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The map label for this gene is folC [H]

Identifier: 123966696

GI number: 123966696

Start: 1299472

End: 1300704

Strand: Direct

Name: folC [H]

Synonym: P9515_14631

Alternate gene names: 123966696

Gene position: 1299472-1300704 (Clockwise)

Preceding gene: 123966695

Following gene: 123966699

Centisome position: 76.25

GC content: 29.93

Gene sequence:

>1233_bases
TTGAAAAAAGAAAACTTTAAGAATTTTGATTTGCTTTCACCTAAATTTGAGAGACAAAATATTCACTTAGGATTATCAAG
AATTAAAAAAGCGCTTAAGAAATTAAATGATCCTTGCAAAAACATCCCAGCTATTCAAGTTGTTGGAACAAATGGGAAAG
GTTCGATTACTGCTTTTATAGAAAATATTCTTTATGAAGAGAAAAAGAATATTGGGGTTACCACTTCGCCTCACCTTTTA
GATATATGTGAAAGGATCAGAGTGAATAAAGAAAATATTCATAAAGAAGAATTTGAGAGCTTATTTAAGAAAATTGCAAC
TTATCTTTCAATCTATAAATTATCCCCTTTTGAACAGATTATTTGCTGTGCACTAGTTTTTTTTGATATGAAAAAAGTCG
ATTTATTGATACTTGAAGCTGGTTTAGGTGGAAGATTAGATGCAACTACAGCTCATAATCTAAGACCAATAATTGCCATT
GGAAATATAGATTTAGACCACAAAGAGTATCTTGGAGATTCGATAGAAAAAATTACCAAGGAAAAAGTTGCTGTAATTGA
AAAAAATGCATTTGTTATTTCTTTTCGTCAAAAAACTAAAGTTGAGGAAATAATTAAAGCAAGAGCCAAAAAAGTAGGTG
CACAAATCATATGGAAGGAATCACTATCTAATGAAATTGAACTTGGTTTAAATGGAGCTTTTCAAAGACAAAATGCCGCA
GTTGCTATTGGAGTAATTGAGGCACTAAATAATTTAGGGTTCAATGTCAGAGAATGTTCCATTAAGGAAGGACTTAAGAA
CACAAAATGGAATGGGAGGTTAGAAATAATTAATTGTTTAAATAAAAAGATACTTGTCGATTCTGCACATAATTACTCTG
CGGCAATGGCTCTTTCAGAGGAAAGAAAAACTTGGAATAATCAAGAGGAAGGTGTCTATTGGATTTTAGGGGTCCAAAAA
AGTAAAGACATTGTATCTATGCTAAAAGTTCTTATTAAAAAAAATGATCACATATTATTAGTTCCTGTTCCTAATCAAAC
TTGTTGGAGATTAGAGGATCTTTTAGATAATGCTGAACTTGAAAATTTAAATATAATAGATTTTGAAAAAATTGATTATG
CTTTTAATTATCTTTTGGAACTTAGAACATGGCCAAAATGTCATCCCGTACTAACTGGCTCAATTTTTTTAGTCTCTGAG
TTTATTAAATTCGCAAATAATCAAAATTATTAA

Upstream 100 bases:

>100_bases
GGTTAGGTTTGTTCCACCATTGATAATTTCGCGAAATGAAATCAAAATACTTCTTAAGAAGTTGAATTTAATTTTTGAAG
AACTGTAAGCTTTAGAGGAT

Downstream 100 bases:

>100_bases
AGATTAAAGTCATCTTTTATTTCCCATCCTTCCAATTTCCCAGGATTTAACAACCCTTTAGGATCGAATTTTAATTTTGC
TTTTACTTGATCAGAATCAA

Product: putative bifunctional dihydrofolate/folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKKENFKNFDLLSPKFERQNIHLGLSRIKKALKKLNDPCKNIPAIQVVGTNGKGSITAFIENILYEEKKNIGVTTSPHLL
DICERIRVNKENIHKEEFESLFKKIATYLSIYKLSPFEQIICCALVFFDMKKVDLLILEAGLGGRLDATTAHNLRPIIAI
GNIDLDHKEYLGDSIEKITKEKVAVIEKNAFVISFRQKTKVEEIIKARAKKVGAQIIWKESLSNEIELGLNGAFQRQNAA
VAIGVIEALNNLGFNVRECSIKEGLKNTKWNGRLEIINCLNKKILVDSAHNYSAAMALSEERKTWNNQEEGVYWILGVQK
SKDIVSMLKVLIKKNDHILLVPVPNQTCWRLEDLLDNAELENLNIIDFEKIDYAFNYLLELRTWPKCHPVLTGSIFLVSE
FIKFANNQNY

Sequences:

>Translated_410_residues
MKKENFKNFDLLSPKFERQNIHLGLSRIKKALKKLNDPCKNIPAIQVVGTNGKGSITAFIENILYEEKKNIGVTTSPHLL
DICERIRVNKENIHKEEFESLFKKIATYLSIYKLSPFEQIICCALVFFDMKKVDLLILEAGLGGRLDATTAHNLRPIIAI
GNIDLDHKEYLGDSIEKITKEKVAVIEKNAFVISFRQKTKVEEIIKARAKKVGAQIIWKESLSNEIELGLNGAFQRQNAA
VAIGVIEALNNLGFNVRECSIKEGLKNTKWNGRLEIINCLNKKILVDSAHNYSAAMALSEERKTWNNQEEGVYWILGVQK
SKDIVSMLKVLIKKNDHILLVPVPNQTCWRLEDLLDNAELENLNIIDFEKIDYAFNYLLELRTWPKCHPVLTGSIFLVSE
FIKFANNQNY
>Mature_410_residues
MKKENFKNFDLLSPKFERQNIHLGLSRIKKALKKLNDPCKNIPAIQVVGTNGKGSITAFIENILYEEKKNIGVTTSPHLL
DICERIRVNKENIHKEEFESLFKKIATYLSIYKLSPFEQIICCALVFFDMKKVDLLILEAGLGGRLDATTAHNLRPIIAI
GNIDLDHKEYLGDSIEKITKEKVAVIEKNAFVISFRQKTKVEEIIKARAKKVGAQIIWKESLSNEIELGLNGAFQRQNAA
VAIGVIEALNNLGFNVRECSIKEGLKNTKWNGRLEIINCLNKKILVDSAHNYSAAMALSEERKTWNNQEEGVYWILGVQK
SKDIVSMLKVLIKKNDHILLVPVPNQTCWRLEDLLDNAELENLNIIDFEKIDYAFNYLLELRTWPKCHPVLTGSIFLVSE
FIKFANNQNY

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=289, Percent_Identity=28.0276816608997, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI66932984, Length=289, Percent_Identity=28.0276816608997, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1788654, Length=358, Percent_Identity=26.8156424581006, Blast_Score=116, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17553148, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71984923, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17553150, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=430, Percent_Identity=26.7441860465116, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6324815, Length=293, Percent_Identity=30.3754266211604, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322718, Length=428, Percent_Identity=24.2990654205607, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581568, Length=194, Percent_Identity=31.9587628865979, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24641571, Length=274, Percent_Identity=27.3722627737226, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 46785; Mature: 46785

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKENFKNFDLLSPKFERQNIHLGLSRIKKALKKLNDPCKNIPAIQVVGTNGKGSITAFI
CCCCCCCCCCCCCCCCCCCHHEECHHHHHHHHHHHCCHHHCCCEEEEEECCCCCHHHHHH
ENILYEEKKNIGVTTSPHLLDICERIRVNKENIHKEEFESLFKKIATYLSIYKLSPFEQI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH
ICCALVFFDMKKVDLLILEAGLGGRLDATTAHNLRPIIAIGNIDLDHKEYLGDSIEKITK
HHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
EKVAVIEKNAFVISFRQKTKVEEIIKARAKKVGAQIIWKESLSNEIELGLNGAFQRQNAA
HHHHEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCEEECCCCCCCCCCCE
VAIGVIEALNNLGFNVRECSIKEGLKNTKWNGRLEIINCLNKKILVDSAHNYSAAMALSE
EEHHHHHHHHHCCCCEEECHHHHCCCCCCCCCEEHHHHHCCCEEEEECCCCCHHHHHHHH
ERKTWNNQEEGVYWILGVQKSKDIVSMLKVLIKKNDHILLVPVPNQTCWRLEDLLDNAEL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCC
ENLNIIDFEKIDYAFNYLLELRTWPKCHPVLTGSIFLVSEFIKFANNQNY
CCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKENFKNFDLLSPKFERQNIHLGLSRIKKALKKLNDPCKNIPAIQVVGTNGKGSITAFI
CCCCCCCCCCCCCCCCCCCHHEECHHHHHHHHHHHCCHHHCCCEEEEEECCCCCHHHHHH
ENILYEEKKNIGVTTSPHLLDICERIRVNKENIHKEEFESLFKKIATYLSIYKLSPFEQI
HHHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHH
ICCALVFFDMKKVDLLILEAGLGGRLDATTAHNLRPIIAIGNIDLDHKEYLGDSIEKITK
HHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
EKVAVIEKNAFVISFRQKTKVEEIIKARAKKVGAQIIWKESLSNEIELGLNGAFQRQNAA
HHHHEEECCEEEEEEHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCEEECCCCCCCCCCCE
VAIGVIEALNNLGFNVRECSIKEGLKNTKWNGRLEIINCLNKKILVDSAHNYSAAMALSE
EEHHHHHHHHHCCCCEEECHHHHCCCCCCCCCEEHHHHHCCCEEEEECCCCCHHHHHHHH
ERKTWNNQEEGVYWILGVQKSKDIVSMLKVLIKKNDHILLVPVPNQTCWRLEDLLDNAEL
HHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCC
ENLNIIDFEKIDYAFNYLLELRTWPKCHPVLTGSIFLVSEFIKFANNQNY
CCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]