATTCTTTTGGAGATGAACTAAATGTCAAAATAACGGAAGCGA
GATCTTTTTCTTTAAGCGGTCAAATTTATAAGTAA

Upstream 100 bases:

>100_bases
TCGGGGGTTCGAATCCCCTCGCTCGCCCTCAAAAATATGACCCCAAGATTATTTAACTTATAATTTTGAATTAACGAATC
CCCACAAAACTTTTTGCAAC

Downstream 100 bases:

>100_bases
TCTTTCCAAATGGTAAGAAACGAAAAAAAATGTATCGGAATAATATTTGGGGGCACTTCAAATGAACATTATGTATCAAT
ATCTTCCGCCAAAACAGTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLTKTIQEEKKTPKDESIGSYWITTFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVLYNTCTIRDSAQQKVYSFLGR
QIKRKHSLPKLKLVVAGCLAQQEGESLLRRVPELDLIMGPQHVNNLENLLERVDSGNQVVATEETFISEDITNARRDSTI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPKAIKSEIQTLAQNNYKEITLLGQNIDAYGRDLPVTTKEGRKENTLTDLL
YFIHDVDGIRRIRFSTSHPKYFSKRLIKACYELDKVCEHFHIPFQSGNDEILRLMARGYTIDKYKKIIENIRSLMPNASI
TADAIVAFPGETEKQYQDTLRLISDVGFDQVMTAAYSPRPNTPAAIWNNQIAEEVKKERLKEINELVEATSRKRNQRYLN
NTESVLIEGLNPKNSMQMMGRTRTNRLTFVEIPENIEFNYSFGDELNVKITEARSFSLSGQIYK

Sequences:

>Translated_464_residues
MLTKTIQEEKKTPKDESIGSYWITTFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVLYNTCTIRDSAQQKVYSFLGR
QIKRKHSLPKLKLVVAGCLAQQEGESLLRRVPELDLIMGPQHVNNLENLLERVDSGNQVVATEETFISEDITNARRDSTI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPKAIKSEIQTLAQNNYKEITLLGQNIDAYGRDLPVTTKEGRKENTLTDLL
YFIHDVDGIRRIRFSTSHPKYFSKRLIKACYELDKVCEHFHIPFQSGNDEILRLMARGYTIDKYKKIIENIRSLMPNASI
TADAIVAFPGETEKQYQDTLRLISDVGFDQVMTAAYSPRPNTPAAIWNNQIAEEVKKERLKEINELVEATSRKRNQRYLN
NTESVLIEGLNPKNSMQMMGRTRTNRLTFVEIPENIEFNYSFGDELNVKITEARSFSLSGQIYK
>Mature_464_residues
MLTKTIQEEKKTPKDESIGSYWITTFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVLYNTCTIRDSAQQKVYSFLGR
QIKRKHSLPKLKLVVAGCLAQQEGESLLRRVPELDLIMGPQHVNNLENLLERVDSGNQVVATEETFISEDITNARRDSTI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPKAIKSEIQTLAQNNYKEITLLGQNIDAYGRDLPVTTKEGRKENTLTDLL
YFIHDVDGIRRIRFSTSHPKYFSKRLIKACYELDKVCEHFHIPFQSGNDEILRLMARGYTIDKYKKIIENIRSLMPNASI
TADAIVAFPGETEKQYQDTLRLISDVGFDQVMTAAYSPRPNTPAAIWNNQIAEEVKKERLKEINELVEATSRKRNQRYLN
NTESVLIEGLNPKNSMQMMGRTRTNRLTFVEIPENIEFNYSFGDELNVKITEARSFSLSGQIYK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=485, Percent_Identity=32.3711340206186, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI28872782, Length=485, Percent_Identity=32.3711340206186, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI93277076, Length=406, Percent_Identity=28.0788177339901, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1786882, Length=448, Percent_Identity=37.9464285714286, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=28.159645232816, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=32.6881720430108, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=372, Percent_Identity=25, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356207, Length=470, Percent_Identity=31.063829787234, Blast_Score=229, Evalue=4e-60,
Organism=Drosophila melanogaster, GI19922432, Length=482, Percent_Identity=25.3112033195021, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PROM5 (A2BY12)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011780.1
- ProteinModelPortal:   A2BY12
- SMR:   A2BY12
- STRING:   A2BY12
- GeneID:   4720113
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_14661
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14333
- BioCyc:   PMAR167542:P9515ORF_1532-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53153; Mature: 53153

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM; PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKTIQEEKKTPKDESIGSYWITTFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVL
CCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEE
YNTCTIRDSAQQKVYSFLGRQIKRKHSLPKLKLVVAGCLAQQEGESLLRRVPELDLIMGP
EEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLERVDSGNQVVATEETFISEDITNARRDSTICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPKAIKSEIQTLAQNNYKEITLLGQNIDAYGRDLPVTTKEGRKENTLTDLL
CCCCCCHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
YFIHDVDGIRRIRFSTSHPKYFSKRLIKACYELDKVCEHFHIPFQSGNDEILRLMARGYT
HHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IDKYKKIIENIRSLMPNASITADAIVAFPGETEKQYQDTLRLISDVGFDQVMTAAYSPRP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
NTPAAIWNNQIAEEVKKERLKEINELVEATSRKRNQRYLNNTESVLIEGLNPKNSMQMMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
RTRTNRLTFVEIPENIEFNYSFGDELNVKITEARSFSLSGQIYK
CCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCEECCCCCCC
>Mature Secondary Structure
MLTKTIQEEKKTPKDESIGSYWITTFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVL
CCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCEEE
YNTCTIRDSAQQKVYSFLGRQIKRKHSLPKLKLVVAGCLAQQEGESLLRRVPELDLIMGP
EEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLERVDSGNQVVATEETFISEDITNARRDSTICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPKAIKSEIQTLAQNNYKEITLLGQNIDAYGRDLPVTTKEGRKENTLTDLL
CCCCCCHHCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH
YFIHDVDGIRRIRFSTSHPKYFSKRLIKACYELDKVCEHFHIPFQSGNDEILRLMARGYT
HHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IDKYKKIIENIRSLMPNASITADAIVAFPGETEKQYQDTLRLISDVGFDQVMTAAYSPRP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCC
NTPAAIWNNQIAEEVKKERLKEINELVEATSRKRNQRYLNNTESVLIEGLNPKNSMQMMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHH
RTRTNRLTFVEIPENIEFNYSFGDELNVKITEARSFSLSGQIYK
CCCCCCEEEEECCCCEEEEEECCCCEEEEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA