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The map label for this gene is cca

Identifier: 121999109

GI number: 121999109

Start: 2557090

End: 2558337

Strand: Reverse

Name: cca

Synonym: Hhal_2331

Alternate gene names: 121999109

Gene position: 2558337-2557090 (Counterclockwise)

Preceding gene: 121999110

Following gene: 121999107

Centisome position: 95.52

GC content: 74.12

Gene sequence:

>1248_bases
GTGGAACGCTACCGGGTCGGCGGCGCGGTGCGTGATCGCCTCCTCGGGCGGCCCGTGGATGAGGTCGACTGGGTGGTGGT
CGGGGCGACGCCGGAGGCGATGGAGGCCGCCGGCTACCGGCCGGTGGGCAAGGATTTTCCGGTCTTCCTGCACCCGCAGA
CCGGCGAGGAGCACGCCCTGGCGCGCACCGAGCGCAAGACGGCGGTGGGCTATCACGGTTTCGAGTTCTACGCCGCGCCG
GAGGTGACCCTGGAGCAGGACCTGGCCCGCCGGGACCTGACCATCAACGCCATGGCCGAGGATGCCGGGGGCCGCCTCAT
CGACCCGTTCGGCGGCCAGGTCGACCTGCAGGCTCGTCAGCTGCGCCACGTCACCGACGCCTTCGCCGAGGACCCGGTGC
GCATTCTGCGCCTTGCGCGTTTTGCGGCCCGCTTCGCTGACGACGGCTTCGTCGTCGCCGACGAAACCATGGCCCTGTGC
CAGCGCATGGTCGCCGCCGGCGAGGTCGATGCCCTGGTCCCGGAGCGGGTGTGGCAGGAGCTCGCCCGGGGGCTGATGGA
GCCGACACCCCGTCGCATGATCGAGGTCCTGCGCCAGGCTGGTGCCCTGGCCCGTCTCCTCCCCGAGGTGGATGCCCTGT
TCGGTGTGCCGCAGTCGCCGGCCCACCACCCTGAGGGCGATGCCGGCACTCATACCCTCATGGCCCTGGATGCCGCGGCG
GCCGTCGACGCTTCGCTGGAGGCGCGCTTCGCCGTCCTGCTCCACGACGTGGGCAAGGCGCTCACCCCGGCGGAGGCGCT
CCCCCGCCACCCCGGCCACGAGGAGGCCGGCGTTGACGGCGCCCGGCGGGTCAGCGAGCGGCTGAAGGCGCCGCGGGCGT
GTCGGGATCTGGCCGTCCTGGTCACGCGCTGGCACATGCACGCCCACCGCGCGCTGGAGCTGCGCCCCGGCACGGTGGTG
GAACTGTTCGAGGGCCTGGACCTGTTCCGGCGCCCGGAGCGCCTGGAGCCGTTCCTGCACGCCTGCCTCGCGGACGATCG
TGGGCGCGGTGGCCGGGAGGCGAGCGACTGGCCGGCTGGCGACTTCCTGCGCGCCGCCTTCGAGGCGTGTCGCGGCGTTT
CGGCCCGGTCCTTCGTCGAGCAGGGCCTGCGCGGTCCTGAGGTGGGCGAGGCGGTTCGTGAGGCGCGCTGCCGGGCCGTG
GCCCAGGTGCCGCGCCCGGATGCCGGCGCCGCGCGGGACGGCGCCTAG

Upstream 100 bases:

>100_bases
CCGCGCCAACGCCCTGGAGGCGATCACCCCCGGGTACCTGGGTGCGGCCGGGCGGCAGGCGCAGTACCAGCAGGCCCGGC
GCGAGGCCGGTCGCTAGGCC

Downstream 100 bases:

>100_bases
GCGTGGCCCCTGGCTACAGCGTGGCGCGGCGGTCGGCCGGGGTGAAACCACCCAGGGGACCGTCGTAGTCGCGGCCCAGG
GCCATCACCTTGAACCGCTC

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MERYRVGGAVRDRLLGRPVDEVDWVVVGATPEAMEAAGYRPVGKDFPVFLHPQTGEEHALARTERKTAVGYHGFEFYAAP
EVTLEQDLARRDLTINAMAEDAGGRLIDPFGGQVDLQARQLRHVTDAFAEDPVRILRLARFAARFADDGFVVADETMALC
QRMVAAGEVDALVPERVWQELARGLMEPTPRRMIEVLRQAGALARLLPEVDALFGVPQSPAHHPEGDAGTHTLMALDAAA
AVDASLEARFAVLLHDVGKALTPAEALPRHPGHEEAGVDGARRVSERLKAPRACRDLAVLVTRWHMHAHRALELRPGTVV
ELFEGLDLFRRPERLEPFLHACLADDRGRGGREASDWPAGDFLRAAFEACRGVSARSFVEQGLRGPEVGEAVREARCRAV
AQVPRPDAGAARDGA

Sequences:

>Translated_415_residues
MERYRVGGAVRDRLLGRPVDEVDWVVVGATPEAMEAAGYRPVGKDFPVFLHPQTGEEHALARTERKTAVGYHGFEFYAAP
EVTLEQDLARRDLTINAMAEDAGGRLIDPFGGQVDLQARQLRHVTDAFAEDPVRILRLARFAARFADDGFVVADETMALC
QRMVAAGEVDALVPERVWQELARGLMEPTPRRMIEVLRQAGALARLLPEVDALFGVPQSPAHHPEGDAGTHTLMALDAAA
AVDASLEARFAVLLHDVGKALTPAEALPRHPGHEEAGVDGARRVSERLKAPRACRDLAVLVTRWHMHAHRALELRPGTVV
ELFEGLDLFRRPERLEPFLHACLADDRGRGGREASDWPAGDFLRAAFEACRGVSARSFVEQGLRGPEVGEAVREARCRAV
AQVPRPDAGAARDGA
>Mature_415_residues
MERYRVGGAVRDRLLGRPVDEVDWVVVGATPEAMEAAGYRPVGKDFPVFLHPQTGEEHALARTERKTAVGYHGFEFYAAP
EVTLEQDLARRDLTINAMAEDAGGRLIDPFGGQVDLQARQLRHVTDAFAEDPVRILRLARFAARFADDGFVVADETMALC
QRMVAAGEVDALVPERVWQELARGLMEPTPRRMIEVLRQAGALARLLPEVDALFGVPQSPAHHPEGDAGTHTLMALDAAA
AVDASLEARFAVLLHDVGKALTPAEALPRHPGHEEAGVDGARRVSERLKAPRACRDLAVLVTRWHMHAHRALELRPGTVV
ELFEGLDLFRRPERLEPFLHACLADDRGRGGREASDWPAGDFLRAAFEACRGVSARSFVEQGLRGPEVGEAVREARCRAV
AQVPRPDAGAARDGA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=412, Percent_Identity=52.9126213592233, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_HALHL (A1WZI2)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003896.1
- ProteinModelPortal:   A1WZI2
- SMR:   A1WZI2
- STRING:   A1WZI2
- GeneID:   4709280
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_2331
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   DRIWREW
- PhylomeDB:   A1WZI2
- BioCyc:   HHAL349124:HHAL_2331-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45348; Mature: 45348

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYRVGGAVRDRLLGRPVDEVDWVVVGATPEAMEAAGYRPVGKDFPVFLHPQTGEEHAL
CCCCCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCCHHHH
ARTERKTAVGYHGFEFYAAPEVTLEQDLARRDLTINAMAEDAGGRLIDPFGGQVDLQARQ
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCEEEEHHHCCCCCEECCCCCEECHHHHH
LRHVTDAFAEDPVRILRLARFAARFADDGFVVADETMALCQRMVAAGEVDALVPERVWQE
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHH
LARGLMEPTPRRMIEVLRQAGALARLLPEVDALFGVPQSPAHHPEGDAGTHTLMALDAAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AVDASLEARFAVLLHDVGKALTPAEALPRHPGHEEAGVDGARRVSERLKAPRACRDLAVL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
VTRWHMHAHRALELRPGTVVELFEGLDLFRRPERLEPFLHACLADDRGRGGREASDWPAG
HHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCH
DFLRAAFEACRGVSARSFVEQGLRGPEVGEAVREARCRAVAQVPRPDAGAARDGA
HHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MERYRVGGAVRDRLLGRPVDEVDWVVVGATPEAMEAAGYRPVGKDFPVFLHPQTGEEHAL
CCCCCCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEECCCCCCHHHH
ARTERKTAVGYHGFEFYAAPEVTLEQDLARRDLTINAMAEDAGGRLIDPFGGQVDLQARQ
HHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCEEEEHHHCCCCCEECCCCCEECHHHHH
LRHVTDAFAEDPVRILRLARFAARFADDGFVVADETMALCQRMVAAGEVDALVPERVWQE
HHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHH
LARGLMEPTPRRMIEVLRQAGALARLLPEVDALFGVPQSPAHHPEGDAGTHTLMALDAAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
AVDASLEARFAVLLHDVGKALTPAEALPRHPGHEEAGVDGARRVSERLKAPRACRDLAVL
HHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
VTRWHMHAHRALELRPGTVVELFEGLDLFRRPERLEPFLHACLADDRGRGGREASDWPAG
HHHHHHHHHHHEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCH
DFLRAAFEACRGVSARSFVEQGLRGPEVGEAVREARCRAVAQVPRPDAGAARDGA
HHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA