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The map label for this gene is bioB

Identifier: 123966417

GI number: 123966417

Start: 1040813

End: 1041820

Strand: Direct

Name: bioB

Synonym: P9515_11841

Alternate gene names: 123966417

Gene position: 1040813-1041820 (Clockwise)

Preceding gene: 123966416

Following gene: 123966418

Centisome position: 61.07

GC content: 34.92

Gene sequence:

>1008_bases
ATGATTAATTCAGATAATCAAAAATTTAGCAAAATTAGATTTGACTGGGCAAGGGATGAGATTTTGGAAATATTAAATTA
CCCATTAGTAGATTTAATGTGGGAAGCTCAAATTATTCATAGAAGATTTAATGAATATAAAGTTCAACTAGCATCTCTTT
TCAGTGTGAAAACAGGCGGATGTGAAGAAAATTGTTCTTACTGTAGCCAATCAATTTATAGCTCTAGTCAAATAAAGAGT
CACCCTCAATTTGAAGTTGAAGCTGTTTTAAACAGAGCCAAAACAGCAAAAAAAGAAGGGGCTGATAGGTTTTGTATGGG
TTGGGCTTGGAGAGAAATTAGAGATGGCAAACCTTTCAACTCAATGCTTGAAATGGTTAAAGGTGTAAAAGAACTTGGAA
TGGAGGCATGTGTAACTGCAGGGATGCTTACAGATGAGCAGGCCTTGAGACTTGCTGATGCGGGACTAACTGCATACAAC
CATAATCTTGATACGAGTCCCGAATACTATAAAAATATTATTACGACAAGAACTTATCAAGACAGATTAGAAACTATTAA
AAGAGTCAGAAATGCAGGTATAAATGTATGTTGCGGTGGAATAATAGGTCTTGGAGAAAATAATGGAGATAGAGCATCTC
TTTTAGAAGTTCTATCAAACATGAATCCTCACCCTGAAAGTGTCCCAATCAATTCTTTGGTAGCTATAGAAGGAACAGGT
TTGGAGGAAAAAAAAGAAATTGATTCCATTGAGATGATAAGAATGATTGCTACTGCTCGGATACTTATGCCAAAAAGTAA
AATAAGATTAAGTGCGGGAAGGGAGAAGTTAACAAAAGAAGCTCAGATTATTTGTTTTCAATGTGGAGCAAACTCTATTT
TTTATGGAGACGAATTACTGACCACATCGAATCCATCTTTTCAAGATGACCGGAAACTACTTAAGGATGTAGGAGTCTTA
TTTAATAAAGATTTTGAATATTGTGATAAAACTGTCTCGACAGTATGA

Upstream 100 bases:

>100_bases
AGCAATTATTGACTATCAATCAAGGGATAGGCGTTTTGGAGGTATACAATCATTACCAAATAAATCTTGCAAAGATTCTA
ACTATTCTTCCTAATCAAAA

Downstream 100 bases:

>100_bases
AGAAGAATAATTATAAAATAGTTTCACTTTATTCTTTTTTCTCATTTCAAGAAAATTCAATAATTGAACTTAAGCAAAAC
TTGTTAAGGATTGAGAAAGA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MINSDNQKFSKIRFDWARDEILEILNYPLVDLMWEAQIIHRRFNEYKVQLASLFSVKTGGCEENCSYCSQSIYSSSQIKS
HPQFEVEAVLNRAKTAKKEGADRFCMGWAWREIRDGKPFNSMLEMVKGVKELGMEACVTAGMLTDEQALRLADAGLTAYN
HNLDTSPEYYKNIITTRTYQDRLETIKRVRNAGINVCCGGIIGLGENNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEEKKEIDSIEMIRMIATARILMPKSKIRLSAGREKLTKEAQIICFQCGANSIFYGDELLTTSNPSFQDDRKLLKDVGVL
FNKDFEYCDKTVSTV

Sequences:

>Translated_335_residues
MINSDNQKFSKIRFDWARDEILEILNYPLVDLMWEAQIIHRRFNEYKVQLASLFSVKTGGCEENCSYCSQSIYSSSQIKS
HPQFEVEAVLNRAKTAKKEGADRFCMGWAWREIRDGKPFNSMLEMVKGVKELGMEACVTAGMLTDEQALRLADAGLTAYN
HNLDTSPEYYKNIITTRTYQDRLETIKRVRNAGINVCCGGIIGLGENNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEEKKEIDSIEMIRMIATARILMPKSKIRLSAGREKLTKEAQIICFQCGANSIFYGDELLTTSNPSFQDDRKLLKDVGVL
FNKDFEYCDKTVSTV
>Mature_335_residues
MINSDNQKFSKIRFDWARDEILEILNYPLVDLMWEAQIIHRRFNEYKVQLASLFSVKTGGCEENCSYCSQSIYSSSQIKS
HPQFEVEAVLNRAKTAKKEGADRFCMGWAWREIRDGKPFNSMLEMVKGVKELGMEACVTAGMLTDEQALRLADAGLTAYN
HNLDTSPEYYKNIITTRTYQDRLETIKRVRNAGINVCCGGIIGLGENNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
LEEKKEIDSIEMIRMIATARILMPKSKIRLSAGREKLTKEAQIICFQCGANSIFYGDELLTTSNPSFQDDRKLLKDVGVL
FNKDFEYCDKTVSTV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=308, Percent_Identity=50.974025974026, Blast_Score=332, Evalue=3e-92,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=45.9375, Blast_Score=305, Evalue=7e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_PROM5 (A2BX80)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011498.1
- ProteinModelPortal:   A2BX80
- SMR:   A2BX80
- STRING:   A2BX80
- GeneID:   4718947
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_11841
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2391426
- BioCyc:   PMAR167542:P9515ORF_1238-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37832; Mature: 37832

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSDNQKFSKIRFDWARDEILEILNYPLVDLMWEAQIIHRRFNEYKVQLASLFSVKTGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
CEENCSYCSQSIYSSSQIKSHPQFEVEAVLNRAKTAKKEGADRFCMGWAWREIRDGKPFN
CCHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
SMLEMVKGVKELGMEACVTAGMLTDEQALRLADAGLTAYNHNLDTSPEYYKNIITTRTYQ
HHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCHHH
DRLETIKRVRNAGINVCCGGIIGLGENNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
HHHHHHHHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
LEEKKEIDSIEMIRMIATARILMPKSKIRLSAGREKLTKEAQIICFQCGANSIFYGDELL
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCEEE
TTSNPSFQDDRKLLKDVGVLFNKDFEYCDKTVSTV
ECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure
MINSDNQKFSKIRFDWARDEILEILNYPLVDLMWEAQIIHRRFNEYKVQLASLFSVKTGG
CCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
CEENCSYCSQSIYSSSQIKSHPQFEVEAVLNRAKTAKKEGADRFCMGWAWREIRDGKPFN
CCHHHHHHHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHH
SMLEMVKGVKELGMEACVTAGMLTDEQALRLADAGLTAYNHNLDTSPEYYKNIITTRTYQ
HHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHCCHHH
DRLETIKRVRNAGINVCCGGIIGLGENNGDRASLLEVLSNMNPHPESVPINSLVAIEGTG
HHHHHHHHHHHCCCEEEECCEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
LEEKKEIDSIEMIRMIATARILMPKSKIRLSAGREKLTKEAQIICFQCGANSIFYGDELL
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEEEECCCCEEECCEEE
TTSNPSFQDDRKLLKDVGVLFNKDFEYCDKTVSTV
ECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA