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The map label for this gene is ddl

Identifier: 123966700

GI number: 123966700

Start: 1305017

End: 1306084

Strand: Direct

Name: ddl

Synonym: P9515_14671

Alternate gene names: 123966700

Gene position: 1305017-1306084 (Clockwise)

Preceding gene: 123966699

Following gene: 123966701

Centisome position: 76.58

GC content: 29.31

Gene sequence:

>1068_bases
ATGGTAAGAAACGAAAAAAAATGTATCGGAATAATATTTGGGGGCACTTCAAATGAACATTATGTATCAATATCTTCCGC
CAAAACAGTTTTTAAAGCTCTAATTTCAAGAACAAATAAAGAACTATTCAGGATTAAAGCTTTTTACATAAATAAACATG
GAGTTTGGATTGATAGCGATTTATCTCTAGAAATACTTAGAGAAAATAGTGGAAATAATATCTTCGAAAAATATCAAGAA
TTGCCTAACAGAAAAATAAACTTTCTCAACAATCTTGAATTCCAAAATATTGATATTTGGTTTCCTTTATTACATGGTTG
CAACGGAGAAGATGGGGCTATTCATGGATTACTAAAATTCACCCAAAAACCTTTGATTGGATGTGGAATTTCAGGTTCTG
CTATTGGAATGGATAAAATATTGATGAAACAAATATTTTCAAATTTGTCAATTCCACAAGTGAATTTTTTAGCCATCCAA
AATTATGATCTAAGTGATGATAATGTTAAAGATAATTTATCTGTTGAAATAATTGAGAAATTAAATTTACCAGTTTTTGT
AAAACCTGCGAATTCAGGATCATCTCTTGGCATTTCTAAAGCTAAAAATAAATCAGAAATAATTAAAGCTTTACAAAAGG
CTTGGGAAATAGATTCAAGAATTGTTATCGAAGAGGGATTGAATGTTAGAGAACTTGAATGTGGAATAATTGGCAATCTA
AAACTTTCTACATCAAAAATTGGGGAAGTTTCCTATTCATCAGATTGGTACGATTATGATTCAAAATACTCTACGGATAA
TAAAATAATTATTCCAGCTGATATTGATTCTCAAATCTCTGAACAAATTAAAGATCTAGCTATTCGAAGTTGTAGAGCAT
TAAATATTTATGGTTTTGCAAGGGTAGATTTCTTTTTAGAAAAAATTTCAAGGAAAATCTTCTTAAATGAAATAAATACC
ATCCCTGGCTTTACTAGCAAAAGTATGTTTCCAATGCTTTGGAAGGCCTCAGGTTTAAATATTGATCAACTTGTAGCTAA
ACTGATTGATATATCCTTAGATTCATAG

Upstream 100 bases:

>100_bases
CATTGAATTTAATTATTCTTTTGGAGATGAACTAAATGTCAAAATAACGGAAGCGAGATCTTTTTCTTTAAGCGGTCAAA
TTTATAAGTAATCTTTCCAA

Downstream 100 bases:

>100_bases
AATAAAGTCAAATGCTGCATGGACTACTTTGGTTACCATTACTTTTAATCTTCGTTCTATTAACTGCTCTTGGTTGGCTG
GAAAGAAGAAGACAAAATCT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MVRNEKKCIGIIFGGTSNEHYVSISSAKTVFKALISRTNKELFRIKAFYINKHGVWIDSDLSLEILRENSGNNIFEKYQE
LPNRKINFLNNLEFQNIDIWFPLLHGCNGEDGAIHGLLKFTQKPLIGCGISGSAIGMDKILMKQIFSNLSIPQVNFLAIQ
NYDLSDDNVKDNLSVEIIEKLNLPVFVKPANSGSSLGISKAKNKSEIIKALQKAWEIDSRIVIEEGLNVRELECGIIGNL
KLSTSKIGEVSYSSDWYDYDSKYSTDNKIIIPADIDSQISEQIKDLAIRSCRALNIYGFARVDFFLEKISRKIFLNEINT
IPGFTSKSMFPMLWKASGLNIDQLVAKLIDISLDS

Sequences:

>Translated_355_residues
MVRNEKKCIGIIFGGTSNEHYVSISSAKTVFKALISRTNKELFRIKAFYINKHGVWIDSDLSLEILRENSGNNIFEKYQE
LPNRKINFLNNLEFQNIDIWFPLLHGCNGEDGAIHGLLKFTQKPLIGCGISGSAIGMDKILMKQIFSNLSIPQVNFLAIQ
NYDLSDDNVKDNLSVEIIEKLNLPVFVKPANSGSSLGISKAKNKSEIIKALQKAWEIDSRIVIEEGLNVRELECGIIGNL
KLSTSKIGEVSYSSDWYDYDSKYSTDNKIIIPADIDSQISEQIKDLAIRSCRALNIYGFARVDFFLEKISRKIFLNEINT
IPGFTSKSMFPMLWKASGLNIDQLVAKLIDISLDS
>Mature_355_residues
MVRNEKKCIGIIFGGTSNEHYVSISSAKTVFKALISRTNKELFRIKAFYINKHGVWIDSDLSLEILRENSGNNIFEKYQE
LPNRKINFLNNLEFQNIDIWFPLLHGCNGEDGAIHGLLKFTQKPLIGCGISGSAIGMDKILMKQIFSNLSIPQVNFLAIQ
NYDLSDDNVKDNLSVEIIEKLNLPVFVKPANSGSSLGISKAKNKSEIIKALQKAWEIDSRIVIEEGLNVRELECGIIGNL
KLSTSKIGEVSYSSDWYDYDSKYSTDNKIIIPADIDSQISEQIKDLAIRSCRALNIYGFARVDFFLEKISRKIFLNEINT
IPGFTSKSMFPMLWKASGLNIDQLVAKLIDISLDS

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=362, Percent_Identity=35.3591160220994, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786280, Length=349, Percent_Identity=28.080229226361, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PROM5 (A2BY13)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011781.1
- ProteinModelPortal:   A2BY13
- SMR:   A2BY13
- STRING:   A2BY13
- GeneID:   4720114
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_14671
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   PMAR167542:P9515ORF_1533-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 39961; Mature: 39961

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRNEKKCIGIIFGGTSNEHYVSISSAKTVFKALISRTNKELFRIKAFYINKHGVWIDSD
CCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCHHEEEEEEEEEECCCEEECCC
LSLEILRENSGNNIFEKYQELPNRKINFLNNLEFQNIDIWFPLLHGCNGEDGAIHGLLKF
CEEEEEECCCCCHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEHHHCCCCCCCHHHHHHHH
TQKPLIGCGISGSAIGMDKILMKQIFSNLSIPQVNFLAIQNYDLSDDNVKDNLSVEIIEK
HCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEHHH
LNLPVFVKPANSGSSLGISKAKNKSEIIKALQKAWEIDSRIVIEEGLNVRELECGIIGNL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCEEECE
KLSTSKIGEVSYSSDWYDYDSKYSTDNKIIIPADIDSQISEQIKDLAIRSCRALNIYGFA
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEHH
RVDFFLEKISRKIFLNEINTIPGFTSKSMFPMLWKASGLNIDQLVAKLIDISLDS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRNEKKCIGIIFGGTSNEHYVSISSAKTVFKALISRTNKELFRIKAFYINKHGVWIDSD
CCCCCCEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHCCHHEEEEEEEEEECCCEEECCC
LSLEILRENSGNNIFEKYQELPNRKINFLNNLEFQNIDIWFPLLHGCNGEDGAIHGLLKF
CEEEEEECCCCCHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEHHHCCCCCCCHHHHHHHH
TQKPLIGCGISGSAIGMDKILMKQIFSNLSIPQVNFLAIQNYDLSDDNVKDNLSVEIIEK
HCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCEEEHHH
LNLPVFVKPANSGSSLGISKAKNKSEIIKALQKAWEIDSRIVIEEGLNVRELECGIIGNL
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCEEECE
KLSTSKIGEVSYSSDWYDYDSKYSTDNKIIIPADIDSQISEQIKDLAIRSCRALNIYGFA
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEHH
RVDFFLEKISRKIFLNEINTIPGFTSKSMFPMLWKASGLNIDQLVAKLIDISLDS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA