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The map label for this gene is ispE

Identifier: 123966248

GI number: 123966248

Start: 908353

End: 909291

Strand: Reverse

Name: ispE

Synonym: P9515_10151

Alternate gene names: 123966248

Gene position: 909291-908353 (Counterclockwise)

Preceding gene: 123966249

Following gene: 123966247

Centisome position: 53.36

GC content: 27.48

Gene sequence:

>939_bases
ATGACAAAATTATCTACAACTAAAATTTGTGTGAAATCACCTGCGAAGATAAACCTCCATCTTGAAATTATCGGAAAAAG
AGAGGATGGATATCATGAATTAGCAATGATAATGCAGAATATTGATCTTTTTGACTATATAGAATTTGAGAATAATCAAA
TAGGTGATATTGAATTAAAGTCTAATAACAAAGATTTAAGTCTCAAGGATGATAACTTAATAATAAAAGCAGCAAATTAT
GTAAAAGATATATCAAAAAAAAAAGATTTAGGAGCTAATATCTTTCTTAAAAAAAATATTCCTATTGGTGCTGGTTTAGC
TGGAGGTTCCAGCAATGCAGCCGCAACAATAATTGGACTTAATAAATTATGGGATTTGGATCTTGATAACGAAACTATGC
TTTCACTTTCCTCAAAACTAGGATCAGATGTACCTTTTTTTATAAACGGAGGATGCCAATTTTGTTTTGGAAGGGGAGAA
ATTCTTGAAAATTATAATTCTAAATTTGATTATGGCGTAATACTCTTAAAAAATCCAAATATTTCAATCTCAACTGCAGA
TACTTACAAAAAATATAGTAAGGAATATTGCTCAAATTTTTTAACTGAATCTGAAAAAACAAATGATATCAGAAATGATT
TGAGAATAAACGGATTTAATGATCTTAATTCTTCAAATCAGATTATAAAAGTAAAAAATGATTTACAAATTATCGTTGAA
AAAGAAAATGATTCTGTAAAGAAGGCATTATATCTTCTCTCAAATTTGCAAAATTGTTTATCTTTTTCAATGAGTGGATC
TGGCCCAACATGTTTTGCTCTTTTTAAAGATATAAATAAGGCTAATGAAGTTTTTGAACAAAATCATAAAATGTTTAATA
ATAATGGATTTGAAGCGTGGGTCTGTAAATTAATTAATAGTGGCATAACCTTTTTGTAA

Upstream 100 bases:

>100_bases
TCTGAAATATGCTTTAATTCAAGACCTCAAGATATTTCAATTAATAAATGGATTAAGCTTGCAGAGGGATGTATTAAAAT
AACAAATAAGAATTAGTAAA

Downstream 100 bases:

>100_bases
AAACCTTTTTTTTCTATAGAAAATGAATAATGAAAAATCATCCAATATTAACGATGTTATTAAAAATCAAAATATCCCTG
AAAAAGGCCCCTTAAGTTTT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTKLSTTKICVKSPAKINLHLEIIGKREDGYHELAMIMQNIDLFDYIEFENNQIGDIELKSNNKDLSLKDDNLIIKAANY
VKDISKKKDLGANIFLKKNIPIGAGLAGGSSNAAATIIGLNKLWDLDLDNETMLSLSSKLGSDVPFFINGGCQFCFGRGE
ILENYNSKFDYGVILLKNPNISISTADTYKKYSKEYCSNFLTESEKTNDIRNDLRINGFNDLNSSNQIIKVKNDLQIIVE
KENDSVKKALYLLSNLQNCLSFSMSGSGPTCFALFKDINKANEVFEQNHKMFNNNGFEAWVCKLINSGITFL

Sequences:

>Translated_312_residues
MTKLSTTKICVKSPAKINLHLEIIGKREDGYHELAMIMQNIDLFDYIEFENNQIGDIELKSNNKDLSLKDDNLIIKAANY
VKDISKKKDLGANIFLKKNIPIGAGLAGGSSNAAATIIGLNKLWDLDLDNETMLSLSSKLGSDVPFFINGGCQFCFGRGE
ILENYNSKFDYGVILLKNPNISISTADTYKKYSKEYCSNFLTESEKTNDIRNDLRINGFNDLNSSNQIIKVKNDLQIIVE
KENDSVKKALYLLSNLQNCLSFSMSGSGPTCFALFKDINKANEVFEQNHKMFNNNGFEAWVCKLINSGITFL
>Mature_311_residues
TKLSTTKICVKSPAKINLHLEIIGKREDGYHELAMIMQNIDLFDYIEFENNQIGDIELKSNNKDLSLKDDNLIIKAANYV
KDISKKKDLGANIFLKKNIPIGAGLAGGSSNAAATIIGLNKLWDLDLDNETMLSLSSKLGSDVPFFINGGCQFCFGRGEI
LENYNSKFDYGVILLKNPNISISTADTYKKYSKEYCSNFLTESEKTNDIRNDLRINGFNDLNSSNQIIKVKNDLQIIVEK
ENDSVKKALYLLSNLQNCLSFSMSGSGPTCFALFKDINKANEVFEQNHKMFNNNGFEAWVCKLINSGITFL

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=293, Percent_Identity=32.0819112627986, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PROM5 (A2BWR1)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001011329.1
- ProteinModelPortal:   A2BWR1
- SMR:   A2BWR1
- STRING:   A2BWR1
- GeneID:   4719163
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_10151
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- ProtClustDB:   PRK02534
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34941; Mature: 34810

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 16-16 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLSTTKICVKSPAKINLHLEIIGKREDGYHELAMIMQNIDLFDYIEFENNQIGDIELK
CCCCCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEEC
SNNKDLSLKDDNLIIKAANYVKDISKKKDLGANIFLKKNIPIGAGLAGGSSNAAATIIGL
CCCCCEEEECCCEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEC
NKLWDLDLDNETMLSLSSKLGSDVPFFINGGCQFCFGRGEILENYNSKFDYGVILLKNPN
CCEEECCCCCHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHCCCCCCCEEEEEEECCC
ISISTADTYKKYSKEYCSNFLTESEKTNDIRNDLRINGFNDLNSSNQIIKVKNDLQIIVE
EEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEECCCCCCCCCCCEEEEECCEEEEEE
KENDSVKKALYLLSNLQNCLSFSMSGSGPTCFALFKDINKANEVFEQNHKMFNNNGFEAW
CCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VCKLINSGITFL
HHHHHHCCCCCC
>Mature Secondary Structure 
TKLSTTKICVKSPAKINLHLEIIGKREDGYHELAMIMQNIDLFDYIEFENNQIGDIELK
CCCCCEEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCEEEEEEEC
SNNKDLSLKDDNLIIKAANYVKDISKKKDLGANIFLKKNIPIGAGLAGGSSNAAATIIGL
CCCCCEEEECCCEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEC
NKLWDLDLDNETMLSLSSKLGSDVPFFINGGCQFCFGRGEILENYNSKFDYGVILLKNPN
CCEEECCCCCHHHHHHHHHHCCCCCEEEECCEEEEECCHHHHHCCCCCCCEEEEEEECCC
ISISTADTYKKYSKEYCSNFLTESEKTNDIRNDLRINGFNDLNSSNQIIKVKNDLQIIVE
EEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHCCEECCCCCCCCCCCEEEEECCEEEEEE
KENDSVKKALYLLSNLQNCLSFSMSGSGPTCFALFKDINKANEVFEQNHKMFNNNGFEAW
CCCCHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
VCKLINSGITFL
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA