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The map label for this gene is yafV [C]

Identifier: 123965915

GI number: 123965915

Start: 616977

End: 617804

Strand: Direct

Name: yafV [C]

Synonym: P9515_06801

Alternate gene names: 123965915

Gene position: 616977-617804 (Clockwise)

Preceding gene: 123965914

Following gene: 123965916

Centisome position: 36.2

GC content: 39.61

Gene sequence:

>828_bases
TTGACTGATTTTTTAGTTGCCGCTTTACAAATAACGAGTACTTCAAATGTTGAAACAAATTTTGCTGAAGCTGAGGAGCA
GATTGAATTAGCCTCTAGAAGGGGTGCAGAATTAATTGGATTACCTGAAAATTTTGCATTCCTAGGAGAAGATAATGAAA
AATTAAGGTTGGCCTCTGAATTGGCAATAAAGTGTACTAATTTTTTAAAAACGATGTCTCAGCGATATCAAGTCTTTCTT
TTGGGTGGTGGTTATCCCGTTCCTGCAGGAGATAATAATCATACTTTTAACAGATCAGCCCTTTTTGGGAAAGATGGACA
GGTTTTGGCCAAATATGACAAAATTCACCTTTTTGATGTTGATTTGCCCGATGGTAATTTATACAAAGAATCATCAACTA
TCCTATCAGGTAAAGAACATCCTCCAGTCGTAGACGTGCCGGGACTTTGCAAGATAGGGCTTTCTATTTGTTACGATGTA
AGATTTCCCGAACTCTACAGAAACCTCTCTTTGAATGGTGCAGAACTCATAATGATCCCAGCAGCTTTTACAGCCTTCAC
AGGTAAAGATCATTGGCAAATTCTACTTCAAGCAAGAGCGATAGAAAATACAGCTTATGTAGTAGCGCCTGCTCAAACAG
GTATTCACTACGGGAGAAGGCAAAGTCATGGTCATGCCATGGTAATAGACCCATGGGGTACAGTTTTGTCTGATGCGGGG
AAAACACAGGGGGCAGCAATAGCTCCCGCTGATAAAGAAAGAGTAAAAAAGATAAGAGAGCAGATGCCAAGCTTAAAACA
TAGAAAAACGAAACTTTTCGCGGAATGA

Upstream 100 bases:

>100_bases
TTGTTCTGAACTTGATTGTTTAGATATTGTTCCTACTCAATTTGAGAGAGGTGTAATTCGTGCCTCATGATTTGCAAATT
TATTATCTTGGAGTTAAATC

Downstream 100 bases:

>100_bases
TGTCAAAGTTTTTTTTTAATAAACTTTTTCGCTGCCTATCAATATTTTTACTTTTTAATTCAAGTATTTCTCCAGCCAGA
TCATCTAGTGCACTAGCTGC

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDFLVAALQITSTSNVETNFAEAEEQIELASRRGAELIGLPENFAFLGEDNEKLRLASELAIKCTNFLKTMSQRYQVFL
LGGGYPVPAGDNNHTFNRSALFGKDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGKEHPPVVDVPGLCKIGLSICYDV
RFPELYRNLSLNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKERVKKIREQMPSLKHRKTKLFAE

Sequences:

>Translated_275_residues
MTDFLVAALQITSTSNVETNFAEAEEQIELASRRGAELIGLPENFAFLGEDNEKLRLASELAIKCTNFLKTMSQRYQVFL
LGGGYPVPAGDNNHTFNRSALFGKDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGKEHPPVVDVPGLCKIGLSICYDV
RFPELYRNLSLNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKERVKKIREQMPSLKHRKTKLFAE
>Mature_274_residues
TDFLVAALQITSTSNVETNFAEAEEQIELASRRGAELIGLPENFAFLGEDNEKLRLASELAIKCTNFLKTMSQRYQVFLL
GGGYPVPAGDNNHTFNRSALFGKDGQVLAKYDKIHLFDVDLPDGNLYKESSTILSGKEHPPVVDVPGLCKIGLSICYDVR
FPELYRNLSLNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAGK
TQGAAIAPADKERVKKIREQMPSLKHRKTKLFAE

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=273, Percent_Identity=38.8278388278388, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI5031947, Length=273, Percent_Identity=38.8278388278388, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI297632350, Length=273, Percent_Identity=38.8278388278388, Blast_Score=194, Evalue=7e-50,
Organism=Homo sapiens, GI9910460, Length=274, Percent_Identity=33.2116788321168, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=36.7875647668394, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1786412, Length=236, Percent_Identity=30.5084745762712, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=271, Percent_Identity=40.2214022140221, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6322335, Length=301, Percent_Identity=32.2259136212625, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=283, Percent_Identity=31.095406360424, Blast_Score=144, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17933642, Length=271, Percent_Identity=35.0553505535055, Blast_Score=160, Evalue=6e-40,
Organism=Drosophila melanogaster, GI21355835, Length=273, Percent_Identity=32.967032967033, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30361; Mature: 30230

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLVAALQITSTSNVETNFAEAEEQIELASRRGAELIGLPENFAFLGEDNEKLRLASE
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCHHHHHHH
LAIKCTNFLKTMSQRYQVFLLGGGYPVPAGDNNHTFNRSALFGKDGQVLAKYDKIHLFDV
HHHHHHHHHHHHHCCEEEEEEECCEECCCCCCCCCCCCCEEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGKEHPPVVDVPGLCKIGLSICYDVRFPELYRNLSLNGAELIMIP
ECCCCCCEECCCCEECCCCCCCEECCCCHHHHHHHHEEECCCHHHHHCCCCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCCEEEEEEEEEECCEEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKERVKKIREQMPSLKHRKTKLFAE
CCCCCEECCCCHHHHHHHHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
TDFLVAALQITSTSNVETNFAEAEEQIELASRRGAELIGLPENFAFLGEDNEKLRLASE
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCHHHHHHH
LAIKCTNFLKTMSQRYQVFLLGGGYPVPAGDNNHTFNRSALFGKDGQVLAKYDKIHLFDV
HHHHHHHHHHHHHCCEEEEEEECCEECCCCCCCCCCCCCEEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGKEHPPVVDVPGLCKIGLSICYDVRFPELYRNLSLNGAELIMIP
ECCCCCCEECCCCEECCCCCCCEECCCCHHHHHHHHEEECCCHHHHHCCCCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCCEEEEEEEEEECCEEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKERVKKIREQMPSLKHRKTKLFAE
CCCCCEECCCCHHHHHHHHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]