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The map label for this gene is ksgA [H]

Identifier: 123966249

GI number: 123966249

Start: 909296

End: 910126

Strand: Reverse

Name: ksgA [H]

Synonym: P9515_10161

Alternate gene names: 123966249

Gene position: 910126-909296 (Counterclockwise)

Preceding gene: 123966255

Following gene: 123966248

Centisome position: 53.41

GC content: 28.28

Gene sequence:

>831_bases
ATGAATTTTAAGAACCATCATCAAAAAAAAAGATTTGGACAACATTGGTTGGTTAATAATTTAATATTGGAAAAAATAAA
AGAAGTCGCTGAACTCGATGAAAAAGATTTTATTCTAGAAATTGGTCCGGGAAAAGGGGCTTTAACATCGAAATTGTTAG
ATTCAAAAATTAGTAGATTACATGCCGTCGAATTGGATAAAGATTTAATTGACCTTTTAAATAATAAATTTAGAAATGAT
AAAAAGTTTTCACTTCAACAAGGCGATATCCTTTCAACAAATCTTGACTCCATAAATAAAAAAATTACAAAAGTGATTGC
AAATATTCCTTATAATATTACAGGTCCAATATTGGATATTTTTATTGGAAGATTAGGAATAGTTAGTAAGAATAATTACA
AGAAAATAATTTTCTTAATGCAGAAGGATGTTGTGGATAGGATTTTATCTAAAGAAAGTAGTAAGAATGCAGGAGCTATG
AGTGTGAGGATGCAACTTATCTCTAACATAAAAAGAATATGCGATGTGCCTCCATCATCTTTTAACCCACCTCCAAAAGT
ATTTTCTTCTTTGGTTGTTTTTGAACCATTGAGGCCTGAGAAACGATTAGATATTAAACTAGAGAGATATATAGATAAAC
TTCTTCAAATTTCATTTAATTCAAGAAGAAAAATGATTAGAAATACTCTTAATTCAATCCTCTCAGAGGATGAGATTAAG
AAATTAGCTGAATTATCTGAAATATGCTTTAATTCAAGACCTCAAGATATTTCAATTAATAAATGGATTAAGCTTGCAGA
GGGATGTATTAAAATAACAAATAAGAATTAG

Upstream 100 bases:

>100_bases
AAAAGTATTGAGTTTACCTAAATTCTTTTTTGATAGGCCCATACAATTTAATAAAGATATAAACTATATATTTAATTTAA
ACATAATATTTCTCTTTTGG

Downstream 100 bases:

>100_bases
TAAAATGACAAAATTATCTACAACTAAAATTTGTGTGAAATCACCTGCGAAGATAAACCTCCATCTTGAAATTATCGGAA
AAAGAGAGGATGGATATCAT

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNFKNHHQKKRFGQHWLVNNLILEKIKEVAELDEKDFILEIGPGKGALTSKLLDSKISRLHAVELDKDLIDLLNNKFRND
KKFSLQQGDILSTNLDSINKKITKVIANIPYNITGPILDIFIGRLGIVSKNNYKKIIFLMQKDVVDRILSKESSKNAGAM
SVRMQLISNIKRICDVPPSSFNPPPKVFSSLVVFEPLRPEKRLDIKLERYIDKLLQISFNSRRKMIRNTLNSILSEDEIK
KLAELSEICFNSRPQDISINKWIKLAEGCIKITNKN

Sequences:

>Translated_276_residues
MNFKNHHQKKRFGQHWLVNNLILEKIKEVAELDEKDFILEIGPGKGALTSKLLDSKISRLHAVELDKDLIDLLNNKFRND
KKFSLQQGDILSTNLDSINKKITKVIANIPYNITGPILDIFIGRLGIVSKNNYKKIIFLMQKDVVDRILSKESSKNAGAM
SVRMQLISNIKRICDVPPSSFNPPPKVFSSLVVFEPLRPEKRLDIKLERYIDKLLQISFNSRRKMIRNTLNSILSEDEIK
KLAELSEICFNSRPQDISINKWIKLAEGCIKITNKN
>Mature_276_residues
MNFKNHHQKKRFGQHWLVNNLILEKIKEVAELDEKDFILEIGPGKGALTSKLLDSKISRLHAVELDKDLIDLLNNKFRND
KKFSLQQGDILSTNLDSINKKITKVIANIPYNITGPILDIFIGRLGIVSKNNYKKIIFLMQKDVVDRILSKESSKNAGAM
SVRMQLISNIKRICDVPPSSFNPPPKVFSSLVVFEPLRPEKRLDIKLERYIDKLLQISFNSRRKMIRNTLNSILSEDEIK
KLAELSEICFNSRPQDISINKWIKLAEGCIKITNKN

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=235, Percent_Identity=30.6382978723404, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI156415992, Length=289, Percent_Identity=23.8754325259516, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1786236, Length=268, Percent_Identity=29.4776119402985, Blast_Score=130, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=260, Percent_Identity=26.1538461538462, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25141369, Length=294, Percent_Identity=25.5102040816327, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324989, Length=234, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21358017, Length=224, Percent_Identity=29.4642857142857, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21357273, Length=277, Percent_Identity=25.2707581227437, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 31723; Mature: 31723

Theoretical pI: Translated: 10.41; Mature: 10.41

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKNHHQKKRFGQHWLVNNLILEKIKEVAELDEKDFILEIGPGKGALTSKLLDSKISRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
HAVELDKDLIDLLNNKFRNDKKFSLQQGDILSTNLDSINKKITKVIANIPYNITGPILDI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
FIGRLGIVSKNNYKKIIFLMQKDVVDRILSKESSKNAGAMSVRMQLISNIKRICDVPPSS
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
FNPPPKVFSSLVVFEPLRPEKRLDIKLERYIDKLLQISFNSRRKMIRNTLNSILSEDEIK
CCCCHHHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
KLAELSEICFNSRPQDISINKWIKLAEGCIKITNKN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNFKNHHQKKRFGQHWLVNNLILEKIKEVAELDEKDFILEIGPGKGALTSKLLDSKISRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
HAVELDKDLIDLLNNKFRNDKKFSLQQGDILSTNLDSINKKITKVIANIPYNITGPILDI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
FIGRLGIVSKNNYKKIIFLMQKDVVDRILSKESSKNAGAMSVRMQLISNIKRICDVPPSS
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
FNPPPKVFSSLVVFEPLRPEKRLDIKLERYIDKLLQISFNSRRKMIRNTLNSILSEDEIK
CCCCHHHHHHHHHHCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
KLAELSEICFNSRPQDISINKWIKLAEGCIKITNKN
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]