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The map label for this gene is birA [H]

Identifier: 123965730

GI number: 123965730

Start: 441976

End: 442725

Strand: Direct

Name: birA [H]

Synonym: P9515_04951

Alternate gene names: 123965730

Gene position: 441976-442725 (Clockwise)

Preceding gene: 123965728

Following gene: 123965733

Centisome position: 25.93

GC content: 29.6

Gene sequence:

>750_bases
GTGAAAGTTTTAGGTTCTGTAGCTAGGACAGCATTTTATTTCAAAAAAATTCAGGGATTGTGCCCAGATTGGAAACTGCA
ATACAAATTCAAATGTAGAAGTACTGAATATGAACTAACAAATTTGCTTCAAGATTCTCCTGTCCAGAGAAATAAACCAA
TAGCAATAGTGGCCAAAGAGCAATTATCAGGGATAGGTCAAAATTCAAGATCATGGTTTTCTCCTAAAGGAGGAATTTGG
CTTAGTGCAGCTTATCCAATATTCTCCTCAGAATTTTCAAGTGAGACTTTGAGTCTTTCATTCGCAATTAAGTTATGTGA
AATGTTTCAAATGGAATCTATAAATGTTGATTTGAAATGGCCAAATGATATTTTTTATGATTCTAAAAAATTAATTGGAT
TTTTACCTAGGGTCATAACAAGAGGAAAAGAAATTATTTATATGAGAATTGGTTTGGGAATGAATTTTTCAAATAAAACT
CCATTAGAGGGGATTGCTTTATCAGAAATACTTAAAACTAAAAATATATGCGAACATTATTGGACTGCGAAAATTCTTAA
AACTATTCATGATTCAGTCGAATTTAATGGTAAAAAAGAATATATTATTGAGAATGCAAATAAATACCTTACAAAAAAAC
AATTACCTAGAGGGTATAACTCCAAGTACTGGAAAATAAAAGATGTAGACAATAATGGGAATTTGAGAATTTATAATCAA
ACCCAAGAAAAAGTATTAAAAAGATTTTAA

Upstream 100 bases:

>100_bases
TATTGGTAAATATTTCTTATTCAATTTGTTTAATTAAAACTATAAGATTTAAGATAATTTAATCTATTAAAGATTGCTTG
TTTTTTAATATTAAGGTTTT

Downstream 100 bases:

>100_bases
ATTTATTTTATTTTTAATTCAACTATTTTCCCATCTTTAAACTTTGCAACTTTTTTTGCACGACTTGCAACTTCATCTTC
GTGAGTTACTAAAACTATAG

Product: putative biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKVLGSVARTAFYFKKIQGLCPDWKLQYKFKCRSTEYELTNLLQDSPVQRNKPIAIVAKEQLSGIGQNSRSWFSPKGGIW
LSAAYPIFSSEFSSETLSLSFAIKLCEMFQMESINVDLKWPNDIFYDSKKLIGFLPRVITRGKEIIYMRIGLGMNFSNKT
PLEGIALSEILKTKNICEHYWTAKILKTIHDSVEFNGKKEYIIENANKYLTKKQLPRGYNSKYWKIKDVDNNGNLRIYNQ
TQEKVLKRF

Sequences:

>Translated_249_residues
MKVLGSVARTAFYFKKIQGLCPDWKLQYKFKCRSTEYELTNLLQDSPVQRNKPIAIVAKEQLSGIGQNSRSWFSPKGGIW
LSAAYPIFSSEFSSETLSLSFAIKLCEMFQMESINVDLKWPNDIFYDSKKLIGFLPRVITRGKEIIYMRIGLGMNFSNKT
PLEGIALSEILKTKNICEHYWTAKILKTIHDSVEFNGKKEYIIENANKYLTKKQLPRGYNSKYWKIKDVDNNGNLRIYNQ
TQEKVLKRF
>Mature_249_residues
MKVLGSVARTAFYFKKIQGLCPDWKLQYKFKCRSTEYELTNLLQDSPVQRNKPIAIVAKEQLSGIGQNSRSWFSPKGGIW
LSAAYPIFSSEFSSETLSLSFAIKLCEMFQMESINVDLKWPNDIFYDSKKLIGFLPRVITRGKEIIYMRIGLGMNFSNKT
PLEGIALSEILKTKNICEHYWTAKILKTIHDSVEFNGKKEYIIENANKYLTKKQLPRGYNSKYWKIKDVDNNGNLRIYNQ
TQEKVLKRF

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004408
- InterPro:   IPR004409
- InterPro:   IPR003142
- InterPro:   IPR004143
- InterPro:   IPR008988 [H]

Pfam domain/function: PF02237 BPL_C; PF03099 BPL_LipA_LipB [H]

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 28928; Mature: 28928

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLGSVARTAFYFKKIQGLCPDWKLQYKFKCRSTEYELTNLLQDSPVQRNKPIAIVAKE
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEHH
QLSGIGQNSRSWFSPKGGIWLSAAYPIFSSEFSSETLSLSFAIKLCEMFQMESINVDLKW
HHHCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEC
PNDIFYDSKKLIGFLPRVITRGKEIIYMRIGLGMNFSNKTPLEGIALSEILKTKNICEHY
CCCHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WTAKILKTIHDSVEFNGKKEYIIENANKYLTKKQLPRGYNSKYWKIKDVDNNGNLRIYNQ
HHHHHHHHHHHHHCCCCCHHEEEECCHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEC
TQEKVLKRF
HHHHHHHCC
>Mature Secondary Structure
MKVLGSVARTAFYFKKIQGLCPDWKLQYKFKCRSTEYELTNLLQDSPVQRNKPIAIVAKE
CCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEHH
QLSGIGQNSRSWFSPKGGIWLSAAYPIFSSEFSSETLSLSFAIKLCEMFQMESINVDLKW
HHHCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEC
PNDIFYDSKKLIGFLPRVITRGKEIIYMRIGLGMNFSNKTPLEGIALSEILKTKNICEHY
CCCHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WTAKILKTIHDSVEFNGKKEYIIENANKYLTKKQLPRGYNSKYWKIKDVDNNGNLRIYNQ
HHHHHHHHHHHHHCCCCCHHEEEECCHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEEC
TQEKVLKRF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]