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The map label for this gene is dnaG [H]

Identifier: 123966255

GI number: 123966255

Start: 913202

End: 915238

Strand: Reverse

Name: dnaG [H]

Synonym: P9515_10221

Alternate gene names: 123966255

Gene position: 915238-913202 (Counterclockwise)

Preceding gene: 123966261

Following gene: 123966249

Centisome position: 53.71

GC content: 29.11

Gene sequence:

>2037_bases
ATGGGACCTTCAATACATCCAAGAACTATCCAGGAGGTTAAAGATAAGGCAGATATTGTAGATGTTATTTCTGAACATAT
TGTCTTAAAGAAAAAGGGAAAAGAATTTGTTGGGATATGCCCTTTTCATGATGATAATAAGCCGTCCATGACAGTTTCTC
CTGCGAAACAATTTTATTATTGTTTCTCATGTGGAGCTGGAGGTAATTCTATTAAATTTTTAATGGAGTTTACTCGTAAT
AACTTTACAGACGTAGTCCTCTCTCTCGCAAAAAAGAATGATATAAATGTTATAAATGTTGATGGCCCTCAACAAGAGGT
TTATAAAAAACAATTATCTAGAAGAGAAGAACTTTATAAGATTCTTCGAGTTACAAAAGATTGGTTTGTTTCACAATTAA
ATAATTCCTTGGGTAAAGAGGCCCTAAACTATTTAACTTCGCAAAGAAATTTAAATATTAAAAATATTAATGATTTTCAA
TTAGGTTTTGCTCCAAATTCATGGAACGATTTATTCAATTATCTCTCAAAAGTAGAGAAATTCCCTTTGAAGTTAATTTT
AAGTGCAGGTCTTGTTGTCTCTAAAGAAAATTCTGATAAGGTCTATGATCGCTTTAGAAATAGATTGATAGTTCCTATTT
TTGATATGCAAGGGCGAGTGGTTGCTTTTGGAGGAAGATCTCTTGATGGTCAAGAACCAAAATATTTAAATTCTCCCGAA
TCGGAAGTTTTTGAGAAGGGTAAAATGCTATTTGCGTTTGATAAAGCATCCAGCAATATTAGAAAAAGTGATAAGGCTGT
TGTAGTGGAAGGTTATTTTGATGTTATTTCACTTCATGCAAAAGGCATTACGAATTCTGTTGCTTCTCTCGGAACAGCGT
TAAATAAATATCAAATTTCCCAACTTTGTAGATGTACCGATAGTAAAAATATAATAATTAATTTTGATTCTGATAATGCA
GGGATATTAGCTACTAAAAGAGTTATAAACGAGGTCGAAATCTTATCACTTCATGACCAAATAAATCTAAAAATACTCCA
AATAAAATCATTTAAAGATCCTGATGAGTATCTTAAAAGAAACACTCCAGAAGATTATATAAATTTAATAGATAACGCTT
CTTTTTGGATAGATTGGGAAATTGATCAAATTTTTGAGAATAAGGATTTATCTAAATCTGATATTTTTCAGAACGTTATT
TCCTCTTTAGTTAAGTTATTAAGCAAACTTCCTCAATCTGCTACAAGAACTCACTATTTGCAAAAAGTCTCTGAAAGATT
AAGTATGGGGCAAGCGAGATTAGCTATCAAATTTGAAGAAGACTTAAGAAAACAAGTTAAAGGGTTTCGTTGGCATGGAA
GATCAAAAAAATTTGAACAGCCAAATGAAATAAGTCAACGTGAAAAAAATGAATCAGAGATTATTTATTACTATATACAT
TGTCCTGAATACAGACTTTTTATTCGTGAAGAATTGCTTAAAAGAGAAATAGAAAAATTTAACACTGAATATATTCGTCT
CATTTGGGACTCTATTTCAACAATAGAAGGAAATAATTTTGGTTCAAATTATTTAGATGATTTGAGAAATTCAAATAATC
AAAAACTTAATAATGACTTTATTTCTCTAAATTTAATTTCACTATTGCCTGATCACTTAGCTCTTAATAATTTAGAAGTC
TTAAATAAAATAAATACTTTTATTAATCCAGATGAATTATTTTTAACTACTTTAAAAAATCCTAAACATAATCTTCTCGG
AACCTTGTCTCTTTTAGAAAGATACAAATCATTAAAAAGGTGTAGACATCTTATTGAATCTTGGGGTTCTCAAAGACTTA
AAACTTTAGAAAACTGTATTTCTATTTTAATCGATAATTCTTTATTAGAATCTTCAGAATCGAATAAAGAGATTGATGAT
GTCTTTAATGATTTAAACTCTGATGCTATAAAATTTCAAGAATTATATTATCTAGAAAGACAGCACATTAATCTTCTTGA
TAAACAACGGTGTGGAAATTTTTCTTCTAATCAATAA

Upstream 100 bases:

>100_bases
CATTACCAAAAATAATTTTATTGTGAAATTAAGTAGATTCTTTTAAAGGATTGACTTATAACATAATGATAATTAGGACA
TCTTTTTAGAAAATATATAA

Downstream 100 bases:

>100_bases
CTTTAAACGAATTTATAAACAATAGCTTTTAATTATCTCTCTTACTTTTCTTGGCTTGTCTTCCATAACATAAGCCTCTA
ATTCTTTTTCATAATTTCCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MGPSIHPRTIQEVKDKADIVDVISEHIVLKKKGKEFVGICPFHDDNKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFTRN
NFTDVVLSLAKKNDINVINVDGPQQEVYKKQLSRREELYKILRVTKDWFVSQLNNSLGKEALNYLTSQRNLNIKNINDFQ
LGFAPNSWNDLFNYLSKVEKFPLKLILSAGLVVSKENSDKVYDRFRNRLIVPIFDMQGRVVAFGGRSLDGQEPKYLNSPE
SEVFEKGKMLFAFDKASSNIRKSDKAVVVEGYFDVISLHAKGITNSVASLGTALNKYQISQLCRCTDSKNIIINFDSDNA
GILATKRVINEVEILSLHDQINLKILQIKSFKDPDEYLKRNTPEDYINLIDNASFWIDWEIDQIFENKDLSKSDIFQNVI
SSLVKLLSKLPQSATRTHYLQKVSERLSMGQARLAIKFEEDLRKQVKGFRWHGRSKKFEQPNEISQREKNESEIIYYYIH
CPEYRLFIREELLKREIEKFNTEYIRLIWDSISTIEGNNFGSNYLDDLRNSNNQKLNNDFISLNLISLLPDHLALNNLEV
LNKINTFINPDELFLTTLKNPKHNLLGTLSLLERYKSLKRCRHLIESWGSQRLKTLENCISILIDNSLLESSESNKEIDD
VFNDLNSDAIKFQELYYLERQHINLLDKQRCGNFSSNQ

Sequences:

>Translated_678_residues
MGPSIHPRTIQEVKDKADIVDVISEHIVLKKKGKEFVGICPFHDDNKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFTRN
NFTDVVLSLAKKNDINVINVDGPQQEVYKKQLSRREELYKILRVTKDWFVSQLNNSLGKEALNYLTSQRNLNIKNINDFQ
LGFAPNSWNDLFNYLSKVEKFPLKLILSAGLVVSKENSDKVYDRFRNRLIVPIFDMQGRVVAFGGRSLDGQEPKYLNSPE
SEVFEKGKMLFAFDKASSNIRKSDKAVVVEGYFDVISLHAKGITNSVASLGTALNKYQISQLCRCTDSKNIIINFDSDNA
GILATKRVINEVEILSLHDQINLKILQIKSFKDPDEYLKRNTPEDYINLIDNASFWIDWEIDQIFENKDLSKSDIFQNVI
SSLVKLLSKLPQSATRTHYLQKVSERLSMGQARLAIKFEEDLRKQVKGFRWHGRSKKFEQPNEISQREKNESEIIYYYIH
CPEYRLFIREELLKREIEKFNTEYIRLIWDSISTIEGNNFGSNYLDDLRNSNNQKLNNDFISLNLISLLPDHLALNNLEV
LNKINTFINPDELFLTTLKNPKHNLLGTLSLLERYKSLKRCRHLIESWGSQRLKTLENCISILIDNSLLESSESNKEIDD
VFNDLNSDAIKFQELYYLERQHINLLDKQRCGNFSSNQ
>Mature_677_residues
GPSIHPRTIQEVKDKADIVDVISEHIVLKKKGKEFVGICPFHDDNKPSMTVSPAKQFYYCFSCGAGGNSIKFLMEFTRNN
FTDVVLSLAKKNDINVINVDGPQQEVYKKQLSRREELYKILRVTKDWFVSQLNNSLGKEALNYLTSQRNLNIKNINDFQL
GFAPNSWNDLFNYLSKVEKFPLKLILSAGLVVSKENSDKVYDRFRNRLIVPIFDMQGRVVAFGGRSLDGQEPKYLNSPES
EVFEKGKMLFAFDKASSNIRKSDKAVVVEGYFDVISLHAKGITNSVASLGTALNKYQISQLCRCTDSKNIIINFDSDNAG
ILATKRVINEVEILSLHDQINLKILQIKSFKDPDEYLKRNTPEDYINLIDNASFWIDWEIDQIFENKDLSKSDIFQNVIS
SLVKLLSKLPQSATRTHYLQKVSERLSMGQARLAIKFEEDLRKQVKGFRWHGRSKKFEQPNEISQREKNESEIIYYYIHC
PEYRLFIREELLKREIEKFNTEYIRLIWDSISTIEGNNFGSNYLDDLRNSNNQKLNNDFISLNLISLLPDHLALNNLEVL
NKINTFINPDELFLTTLKNPKHNLLGTLSLLERYKSLKRCRHLIESWGSQRLKTLENCISILIDNSLLESSESNKEIDDV
FNDLNSDAIKFQELYYLERQHINLLDKQRCGNFSSNQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=428, Percent_Identity=33.6448598130841, Blast_Score=245, Evalue=8e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 78453; Mature: 78322

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSIHPRTIQEVKDKADIVDVISEHIVLKKKGKEFVGICPFHDDNKPSMTVSPAKQFYY
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEECCHHHEEE
CFSCGAGGNSIKFLMEFTRNNFTDVVLSLAKKNDINVINVDGPQQEVYKKQLSRREELYK
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
ILRVTKDWFVSQLNNSLGKEALNYLTSQRNLNIKNINDFQLGFAPNSWNDLFNYLSKVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
FPLKLILSAGLVVSKENSDKVYDRFRNRLIVPIFDMQGRVVAFGGRSLDGQEPKYLNSPE
CCHHHHHHCCEEEECCCCHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCH
SEVFEKGKMLFAFDKASSNIRKSDKAVVVEGYFDVISLHAKGITNSVASLGTALNKYQIS
HHHHHCCCEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QLCRCTDSKNIIINFDSDNAGILATKRVINEVEILSLHDQINLKILQIKSFKDPDEYLKR
HHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHC
NTPEDYINLIDNASFWIDWEIDQIFENKDLSKSDIFQNVISSLVKLLSKLPQSATRTHYL
CCCHHHHHHHCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QKVSERLSMGQARLAIKFEEDLRKQVKGFRWHGRSKKFEQPNEISQREKNESEIIYYYIH
HHHHHHHCCCCCEEEEEEHHHHHHHHHCCEECCCCCCCCCCHHHHHHCCCCCCEEEEEEE
CPEYRLFIREELLKREIEKFNTEYIRLIWDSISTIEGNNFGSNYLDDLRNSNNQKLNNDF
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCE
ISLNLISLLPDHLALNNLEVLNKINTFINPDELFLTTLKNPKHNLLGTLSLLERYKSLKR
EEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHH
CRHLIESWGSQRLKTLENCISILIDNSLLESSESNKEIDDVFNDLNSDAIKFQELYYLER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
QHINLLDKQRCGNFSSNQ
HHHCHHHHHHCCCCCCCC
>Mature Secondary Structure 
GPSIHPRTIQEVKDKADIVDVISEHIVLKKKGKEFVGICPFHDDNKPSMTVSPAKQFYY
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCCCCCCCCEEECCHHHEEE
CFSCGAGGNSIKFLMEFTRNNFTDVVLSLAKKNDINVINVDGPQQEVYKKQLSRREELYK
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
ILRVTKDWFVSQLNNSLGKEALNYLTSQRNLNIKNINDFQLGFAPNSWNDLFNYLSKVEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHH
FPLKLILSAGLVVSKENSDKVYDRFRNRLIVPIFDMQGRVVAFGGRSLDGQEPKYLNSPE
CCHHHHHHCCEEEECCCCHHHHHHHHCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCH
SEVFEKGKMLFAFDKASSNIRKSDKAVVVEGYFDVISLHAKGITNSVASLGTALNKYQIS
HHHHHCCCEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QLCRCTDSKNIIINFDSDNAGILATKRVINEVEILSLHDQINLKILQIKSFKDPDEYLKR
HHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHEEEECCCCCEEEEEEECCCCHHHHHHC
NTPEDYINLIDNASFWIDWEIDQIFENKDLSKSDIFQNVISSLVKLLSKLPQSATRTHYL
CCCHHHHHHHCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
QKVSERLSMGQARLAIKFEEDLRKQVKGFRWHGRSKKFEQPNEISQREKNESEIIYYYIH
HHHHHHHCCCCCEEEEEEHHHHHHHHHCCEECCCCCCCCCCHHHHHHCCCCCCEEEEEEE
CPEYRLFIREELLKREIEKFNTEYIRLIWDSISTIEGNNFGSNYLDDLRNSNNQKLNNDF
CCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCE
ISLNLISLLPDHLALNNLEVLNKINTFINPDELFLTTLKNPKHNLLGTLSLLERYKSLKR
EEEEHHHHCCHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHHHH
CRHLIESWGSQRLKTLENCISILIDNSLLESSESNKEIDDVFNDLNSDAIKFQELYYLER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHH
QHINLLDKQRCGNFSSNQ
HHHCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA