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The map label for this gene is amiC [H]

Identifier: 123965916

GI number: 123965916

Start: 617801

End: 618895

Strand: Direct

Name: amiC [H]

Synonym: P9515_06811

Alternate gene names: 123965916

Gene position: 617801-618895 (Clockwise)

Preceding gene: 123965915

Following gene: 123965917

Centisome position: 36.25

GC content: 33.06

Gene sequence:

>1095_bases
ATGATGTCAAAGTTTTTTTTTAATAAACTTTTTCGCTGCCTATCAATATTTTTACTTTTTAATTCAAGTATTTCTCCAGC
CAGATCATCTAGTGCACTAGCTGCATGGAGTTTAAATTCTAATGGAGTTTTAGAATTAAGGACTAAATCAAATTCAAAAT
TAAAGGCCTACTTTCAAAAAGGAGGGAAAAATTTTGGGGATAGATTTTGGATAGATTTCCCTGGGGAACTTCAAACTCCG
AGAACTATAGAGGGAAATGGTCCAATAAAAGAAATTAGATTGGGCAAACCAATTAAAGGCAAAACTAGATTAGTAGTTGA
ATTTTCTGCTGATAATAATAATTTGAAACCTCTTAGTTGGAGGCTAGTAGGTATAGATCAAAACACATGGAAAATTAAAT
TATTTTCTTTACCAAAAAATTCATTTCAAACAATTGGTGAAGGTCTTGTTAGTAAATCGAGAATTAATCTTAAGGCTAAT
AAAAAGCTAATTAAACCAAGAAAAACAAATTATGAGTTTTTGCAATTGCCGGATATTGAGAGAAATAAGTTTTATGTTGT
TTTAGATCCAGGCCATGGTGGCCCAGATCCAGGAGCAATCGGTATAGGGGGGGTAAAAGAAGCAGATGTGGTCCTTGATG
TTTCTAAAAGAGTTAGAAATTTATTATCTAAAAAAGGTGTAAATGTAAGAATGACGAGAAATAAAGAAGTTGATTTGGAT
TTACCTCCAAGAGTCTCAATAGCTAATAGGACTAATGCTGATGTTTTTGTGAGCATTCACGCTAATGCCTCAAGAGGTAA
AAGAAGAGATATTAATGGATTAGAAACTTTTTATTACACGGGATGGAGGGGCAGACTATTAGCCAAAAAAATTCAAAAAC
AAATCCTTAAAGTTTCACCTGGAAGTCCAGATAGAGGCGTAAGACAGGGGAGATTTTTCGTTATTAAAAATACTAGAATG
CCAGCAGTTCTTGTAGAAATAGGTTTTTTAACAGGAAGATTAGATGCGAGAAGGTTAGAAAAATCTATTCATCGTGAAAG
AATAGCTTATGCAATTACAAAAGGTATTCTTGAATATCTTTCTAAGGCAGGGTGA

Upstream 100 bases:

>100_bases
CACAGGGGGCAGCAATAGCTCCCGCTGATAAAGAAAGAGTAAAAAAGATAAGAGAGCAGATGCCAAGCTTAAAACATAGA
AAAACGAAACTTTTCGCGGA

Downstream 100 bases:

>100_bases
AAGTTAAAATAGGAATTTTTGACAGTGGAATAGGAGGGTTTACGATCCTTGATTATTTACTTAAGACTCGCAAGGATCTT
GAGATTTTTTACTTGGCAGA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MMSKFFFNKLFRCLSIFLLFNSSISPARSSSALAAWSLNSNGVLELRTKSNSKLKAYFQKGGKNFGDRFWIDFPGELQTP
RTIEGNGPIKEIRLGKPIKGKTRLVVEFSADNNNLKPLSWRLVGIDQNTWKIKLFSLPKNSFQTIGEGLVSKSRINLKAN
KKLIKPRKTNYEFLQLPDIERNKFYVVLDPGHGGPDPGAIGIGGVKEADVVLDVSKRVRNLLSKKGVNVRMTRNKEVDLD
LPPRVSIANRTNADVFVSIHANASRGKRRDINGLETFYYTGWRGRLLAKKIQKQILKVSPGSPDRGVRQGRFFVIKNTRM
PAVLVEIGFLTGRLDARRLEKSIHRERIAYAITKGILEYLSKAG

Sequences:

>Translated_364_residues
MMSKFFFNKLFRCLSIFLLFNSSISPARSSSALAAWSLNSNGVLELRTKSNSKLKAYFQKGGKNFGDRFWIDFPGELQTP
RTIEGNGPIKEIRLGKPIKGKTRLVVEFSADNNNLKPLSWRLVGIDQNTWKIKLFSLPKNSFQTIGEGLVSKSRINLKAN
KKLIKPRKTNYEFLQLPDIERNKFYVVLDPGHGGPDPGAIGIGGVKEADVVLDVSKRVRNLLSKKGVNVRMTRNKEVDLD
LPPRVSIANRTNADVFVSIHANASRGKRRDINGLETFYYTGWRGRLLAKKIQKQILKVSPGSPDRGVRQGRFFVIKNTRM
PAVLVEIGFLTGRLDARRLEKSIHRERIAYAITKGILEYLSKAG
>Mature_364_residues
MMSKFFFNKLFRCLSIFLLFNSSISPARSSSALAAWSLNSNGVLELRTKSNSKLKAYFQKGGKNFGDRFWIDFPGELQTP
RTIEGNGPIKEIRLGKPIKGKTRLVVEFSADNNNLKPLSWRLVGIDQNTWKIKLFSLPKNSFQTIGEGLVSKSRINLKAN
KKLIKPRKTNYEFLQLPDIERNKFYVVLDPGHGGPDPGAIGIGGVKEADVVLDVSKRVRNLLSKKGVNVRMTRNKEVDLD
LPPRVSIANRTNADVFVSIHANASRGKRRDINGLETFYYTGWRGRLLAKKIQKQILKVSPGSPDRGVRQGRFFVIKNTRM
PAVLVEIGFLTGRLDARRLEKSIHRERIAYAITKGILEYLSKAG

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=221, Percent_Identity=32.1266968325792, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87082163, Length=220, Percent_Identity=29.5454545454545, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790611, Length=87, Percent_Identity=39.0804597701149, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41016; Mature: 41016

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSKFFFNKLFRCLSIFLLFNSSISPARSSSALAAWSLNSNGVLELRTKSNSKLKAYFQK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHH
GGKNFGDRFWIDFPGELQTPRTIEGNGPIKEIRLGKPIKGKTRLVVEFSADNNNLKPLSW
CCCCCCCEEEEECCCCCCCCCEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCEEEE
RLVGIDQNTWKIKLFSLPKNSFQTIGEGLVSKSRINLKANKKLIKPRKTNYEFLQLPDIE
EEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCEEEECCCCC
RNKFYVVLDPGHGGPDPGAIGIGGVKEADVVLDVSKRVRNLLSKKGVNVRMTRNKEVDLD
CCEEEEEEECCCCCCCCCCEECCCCCCCCEEEEHHHHHHHHHHHCCCEEEEECCCEECCC
LPPRVSIANRTNADVFVSIHANASRGKRRDINGLETFYYTGWRGRLLAKKIQKQILKVSP
CCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHEECCC
GSPDRGVRQGRFFVIKNTRMPAVLVEIGFLTGRLDARRLEKSIHRERIAYAITKGILEYL
CCCCCCCCCCEEEEEECCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SKAG
HHCC
>Mature Secondary Structure
MMSKFFFNKLFRCLSIFLLFNSSISPARSSSALAAWSLNSNGVLELRTKSNSKLKAYFQK
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCHHHHHHHH
GGKNFGDRFWIDFPGELQTPRTIEGNGPIKEIRLGKPIKGKTRLVVEFSADNNNLKPLSW
CCCCCCCEEEEECCCCCCCCCEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCEEEE
RLVGIDQNTWKIKLFSLPKNSFQTIGEGLVSKSRINLKANKKLIKPRKTNYEFLQLPDIE
EEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCEECCCCCCCCEEEECCCCC
RNKFYVVLDPGHGGPDPGAIGIGGVKEADVVLDVSKRVRNLLSKKGVNVRMTRNKEVDLD
CCEEEEEEECCCCCCCCCCEECCCCCCCCEEEEHHHHHHHHHHHCCCEEEEECCCEECCC
LPPRVSIANRTNADVFVSIHANASRGKRRDINGLETFYYTGWRGRLLAKKIQKQILKVSP
CCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHEECCC
GSPDRGVRQGRFFVIKNTRMPAVLVEIGFLTGRLDARRLEKSIHRERIAYAITKGILEYL
CCCCCCCCCCEEEEEECCCCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SKAG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]