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The map label for this gene is topA [H]

Identifier: 123965733

GI number: 123965733

Start: 445118

End: 447730

Strand: Direct

Name: topA [H]

Synonym: P9515_04981

Alternate gene names: 123965733

Gene position: 445118-447730 (Clockwise)

Preceding gene: 123965730

Following gene: 123965734

Centisome position: 26.12

GC content: 35.36

Gene sequence:

>2613_bases
TTGGATCACACACTTGTTATTGTTGAAAGTCCCACAAAGGCAAAAACTATAAGAAGGTTTTTGCCTCCTAATTATGAAGT
TTTAGCTTCTATGGGGCATGTAAGAGATCTTCCAAAAGGAGCTGCTGAAATCCCTGCATCAGTAAAAAAAGAAAAATGGT
CAAGAATAGGTGTAAACACTACAGAAGATTTTGAACCACTTTATATCGTTCCTAAAGAAAAAAAGAAAGTTGTTAAAGAT
TTAAAAAATGCCTTAAAAGATGCTACCCAATTACTATTAGCAACTGATGAAGATAGGGAGGGAGAGAGTATAAGTTGGCA
CTTAATGCAAATACTTAAACCCAAGATTCCAACTAAACGGATGGTTTTTCATGAAATTACTAAAAAGGCTATTAATAAGG
CTCTAGATGAAACTAGAGAAATTGATATGGAATTAGTTCAGGCCCAAGAAACAAGAAGAATTCTTGACAGGCTTTTTGGA
TATGAACTTTCCCCATTACTTTGGAAAAAGGTAGCCCCTAGGCTTTCTGCAGGTCGTGTTCAGTCTGTATCTGTAAGATT
GCTTGTTAATAAAGAAAGAGAAAGGAGAGCTTTTAAAAAAGCTACTTATTGGGGATTAAAAGCCACTTTACTTAAGGATA
ATGTTTTTTTTGACAGTAAGTTATCTAGCCTATCTGGTCAGAAAATCTCTAACGGATCTGATTTTGATGAGAAAACCGGT
AAATTAAAAAATGGTAATAAATCTTTAATTTTGAACGAAGAGAGAGCGAAGAGTTTGCTCAAGTCTTTATCTGAAGAAAA
GTGGAGAGTTATAAAAATAGAGAAAAAACCAACAACTAGGAAGCCTGTTCCACCATTTACTACTAGTACCTTGCAGCAGG
AGGCTAATCGAAAACTTCGATTATCAGCAAGAGAAACAATGAGATGTGCACAAGGCTTGTATGAGAGAGGTTTTATTACA
TATATGAGAACTGATTCGGTACATCTCTCAGAACAAGCAATAAGAGCGGCGAGAGAATGTGTGCAGTCAAGATACGGAAA
AGAATATTTATCAAGTTCAGTTCGTCAATTTAATTCAAAAGAAAGGAATGCTCAAGAAGCTCATGAAGCTATTAGACCTG
CCGGAGAAGTATTTAAAACACCCAGCGATACAGACTTGGCGGGAAGGGATCTTTCCCTATATGAATTAATTTGGAAAAGA
ACTGTTGCCAGTCAAATGGCTGAAGCAAGATTAACTATGGTTAATGCTGAAATAGAAGTGGGAGAAGGATTATTTAAGTC
CAGTGGAAAAAGTATTGATTTTGCAGGATTTTTTAGAGCTTACGTCGAAGGTAGTGATGATCCAAGTGCATCATTGGAAC
AGCAAGAAGTAATTCTCCCAAATTTAACTCTTGGGTCTACTCTTGAGGTGGCCAGTAAAGAGGCTACTTATCATGAGACT
AAATCTCCAGCCAGATATACTGAGGCTGCATTAGTTAAAGTTCTAGAAAAAGAGGGCATAGGCAGACCTTCTACTTATGC
AAGTATTATTGGAACAATAGTTGATAGGGGTTATGCAAATATTTCTTCAAACTCTTTATCTCCTACCTTTACTGCTTTTG
CTGTTACGGCATTACTAGAGGAGCACTTCCCTGATCTAGTAGATACTACTTTTACGGCAAAAATGGAATCTTCATTAGAT
GAAATTTCTTCAGGTAATCTAGAATGGCTACCATATTTAGAAACTTTTTATAAAGGTAAAAACGGTCTTGAGGTGAAAGT
TCAGAAAACAGAAGGTGATATTGATGGGAAAGCATATAGACAAGTTGATTTTGATGATCTCCCTTGTGTAGTTAGAATCG
GATCAAACGGGCCATGGCTAGAGGGAGTAAAAATAGATGAATCAGGAAATGAAATACAAGCAAAAGGAAATCTCCCTATG
GATATTACTCCTGGAGATTTAGATAAAAAGAAAGTTGATCAAATACTAAGTGGCCCCTCAGATCTTGGGACTGATCCAAA
AACAGGTGAGCAAGTATTTTTGAGATTTGGTCCTTATGGACCTTATGTTCAGCTAGGTAATATTGAAGAGGGTAAAGCCA
AGCCAAGAAGGGCTAGTTTACCCAAAGACTTGAAAACTGATGACTTGTCATTATCGGAAGCTCTAGAACTATTAAGCTTA
CCAAAATTGCTTGGAGAGCATCCCGAGGGTGGAATTGTTGAGGCTGACAGAGGAAGATTTGGTCCTTATATTAAATGGAT
CAAAGATGAAGATACTTCTGAAAATAGATCTTTAAAAAAAGAAGATGATGTATTTAAGGTTGATCTTAAGCGTGCATTAG
AAATCCTCGCGATGCCAAAATTAGGGAGAGGGGGACAAGAAGTAATAAAGGATTTTGGGAAACCTAAAGAGTTAAATGAT
AAAGTTCAAGTTTTAAATGGAAAATATGGAATATATGTGAAGTGTGGGAAAATAAATGTTTCTCTGCCTAAAGATACTGA
TTTAGAAAAATTTACAATTGAAAATGCCTTGATTCTTTTAGAAGAGAAAATGAAAGATAAAAAGGTTTCTGTTTTCAAAA
AAAATAAAGTAATTAGTAAAAAGACCAAAAAAAATAAAAAAATTAAAAAATAA

Upstream 100 bases:

>100_bases
ATGATTTTTGAAATTCTTGAGCAAGTTTGTTTTAAGAAGTTTCAATTAATCATAATATGCCCTAATTGATTAAAAAAACA
CCAATTATTTGAAATAGACC

Downstream 100 bases:

>100_bases
ATATGGGTTTTAAAATAAAAAAAGTTTTTTTATTGATAATTTTATTTTTATTGCAATCATGCTCGGGAGGGAGAGTTGGA
GATTTCTTAGAATCTAGTTT

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 870; Mature: 870

Protein sequence:

>870_residues
MDHTLVIVESPTKAKTIRRFLPPNYEVLASMGHVRDLPKGAAEIPASVKKEKWSRIGVNTTEDFEPLYIVPKEKKKVVKD
LKNALKDATQLLLATDEDREGESISWHLMQILKPKIPTKRMVFHEITKKAINKALDETREIDMELVQAQETRRILDRLFG
YELSPLLWKKVAPRLSAGRVQSVSVRLLVNKERERRAFKKATYWGLKATLLKDNVFFDSKLSSLSGQKISNGSDFDEKTG
KLKNGNKSLILNEERAKSLLKSLSEEKWRVIKIEKKPTTRKPVPPFTTSTLQQEANRKLRLSARETMRCAQGLYERGFIT
YMRTDSVHLSEQAIRAARECVQSRYGKEYLSSSVRQFNSKERNAQEAHEAIRPAGEVFKTPSDTDLAGRDLSLYELIWKR
TVASQMAEARLTMVNAEIEVGEGLFKSSGKSIDFAGFFRAYVEGSDDPSASLEQQEVILPNLTLGSTLEVASKEATYHET
KSPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNSLSPTFTAFAVTALLEEHFPDLVDTTFTAKMESSLD
EISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYRQVDFDDLPCVVRIGSNGPWLEGVKIDESGNEIQAKGNLPM
DITPGDLDKKKVDQILSGPSDLGTDPKTGEQVFLRFGPYGPYVQLGNIEEGKAKPRRASLPKDLKTDDLSLSEALELLSL
PKLLGEHPEGGIVEADRGRFGPYIKWIKDEDTSENRSLKKEDDVFKVDLKRALEILAMPKLGRGGQEVIKDFGKPKELND
KVQVLNGKYGIYVKCGKINVSLPKDTDLEKFTIENALILLEEKMKDKKVSVFKKNKVISKKTKKNKKIKK

Sequences:

>Translated_870_residues
MDHTLVIVESPTKAKTIRRFLPPNYEVLASMGHVRDLPKGAAEIPASVKKEKWSRIGVNTTEDFEPLYIVPKEKKKVVKD
LKNALKDATQLLLATDEDREGESISWHLMQILKPKIPTKRMVFHEITKKAINKALDETREIDMELVQAQETRRILDRLFG
YELSPLLWKKVAPRLSAGRVQSVSVRLLVNKERERRAFKKATYWGLKATLLKDNVFFDSKLSSLSGQKISNGSDFDEKTG
KLKNGNKSLILNEERAKSLLKSLSEEKWRVIKIEKKPTTRKPVPPFTTSTLQQEANRKLRLSARETMRCAQGLYERGFIT
YMRTDSVHLSEQAIRAARECVQSRYGKEYLSSSVRQFNSKERNAQEAHEAIRPAGEVFKTPSDTDLAGRDLSLYELIWKR
TVASQMAEARLTMVNAEIEVGEGLFKSSGKSIDFAGFFRAYVEGSDDPSASLEQQEVILPNLTLGSTLEVASKEATYHET
KSPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNSLSPTFTAFAVTALLEEHFPDLVDTTFTAKMESSLD
EISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYRQVDFDDLPCVVRIGSNGPWLEGVKIDESGNEIQAKGNLPM
DITPGDLDKKKVDQILSGPSDLGTDPKTGEQVFLRFGPYGPYVQLGNIEEGKAKPRRASLPKDLKTDDLSLSEALELLSL
PKLLGEHPEGGIVEADRGRFGPYIKWIKDEDTSENRSLKKEDDVFKVDLKRALEILAMPKLGRGGQEVIKDFGKPKELND
KVQVLNGKYGIYVKCGKINVSLPKDTDLEKFTIENALILLEEKMKDKKVSVFKKNKVISKKTKKNKKIKK
>Mature_870_residues
MDHTLVIVESPTKAKTIRRFLPPNYEVLASMGHVRDLPKGAAEIPASVKKEKWSRIGVNTTEDFEPLYIVPKEKKKVVKD
LKNALKDATQLLLATDEDREGESISWHLMQILKPKIPTKRMVFHEITKKAINKALDETREIDMELVQAQETRRILDRLFG
YELSPLLWKKVAPRLSAGRVQSVSVRLLVNKERERRAFKKATYWGLKATLLKDNVFFDSKLSSLSGQKISNGSDFDEKTG
KLKNGNKSLILNEERAKSLLKSLSEEKWRVIKIEKKPTTRKPVPPFTTSTLQQEANRKLRLSARETMRCAQGLYERGFIT
YMRTDSVHLSEQAIRAARECVQSRYGKEYLSSSVRQFNSKERNAQEAHEAIRPAGEVFKTPSDTDLAGRDLSLYELIWKR
TVASQMAEARLTMVNAEIEVGEGLFKSSGKSIDFAGFFRAYVEGSDDPSASLEQQEVILPNLTLGSTLEVASKEATYHET
KSPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNSLSPTFTAFAVTALLEEHFPDLVDTTFTAKMESSLD
EISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYRQVDFDDLPCVVRIGSNGPWLEGVKIDESGNEIQAKGNLPM
DITPGDLDKKKVDQILSGPSDLGTDPKTGEQVFLRFGPYGPYVQLGNIEEGKAKPRRASLPKDLKTDDLSLSEALELLSL
PKLLGEHPEGGIVEADRGRFGPYIKWIKDEDTSENRSLKKEDDVFKVDLKRALEILAMPKLGRGGQEVIKDFGKPKELND
KVQVLNGKYGIYVKCGKINVSLPKDTDLEKFTIENALILLEEKMKDKKVSVFKKNKVISKKTKKNKKIKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=471, Percent_Identity=24.8407643312102, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI4507635, Length=582, Percent_Identity=22.680412371134, Blast_Score=105, Evalue=1e-22,
Organism=Escherichia coli, GI1787529, Length=593, Percent_Identity=40.1349072512648, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1788061, Length=532, Percent_Identity=22.5563909774436, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17555378, Length=523, Percent_Identity=25.0478011472275, Blast_Score=119, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=578, Percent_Identity=23.8754325259516, Blast_Score=117, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=529, Percent_Identity=22.6843100189036, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24585251, Length=480, Percent_Identity=26.6666666666667, Blast_Score=123, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97758; Mature: 97758

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHTLVIVESPTKAKTIRRFLPPNYEVLASMGHVRDLPKGAAEIPASVKKEKWSRIGVNT
CCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCC
TEDFEPLYIVPKEKKKVVKDLKNALKDATQLLLATDEDREGESISWHLMQILKPKIPTKR
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCCCHHH
MVFHEITKKAINKALDETREIDMELVQAQETRRILDRLFGYELSPLLWKKVAPRLSAGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCE
QSVSVRLLVNKERERRAFKKATYWGLKATLLKDNVFFDSKLSSLSGQKISNGSDFDEKTG
EEEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEECHHHHHCCCCCCCCCCCCHHHCC
KLKNGNKSLILNEERAKSLLKSLSEEKWRVIKIEKKPTTRKPVPPFTTSTLQQEANRKLR
CCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEE
LSARETMRCAQGLYERGFITYMRTDSVHLSEQAIRAARECVQSRYGKEYLSSSVRQFNSK
EHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERNAQEAHEAIRPAGEVFKTPSDTDLAGRDLSLYELIWKRTVASQMAEARLTMVNAEIEV
CCCHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECEEEC
GEGLFKSSGKSIDFAGFFRAYVEGSDDPSASLEQQEVILPNLTLGSTLEVASKEATYHET
CCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHHCCCHHHCC
KSPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNSLSPTFTAFAVTALLE
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
EHFPDLVDTTFTAKMESSLDEISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYR
HHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCEE
QVDFDDLPCVVRIGSNGPWLEGVKIDESGNEIQAKGNLPMDITPGDLDKKKVDQILSGPS
ECCCCCCCEEEEECCCCCEECCEEECCCCCEEEECCCCCEECCCCCCCHHHHHHHHCCCH
DLGTDPKTGEQVFLRFGPYGPYVQLGNIEEGKAKPRRASLPKDLKTDDLSLSEALELLSL
HCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
PKLLGEHPEGGIVEADRGRFGPYIKWIKDEDTSENRSLKKEDDVFKVDLKRALEILAMPK
HHHHCCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
LGRGGQEVIKDFGKPKELNDKVQVLNGKYGIYVKCGKINVSLPKDTDLEKFTIENALILL
CCCCHHHHHHHCCCCCCCCCHHEEECCCEEEEEEECEEEEECCCCCCCHHEEHHHHHHHH
EEKMKDKKVSVFKKNKVISKKTKKNKKIKK
HHHHCCHHHHHHHHCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MDHTLVIVESPTKAKTIRRFLPPNYEVLASMGHVRDLPKGAAEIPASVKKEKWSRIGVNT
CCCEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHCCCCC
TEDFEPLYIVPKEKKKVVKDLKNALKDATQLLLATDEDREGESISWHLMQILKPKIPTKR
CCCCCCEEEECCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHCCCCCHHH
MVFHEITKKAINKALDETREIDMELVQAQETRRILDRLFGYELSPLLWKKVAPRLSAGRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCE
QSVSVRLLVNKERERRAFKKATYWGLKATLLKDNVFFDSKLSSLSGQKISNGSDFDEKTG
EEEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCEECHHHHHCCCCCCCCCCCCHHHCC
KLKNGNKSLILNEERAKSLLKSLSEEKWRVIKIEKKPTTRKPVPPFTTSTLQQEANRKLR
CCCCCCCEEEECHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCEEE
LSARETMRCAQGLYERGFITYMRTDSVHLSEQAIRAARECVQSRYGKEYLSSSVRQFNSK
EHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ERNAQEAHEAIRPAGEVFKTPSDTDLAGRDLSLYELIWKRTVASQMAEARLTMVNAEIEV
CCCHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECEEEC
GEGLFKSSGKSIDFAGFFRAYVEGSDDPSASLEQQEVILPNLTLGSTLEVASKEATYHET
CCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCHHHHHCCCHHHCC
KSPARYTEAALVKVLEKEGIGRPSTYASIIGTIVDRGYANISSNSLSPTFTAFAVTALLE
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
EHFPDLVDTTFTAKMESSLDEISSGNLEWLPYLETFYKGKNGLEVKVQKTEGDIDGKAYR
HHCCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEEECCCCCCCCCCEE
QVDFDDLPCVVRIGSNGPWLEGVKIDESGNEIQAKGNLPMDITPGDLDKKKVDQILSGPS
ECCCCCCCEEEEECCCCCEECCEEECCCCCEEEECCCCCEECCCCCCCHHHHHHHHCCCH
DLGTDPKTGEQVFLRFGPYGPYVQLGNIEEGKAKPRRASLPKDLKTDDLSLSEALELLSL
HCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
PKLLGEHPEGGIVEADRGRFGPYIKWIKDEDTSENRSLKKEDDVFKVDLKRALEILAMPK
HHHHCCCCCCCEEECCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
LGRGGQEVIKDFGKPKELNDKVQVLNGKYGIYVKCGKINVSLPKDTDLEKFTIENALILL
CCCCHHHHHHHCCCCCCCCCHHEEECCCEEEEEEECEEEEECCCCCCCHHEEHHHHHHHH
EEKMKDKKVSVFKKNKVISKKTKKNKKIKK
HHHHCCHHHHHHHHCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7659748 [H]