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The map label for this gene is murF [H]

Identifier: 123965910

GI number: 123965910

Start: 611107

End: 612477

Strand: Direct

Name: murF [H]

Synonym: P9515_06751

Alternate gene names: 123965910

Gene position: 611107-612477 (Clockwise)

Preceding gene: 123965909

Following gene: 123965914

Centisome position: 35.86

GC content: 26.33

Gene sequence:

>1371_bases
ATGGATTTTTCTGTATGTAATATAAATACAATTTTAGGGAATTTAAAAAATTCGGAGAATCTTAAAAATCAATCTCAAAC
TTTTAAAAATATAAGCTTAGATAGTCGGAATATCTTAAATAATGATCTTTTTATCGCAATAAAAGGAAAAAATTTCGATG
GACATGATTTTCTCAAAGAGGTCTTAAAAAAAGGCGTAAAAGCAGTTGTTATTCGAGACGGGATGCAGCATTTATTACCT
GAAAAGTTTCCTTATTGGCTTGTACCTAATACTCTAGAATCTTTCCAAAAATTAGCTTTACATAAGAGAAGAACATTAAA
TATACCTGTTGTAGGAATTACAGGATCAGTCGGGAAAACAACAACAAAAGAAATGATGGGAGAAGTGTTAAAGCAACTAG
GAAAAATCAAATTAACCCAATTAAACTATAATAATGAAATTGGAGTAGGCCGTACTATCCTTGATTCGGATTTCAAAGAT
AAAATTTTGATAATCGAGATGGGAATGAGAGGACTAGGACAGATTGAAAATTTATCTAAATTTTCTGAACCTGATATTGC
AGTTATTACAAATATTGGAAGTTCGCATATCGGAATTTTAGGCTCAAAAGAAAATATTACTTATGCGAAATGTGAAATAA
CCAAGTATTTAAAACCCAGTGGAGTCGTAATTATTCCTGCTAATGATTTATTCTTAGAAGAAACTTTAAAAAAAAATTGG
AGTGGGAGAATTATTAAGGTAGACCTCTTAGATATAAATCAAAAAGTTGATAGTATTAATAAAAAAAATTTAATAGGATT
TTTTAATCGATCTAATAATTCGATCATCATAGAAGATAAGCTTTTTGAAATATCACTTAAAGGTTTTCATAATGCCTTTA
ATTTCTTATTTGTATATGCAGTTGCTAAAGAGTTTGGAATAGAATTCAAAGATACTAATAAATTCAATTTTCTTACTTTA
GATGGAAGGAATAAAATTATTAAATCAAAAAAAACTGTCATATACGATGAAACTTATAACGCATCCCCTGAATCCTTAAA
AGCATGTATTATAAATTTATTAAATAATCCAAATAATCATTTTCTAATTTTTGGGAGTATACAAGAATTAGGATCTAAAA
GTAAAAAATATCATAAGGAACTTTTTGATTTTATAGTTAAATCAGATATAAAAAAATGTATATTTATATGTGATAGAAAT
GATGAGATCTATTATGCCGATTATTTAAAAAAAAGTACTAAATTTATATTTTTTAATGACGTAAATAAAGTTGGTATTAC
TATAAATAAATATACAAAAAAAGGAGATTTTATCTTGGTTAAAGGTAGCCGAAATTGGCAGCTTGAAAGTATTCTTAAAT
CAATTGATTAA

Upstream 100 bases:

>100_bases
GCGGCATTAAGGAGCCATCACCAGATATTACTGAAATTGAAAAGTTTTCTTATGGTCAATCTGCTGATCCTTCTTTAAAA
AAAATATGATTTAATTTTTA

Downstream 100 bases:

>100_bases
TTTTTGAATTTAATTTTTTTTCCAATTTTCAATATTTACTTGTTTGGTTCTTGCAATAGCTAAAGAATTATTTTGAGAAT
CTTTAGTAATTACTGAGCCT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDFSVCNINTILGNLKNSENLKNQSQTFKNISLDSRNILNNDLFIAIKGKNFDGHDFLKEVLKKGVKAVVIRDGMQHLLP
EKFPYWLVPNTLESFQKLALHKRRTLNIPVVGITGSVGKTTTKEMMGEVLKQLGKIKLTQLNYNNEIGVGRTILDSDFKD
KILIIEMGMRGLGQIENLSKFSEPDIAVITNIGSSHIGILGSKENITYAKCEITKYLKPSGVVIIPANDLFLEETLKKNW
SGRIIKVDLLDINQKVDSINKKNLIGFFNRSNNSIIIEDKLFEISLKGFHNAFNFLFVYAVAKEFGIEFKDTNKFNFLTL
DGRNKIIKSKKTVIYDETYNASPESLKACIINLLNNPNNHFLIFGSIQELGSKSKKYHKELFDFIVKSDIKKCIFICDRN
DEIYYADYLKKSTKFIFFNDVNKVGITINKYTKKGDFILVKGSRNWQLESILKSID

Sequences:

>Translated_456_residues
MDFSVCNINTILGNLKNSENLKNQSQTFKNISLDSRNILNNDLFIAIKGKNFDGHDFLKEVLKKGVKAVVIRDGMQHLLP
EKFPYWLVPNTLESFQKLALHKRRTLNIPVVGITGSVGKTTTKEMMGEVLKQLGKIKLTQLNYNNEIGVGRTILDSDFKD
KILIIEMGMRGLGQIENLSKFSEPDIAVITNIGSSHIGILGSKENITYAKCEITKYLKPSGVVIIPANDLFLEETLKKNW
SGRIIKVDLLDINQKVDSINKKNLIGFFNRSNNSIIIEDKLFEISLKGFHNAFNFLFVYAVAKEFGIEFKDTNKFNFLTL
DGRNKIIKSKKTVIYDETYNASPESLKACIINLLNNPNNHFLIFGSIQELGSKSKKYHKELFDFIVKSDIKKCIFICDRN
DEIYYADYLKKSTKFIFFNDVNKVGITINKYTKKGDFILVKGSRNWQLESILKSID
>Mature_456_residues
MDFSVCNINTILGNLKNSENLKNQSQTFKNISLDSRNILNNDLFIAIKGKNFDGHDFLKEVLKKGVKAVVIRDGMQHLLP
EKFPYWLVPNTLESFQKLALHKRRTLNIPVVGITGSVGKTTTKEMMGEVLKQLGKIKLTQLNYNNEIGVGRTILDSDFKD
KILIIEMGMRGLGQIENLSKFSEPDIAVITNIGSSHIGILGSKENITYAKCEITKYLKPSGVVIIPANDLFLEETLKKNW
SGRIIKVDLLDINQKVDSINKKNLIGFFNRSNNSIIIEDKLFEISLKGFHNAFNFLFVYAVAKEFGIEFKDTNKFNFLTL
DGRNKIIKSKKTVIYDETYNASPESLKACIINLLNNPNNHFLIFGSIQELGSKSKKYHKELFDFIVKSDIKKCIFICDRN
DEIYYADYLKKSTKFIFFNDVNKVGITINKYTKKGDFILVKGSRNWQLESILKSID

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=462, Percent_Identity=26.8398268398268, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51988; Mature: 51988

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFSVCNINTILGNLKNSENLKNQSQTFKNISLDSRNILNNDLFIAIKGKNFDGHDFLKE
CCCEEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
VLKKGVKAVVIRDGMQHLLPEKFPYWLVPNTLESFQKLALHKRRTLNIPVVGITGSVGKT
HHHCCCEEEEEHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
TTKEMMGEVLKQLGKIKLTQLNYNNEIGVGRTILDSDFKDKILIIEMGMRGLGQIENLSK
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHH
FSEPDIAVITNIGSSHIGILGSKENITYAKCEITKYLKPSGVVIIPANDLFLEETLKKNW
CCCCCEEEEEECCCCCEEEEECCCCCEEEEEEEEEEECCCCEEEECCCHHHHHHHHHCCC
SGRIIKVDLLDINQKVDSINKKNLIGFFNRSNNSIIIEDKLFEISLKGFHNAFNFLFVYA
CCCEEEEEEEECCHHHHCCCCCCEEEEEECCCCEEEEEEEEEEEEEHHHHHHHHHHHHHH
VAKEFGIEFKDTNKFNFLTLDGRNKIIKSKKTVIYDETYNASPESLKACIINLLNNPNNH
HHHHHCCEEECCCCEEEEEECCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCE
FLIFGSIQELGSKSKKYHKELFDFIVKSDIKKCIFICDRNDEIYYADYLKKSTKFIFFND
EEEEECHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHCCCCEEEEEC
VNKVGITINKYTKKGDFILVKGSRNWQLESILKSID
CCEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHCC
>Mature Secondary Structure
MDFSVCNINTILGNLKNSENLKNQSQTFKNISLDSRNILNNDLFIAIKGKNFDGHDFLKE
CCCEEEEHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHH
VLKKGVKAVVIRDGMQHLLPEKFPYWLVPNTLESFQKLALHKRRTLNIPVVGITGSVGKT
HHHCCCEEEEEHHHHHHHCCCCCCEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
TTKEMMGEVLKQLGKIKLTQLNYNNEIGVGRTILDSDFKDKILIIEMGMRGLGQIENLSK
HHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCHHHHHH
FSEPDIAVITNIGSSHIGILGSKENITYAKCEITKYLKPSGVVIIPANDLFLEETLKKNW
CCCCCEEEEEECCCCCEEEEECCCCCEEEEEEEEEEECCCCEEEECCCHHHHHHHHHCCC
SGRIIKVDLLDINQKVDSINKKNLIGFFNRSNNSIIIEDKLFEISLKGFHNAFNFLFVYA
CCCEEEEEEEECCHHHHCCCCCCEEEEEECCCCEEEEEEEEEEEEEHHHHHHHHHHHHHH
VAKEFGIEFKDTNKFNFLTLDGRNKIIKSKKTVIYDETYNASPESLKACIINLLNNPNNH
HHHHHCCEEECCCCEEEEEECCCCCEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCE
FLIFGSIQELGSKSKKYHKELFDFIVKSDIKKCIFICDRNDEIYYADYLKKSTKFIFFND
EEEEECHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEHHHHHCCCCEEEEEC
VNKVGITINKYTKKGDFILVKGSRNWQLESILKSID
CCEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]