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The map label for this gene is trpB [H]

Identifier: 123965428

GI number: 123965428

Start: 176827

End: 178071

Strand: Reverse

Name: trpB [H]

Synonym: P9515_01931

Alternate gene names: 123965428

Gene position: 178071-176827 (Counterclockwise)

Preceding gene: 123965431

Following gene: 123965426

Centisome position: 10.45

GC content: 38.39

Gene sequence:

>1245_bases
TTGGTAAGTACAATTTCCCGTCATGATCAAAATAAGAATAACGATTATTTGAATCAACCTTCGAAGGAAGGCAGGTTTGG
AAAGTATGGAGGTCAATATGTCCCTGAAACTTTAATGCCAGCTCTTTTTGAACTCGAAAAAGCCGCATCTGATGCCTGGA
AAGATAAACAATTTGTTGATGAATTAAATCATTTATTGAAAACTTATGTCGGGAGAGAGACACCACTATATGAAGCCAAA
AGACTTACTGAACATTACCAAACGAAAACCTGTACTAGTAGAATATGGCTAAAAAGAGAGGACCTTAATCATACTGGTGC
TCACAAAATCAATAACGCATTAGGACAAGCTTTATTAGCCATAAGAATGGGCAAGGAAAGAATAATTGCGGAAACAGGTG
CTGGGCAACACGGAGTAGCAACTGCTACTGTATGCGCGAGATTCGGTTTGAAATGCATTATCTACATGGGAGCGGAAGAT
ATAAAAAGACAATCATTAAACGTCTTTCGCATGAAACTTTTAGGAGCAGAAGTTAAAGTTGTCACTTCAGGTACTGCAAC
TCTCAAAGATGCGACTAGTGAAGCTATTAGAGATTGGGTATCCAATGTAGAAACTACACACTATATTTTAGGTTCTGTTG
CGGGACCACATCCATTCCCAATGATTGTTAGGGATTTTCATGCAGTCATAGGAGAAGAAGCGAAAAAACAATGTGTAGAA
TCGTTTGGATCACTACCTGATATATTGCTTGCTTGTGTGGGTGGAGGATCAAATGCAATGGGTCTTTTTCATCCCTTTGT
AAAAGAAAAATCAGTCCGACTAATTGGAGTTGAAGCTGCAGGAAGCGGAGTCAACACTGACAAACATGCCGCTACTATTA
CTAAAGGATCAGTTGGAATTTTACATGGATCAATGAGCCTTCTTTTACAAGATAAGAACGGTCAAGTTCAAGAAGCACAT
TCTATAAGCGCAGGTCTTGATTATCCCGGGGTAGGTCCTGAACATAGTTACTTAAAAGATATAGGAAGAGCAGAATATGG
CTCTGTTACTGATACAGAAGCTTTAAATGCTTTGAAACTTGTTAGTGAACTAGAAGGGATTATTCCTGCACTCGAAACAG
CTCATGCCTTTGCTTGGCTGGAGAAATTATGTCCTACTCTAGAAAAAAATACAGAAATAGTAATTAATTGTTCAGGCAGA
GGAGATAAAGATGTTAATACTGTTGCATCATCTTTGGATATTTAG

Upstream 100 bases:

>100_bases
TCTTGGTAACTATTTTCCTCATTAACAAACTCAATCCAATTTTTTTTTCCCATTATTTTTTATATATTCTATAGTTAGTT
AATACTTATACTATAGAAAA

Downstream 100 bases:

>100_bases
TAACTAATATCTAGGTATTGTTTGATCAATATACCTACTCCATCCATCTATACCTTCTTCTAGATTCCAAATCTCATTTA
CGAGATTATTATCTAATGAC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MVSTISRHDQNKNNDYLNQPSKEGRFGKYGGQYVPETLMPALFELEKAASDAWKDKQFVDELNHLLKTYVGRETPLYEAK
RLTEHYQTKTCTSRIWLKREDLNHTGAHKINNALGQALLAIRMGKERIIAETGAGQHGVATATVCARFGLKCIIYMGAED
IKRQSLNVFRMKLLGAEVKVVTSGTATLKDATSEAIRDWVSNVETTHYILGSVAGPHPFPMIVRDFHAVIGEEAKKQCVE
SFGSLPDILLACVGGGSNAMGLFHPFVKEKSVRLIGVEAAGSGVNTDKHAATITKGSVGILHGSMSLLLQDKNGQVQEAH
SISAGLDYPGVGPEHSYLKDIGRAEYGSVTDTEALNALKLVSELEGIIPALETAHAFAWLEKLCPTLEKNTEIVINCSGR
GDKDVNTVASSLDI

Sequences:

>Translated_414_residues
MVSTISRHDQNKNNDYLNQPSKEGRFGKYGGQYVPETLMPALFELEKAASDAWKDKQFVDELNHLLKTYVGRETPLYEAK
RLTEHYQTKTCTSRIWLKREDLNHTGAHKINNALGQALLAIRMGKERIIAETGAGQHGVATATVCARFGLKCIIYMGAED
IKRQSLNVFRMKLLGAEVKVVTSGTATLKDATSEAIRDWVSNVETTHYILGSVAGPHPFPMIVRDFHAVIGEEAKKQCVE
SFGSLPDILLACVGGGSNAMGLFHPFVKEKSVRLIGVEAAGSGVNTDKHAATITKGSVGILHGSMSLLLQDKNGQVQEAH
SISAGLDYPGVGPEHSYLKDIGRAEYGSVTDTEALNALKLVSELEGIIPALETAHAFAWLEKLCPTLEKNTEIVINCSGR
GDKDVNTVASSLDI
>Mature_414_residues
MVSTISRHDQNKNNDYLNQPSKEGRFGKYGGQYVPETLMPALFELEKAASDAWKDKQFVDELNHLLKTYVGRETPLYEAK
RLTEHYQTKTCTSRIWLKREDLNHTGAHKINNALGQALLAIRMGKERIIAETGAGQHGVATATVCARFGLKCIIYMGAED
IKRQSLNVFRMKLLGAEVKVVTSGTATLKDATSEAIRDWVSNVETTHYILGSVAGPHPFPMIVRDFHAVIGEEAKKQCVE
SFGSLPDILLACVGGGSNAMGLFHPFVKEKSVRLIGVEAAGSGVNTDKHAATITKGSVGILHGSMSLLLQDKNGQVQEAH
SISAGLDYPGVGPEHSYLKDIGRAEYGSVTDTEALNALKLVSELEGIIPALETAHAFAWLEKLCPTLEKNTEIVINCSGR
GDKDVNTVASSLDI

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=384, Percent_Identity=55.9895833333333, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6321412, Length=389, Percent_Identity=57.5835475578406, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 45002; Mature: 45002

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTISRHDQNKNNDYLNQPSKEGRFGKYGGQYVPETLMPALFELEKAASDAWKDKQFVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELNHLLKTYVGRETPLYEAKRLTEHYQTKTCTSRIWLKREDLNHTGAHKINNALGQALLA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHH
IRMGKERIIAETGAGQHGVATATVCARFGLKCIIYMGAEDIKRQSLNVFRMKLLGAEVKV
HHHCCHHEEEECCCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCEEEE
VTSGTATLKDATSEAIRDWVSNVETTHYILGSVAGPHPFPMIVRDFHAVIGEEAKKQCVE
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
SFGSLPDILLACVGGGSNAMGLFHPFVKEKSVRLIGVEAAGSGVNTDKHAATITKGSVGI
HHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCHHEEEECCCEEE
LHGSMSLLLQDKNGQVQEAHSISAGLDYPGVGPEHSYLKDIGRAEYGSVTDTEALNALKL
EECCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHH
VSELEGIIPALETAHAFAWLEKLCPTLEKNTEIVINCSGRGDKDVNTVASSLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVSTISRHDQNKNNDYLNQPSKEGRFGKYGGQYVPETLMPALFELEKAASDAWKDKQFVD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELNHLLKTYVGRETPLYEAKRLTEHYQTKTCTSRIWLKREDLNHTGAHKINNALGQALLA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHH
IRMGKERIIAETGAGQHGVATATVCARFGLKCIIYMGAEDIKRQSLNVFRMKLLGAEVKV
HHHCCHHEEEECCCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHHHCCEEEE
VTSGTATLKDATSEAIRDWVSNVETTHYILGSVAGPHPFPMIVRDFHAVIGEEAKKQCVE
EECCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
SFGSLPDILLACVGGGSNAMGLFHPFVKEKSVRLIGVEAAGSGVNTDKHAATITKGSVGI
HHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEEEECCCCCCCCCHHEEEECCCEEE
LHGSMSLLLQDKNGQVQEAHSISAGLDYPGVGPEHSYLKDIGRAEYGSVTDTEALNALKL
EECCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHH
VSELEGIIPALETAHAFAWLEKLCPTLEKNTEIVINCSGRGDKDVNTVASSLDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA